Efecto del pH y el peso inicial de implante sembrado en la multiplicación callogénica de canavalia ensiformis

Este trabajo evalúa el efecto del pH del medio de cultivo en la multiplicación callogénica de Canavalia ensiformis. Con el fin de obtener datos para un crecimiento óptimo de la biomasa callogénica, se determinaron dos rangos de trabajo, el primero comprendido entre 4.5 y 5.5 y el segundo entre 5.5 y...

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2014
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Universidad de Medellín
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Canavalia ensiformis
In vitro
Cultivo
pH
Canavalia ensiformis
In vitro
Cultures
pH
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dc.title.spa.fl_str_mv Efecto del pH y el peso inicial de implante sembrado en la multiplicación callogénica de canavalia ensiformis
Effect of pH and the initial culture weight on the callogenic multiplication of canavalia ensiformis
title Efecto del pH y el peso inicial de implante sembrado en la multiplicación callogénica de canavalia ensiformis
spellingShingle Efecto del pH y el peso inicial de implante sembrado en la multiplicación callogénica de canavalia ensiformis
Canavalia ensiformis
In vitro
Cultivo
pH
Canavalia ensiformis
In vitro
Cultures
pH
title_short Efecto del pH y el peso inicial de implante sembrado en la multiplicación callogénica de canavalia ensiformis
title_full Efecto del pH y el peso inicial de implante sembrado en la multiplicación callogénica de canavalia ensiformis
title_fullStr Efecto del pH y el peso inicial de implante sembrado en la multiplicación callogénica de canavalia ensiformis
title_full_unstemmed Efecto del pH y el peso inicial de implante sembrado en la multiplicación callogénica de canavalia ensiformis
title_sort Efecto del pH y el peso inicial de implante sembrado en la multiplicación callogénica de canavalia ensiformis
dc.subject.spa.fl_str_mv Canavalia ensiformis
In vitro
Cultivo
pH
Canavalia ensiformis
In vitro
Cultures
pH
topic Canavalia ensiformis
In vitro
Cultivo
pH
Canavalia ensiformis
In vitro
Cultures
pH
description Este trabajo evalúa el efecto del pH del medio de cultivo en la multiplicación callogénica de Canavalia ensiformis. Con el fin de obtener datos para un crecimiento óptimo de la biomasa callogénica, se determinaron dos rangos de trabajo, el primero comprendido entre 4.5 y 5.5 y el segundo entre 5.5 y 6.0, determinando el efecto que este producía en la multiplicación callogénica. Así mismo, se observó que la cantidad de callo sembrado inicialmente influye en el aumento de la biomasa. Luego de realizar un análisis de varianza a los datos de siembra obtenidos, se encontró que el mejor pH para el medio de cultivo es de 5.5, mientras que el peso del callo a ser sembrado debe ser mayor de 0.2500 gramos, con el fin de realizar una propagación adecuada de C. ensiformispara la obtención de metabolitos de interés comercial.
publishDate 2014
dc.date.created.none.fl_str_mv 2014
dc.date.accessioned.none.fl_str_mv 2017-06-15T22:05:20Z
dc.date.available.none.fl_str_mv 2017-06-15T22:05:20Z
dc.type.eng.fl_str_mv Article
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http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.identifier.citation.spa.fl_str_mv Saldarriaga, J. F.; Lopez, J.; Lopera, C.; Botero, L. (2014). Efecto del pH y el peso inicial de implante sembrado en la multiplicación callogénica de canavalia ensiformis. Avances en Ciencias e Ingeniería, 5(2), 73-83
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/11407/3425
dc.identifier.eissn.none.fl_str_mv 07188706
identifier_str_mv Saldarriaga, J. F.; Lopez, J.; Lopera, C.; Botero, L. (2014). Efecto del pH y el peso inicial de implante sembrado en la multiplicación callogénica de canavalia ensiformis. Avances en Ciencias e Ingeniería, 5(2), 73-83
07188706
url http://hdl.handle.net/11407/3425
dc.language.iso.none.fl_str_mv spa
language spa
dc.relation.isversionof.spa.fl_str_mv http://www.redalyc.org/html/3236/323631115006/
dc.relation.ispartofes.spa.fl_str_mv Avances en Ciencias e Ingeniería, vol. 5, núm. 2, abril-junio, 2014, pp. 73-83
dc.relation.references.spa.fl_str_mv Barjafidalgo, C., Guimaraes, J.A. & Carlini, C.R. (1991). Lipoxygenase-mediated secretory effect of canatoxin, the toxic protein from canavalia-ensiformisseeds. Toxicon, 29,453-459.
