Evaluación de persistencia e infección de nematodos entomopatógenos, steinernema feltiae & heterorhabditis bacteriophora, en menta
Gráficas
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2024
- Institución:
- Universidad de Caldas
- Repositorio:
- Repositorio Institucional U. Caldas
- Idioma:
- eng
spa
- OAI Identifier:
- oai:repositorio.ucaldas.edu.co:ucaldas/19817
- Acceso en línea:
- https://repositorio.ucaldas.edu.co/handle/ucaldas/19817
https://repositorio.ucaldas.edu.co/
- Palabra clave:
- Control biológico
Nematodos entomopatógenos
Galleria mellonella
Persistencia
Biological control
Infectivity
Insecto
- Rights
- openAccess
- License
- http://purl.org/coar/access_right/c_abf2
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Evaluación de persistencia e infección de nematodos entomopatógenos, steinernema feltiae & heterorhabditis bacteriophora, en menta Evaluation of the persistence of entomopathogenic nematodes, Steinernema feltiae and Heterorhabditis bacteriophora, in mint pots |
| title |
Evaluación de persistencia e infección de nematodos entomopatógenos, steinernema feltiae & heterorhabditis bacteriophora, en menta |
| spellingShingle |
Evaluación de persistencia e infección de nematodos entomopatógenos, steinernema feltiae & heterorhabditis bacteriophora, en menta Control biológico Nematodos entomopatógenos Galleria mellonella Persistencia Biological control Infectivity Insecto |
| title_short |
Evaluación de persistencia e infección de nematodos entomopatógenos, steinernema feltiae & heterorhabditis bacteriophora, en menta |
| title_full |
Evaluación de persistencia e infección de nematodos entomopatógenos, steinernema feltiae & heterorhabditis bacteriophora, en menta |
| title_fullStr |
Evaluación de persistencia e infección de nematodos entomopatógenos, steinernema feltiae & heterorhabditis bacteriophora, en menta |
| title_full_unstemmed |
Evaluación de persistencia e infección de nematodos entomopatógenos, steinernema feltiae & heterorhabditis bacteriophora, en menta |
| title_sort |
Evaluación de persistencia e infección de nematodos entomopatógenos, steinernema feltiae & heterorhabditis bacteriophora, en menta |
| dc.contributor.none.fl_str_mv |
Long, Elizabeth Yim Zamorano-Montañez, Carolina GIPPA: Producción Agropecuaria (Categoría A1) |
| dc.subject.none.fl_str_mv |
Control biológico Nematodos entomopatógenos Galleria mellonella Persistencia Biological control Infectivity Insecto |
| topic |
Control biológico Nematodos entomopatógenos Galleria mellonella Persistencia Biological control Infectivity Insecto |
| description |
Gráficas |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024-03-06T17:03:08Z 2024-03-06T17:03:08Z 2024-03-06 |
| dc.type.none.fl_str_mv |
Trabajo de grado - Pregrado http://purl.org/coar/resource_type/c_7a1f Text info:eu-repo/semantics/bachelorThesis |
| dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.identifier.none.fl_str_mv |
https://repositorio.ucaldas.edu.co/handle/ucaldas/19817 Universidad de Caldas Repositorio Institucional Universidad de Caldas https://repositorio.ucaldas.edu.co/ |
| url |
https://repositorio.ucaldas.edu.co/handle/ucaldas/19817 https://repositorio.ucaldas.edu.co/ |
| identifier_str_mv |
Universidad de Caldas Repositorio Institucional Universidad de Caldas |
| dc.language.none.fl_str_mv |
eng spa |
| language |
eng spa |
| dc.relation.none.fl_str_mv |
Bal, H. K., Michael, A., & Grewal, P. S. 2014. Genetic selection of the ambush foraging entomopathogenic nematode Steinernema carpocapsae for enhanced dispersal and its associated trade–offs. Evolutionary Ecology, 28, 923–939. Bal, H. K., Taylor, R. A. J., & Grewal, P. S. 2014. Ambush foraging entomopathogenic nematodes employ sprinting emigrants for long distance dispersal in the absence of hosts. Parasitology, 100, 422–432. Boemare NE. 2002. Biology, taxonomy, and systematics of Photorhabdus and Xenorhabdus. In: Gaugler R (ed) Entomopathogenic nematology. CABI Publishing, Wallingford, UK, 35–56. Brown, V. K., Gange, A. C. 1990. Insect herbivory below ground. Advances in