Significant enzymatic activities in the residues hydrolysis of the sugar cane harvest

In the production of ethanol from agroindustrial crop residues, one of the critical stages in the process is the conversion of lignocellulosic material to simple sugars, which can be done chemically or enzymatically. In this research, the enzymatic activities of commercial enzymes were evaluated for...

Full description

Autores:
Salcedo Mendoza, Jairo Guadalupe
López Galán, Jorge Enrique
Flórez Pardo, Luz Marina
Tipo de recurso:
Article of journal
Fecha de publicación:
2019
Institución:
Universidad Autónoma de Occidente
Repositorio:
RED: Repositorio Educativo Digital UAO
Idioma:
eng
OAI Identifier:
oai:red.uao.edu.co:10614/13402
Acceso en línea:
https://hdl.handle.net/10614/13402
Palabra clave:
Hidrolisis
Hydrolysis
Tallo
Hojas
Caña de azúcar
Hemicelulasas
Celulasas
Hidrólisis enzimática
top cane
leaves
sugar cane
hemicellulose
cellulases
enzymatic hydrolysis
Rights
openAccess
License
Derechos reservados - Universidad Nacional de Colombia, Sede Medellín, 2019
Description
Summary:In the production of ethanol from agroindustrial crop residues, one of the critical stages in the process is the conversion of lignocellulosic material to simple sugars, which can be done chemically or enzymatically. In this research, the enzymatic activities of commercial enzymes were evaluated for their influence on the degradation of lignocellulosic materials from sugar cane harvest residues (leaves and top cane). Eight substrates were pretreated with different delignification methods. Likewise, five enzymatic preparations were configured. An analysis of the enzyme-substrate interactions was conducted through fuzzy system analysis. The results showed regions of maximum enzymatic activity for residues of the sugarcane harvest, between 20-30 Filter Paper Units (FPU) /mL values lower than 500 pNPG (p-Nitrofenol-α-D-Glucopyranoside) U / mL of activity beta-glucosidase and hemicellulase activity between 50 and 70 IU / mL, confirming that the use of large amounts of cellulolytic enzymes is not necessary