Thermal, structural and mechanical characterization of Nephila clavipes spider silk in southwest Colombia

Some physical properties of spider silks, including mechanical strength and toughness, have been studied in many laboratories worldwide. Given that this silk is organic in nature, composed of protein, and has similar properties to metal wires or polymers, it has the potential for application in medi...

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Autores:: Aparicio Rojas, Gladis Miriam
Diaz-Puentes, Edgar
Medina Vargas, Giovanni
Aparicio Rojas, Gladis Miriam

Tipo de recurso:: Article of journal

Fecha de publicación:: 2020

Institución:: Universidad Autónoma de Occidente

Repositorio:: RED: Repositorio Educativo Digital UAO

Idioma:: eng

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dc.title.eng.fl_str_mv	Thermal, structural and mechanical characterization of Nephila clavipes spider silk in southwest Colombia
title	Thermal, structural and mechanical characterization of Nephila clavipes spider silk in southwest Colombia
spellingShingle	Thermal, structural and mechanical characterization of Nephila clavipes spider silk in southwest Colombia Ciencia de los materiales Ingeniería mecánica Termodinámica Fibras animales Animal fibers Materials science Mechanical engineering Thermodynamics Nanomaterials Materials application Materials characterization Materials physics Materials mechanics Materials property Environmental analysis Spider silk Nephila clavipes Mechanical characterization Structural characterization and thermal behavior
title_short	Thermal, structural and mechanical characterization of Nephila clavipes spider silk in southwest Colombia
title_full	Thermal, structural and mechanical characterization of Nephila clavipes spider silk in southwest Colombia
title_fullStr	Thermal, structural and mechanical characterization of Nephila clavipes spider silk in southwest Colombia
title_full_unstemmed	Thermal, structural and mechanical characterization of Nephila clavipes spider silk in southwest Colombia
title_sort	Thermal, structural and mechanical characterization of Nephila clavipes spider silk in southwest Colombia
dc.creator.fl_str_mv	Aparicio Rojas, Gladis Miriam Diaz-Puentes, Edgar Medina Vargas, Giovanni Aparicio Rojas, Gladis Miriam
dc.contributor.author.spa.fl_str_mv	Aparicio Rojas, Gladis Miriam Diaz-Puentes, Edgar
dc.contributor.author.none.fl_str_mv	Medina Vargas, Giovanni Aparicio Rojas, Gladis Miriam
dc.contributor.corporatename.eng.fl_str_mv	Elsevier Ltd
dc.subject.armarc.spa.fl_str_mv	Ciencia de los materiales Ingeniería mecánica Termodinámica Fibras animales
topic	Ciencia de los materiales Ingeniería mecánica Termodinámica Fibras animales Animal fibers Materials science Mechanical engineering Thermodynamics Nanomaterials Materials application Materials characterization Materials physics Materials mechanics Materials property Environmental analysis Spider silk Nephila clavipes Mechanical characterization Structural characterization and thermal behavior
dc.subject.armarc.eng.fl_str_mv	Animal fibers
dc.subject.proposal.eng.fl_str_mv	Materials science Mechanical engineering Thermodynamics Nanomaterials Materials application Materials characterization Materials physics Materials mechanics Materials property Environmental analysis Spider silk Nephila clavipes Mechanical characterization Structural characterization and thermal behavior
description	Some physical properties of spider silks, including mechanical strength and toughness, have been studied in many laboratories worldwide. Given that this silk is organic in nature, composed of protein, and has similar properties to metal wires or polymers, it has the potential for application in medicine, nanoelectronics, and other related areas. In this study, we worked on spider silk from the Nephila clavipes species collected from the wild and kept it in the nursery of the Autonomous University of the West, Cali, Colombia, to determine its physical, thermal, and mechanical properties, seeking possible applications in the medical and industrial sectors and comparing the material properties of the silk from the species from southwestern Colombia with those of the previously studied species from other regions. The mechanical characterization of the material was performed using a universal testing machine; thermal behavior was captured by a thermogravimetric analysis, differential scanning calorimetry, and mass spectrometry; and structural characterization was performed using diffraction X-rays. The results of the thermal characterization demonstrate that the spider silk loses 10 % of water content at 150 °C with significant changes at 400 °C, while the mechanical characterization indicates that the spider silk is much tougher than Kevlar 49 and Nylon 6 since it is capable of absorbing more energy before rupture