Ecological Research, 20, 1–58. Campbell, J. F., Orza, G., Yoder, F., Lewis, E., & Gaugler, R. 1998. Spatial and temporal distribution of endemic and released entomopathogenic nematode populations in turfgrass. Entomologia Experimentalis et Applicata, 86, 1–11. Fierer, N., Strickland, M. S., Liptzin, D., Bradford, M. A., & Cleveland, C. C. 2009. Global patterns in belowground communities. Ecology Letters, 12, 1–12. Forst S, Clarke D. 2002. Bacteria-nematode symbiosis. In: Gaugler R (ed) Entomopathogenic nematology. CABI Publishing, Wallingford, UK, 57–77. Glaser RW, Farrell CC. 1935. Field experiments with the Japanese beetle and its nematode parasite. J N Y Entomol Soc. 43. Glazer I. 2015. Improvement of entomopathogenic nematodes: A genetic approach. In: R. Campos-Herrera, editor. Nematode Pathogenesis of Insects and Other Pests: Ecology and Applied Technologies for Sustainable Plant and Crop Protection. Springer International Publishing, 29–55. Gumz, M.S.P. 2007. Development of Remote Sensing Based Site Specific Weed Management for Midwest Mint Production, Doctor of Philoshophy Purdue University. Hawley IM, Hallock HC. 1936. Life history and control of the Asiatic Garden beetle. USDA Circular 246: 1-20. [Revision of 1932 issue by H.C. Hallock of same title.] Kaya HK, Koppenhofer AM. 1999. Biology and ecology of insecticidal nematodes. In: Optimal use of insecticidal nematodes in pest management. IntechOpen, Croatia, 1–8. Kaya, H. K., & Stock, P. 1997. Techniques in insect nematology. In L. A. Lacey (Ed.), Manual of techniques in insect pathology. San Diego, CA: Academic. 281–324. Kelley, Paul E. 2013. The Asiatic Garden Beetle Maladera Castanea (Arrow 1913) (Coleoptera: Scarabaeidae): EENY554/IN992, 4/2013”. EDIS 2013 (6). https://doi.org/10.32473/edis-in992- 2013. Poinar, G. O. 1984. The natural history of nematodes. Prentice-Hall, Englewood Cliffs, NJ. USDA-NASS. 2021. U.S. Department of Agriculture–National Agriculture Statistics Service Quick stats. https://quickstats.nass.usda.gov/ results/D9C05018-B9D8-3906-B630- 9C077F73547B. Accessed: December 13, 2023 Shields, Elson J., Testa, Antonio M. 2020. "Multi-year Biological Control of Black Vine Weevil, Otiorhynchus sulcatus, with Persistent Entomopathogenic Nematodes," The Great Lakes Entomologist, vol 53 (2). Shields, E., Testa, A., Rusinek, T., Bornt, C. 2021. Management of Wireworms in Sweet Potatoes with Persistent NY Entomopathogenic Nematodes. The Great Lakes Entomologist, vol 54 (2). Skowronek, M., Sajnaga, E., Pleszczy ´nska, M., Kazimierczak, W., Lis, M., Wiater, A. 2020. Bacteria from the midgut of common cockchafer (Melolontha melolontha L.) larvae exhibiting antagonistic activity against bacterial symbionts of entomopathogenic nematodes: Isolation and molecular identification. Int. J. Mol. Sci. 21, 580. Testa, A. M., J. Shields. 2017. Low Labor “in vivo” Mass Rearing Method for Entomopathogenic Nematodes. Biocontrol 106: 77–82. Wilson, M. J., Lewis, E. E., Yoder, F., & Gaugler, R. 2003. Application pattern and persistence of the entomopathogenic nematode Heterorhabditis bacteriophora. Biological Control, 26, 180– 188. White, G. F. 1927. A method for obtaining infective nematode larvae from cultures. Science. 66: 302–303. Yan, X., Shahid Arain, M., Lin, Y., Gu, X., Zhang, L., Li, J., Han, R. 2020. Efficacy of entomopathogenic nematodes against the tobacco cutworm, Spodoptera litura (Lepidoptera: Noctuidae). J. Econ. Entomol. 113, 64–72. |
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info:eu-repo/semantics/openAccess info:eu-repo/semantics/openAccess info:eu-repo/semantics/openAccess info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 |
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Facultad de Ciencias Agropecuarias Manizales Ingeniería Agronómica |
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Facultad de Ciencias Agropecuarias Manizales Ingeniería Agronómica |
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Universidad de Caldas |
