Evaluation of different methods for efficient extraction of hemicelluloses leaves and tops of sugarcane
En Colombia se producen alrededor de 9 millones de toneladas de residuos de la cosecha de la caña de azúcar por hectárea cada año, que no han sido valorizados hacia ningún tipo de producto. Es necesario conocer su composición, en especial el contenido de hemicelulosas, para dilucidar el proceso para...
- Autores:
-
Flórez Pardo, Luz Marina
López Galán, Jorge Enrique
González-Córdoba, Andrea
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2018
- Institución:
- Universidad Autónoma de Occidente
- Repositorio:
- RED: Repositorio Educativo Digital UAO
- Idioma:
- eng
- OAI Identifier:
- oai:red.uao.edu.co:10614/11434
- Acceso en línea:
- http://hdl.handle.net/10614/11434
- Palabra clave:
- Cultivos energéticos
Energy crops
Bioetanol
Residuo lignocelulósico
Polisacáridos
Bioethanol
Lignocellulosic residue
Polysaccharides
- Rights
- openAccess
- License
- Derechos Reservados - Universidad Autónoma de Occidente
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Evaluation of different methods for efficient extraction of hemicelluloses leaves and tops of sugarcane |
| dc.title.alternative.spa.fl_str_mv |
Evaluación de diferentes métodos para la extracción eficiente de hemicelulosas de las hojas y cogollos de la caña de azúcar |
| title |
Evaluation of different methods for efficient extraction of hemicelluloses leaves and tops of sugarcane |
| spellingShingle |
Evaluation of different methods for efficient extraction of hemicelluloses leaves and tops of sugarcane Cultivos energéticos Energy crops Bioetanol Residuo lignocelulósico Polisacáridos Bioethanol Lignocellulosic residue Polysaccharides |
| title_short |
Evaluation of different methods for efficient extraction of hemicelluloses leaves and tops of sugarcane |
| title_full |
Evaluation of different methods for efficient extraction of hemicelluloses leaves and tops of sugarcane |
| title_fullStr |
Evaluation of different methods for efficient extraction of hemicelluloses leaves and tops of sugarcane |
| title_full_unstemmed |
Evaluation of different methods for efficient extraction of hemicelluloses leaves and tops of sugarcane |
| title_sort |
Evaluation of different methods for efficient extraction of hemicelluloses leaves and tops of sugarcane |
| dc.creator.fl_str_mv |
Flórez Pardo, Luz Marina López Galán, Jorge Enrique González-Córdoba, Andrea |
| dc.contributor.author.none.fl_str_mv |
Flórez Pardo, Luz Marina López Galán, Jorge Enrique González-Córdoba, Andrea |
| dc.subject.armarc.spa.fl_str_mv |
Cultivos energéticos |
| topic |
Cultivos energéticos Energy crops Bioetanol Residuo lignocelulósico Polisacáridos Bioethanol Lignocellulosic residue Polysaccharides |
| dc.subject.armarc.eng.fl_str_mv |
Energy crops |
| dc.subject.proposal.spa.fl_str_mv |
Bioetanol Residuo lignocelulósico Polisacáridos |
| dc.subject.proposal.eng.fl_str_mv |
Bioethanol Lignocellulosic residue Polysaccharides |
| description |
En Colombia se producen alrededor de 9 millones de toneladas de residuos de la cosecha de la caña de azúcar por hectárea cada año, que no han sido valorizados hacia ningún tipo de producto. Es necesario conocer su composición, en especial el contenido de hemicelulosas, para dilucidar el proceso para obtener los monosacáridos de éstas y aislarlos eficientemente. Para separar las hemicelulosas puras, se estudiaron cinco procesos de extracción a partir de las hojas y cogollos de las variedades de caña de azúcar CC 8592, CC 8475 y V 7151. Después de evaluar la eliminación de ceras con etanol y mezclas de cloroformo-metanol, los residuos se expusieron a diferentes concentraciones de peróxido de hidrógeno y clorito de sodio acidificado para lograr su deslignificación. Adicionalmente, se desarrollaron procedimientos de extracción con hidróxido de sodio e hidróxido de potasio, y de precipitación. El método que logró el mayor rendimiento fue el de desceración con metanol-cloroformo y deslignificación con clorito de sodio y borohidruro de sodio, con un rango de recuperación entre 0.20-0.34 g hemicelulosas /g residuo seco en las variedades estudiadas |