publishDate	2020
dc.date.issued.none.fl_str_mv	2020-10-12
dc.date.accessioned.none.fl_str_mv	2022-01-19T15:58:45Z
dc.date.available.none.fl_str_mv	2022-01-19T15:58:45Z
dc.type.spa.fl_str_mv	Artículo de revista
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dc.relation.citationedition.spa.fl_str_mv	Volumen 6, número 11 (2020)
dc.relation.citationendpage.spa.fl_str_mv	6
dc.relation.citationissue.spa.fl_str_mv	11
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dc.relation.citationvolume.spa.fl_str_mv	6
dc.relation.cites.eng.fl_str_mv	Aparicio Rojas, G. M., Medina Vargas, G., Díaz Puentes, E. (2020). Thermal, structural and mechanical characterization of Nephila clavipes spider silk in southwest Colombia. Heliyon. (Vol. 6 (11), pp. 1-6. https://doi.org/10.1016/j.heliyon.2020.e05262
dc.relation.ispartofjournal.eng.fl_str_mv	Heliyon
dc.relation.references.none.fl_str_mv	Alencastre, J., Olarte, C., Rivera, R., Mu~noz, J., 2016. Estudio Din amico del Sistema Ara~na-Tela de Ara~na en condiciones de Resonancia. Inf. Tecnol. 27 (4), 139–144. Alencatre, J., 2015. Caracterizaci on de las propiedades din amicas de la seda de ara~na (Tesis doctoral). Universidad polit ecnica de Madrid, Espa~na. Bartoleti, F., Peres, E., Fuentes, F., Da silva, M., Solferini, V., 2018. Phylogeography of the widespread spider Nephila clavipes (Araneae: Araneidae) in South America indicates geologically and climatically driven lineage diversification. J. Biogeogr. 45 (6), 1246–1260. Borja, A., Gonzales, I., Eceiza, A., 2015. Hacia la mímesis de la seda de ara~na a partir de poliuretanos con segmentos cortos de unidades rígidas y semiflexibles. Revista lationoamericana de metalurgia y materiales 35 (1), 39–48. Bowen, C., Dai, B., Sargent, C., Bai, W., Ladiwala, P., Feng, H., Huang, W., Kaplan, D., Galazka, J., Zhang, F., 2018. Recombinant spidroins fully replicate primary mechanical properties of natural spider silk. Biomacromolecules 19 (9), 3853–3860. Cunniff, P.M., Fossey, S.A., Auerbach, M.A., Song, J.W., Kaplan, D.L., Adams, W.W., Eby, R.K., Mahoney, D., Vezie, D.L., 1994. Mechanical and thermal properties of dragline silk from the spider Nephila clavipes. Polym. Adv. Technol. 5 (8), 401–410. Dos santos-Pint, J., Arcuri, A., Esteves, F., Palma, S., Lubec, G., 2018. Spider silk proteome provides insight into the structural characterization of Nephila Clavipes flagelliform spidroin. Sci. Rep. 8 (1), 14674. Du, N., Liu, X.Y., Narayanan, J., Li, L., Lim, M.M., Li, D., 2006. Design of superior spider silk: from nanostructure to mechanical properties. Biophys. J. 91 (12), 4528–4535. Gomes, S., Gallego-Llamas, J., Leonor, I.B., Mano, J.F., Reis, R.L., Kaplan, D.L., 2011. Biological responses to spider silk–antibiotic fusion protein. J. Tissue Eng. Regen. Med. 6 (5), 356–368. Hauptmann, V., Weichert, N., Rakhimova, M., Conrad, U., 2013. Spider silks from plantsa challenge to create native-sized spidroins. Biotechnol. J. 8, 1183–1192. Hinman, M.B., Jones, J.A., Lewis, R.V., 2000. Synthetic spider silk: a modular fiber. Trends Biotechnol. 18 (9), 374–379. Perea, G.B., P erez-Rigueiro, J., Plaza, G.R., Guinea, G.V., Elices, M., 2010. Efecto de la longitud de onda de la radiaci on UV sobre la seda de ara~na. Anales de Mec anica de la Fractura 27, 41–43. Saravanan, D., 2006. Spider silk-structure, properties and spinning. J. Text. Appar. Technol. Manag. 5 (1), 1–20. Simmons, A.H., Michal, C.A., Jelenski, L.W., 1996. Molecular orientation and twocomponent nature of the crystalline fraction of spider dragline silk. Science 271 (5245), 84–87. Soler, A., 2013. An alisis de la topología de la tela de ara~na en su comportamiento frente a impacto. (Tesis de pregrado). Universidad Carlos III de Madrid. Legan es, Espa~na. Swanson, B.O., Blackledge, T.A., Beltr an, J., Hayashi, C.Y., 2006. Variation in the material properties of spider dragline silk across species. Appl. Phys. A 82, 213–218. Vollrath, F., 2000. Strength and structure of spiders’ silks. Rev. Mol. Biotechnol. 74 (2), 67–83. Xu, M., Lewis, R.V., 1990. Structure of a protein superfiber: spider dragline silk. Proc. Natl. Acad. Sci. U.S.A. 87 (18), 7120–7124