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Evaluación de persistencia e infección de nematodos entomopatógenos, steinernema feltiae & heterorhabditis bacteriophora, en mentaEvaluation of the persistence of entomopathogenic nematodes, Steinernema feltiae and Heterorhabditis bacteriophora, in mint potsControl biológicoNematodos entomopatógenosGalleria mellonellaPersistenciaBiological controlInfectivityInsectoGráficasLos nematodos entomopatógenos se aplican como agentes de control biológico de plagas de insectos que habitan en el suelo, como los gusanos blancos o ciertas larvas, en diversos sistemas agrícolas y pueden ser eficaces solos o como complemento de los insecticidas aplicados al suelo. Sin embargo, no está claro cuánto tiempo estos agentes de control biológico pueden persistir en el suelo y cómo su eficacia contra las plagas de insectos que habitan en el suelo cambia a lo largo del tiempo. El objetivo de este experimento es evaluar la supervivencia (persistencia) y la infectividad de dos especies de nematodos entomopatógenos, Steinernema feltiae (Sf), y Heterorhabditis bacteriophora (Hb), solos y en combinación en macetas de menta durante un periodo de dos semanas, recogiendo muestras de suelo y cebándolas con gusanos de la cera bajo condiciones de laboratorio. El tratamiento que consistió únicamente en S. feltiae mostró la mejor persistencia e infección de las larvas de cera a lo largo del experimento, teniendo un buen comportamiento en la recuperación de la población tras la adaptación a las condiciones del medio de la maceta, y teniendo un incremento en la infección hasta alcanzar un porcentaje de infección estable al final del experimento, siendo este un comportamiento muy diferente al de H. bacteriophora que no estuvo presente al final del experimento en ninguno de los dos tratamientos en los que se aplicó.Entomopathogenic nematodes are applied as biological control agents of soil-dwelling insect pests, like white grubs or caterpillars, in a variety of agricultural systems and can be effective alone or as a complement to soil-applied insecticides. However, it is unclear how long these biological control agents may persist in the soil and how their efficacy against soil-dwelling insect pests changes over time. The goal of this experiment was to evaluate the survival (persistence) and infectivity of two species of entomopathogenic nematodes, Steinernema feltiae (Sf), and Heterorhabditis bacteriophora (Hb), alone or in combination in mint pots over a two-week period by collecting soil samples and baiting them with waxworms under laboratory conditions. The treatment that consisted only of S. feltiae showed the best persistence over 14 days. With regards to infection of the waxworm larvae by each entomopathogenic nematode species throughout the experiment, we found a significant time by species interaction, such that H. bacteriophora showed the highest percent infection up to 7 days after treatment, while S. feltiae showed the highest percent infection 14 days after treatment.1. Objetivos / 1.1. Objetivo general / 1.2. Objetivos específicos / 2. Introducción / 2.1. Sistema aplicado / 3. Materiales y métodos / 4. Análisis estadístico / 5. Resultados / 6. Discusión / 7. Conclusión / 8. Agradecimientos / 9. Referencias bibliográficasUniversitarioIngeniero(a) Agronómico(a)Control BiológicoFacultad de Ciencias AgropecuariasManizalesIngeniería AgronómicaLong, Elizabeth YimZamorano-Montañez, CarolinaGIPPA: Producción Agropecuaria (Categoría A1)Cabrera Castellanos, Cristian Camilo2024-03-06T17:03:08Z2024-03-06T17:03:08Z2024-03-06Trabajo de grado - Pregradohttp://purl.org/coar/resource_type/c_7a1fTextinfo:eu-repo/semantics/bachelorThesishttp://purl.org/coar/version/c_970fb48d4fbd8a85application/pdfapplication/pdfapplication/pdfapplication/pdfhttps://repositorio.ucaldas.edu.co/handle/ucaldas/19817Universidad de CaldasRepositorio Institucional Universidad de Caldashttps://repositorio.ucaldas.edu.co/engspaBal, H. K., Michael, A., & Grewal, P. S. 2014. Genetic selection of the ambush foraging entomopathogenic nematode Steinernema carpocapsae for enhanced dispersal and its associated