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2018 |
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2018 |
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2019-11-07T23:48:02Z |
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2019-11-07T23:48:02Z |
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Artículo de revista |
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27 |
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Flórez-Pardo, L. M., González-Córdoba, A., & López-Galán, J. E. (2018). Evaluation of different methods for efficient extraction of hemicelluloses leaves and tops of sugarcane. Dyna, 85(204), 18-27 |
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Dyna |
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[1] Farhat, W., Venditti, R., Quick, A., Taha, M., Mignard, N., Becquart, F., et al., Hemicellulose extraction and characterization for applications in paper coatings and adhesives. Ind Crops Prod [Online]. 107(January), 2017 [cited: July 24th of 2017]. Available at: DOI: 10.1016/j.indcrop.2017.05.055 [2] Sánchez, O.J. and Cardona, C., Trends in biotechnological production of fuel ethanol from different feedstocks. Bioresour Technol [Online]. 99(13), pp. 5270-5295, 2008 [cited: 2014 May 26]. Available at: https://www.ncbi.nlm.nih.gov/pubmed/18158236. DOI: 10.1016/j.biortech.2007.11.013 [3] FAO. Food and agriculture organization of the United Nations statistics division [Online]. Trade/Crops Livest. Prod. 2016 [cited: 2017 Jul 24]. Available at: http://faostat3.fao.org/browse/T/TP/E [4] Morais de Carvalho, D., Abad, A.M., Evtuguin, D.V., Colodette, J.L., Lindström, M.E., Vilaplana, F., et al., Isolation and characterization of acetylated glucuronoarabinoxylan from sugarcane bagasse and straw. Carbohydr Polym [Online]. 156, pp. 223-234, 2017 [cited 2017 Jul 24]. Available at: DOI: 10.1016/j.carbpol.2016.09.022 [5] Sun, R.C., Tomkinson J., Ma, P.L. and Liang, S.F., Comparative study of hemicelluloses from rice straw by alkali and hydrogen peroxide treatments. Carbohydr Polym [Online]. 42(2000), pp. 111-122, Jun, 2000. [cited: 2014 May 26]. Available at: http://linkinghub.elsevier.com/retrieve/pii/ S0144861799001368 [6] Pérez, S. and Samain, D., Structure and engineering of celluloses. Adv Carbohydr Chem Biochem [Online]. 64, pp. 25-116, 2010 [cited 2014 Jun 3]. DOI: 10.1016/S0065-2318(10)64003-6 [7] Geng, Z.C., Sun, R.C., Sun, X.F. and Lu, Q., Comparative study of hemicelluloses released during two-stage treatments with acidic organosolv and alkaline peroxide from Caligonum monogoliacum and Tamarix spp. Polym Degrad Stab. [Online]. 80(2), pp. 315-325, 2003. [cited 2014 May 28]. Available at: DOI: 10.1016/S0141-3910(03)00015-6 [8] Escalante, H., Orduz, J., Zapata, H., Cardona, M.C. y Duarte, M., Atlas del potencial energético de la biomasa residual en Colombia. Bucaramanga, Universidad Industrial de Santander, UPME, Ideam, Colciencias, 2010, 163 P. [9] Kang, L., Lee, Y.Y., Yoon, S.H., Smith, A.J. and Krishnagopalan, G.A., Ethanol production from the mixture of hemicellulose prehydrolysate and paper sludge. BioResources [Online]. 