Using a mediator system to increase the delignification of sugarcane residues with fungal enzymes

Los residuos industriales son recursos que generan una seguridad energética regional, pero ellos no han sido suficientemente valorizados. En el sur de Colombia, el sector de la caña de azúcar produce aproximadamente 9´000 000 T/año de residuos, principalmente representados por hojas y cogollos. De e...

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Autores:: Flórez Pardo, Luz Marina
Parra Paz, Angela Sofía
López Galán, Jorge Enrique
Figueroa Oviedo, Jersson Ivan

Tipo de recurso:: Article of journal

Fecha de publicación:: 2015

Institución:: Universidad Autónoma de Occidente

Repositorio:: RED: Repositorio Educativo Digital UAO

Idioma:: eng

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dc.title.eng.fl_str_mv	Using a mediator system to increase the delignification of sugarcane residues with fungal enzymes
dc.title.alternative.por.fl_str_mv	Uso de um sistema mediador para incrementar a deslignificação de resíduos de cana de açúcar com enzimas fúngicas
dc.title.alternative.spa.fl_str_mv	Uso de un sistema mediador para incrementar la deslignificación de residuos de caña de azúcar con enzimas fúngicas
title	Using a mediator system to increase the delignification of sugarcane residues with fungal enzymes
spellingShingle	Using a mediator system to increase the delignification of sugarcane residues with fungal enzymes Pleurotus ostreatus Lignoperoxidasa Enzymatic delignification Mediators Laccase Pleurotus ostreatus Lignoperoxidase Ultrasound pretreatment Tops and leaves Deslignificación enzimática Mediadores Lacasa Tratamiento con ultrasonido Hojas y cogollos Factory and trade waste - Recycling Sugar - Manufacture and refining Industria azucarera Conversión de residuos industriales Energy conservation Salvage (Waste, etc.) Aprovechamiento de residuos Conservación de la energía
title_short	Using a mediator system to increase the delignification of sugarcane residues with fungal enzymes
title_full	Using a mediator system to increase the delignification of sugarcane residues with fungal enzymes
title_fullStr	Using a mediator system to increase the delignification of sugarcane residues with fungal enzymes
title_full_unstemmed	Using a mediator system to increase the delignification of sugarcane residues with fungal enzymes
title_sort	Using a mediator system to increase the delignification of sugarcane residues with fungal enzymes
dc.creator.fl_str_mv	Flórez Pardo, Luz Marina Parra Paz, Angela Sofía López Galán, Jorge Enrique Figueroa Oviedo, Jersson Ivan
dc.contributor.author.none.fl_str_mv	Flórez Pardo, Luz Marina
dc.contributor.author.spa.fl_str_mv	Parra Paz, Angela Sofía López Galán, Jorge Enrique Figueroa Oviedo, Jersson Ivan
dc.subject.eng.fl_str_mv	Pleurotus ostreatus Lignoperoxidasa Enzymatic delignification Mediators Laccase Pleurotus ostreatus Lignoperoxidase Ultrasound pretreatment Tops and leaves
topic	Pleurotus ostreatus Lignoperoxidasa Enzymatic delignification Mediators Laccase Pleurotus ostreatus Lignoperoxidase Ultrasound pretreatment Tops and leaves Deslignificación enzimática Mediadores Lacasa Tratamiento con ultrasonido Hojas y cogollos Factory and trade waste - Recycling Sugar - Manufacture and refining Industria azucarera Conversión de residuos industriales Energy conservation Salvage (Waste, etc.) Aprovechamiento de residuos Conservación de la energía
dc.subject.spa.fl_str_mv	Deslignificación enzimática Mediadores Lacasa Tratamiento con ultrasonido Hojas y cogollos
dc.subject.lemb.eng.fl_str_mv	Factory and trade waste - Recycling Sugar - Manufacture and refining
dc.subject.lemb.spa.fl_str_mv	Industria azucarera Conversión de residuos industriales
dc.subject.armarc.eng.fl_str_mv	Energy conservation Salvage (Waste, etc.)
dc.subject.armarc.spa.fl_str_mv	Aprovechamiento de residuos Conservación de la energía