trade–offs. Evolutionary Ecology, 28, 923–939.Bal, H. K., Taylor, R. A. J., & Grewal, P. S. 2014. Ambush foraging entomopathogenic nematodes employ sprinting emigrants for long distance dispersal in the absence of hosts. Parasitology, 100, 422–432.Boemare NE. 2002. Biology, taxonomy, and systematics of Photorhabdus and Xenorhabdus. In: Gaugler R (ed) Entomopathogenic nematology. CABI Publishing, Wallingford, UK, 35–56.Brown, V. K., Gange, A. C. 1990. Insect herbivory below ground. Advances in Ecological Research, 20, 1–58.Campbell, J. F., Orza, G., Yoder, F., Lewis, E., & Gaugler, R. 1998. Spatial and temporal distribution of endemic and released entomopathogenic nematode populations in turfgrass. Entomologia Experimentalis et Applicata, 86, 1–11.Fierer, N., Strickland, M. S., Liptzin, D., Bradford, M. A., & Cleveland, C. C. 2009. Global patterns in belowground communities. Ecology Letters, 12, 1–12.Forst S, Clarke D. 2002. Bacteria-nematode symbiosis. In: Gaugler R (ed) Entomopathogenic nematology. CABI Publishing, Wallingford, UK, 57–77.Glaser RW, Farrell CC. 1935. Field experiments with the Japanese beetle and its nematode parasite. J N Y Entomol Soc. 43.Glazer I. 2015. Improvement of entomopathogenic nematodes: A genetic approach. In: R. Campos-Herrera, editor. Nematode Pathogenesis of Insects and Other Pests: Ecology and Applied Technologies for Sustainable Plant and Crop Protection. Springer International Publishing, 29–55.Gumz, M.S.P. 2007. Development of Remote Sensing Based Site Specific Weed Management for Midwest Mint Production, Doctor of Philoshophy Purdue University.Hawley IM, Hallock HC. 1936. Life history and control of the Asiatic Garden beetle. USDA Circular 246: 1-20. [Revision of 1932 issue by H.C. Hallock of same title.]Kaya HK, Koppenhofer AM. 1999. Biology and ecology of insecticidal nematodes. In: Optimal use of insecticidal nematodes in pest management. IntechOpen, Croatia, 1–8.Kaya, H. K., & Stock, P. 1997. Techniques in insect nematology. In L. A. Lacey (Ed.), Manual of techniques in insect pathology. San Diego, CA: Academic. 281–324.Kelley, Paul E. 2013. The Asiatic Garden Beetle Maladera Castanea (Arrow 1913) (Coleoptera: Scarabaeidae): EENY554/IN992, 4/2013”. EDIS 2013 (6). https://doi.org/10.32473/edis-in992- 2013.Poinar, G. O. 1984. The natural history of nematodes. Prentice-Hall, Englewood Cliffs, NJ.USDA-NASS. 2021. U.S. Department of Agriculture–National Agriculture Statistics Service Quick stats. https://quickstats.nass.usda.gov/ results/D9C05018-B9D8-3906-B630- 9C077F73547B. Accessed: December 13, 2023Shields, Elson J., Testa, Antonio M. 2020. "Multi-year Biological Control of Black Vine Weevil, Otiorhynchus sulcatus, with Persistent Entomopathogenic Nematodes," The Great Lakes Entomologist, vol 53 (2).Shields, E., Testa, A., Rusinek, T., Bornt, C. 2021. Management of Wireworms in Sweet Potatoes with Persistent NY Entomopathogenic Nematodes. The Great Lakes Entomologist, vol 54 (2).Skowronek, M., Sajnaga, E., Pleszczy ´nska, M., Kazimierczak, W., Lis, M., Wiater, A. 2020. Bacteria from the midgut of common cockchafer (Melolontha melolontha L.) larvae exhibiting antagonistic activity against bacterial symbionts of entomopathogenic nematodes: Isolation and molecular identification. Int. J. Mol. Sci. 21, 580.Testa, A. M., J. Shields. 2017. Low Labor “in vivo” Mass Rearing Method for Entomopathogenic Nematodes. Biocontrol 106: 77–82.Wilson, M. J., Lewis, E. E., Yoder, F., & Gaugler, R. 2003. Application pattern and persistence of the entomopathogenic nematode Heterorhabditis bacteriophora. Biological Control, 26, 180– 188.White, G. F. 1927. A method for obtaining infective nematode larvae from cultures. Science. 66: 302–303.Yan, X., Shahid Arain, M., Lin, Y., Gu, X., Zhang, L., Li, J., Han, R. 2020. Efficacy of entomopathogenic nematodes against the tobacco cutworm, Spodoptera litura (Lepidoptera: Noctuidae). J. Econ. Entomol. 113, 64–72.info:eu-repo/semantics/openAccessinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2oai:repositorio.ucaldas.edu.co:ucaldas/198172024-07-16T21:39:57Z |