7(3), pp. 3607-3626, 2012. [cited 2014 May 28]. Available at: http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_07_3_3607_Kang_LYSK_Ethanol_Hemicellulose_Paper_Sludge [10] Sun, J.X., Sun, X.F., Sun, R.C. and Su, YQ., Fractional extraction and structural characterization of sugarcane bagasse hemicelluloses. Carbohydr Polym [Online]. 56(2), pp. 195-204, 2004. [cited 2014 May 23]. Available at: http://linkinghub.elsevier.com/retrieve/pii/ S0144861704000384 [11] Brienzo, M., Siqueira, A.F. and Milagres, A.M., Search for optimum conditions of sugarcane bagasse hemicellulose extraction. Biochem Eng. J. [Online]. 46(2), pp. 199-204, 2009. [cited 2016 Mar 21]. Available at: DOI: 10.1016/j.bej.2009.05.012 [12] Sluiter, A., Hames, B., Hyman, D., Payne, C., Ruiz, R., Scarlata, C., et al., Determination of total solids in biomass and total dissolved solids in liquid process samples. Laboratory Analytical Procedure (LAP). Golden, CO; 2008. [13] Nadia, H., Bchir, B., Blecker, C., Paquot, M. and Wathelet, B., Comparative study of alkaline extraction process of hemicelluloses from pear pomace. Biomass and Bioenergy [Online]. 61, 2014. [cited 2016 Mar 21]. Available at: DOI: 10.1016/j.biombioe.2013.12.022 [14] Xiao, B., Sun, X. and Sun, R., Chemical, structural, and thermal characterizations of alkali-soluble lignins and hemicelluloses, and cellulose from maize stems, rye straw, and rice straw. Polym Degrad Stab [Online]. 74(2), pp. 307-319, 2001. [cited 2014 May 23]. Available at: http://linkinghub.elsevier.com/retrieve/pii/ S014139100100163X [15] Doner, L.W. and Hicks, K.B., Isolation of hemicellulose from corn fiber by alkaline hydrogen peroxide extraction. Cereal Chem [Online]. 74(2), pp. 176-181, 1997. [cited 2014 Jun 3]. Available at: http://www.aaccnet.org/publications/cc/1997/March/Pages/74_2_176.aspx [16] Collings, G.F., Yokoyama, M.T. and Bergen, W.G., Lignin as determined by oxidation with sodium chlorite and a comparison with permanganate lignin. J Dairy Sci Elsevier. [Online]. 61(8), pp. 1156-1160, 1978. [cited 2014 Jun 3]. Available at: http://www.journalofdairyscience.org/article/S0022-0302(78)83700-X/abstract [17] Flórez L.M., Diseño de un complejo enzimático adaptado a la licuefacción de frutas tropicales. Aplicación a la pulpa de maracuyá. Tesis Dr., Universidad Politécnica de Valencia, España, 2002, pp. 113-114. [18] Höije, A., Gröndahl, M., Tømmeraas, K. and Gatenholm, P., Isolation and characterization of physicochemical and material properties of arabinoxylans from barley husks. Carbohydr. Polym. [Online]. 61(3), pp. 266-275, 2005 [cited 2014 May 28]. Available at: http://linkinghub.elsevier.com/retrieve/pii/S0144861705000706 [19] Casey, J.P., Ed.. Pulpa y papel. Química y tecnología química. México, D.F., Limusa, 1990. 32 P. [20] Sun, R.C., Fang, J.M., Tomkinson, J., Geng, Z.C. and Liu, J.C., Fractional isolation, physico-chemical characterization and homogeneous esterification of hemicelluloses from fast-growing poplar wood. Carbohydr Polym. [Online]. 44(1), pp. 29-39, 2001 [cited 2014 May 29]. Available at: DOI: 10.1016/S0144-8617(00)00196-X [21] Dumitriu, S., Polysaccharides. Structural diversity and functional versatility [Online]. 2nd ed. New York, Marcel Dekker, 2005 [cited 2014 Jun 3]. pp. 476, 484-485, 493, 1000-1001. Available at: http://books.google.com/books?id=kvUTPxPbkowC&pgis=1 [22] Sun, S.L., Wen, J.L., Ma, M.G., Sun, R.C., Successive alkali extraction and structural characterization of hemicelluloses from sweet sorghum stem. Carbohydr Polym [Online]. 92(2), pp. 2224-2231, 2013 [cited 2014 Jun 11]. Available at: http://www.ncbi.nlm.nih.gov/pubmed/23399281 [23] Methacanon, P., Chaikumpollert, O., Thavorniti, P. and Suchiva, K., Hemicellulosic polymer from Vetiver grass and its physicochemical properties. Carbohydr Polym [Online]. 