Becker-Ritt, A.B., Martinelli, A.H.S., Mitidieri, S., Feder, V., Wassermann, G.E., Santi, L., et al. (2007). Antifungal activity of plant and bacterial ureases. Toxicon, 50,971-983.
Bennett, I.J., Mcdavid, D.A.J. & Mccomb, J.A. (2003). The influence of ammonium nitrate, pH and indole butyric acid on root induction and survival in soil of micropropagated Eucalyptus globulus. Biologia Plantarum, 47,355-360.
Carlini, C.R. & Grossi-De-Sa, M.F. (2002). Plant toxic proteins with insecticidal properties. A review on their potentialities as bioinsecticides. Toxicon, 40, 1515-1539.
Carlini, C.R., Oliveira, A.E.A., Azambuja, P., Xavier, J. & Wells, M.A. (1997). Biological effects of canatoxin in different insect models: Evidence for a proteolytic activation of the toxin by insect cathepsinlike enzymes. Journal of Economic Entomology, 90,340-348.
Carlini, C.R. & Polacco, J.C. (2008). Toxic properties of urease. Crop Science, 48, 1665-1672.
Chaillou, S., Vessey, J.K., Morotgaudry, J.F., Raper, C.D., Henry, L.T. & Boutin, J.P. (1991). Expression of characteristics of ammonium nutrition as affected by ph of the root medium. Journal of Experimental Botany, 42,189-196.
Debergh, P.C. (1983). Effects of agar brand and concentration on the tissue-culture medium. Physiologia Plantarum, 59, 270-276.
FAO (2005). Some commonly used cover crop species. Food and Agriculture Organization of the United Nations. http://www.fao.org/ag/ca/2b.html, acceso 30/11/2013. 10. Ferreira-Dasilva, C.T., Gombarovits, M.E.C., Masuda, H., Oliveira, C.M. & Carlini, C.R. (2000). Proteolytic activation of canatoxin, a plant toxic protein, by insect Cathepsin-like enzymes. Archives of Insect Biochemistry and Physiology, 44, 162-171.
Follmer, C., Barcellos, G.B.S., Zingali, R.B., Machado, O.L.T., Alves, E.W., Barja-Fidalgo, C., et al. (2001). Canatoxin, a toxic protein from jack beans (Canavalia ensiformis), is a variant form of urease (EC 3.5.1.5): biological effects of urease independent of its ureolytic activity. Biochemical Journal, 360, 217-224.
Follmer, C., Real-Guerra, R., Wasserman, G.E., Olivera-Severo, D. & Carlini, C.R. (2004). Jackbean, soybean and Bacillus pasteurii ureases - Biological effects unrelated to ureolytic activity. European Journal of Biochemistry, 271, 1357-1363.
Goff, W.L. & Yunker, C.E. (1988). Effects of ph, buffers and medium-storage on the growth of babesia-bovis invitro. International Journal for Parasitology, 18, 775-778.
Hwang, D., Kim, S.G. & Kwon, Y.M. (1996). Canavanine synthesis in the in vitro propagated tissues of Canavalia lineata. Plant Cell Reports, 16,180-183.
Kovacs, P. (1982). Influence of different fixing agents on lectin binding by the tetrahymena. Acta Histochemica, 71, 245-252.
Lang, B. & Kaiser, W.M. (1994). Solute content and energy status of roots of barley plants cultivated at different ph on nitrate-nitrogen or ammonium-nitrogen. New Phytologist, 128,451-459.
Leifert, C., Pryce, S., Lumsden, P.J. & Waites, W.M. (1992). Effect of medium acidity on growth and rooting of different plant species growing in vitro. Plant Cell, Tissue and Organ Culture, 30(3), 171-179.
Mamiya, G., Takishima, K., Masakuni, M., Kayumi, T. & Ogawa, K. (1987). Complete amino-acid-sequence of jack bean urease. Journal of Protein Chemistry, 6, 55-59.
Mamiya, G., Takishima, K., Masakuni, M., Kayumi, T., Ogawa, K. & Sekita, T. (1985). Complete amino-acid sequence of jack bean urease. Proceedings of the Japan Academy Series B-Physical and Biological Sciences, 61,395-398.
Mulinari, F., Becker-Ritt, A.B., Demartini, D.R., Ligabue-Braun, R., Staniscuaski, F., Verli, H., et al. (2011). Characterization of JBURE-IIb isoform of Canavalia ensiformis (L.) DC urease. Biochimica Et Biophysica Acta-Proteins and Proteomics, 1814(12), 1758-1768.