dc.rights.spa.fl_str_mv	Derechos reservados Revista Heliyon
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spelling	Aparicio Rojas, Gladis Miriam5cfba808641898ab3187ee7be4f6ec47Diaz-Puentes, Edgar8e2531064a76fb6b02ca83d4831d83c1Medina Vargas, Giovannivirtual::3329-1Aparicio Rojas, Gladis Miriamvirtual::303-1Elsevier Ltd2022-01-19T15:58:45Z2022-01-19T15:58:45Z2020-10-12https://hdl.handle.net/10614/1355224058440Some physical properties of spider silks, including mechanical strength and toughness, have been studied in many laboratories worldwide. Given that this silk is organic in nature, composed of protein, and has similar properties to metal wires or polymers, it has the potential for application in medicine, nanoelectronics, and other related areas. In this study, we worked on spider silk from the Nephila clavipes species collected from the wild and kept it in the nursery of the Autonomous University of the West, Cali, Colombia, to determine its physical, thermal, and mechanical properties, seeking possible applications in the medical and industrial sectors and comparing the material properties of the silk from the species from southwestern Colombia with those of the previously studied species from other regions. The mechanical characterization of the material was performed using a universal testing machine; thermal behavior was captured by a thermogravimetric analysis, differential scanning calorimetry, and mass spectrometry; and structural characterization was performed using diffraction X-rays. The results of the thermal characterization demonstrate that the spider silk loses 10 % of water content at 150 °C with significant changes at 400 °C, while the mechanical characterization indicates that the spider silk is much tougher than Kevlar 49 and Nylon 6 since it is capable of absorbing more energy before rupture6 páginasapplication/pdfengElsevier LtdUnited KingdomDerechos reservados Revista Heliyonhttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)http://purl.org/coar/access_right/c_abf2https://www.sciencedirect.com/science/article/pii/S2405844020321058Thermal, structural and mechanical characterization of Nephila clavipes spider silk in southwest ColombiaArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Ciencia de los materialesIngeniería mecánicaTermodinámicaFibras animalesAnimal fibersMaterials scienceMechanical engineering ThermodynamicsNanomaterialsMaterials applicationMaterials characterizationMaterials physicsMaterials mechanicsMaterials propertyEnvironmental analysisSpider silkNephila clavipesMechanical characterizationStructural characterization and thermal behaviorVolumen 6, número 11 (2020)61116Aparicio Rojas, G. M., Medina Vargas, G., Díaz Puentes, E. (2020). Thermal, structural and mechanical characterization of Nephila clavipes spider silk in southwest Colombia. Heliyon. (Vol. 6 (11), pp. 1-6. https://doi.org/10.1016/j.heliyon.2020.e05262HeliyonAlencastre, J., Olarte, C., Rivera, R., Mu~noz, J., 2016. Estudio Din amico del Sistema Ara~na-Tela de Ara~na en condiciones de Resonancia. Inf. Tecnol. 27 (4), 139–144.Alencatre, J., 2015. Caracterizaci on de las propiedades din amicas de la seda de ara~na (Tesis doctoral). Universidad polit ecnica de Madrid, Espa~na.Bartoleti, F., Peres, E., Fuentes, F., Da silva, M., Solferini, V., 2018. Phylogeography of the widespread spider Nephila clavipes (Araneae: Araneidae) in South America indicates geologically and climatically driven lineage diversification. J. Biogeogr. 45 (6), 1246–1260.Borja, A., Gonzales, I., Eceiza, A., 2015. Hacia la mímesis de la seda de ara~na a partir de poliuretanos con segmentos cortos de unidades rígidas y semiflexibles. Revista lationoamericana de metalurgia y materiales 35 (1), 39–48.Bowen, C., Dai, B., Sargent, C., Bai, W., Ladiwala, P., Feng, H., Huang, W., Kaplan, D., Galazka, J., Zhang, F., 2018. Recombinant spidroins fully replicate primary mechanical properties of natural spider silk. Biomacromolecules 19 (9), 3853–3860.Cunniff, P.M., Fossey, S.A., Auerbach, M.A., Song, J.W., Kaplan, D.L., Adams, W.W., Eby, R.K., Mahoney, D., Vezie, D.L., 1994. Mechanical and thermal properties of dragline silk from the spider Nephila clavipes. Polym. Adv. Technol. 