54(3), pp. 335-342, 2003 [cited 2014 May 28]. Available at: DOI: 10.1016/S0144-8617(03)00182-6 [24] Attard, T.M., McElroy, C.R., Rezende, C., Polikarpov, I., Clark, J.H. and Hunt, A.J., Sugarcane waste as a valuable source of lipophilic molecules. Ind Crops Prod. [Online]. 76, pp. 95-103, 2015. Available at: http://linkinghub.elsevier.com/retrieve/pii/S0926669015301618 [25] Voragen, A.C., Rolin, C., Marr, B.U., Challen, I., Riad, A., Lebbar, R., et al., Polysaccharides. Ullmann’s Encyclopedia of Industrial Chemistry. 6th ed. Weinheim, Germany, Wiley-VCH Verlag GmbH & Co. KGaA, 2003, pp. 189-195. [26] Guillotin, S.E., Loey, A., Boulenguer, P., Schols, H.A. and Voragen, A.G., Rapid HPLC method to screen pectins for heterogeneity in methyl-esterification and amidation. Food Hydrocoll. [Online]. 21, 2007. [cited 2014 Jun 3]. Available at: http://www.wageningenur.nl/fr/Publications.htm?publicationId=publication-way-333631363032. DOI: 10.1016/j.foodhyd.2006.02.003 [27] Kurz, C., Carle, R. and Schieber, A., Characterisation of cell wall polysaccharide profiles of apricots (Prunus armeniaca L.), peaches (Prunus persica L.), and pumpkins (Cucurbita sp.) for the evaluation of fruit product authenticity. Food Chem. [Online]. 106(1), pp. 421-443, 2008. [cited 2014 May 28]. Available at: http://linkinghub.elsevier.com/retrieve/pii/S0308814607005407 [28] Sun, R.C., Tomkinson, J., Wang, Y.X. and Xiao, B., Physico-chemical and structural characterization of hemicelluloses from wheat straw by alkaline peroxide extraction. Polymer [Online]. 41(7), pp. 2647-2656, 2000. [cited 2014 May 28]. Available at: DOI: 10.1016/S0032-3861(99)00436-X [29] Gómez, C.H., Caracterización fisicoquímica de los polisacáridos presentes en los residuos de cosecha (hojas y cogollos) de tres variedades (CC 8475, CC 8592, V 7151) de caña de azúcar (Saccharum officinarum L.), Tesis MSc., Universidad del Valle, Cali, Colombia, 2010, pp. 32-52. [30] Kumar, R., Hu, F., Hubbell, C., Ragauskas, A.J. and Wyman, C.E., Comparison of laboratory delignification methods, their selectivity, and impacts on physiochemical characteristics of cellulosic biomass. Bioresour Technol. [Online]. 130, pp. 372-381, 2013. [cited 2014 Jun 3]. Available at: http://www.ncbi.nlm.nih.gov/pubmed/23313683 [31] Zhou, Y., Stuart-Williams, H., Farquhar, G.D. and Hocart, C.H., The use of natural abundance stable isotopic ratios to indicate the presence of oxygen-containing chemical linkages between cellulose and lignin in plant cell walls. Phytochemistry. [Online]. 71(8–9), pp. 982-993, 2010. [cited 2014 May 28]. Available at: http://www.ncbi.nlm.nih.gov/pubmed/2036 982-932306. DOI: 10.1016/j.phytochem.2010.03.001 [32] Hubbell, C.A. and Ragauskas, A.J., Effect of acid-chlorite delignification on cellulose degree of polymerization. Bioresour Technol. [Online]. 101(19), pp. 7410-7415, 2010. [cited 2014 May 28]. Available at: http://www.ncbi.nlm.nih.gov/pubmed/20471250. DOI: 10.1016/j.biortech.2010.04.029. [33] Xu, F., Sun, J., Geng, Z., Liu, C., Ren, J., Sun, R., et al., Comparative study of water-soluble and alkali-soluble hemicelluloses from perennial ryegrass leaves (Lolium peree). Carbohydr Polym. [Online]. 67(1), pp. 56-65, 2007. [cited 2014 May 28]. Available at: http://linkinghub.elsevier.com/retrieve/pii/S0144861706002141 [34] Carpita, N.C., Ralph, J. and McCann, M.C., Biochemistry and molecular biology of plants. [Online]. 2nd ed. Buchanan, B., Gruissem, W. and Jones, R., Eds. Rockville, John Wiley & Sons, 2015 [cited 2017 Jul 24]. pp. 51, 65, 73, 78. Available at: https://books.google.com.co/books?id=9YAZCgAAQBAJ&printsec=frontcover&hl=es#v=onepage&q&f=false [35] Gírio, F.M., Fonseca, C., Carvalheiro, F., Duarte, L.C., Marques, S. and Bogel-Łukasik, R., Hemicelluloses for fuel ethanol. A review. Bioresour Technol. [Online]. 