Mulinari, F., Staniscuaski, F., Bertholdo-Vargas, L.R., Postal, M., Oliveira-Neto, O.B., Riden, D.J., et al. (2007). Jaburetox-2Ec: An insecticidal peptide derived from an isoform of urease from the plant Canavalia ensiformis . Peptides, 28, 2042-2050. 22. Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco cultures. Physioliologia Plantarum, 15, 473-497.
Orlikowska, T. (1992). Effects of mineral-composition and acidity of media, saccharose level, brand and quantity of agar on rooting of fruit rootstocks invitro. Biologia Plantarum, 34, 45-52.
Pasqua, G., Manes, F., Monacelli, B., Natale, L. & Anselmi, S. (2002). Effects of the culture medium pH and ion uptake in in vitro vegetative organogenesis in thin cell layers of tobacco. Plant Science, 162, 947-955.
Piovesan, A.R., Staniscuaski, F., Marco-Salvadori, J., Real-Guerra, R., Defferrari, M.S. & Carlini, C.R. (2008). Stage-specific gut proteinases of the cotton stainer bug Dysdercus peruvianus: Role in the release of entomotoxic peptides from Canavalia ensiformis urease. Insect Biochemistry and Molecular Biology, 38, 1023-1032.
Pires-Alves, M., Grossi-De-Sa, M.F., Barcellos, G.B.S., Carlini, C.R. & Moraes, M.G. (2003). Characterization and expression of a novel member (JBURE-II) of the urease gene family from jackbean Canavalia ensiformis (L.) DC. Plant and Cell Physiology, 44, 139-145.
Postal, M., Martinelli, A.H.S., Becker-Ritt, A.B., Ligabue-Braun, R., Demartini, D.R., Ribeiro, S.F.F., et al. (2012). Antifungal properties of Canavalia ensiformis urease and derived peptides. Peptides, 38, 22-32.
Raven, J.A. & Smith, F.A. (1976). Nitrogen assimilation and transport in vascular land plants in relation to intracellular ph regulation. New Phytologist, 76, 415-431.
Ribeirodasilva, G., Carlini, C.R., Piresbarbosa, R. & Guimaraes, J.A. (1986). Blood-glucose alterations induced in rats by canatoxin, a protein isolated from jack bean (canavalia-ensiformis) seeds. Toxicon, 24, 775-782.
Ribeirodasilva, G. & Prado, J.F. (1993). Increased insulin circulating levels induced by canatoxin in rats. Toxicon, 31,1131-1136.
Riddles, P.W., Whan, V., Blakeley, R.L. & Zerner, B. (1991). Cloning and sequencing of a jack bean urease-encoding cdna. Gene, 108,265-267.
Sathyanarayana, B.N. & Blake, J. (1994). The effect of nitrogen sources and initial ph of the media with or without buffer on invitro rooting of jackfruit (artocarpus-heterophyllus lam). Physiology, growth and development of plants in culture Conference entitled "Physiology, growth and development of plants and cells in culture", Physiology, growth and development of plants in culture. Kluwer, pp. 77-82.
Sato, A., Barcellos, G.B.S., Riedel, E.C., Carneiro, J.A., Carlini, C.R. & Esquibel, M.A. (1993). The presence of concanavalin-a and canatoxin in canavalia-ensiformisdc tissue-culture. Plant Cell Reports, 12,233-236.
Schenk, N., Hsiao, K.C. & Bornman, C.H. (1991). Avoidance of precipitation and carbohydrate breakdown in autoclaved plant-tissue culture media. Plant Cell Reports, 10,115-119.
Skirvin, R.M., Mcpheeters, K.D. & Norton, M. (1994). Sources and frequency of somaclonal variation. Hortscience, 29, 1232-1237.
Sridhar, K.R. & Seena, S. (2006). Nutritional and antinutritional significance of four unconventional legumes of the genus Canavalia - A comparative study. Food Chemistry, 99, 267-288.
Staniscuaski, F., Brugge, V.T., Carlini, C.R. & Orchard, I. (2009). In vitro effect of Canavalia ensiformis urease and the derived peptide Jaburetox-2Ec on Rhodnius prolixus Malpighian tubules. Journal of Insect Physiology, 55 , 255-263.
Tan, X.W., Ikeda, H. & Oda, M. (2000). The absorption, translocation, and assimilation of urea, nitrate or ammonium in tomato plants at different plant growth stages in hydroponic culture. Scientia Horticulturae, 84, 275-283.