5 (8), 401–410.Dos santos-Pint, J., Arcuri, A., Esteves, F., Palma, S., Lubec, G., 2018. Spider silk proteome provides insight into the structural characterization of Nephila Clavipes flagelliform spidroin. Sci. Rep. 8 (1), 14674.Du, N., Liu, X.Y., Narayanan, J., Li, L., Lim, M.M., Li, D., 2006. Design of superior spider silk: from nanostructure to mechanical properties. Biophys. J. 91 (12), 4528–4535.Gomes, S., Gallego-Llamas, J., Leonor, I.B., Mano, J.F., Reis, R.L., Kaplan, D.L., 2011. Biological responses to spider silk–antibiotic fusion protein. J. Tissue Eng. Regen. Med. 6 (5), 356–368.Hauptmann, V., Weichert, N., Rakhimova, M., Conrad, U., 2013. Spider silks from plantsa challenge to create native-sized spidroins. Biotechnol. J. 8, 1183–1192.Hinman, M.B., Jones, J.A., Lewis, R.V., 2000. Synthetic spider silk: a modular fiber. Trends Biotechnol. 18 (9), 374–379.Perea, G.B., P erez-Rigueiro, J., Plaza, G.R., Guinea, G.V., Elices, M., 2010. Efecto de la longitud de onda de la radiaci on UV sobre la seda de ara~na. Anales de Mec anica de la Fractura 27, 41–43.Saravanan, D., 2006. Spider silk-structure, properties and spinning. J. Text. Appar. Technol. Manag. 5 (1), 1–20.Simmons, A.H., Michal, C.A., Jelenski, L.W., 1996. Molecular orientation and twocomponent nature of the crystalline fraction of spider dragline silk. Science 271 (5245), 84–87.Soler, A., 2013. An alisis de la topología de la tela de ara~na en su comportamiento frente a impacto. (Tesis de pregrado). Universidad Carlos III de Madrid. Legan es, Espa~na.Swanson, B.O., Blackledge, T.A., Beltr an, J., Hayashi, C.Y., 2006. Variation in the material properties of spider dragline silk across species. Appl. Phys. A 82, 213–218.Vollrath, F., 2000. Strength and structure of spiders’ silks. Rev. Mol. Biotechnol. 74 (2), 67–83.Xu, M., Lewis, R.V., 1990. Structure of a protein superfiber: spider dragline silk. Proc. Natl. Acad. Sci. U.S.A. 87 (18), 7120–7124Comunidad en generalPublicationa6821d1e-14cb-4406-aa9b-07ce284a1868virtual::3329-1b4461b68-2d8c-4ca0-b6fe-cd2e043a2c53virtual::303-1b4461b68-2d8c-4ca0-b6fe-cd2e043a2c53virtual::303-1a6821d1e-14cb-4406-aa9b-07ce284a1868virtual::3329-1https://scholar.google.com/citations?view_op=list_works&hl=es&user=KvMAsMEAAAAJ&authuser=1virtual::3329-1https://scholar.google.com/citations?user=WtTqM8IAAAAJ&hl=esvirtual::303-10000-0002-0731-012Xvirtual::3329-10000-0002-7158-1223virtual::303-1https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000031518virtual::3329-1https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000112399virtual::303-1LICENSElicense.txtlicense.txttext/plain; charset=utf-81665https://red.uao.edu.co/bitstreams/33765d97-1be8-496f-953d-26dda2cefcc1/download20b5ba22b1117f71589c7318baa2c560MD52ORIGINALThermal, structural and mechanical characterization of Nephila clavipes spider silk in southwest Colombia.pdfThermal, structural and mechanical characterization of Nephila clavipes spider silk in southwest Colombia.pdfTexto archivo completo del artículo de revista, PDFapplication/pdf408154https://red.uao.edu.co/bitstreams/11f2e966-23a2-49c4-b90b-61870c86b583/downloadc16b0fdf2af393d1071def9a6ac6eb1bMD53TEXTThermal, structural and mechanical characterization of Nephila clavipes spider silk in southwest Colombia.pdf.txtThermal, structural and mechanical characterization of Nephila clavipes spider silk in southwest Colombia.pdf.txtExtracted texttext/plain27277https://red.uao.edu.co/bitstreams/d1a0c691-13b3-4ff3-8836-a88ba7baaa74/download7305d9c9d673ba7d5bfb47ea36016d8cMD54THUMBNAILThermal, structural and mechanical characterization of Nephila clavipes spider silk in southwest Colombia.pdf.jpgThermal, structural and mechanical characterization of Nephila clavipes spider silk in southwest Colombia.pdf.jpgGenerated Thumbnailimage/jpeg14577https://red.uao.edu.co/bitstreams/024c8d72-963c-46f9-8cfc-8fd5f9a44ef7/download48cdd8b9b77f62d2f1f9673d4a226b0bMD5510614/13552oai:red.uao.edu.co:10614/135522024-03-11 08:41:40.119https://creativecommons.org/licenses/by-nc-nd/4.0/Derechos reservados Revista Heliyonopen.accesshttps://red.uao.edu.coRepositorio Digital Universidad Autonoma de Occidenterepositorio@uao.edu.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

Thermal, structural and mechanical characterization of Nephila clavipes spider silk in southwest Colombia

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