101(13), pp. 4775-4800. 2010. [cited 2014 May 24]. Available at: https://www.ncbi.nlm.nih.gov/pubmed/20171088. DOI: 10.1016/j.biortech.2010.01. [36] Peng, Y. and Wu, S., The structural and thermal characteristics of wheat straw hemicellulose. J Anal Appl Pyrolysis, [Online]. 88(2), pp. 103-214, 2010. [cited 2014 May 28]. Available at: http://linkinghub.elsevier.com/retrieve/pii/S0165237010000392 [37] Bian, J., Peng, F., Peng, X.P., Xu, F., Sun, R.C. and Kennedy, J.F., Isolation of hemicelluloses from sugarcane bagasse at different temperatures: Structure and properties. Carbohydr Polym. [Online]. 88(2), pp. 638-645, 2012 [cited 2014 May 23]. Available at: http://linkinghub.elsevier.com/retrieve/pii/S0144861712000124. DOI: 10.1016/j.carbpol.2012.01.010 [38] Ebringerová, A. and Thomas, H., Hemicellulose. In: Heinze T, Ed.. Polysaccharides I Structure, Characterization and Use. Heidelberg, Springer Berlin Heidelberg, 2005, pp. 1–67. [39] Frei, M., Lignin: Characterization of a multifaceted crop component. The Scientific World Journal. [Online]. 2013, ID 436517, 25 P. [cited 2014 May 23]. Available at: DOI: 10.1155/2013/436517 [40] Pesquet, E., Zhang, B., Gorzsás, A., Puhakainen, T., Serk, H., Escamez, S., et al., Non-Cell-autonomous postmortem lignification of tracheary elements in Zinnia elegans. Plant Cell [Online]. 25(4), pp. 1314-1328, 2013. Available at: https://www.ncbi.nlm.nih.gov/pubmed/23572543. DOI: 10.1105/tpc.113.110593. [41] Brienzo, M., Abud, Y., Ferreira, S., Corrales, R.C., Ferreira-leitão, V.S., Souza, W., et al., Characterization of anatomy, lignin distribution, and response to pretreatments of sugarcane culm node and internode. Ind Crop Prod. [Online]. 84, pp. 305-313, 2016. Available at: DOI: 10.1016/j.indcrop.2016.01.039. |
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Flórez Pardo, Luz Marinavirtual::1696-1López Galán, Jorge Enrique430f1791219d95c12c9e39e5b11c76d8González-Córdoba, Andreaee4a4beaa8da5d74f8fe54b200558dd1Universidad Autónoma de Occidente. Calle 25 115-85. Km 2 vía Cali-Jamundí2019-11-07T23:48:02Z2019-11-07T23:48:02Z20182346-2183 (en línea)0012-7353 (impresa)http://hdl.handle.net/10614/11434En Colombia se producen alrededor de 9 millones de toneladas de residuos de la cosecha de la caña de azúcar por hectárea cada año, que no han sido valorizados hacia ningún tipo de producto. Es necesario conocer su composición, en especial el contenido de hemicelulosas, para dilucidar el proceso para obtener los monosacáridos de éstas y aislarlos eficientemente. Para separar las hemicelulosas puras, se estudiaron cinco procesos de extracción a partir de las hojas y cogollos de las variedades de caña de azúcar CC 8592, CC 8475 y V 7151. Después de evaluar la eliminación de ceras con etanol y mezclas de cloroformo-metanol, los residuos se expusieron a diferentes concentraciones de peróxido de hidrógeno y clorito de sodio acidificado para lograr su deslignificación. Adicionalmente, se desarrollaron procedimientos de extracción con hidróxido de sodio e hidróxido de potasio, y de precipitación. El método que logró el mayor rendimiento fue el de desceración con metanol-cloroformo y deslignificación con clorito de sodio y borohidruro de sodio, con un rango de recuperación entre 0.20-0.34 g hemicelulosas /g residuo seco en las variedades estudiadasThere are around 9 million tons of sugarcane crop residue per year in Colombia that are not recovered but they have potential to make value-added products. It is necessary to know the residue components, especially those from hemicellulose, to clarify the type of process to obtain the hemicellulose monosaccharides and isolate them efficiently. In order to