Woodward, A.J., Bennett, I.J. & Pusswonge, S. (2006). The effect of nitrogen source and concentration, medium pH and buffering on in vitro shoot growth and rooting in Eucalyptus marginata. Scientia Horticulturae, 110, 208-213.
Zsoldos, F. & Haunold, E. (1982). Influence of 2, 4-d and low ph on potassium, ammonium and nitrate uptake by rice roots. Physiologia Plantarum, 54, 63-68.
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spelling 2017-06-15T22:05:20Z2017-06-15T22:05:20Z2014Saldarriaga, J. F.; Lopez, J.; Lopera, C.; Botero, L. (2014). Efecto del pH y el peso inicial de implante sembrado en la multiplicación callogénica de canavalia ensiformis. Avances en Ciencias e Ingeniería, 5(2), 73-83http://hdl.handle.net/11407/342507188706Este trabajo evalúa el efecto del pH del medio de cultivo en la multiplicación callogénica de Canavalia ensiformis. Con el fin de obtener datos para un crecimiento óptimo de la biomasa callogénica, se determinaron dos rangos de trabajo, el primero comprendido entre 4.5 y 5.5 y el segundo entre 5.5 y 6.0, determinando el efecto que este producía en la multiplicación callogénica. Así mismo, se observó que la cantidad de callo sembrado inicialmente influye en el aumento de la biomasa. Luego de realizar un análisis de varianza a los datos de siembra obtenidos, se encontró que el mejor pH para el medio de cultivo es de 5.5, mientras que el peso del callo a ser sembrado debe ser mayor de 0.2500 gramos, con el fin de realizar una propagación adecuada de C. ensiformispara la obtención de metabolitos de interés comercial.This paper evaluates the effect of pH of the culture medium in Canavalia ensiformiscallogenic multiplication. In order to obtain data for optimal growth of biomass callogenic, two work ranges were determined, the first between 4.5 and 5.5 and second between 5.5 and 6.0, determining the effect it produced on callogenic multiplication. Also, it was observed that the amount of seed callus affects the increase of biomass. Then an analysis of variance to planting obtained data , it was found that the best pH for the culture medium is 5.5, whereas the weight of the callus to be sown should be greater than 0.2500 grams, in order to realize was made C. ensiformis propagation adequate for commercial production of metabolites of interest.spaExecutive Business School ChileIngeniería AmbientalFacultad de Ingenieríashttp://www.redalyc.org/html/3236/323631115006/Avances en Ciencias e Ingeniería, vol. 5, núm. 2, abril-junio, 2014, pp. 73-83Barjafidalgo, C., Guimaraes, J.A. & Carlini, C.R. (1991). Lipoxygenase-mediated secretory effect of canatoxin, the toxic protein from canavalia-ensiformisseeds. Toxicon, 29,453-459.Becker-Ritt, A.B., Martinelli, A.H.S., Mitidieri, S., Feder, V., Wassermann, G.E., Santi, L., et al. (2007). Antifungal activity of plant and bacterial ureases. Toxicon, 50,971-983.Bennett, I.J., Mcdavid, D.A.J. & Mccomb, J.A. (2003). The influence of ammonium nitrate, pH and indole butyric acid on root induction and survival in soil of micropropagated Eucalyptus globulus. Biologia Plantarum, 47,355-360.Carlini, C.R. & Grossi-De-Sa, M.F. (2002). Plant toxic proteins with insecticidal properties. A review on their potentialities as bioinsecticides. Toxicon, 40, 1515-1539.Carlini, C.R., Oliveira, A.E.A., Azambuja, P., Xavier, J. & Wells, M.A. (1997). Biological effects of canatoxin in different insect models: Evidence for a proteolytic activation of the toxin by insect cathepsinlike enzymes. Journal of Economic Entomology, 90,340-348.Carlini, C.R. & Polacco, J.C. (2008). Toxic properties of urease. Crop Science, 48, 1665-1672.Chaillou, S., Vessey, J.K., Morotgaudry, J.F., Raper, C.D., Henry, L.T. & Boutin, J.P. (1991). Expression of characteristics of ammonium nutrition as affected by ph of the root medium. Journal of Experimental Botany, 42,189-196.Debergh, P.C. (1983). Effects of agar brand and concentration on the tissue-culture medium. Physiologia Plantarum, 59, 270-276.FAO (2005). Some commonly used cover crop species. Food and Agriculture Organization of the United Nations. http://www.fao.org/ag/ca/2b.html, acceso 30/11/2013. 