separate pure hemicelluloses, five processes of extraction from tops and leaves of CC 8592, CC 8475, and V 7151 sugarcane varieties were studied. After evaluating exposure to dewaxing using ethanol and chloroform-methanol mixtures, residues were subjected to different concentrations of hydrogen peroxide and acidified sodium chlorite to achieve delignification. Additionally, procedures of extraction were developed with sodium hydroxide, potassium hydroxide, and precipitation. Chloroform-methanol dewaxing and sodium chlorite with sodium borohydride delignification allowed to achieve the highest extraction yield, with hemicellulose recovery ranging from 0.20-0.34 g hemicellulose/g dry residue in the varieties studied.application/pdf10 páginasengUniversidad Nacional de Colombia (Sede Medellín). Facultad de MinasDerechos Reservados - Universidad Autónoma de Occidentehttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)http://purl.org/coar/access_right/c_abf2Evaluation of different methods for efficient extraction of hemicelluloses leaves and tops of sugarcaneEvaluación de diferentes métodos para la extracción eficiente de hemicelulosas de las hojas y cogollos de la caña de azúcarArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTREFinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Cultivos energéticosEnergy cropsBioetanolResiduo lignocelulósicoPolisacáridosBioethanolLignocellulosic residuePolysaccharides27204204188585Flórez-Pardo, L. M., González-Córdoba, A., & López-Galán, J. E. (2018). 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Available at: DOI: 10.1016/j.indcrop.2016.01.039.Publicationcc4b057a-0ef8-456a-bec2-3d4e0f299a5cvirtual::1696-1cc4b057a-0ef8-456a-bec2-3d4e0f299a5cvirtual::1696-1https://scholar.google.com/citations?user=88OyeaAAAAAJ&hl=es&oi=aovirtual::1696-10000-0001-8779-8120virtual::1696-1https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000002410virtual::1696-1CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805https://red.uao.edu.co/bitstreams/c9b446a4-355a-4098-91ae-d6708300a6cf/download4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81665https://red.uao.edu.co/bitstreams/0a037342-7c25-4ccd-871b-297e8063430a/download20b5ba22b1117f71589c7318baa2c560MD53ORIGINALEvaluation of different methods for efficient extraction of hemicelluloses leaves and tops of sugarcane.pdfEvaluation of different methods for efficient extraction of hemicelluloses leaves and tops of sugarcane.pdfTexto archivo completo del artículo de revista, PDFapplication/pdf549122https://red.uao.edu.co/bitstreams/5e822a4c-c587-4a9e-964c-f279359bf555/download8b41ac40302b4fa12ef35505299f7068MD54TEXTEvaluation of different methods for efficient extraction of hemicelluloses leaves and tops of sugarcane.pdf.txtEvaluation of different methods for efficient extraction of hemicelluloses leaves and tops of sugarcane.pdf.txtExtracted texttext/plain54439https://red.uao.edu.co/bitstreams/232742e6-b8cc-40c4-ada2-e37b91e30871/downloadcaf2e690242021368bd1ef312981df8dMD55THUMBNAILEvaluation of different methods for efficient extraction of hemicelluloses leaves and tops of sugarcane.pdf.jpgEvaluation of different methods for efficient extraction of hemicelluloses leaves and tops of sugarcane.pdf.jpgGenerated Thumbnailimage/jpeg16000https://red.uao.edu.co/bitstreams/51b59b28-ad20-4dac-b26d-02e97a22308c/downloadd00fdb1d78a406e65c27dcb065c3c8fbMD5610614/11434oai:red.uao.edu.co:10614/114342024-03-05 09:51:11.654https://creativecommons.org/licenses/by-nc-nd/4.0/Derechos Reservados - Universidad Autónoma de Occidenteopen.accesshttps://red.uao.edu.coRepositorio Digital Universidad Autonoma de Occidenterepositorio@uao.edu.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 |