10. Ferreira-Dasilva, C.T., Gombarovits, M.E.C., Masuda, H., Oliveira, C.M. & Carlini, C.R. (2000). Proteolytic activation of canatoxin, a plant toxic protein, by insect Cathepsin-like enzymes. Archives of Insect Biochemistry and Physiology, 44, 162-171.Follmer, C., Barcellos, G.B.S., Zingali, R.B., Machado, O.L.T., Alves, E.W., Barja-Fidalgo, C., et al. (2001). Canatoxin, a toxic protein from jack beans (Canavalia ensiformis), is a variant form of urease (EC 3.5.1.5): biological effects of urease independent of its ureolytic activity. Biochemical Journal, 360, 217-224.Follmer, C., Real-Guerra, R., Wasserman, G.E., Olivera-Severo, D. & Carlini, C.R. (2004). Jackbean, soybean and Bacillus pasteurii ureases - Biological effects unrelated to ureolytic activity. European Journal of Biochemistry, 271, 1357-1363.Goff, W.L. & Yunker, C.E. (1988). Effects of ph, buffers and medium-storage on the growth of babesia-bovis invitro. International Journal for Parasitology, 18, 775-778.Hwang, D., Kim, S.G. & Kwon, Y.M. (1996). Canavanine synthesis in the in vitro propagated tissues of Canavalia lineata. Plant Cell Reports, 16,180-183.Kovacs, P. (1982). Influence of different fixing agents on lectin binding by the tetrahymena. Acta Histochemica, 71, 245-252.Lang, B. & Kaiser, W.M. (1994). Solute content and energy status of roots of barley plants cultivated at different ph on nitrate-nitrogen or ammonium-nitrogen. New Phytologist, 128,451-459.Leifert, C., Pryce, S., Lumsden, P.J. & Waites, W.M. (1992). Effect of medium acidity on growth and rooting of different plant species growing in vitro. Plant Cell, Tissue and Organ Culture, 30(3), 171-179.Mamiya, G., Takishima, K., Masakuni, M., Kayumi, T. & Ogawa, K. (1987). Complete amino-acid-sequence of jack bean urease. Journal of Protein Chemistry, 6, 55-59.Mamiya, G., Takishima, K., Masakuni, M., Kayumi, T., Ogawa, K. & Sekita, T. (1985). Complete amino-acid sequence of jack bean urease. Proceedings of the Japan Academy Series B-Physical and Biological Sciences, 61,395-398.Mulinari, F., Becker-Ritt, A.B., Demartini, D.R., Ligabue-Braun, R., Staniscuaski, F., Verli, H., et al. (2011). Characterization of JBURE-IIb isoform of Canavalia ensiformis (L.) DC urease. Biochimica Et Biophysica Acta-Proteins and Proteomics, 1814(12), 1758-1768.Mulinari, F., Staniscuaski, F., Bertholdo-Vargas, L.R., Postal, M., Oliveira-Neto, O.B., Riden, D.J., et al. (2007). Jaburetox-2Ec: An insecticidal peptide derived from an isoform of urease from the plant Canavalia ensiformis . Peptides, 28, 2042-2050. 22. Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco cultures. Physioliologia Plantarum, 15, 473-497.Orlikowska, T. (1992). Effects of mineral-composition and acidity of media, saccharose level, brand and quantity of agar on rooting of fruit rootstocks invitro. Biologia Plantarum, 34, 45-52.Pasqua, G., Manes, F., Monacelli, B., Natale, L. & Anselmi, S. (2002). Effects of the culture medium pH and ion uptake in in vitro vegetative organogenesis in thin cell layers of tobacco. Plant Science, 162, 947-955.Piovesan, A.R., Staniscuaski, F., Marco-Salvadori, J., Real-Guerra, R., Defferrari, M.S. & Carlini, C.R. (2008). Stage-specific gut proteinases of the cotton stainer bug Dysdercus peruvianus: Role in the release of entomotoxic peptides from Canavalia ensiformis urease. Insect Biochemistry and Molecular Biology, 38, 1023-1032.Pires-Alves, M., Grossi-De-Sa, M.F., Barcellos, G.B.S., Carlini, C.R. & Moraes, M.G. (2003). Characterization and expression of a novel member (JBURE-II) of the urease gene family from jackbean Canavalia ensiformis (L.) DC. Plant and Cell Physiology, 44, 139-145.Postal, M., Martinelli, A.H.S., Becker-Ritt, A.B., Ligabue-Braun, R., Demartini, D.R., Ribeiro, S.F.F., et al. (2012). Antifungal properties of Canavalia ensiformis urease and derived peptides. Peptides, 38, 22-32.Raven, J.A. & Smith, F.A. (1976). Nitrogen assimilation and transport in vascular land plants in relation to intracellular ph regulation. New Phytologist, 76, 415-431.Ribeirodasilva, G., Carlini, C.R., Piresbarbosa, R. & Guimaraes, J.A. (1986). Blood-glucose alterations induced in rats by canatoxin, a protein isolated from jack bean (canavalia-ensiformis) seeds. Toxicon, 24, 775-782.Ribeirodasilva, G. & Prado, J.F. (1993). Increased insulin circulating levels induced by canatoxin in rats. Toxicon, 31,1131-1136.Riddles, P.W., Whan, V., Blakeley, R.L. & Zerner, B. (1991). Cloning and sequencing of a jack bean urease-encoding cdna. Gene, 108,265-267.Sathyanarayana, B.N. & Blake, J. (1994). The effect of nitrogen sources and initial ph of the media with or without buffer on invitro rooting of jackfruit (artocarpus-heterophyllus lam). Physiology, growth and development of plants in culture Conference entitled "Physiology, growth and development of plants and cells in culture", Physiology, growth and development of plants in culture. Kluwer, pp. 77-82.Sato, A., Barcellos, G.B.S., Riedel, E.C., Carneiro, J.A., Carlini, C.R. & Esquibel, M.A. (1993). The presence of concanavalin-a and canatoxin in canavalia-ensiformisdc tissue-culture. Plant Cell Reports, 12,233-236.Schenk, N., Hsiao, K.C. & Bornman, C.H. (1991). Avoidance of precipitation and carbohydrate breakdown in autoclaved plant-tissue culture media. Plant Cell Reports, 10,115-119.Skirvin, R.M., Mcpheeters, K.D. & Norton, M. (1994). Sources and frequency of somaclonal variation. Hortscience, 29, 1232-1237.Sridhar, K.R. & Seena, S. (2006). Nutritional and antinutritional significance of four unconventional legumes of the genus Canavalia - A comparative study. Food Chemistry, 99, 267-288.Staniscuaski, F., Brugge, V.T., Carlini, C.R. & Orchard, I. (2009). In vitro effect of Canavalia ensiformis urease and the derived peptide Jaburetox-2Ec on Rhodnius prolixus Malpighian tubules. Journal of Insect Physiology, 55 , 255-263.Tan, X.W., Ikeda, H. & Oda, M. (2000). The absorption, translocation, and assimilation of urea, nitrate or ammonium in tomato plants at different plant growth stages in hydroponic culture. Scientia Horticulturae, 84, 275-283.Woodward, A.J., Bennett, I.J. & Pusswonge, S. (2006). The effect of nitrogen source and concentration, medium pH and buffering on in vitro shoot growth and rooting in Eucalyptus marginata. Scientia Horticulturae, 110, 208-213.Zsoldos, F. & Haunold, E. (1982). Influence of 2, 4-d and low ph on potassium, ammonium and nitrate uptake by rice roots. Physiologia Plantarum, 54, 63-68.Avances en Ciencias e IngenieríaCanavalia ensiformisIn vitroCultivopHCanavalia ensiformisIn vitroCulturespHEfecto del pH y el peso inicial de implante sembrado en la multiplicación callogénica de canavalia ensiformisEffect of pH and the initial culture weight on the callogenic multiplication of canavalia ensiformisArticleinfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Saldarriaga, Juan F.Lopez, JuliánLopera, CindyBotero, LilianaSaldarriaga, Juan F.; Universidad de Medellín; Universidad de País VascoLopez, Julián; Universidad de MedellínLopera, Cindy; Universidad de MedellínBotero, Liliana; Universidad de MedellínORIGINALArticulo_.htmlArticulo_.htmltext/html443http://repository.udem.edu.co/bitstream/11407/3425/1/Articulo_.html627ebe8939249ccc027e4286f38a180bMD51THUMBNAILportada.JPGportada.JPGimage/jpeg14118http://repository.udem.edu.co/bitstream/11407/3425/2/portada.JPG03860225b1ff27eeaddeea26c3e5d2b1MD5211407/3425oai:repository.udem.edu.co:11407/34252020-05-27 18:15:23.426Repositorio Institucional Universidad de Medellinrepositorio@udem.edu.co

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