description	Los residuos industriales son recursos que generan una seguridad energética regional, pero ellos no han sido suficientemente valorizados. En el sur de Colombia, el sector de la caña de azúcar produce aproximadamente 9´000 000 T/año de residuos, principalmente representados por hojas y cogollos. De estos residuos es posible obtener etanol y una de las etapas más críticas es su deslignificación. En este proceso, la remoción de lignina con el uso de extractos de enzima extracelular y mediadores, todavía no ha sido ampliamente estudiada. Por consiguiente, se desarrolló una metodología para extraer del hongo Pleurotus ostreatus, el cual fue cultivado por fermentación en substrato sólido, un coctel de enzimas compuesto por lacasa, manganeso peroxidasa (MnP) y lignoperoxidasa (LiP). Estos extractos enzimáticos fueron probados con el uso de dos mediadores: 1) ABTS: 2,2´-azino-bis-3-etilbenzotiazolina-6-ácido sulfonico y 2) vainillina, sobre dos diferentes tipos de residuos: no tratados y pretratados con ultrasonido. Como resultado se encontró que el extracto crudo de enzima estuvo compuesto por actividad lacasa (0.432 U/mL), LiP (0.116 U/mL) y MnP (0.025 U/mL). Adicionalmente, los resultados confirmaron que este extracto crudo en asocio con ABTS como mediador de lacasa, fue capaz de remover el 52.7% de la lignina de los residuos de caña de azúcar pretratados con ultrasonido. También es importante resaltar que estos resultados fueron muy promisorios, sobre todo si se puede usar un extracto extracelular más concentrado.
publishDate	2015
dc.date.issued.spa.fl_str_mv	2015
dc.date.accessioned.spa.fl_str_mv	2019-11-26T18:48:16Z
dc.date.available.spa.fl_str_mv	2019-11-26T18:48:16Z
dc.type.spa.fl_str_mv	Artículo de revista
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dc.identifier.citation.spa.fl_str_mv	Flórez-Pardo, L. M., Parra-Paz, A. S., López-Galán, J. E., & Figueroa-Oviedo, J. I. (2015). Mediator system to increase the delignification of sugarcane residues with fungal enzymes. CT&F-Ciencia, Tecnología y Futuro, 6(2), 81-92
dc.identifier.issn.spa.fl_str_mv	0122-5383
dc.identifier.uri.spa.fl_str_mv	http://hdl.handle.net/10614/11577 http://www.scielo.org.co/scielo.php?script=sci_abstract&pid=S0122-53832015000200007 http://www.scielo.org.co/pdf/ctyf/v6n2/v6n2a07.pdf
identifier_str_mv	Flórez-Pardo, L. M., Parra-Paz, A. S., López-Galán, J. E., & Figueroa-Oviedo, J. I. (2015). Mediator system to increase the delignification of sugarcane residues with fungal enzymes. CT&F-Ciencia, Tecnología y Futuro, 6(2), 81-92 0122-5383
url	http://hdl.handle.net/10614/11577 http://www.scielo.org.co/scielo.php?script=sci_abstract&pid=S0122-53832015000200007 http://www.scielo.org.co/pdf/ctyf/v6n2/v6n2a07.pdf
dc.language.iso.eng.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	C.T.F. - Ciencia, Tecnología y Futuro, volumen 6, número 2, páginas 81-92, (diciembre, 2015)
dc.rights.spa.fl_str_mv	Derechos Reservados - Universidad Autónoma de Occidente
dc.rights.coar.fl_str_mv	http://purl.org/coar/access_right/c_abf2
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rights_invalid_str_mv	Derechos Reservados - Universidad Autónoma de Occidente https://creativecommons.org/licenses/by-nc-nd/4.0/ Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) http://purl.org/coar/access_right/c_abf2
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dc.format.extent.spa.fl_str_mv	páginas 81-92
dc.coverage.spatial.spa.fl_str_mv	Universidad Autónoma de Occidente. Calle 25 115-85. Km 2 vía Cali-Jamundí Colombia
dc.publisher.spa.fl_str_mv	Instituto Colombiano del Petróleo (ICP) - ECOPETROL S.A.
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dc.source.bibliographiccitation.spa.fl_str_mv	Bajpai, P. (2004). Biological bleaching of chemical pulps. Crit. Rev. Biotechnol., 24(1), 1-58 Benazzi, T., Calgaroto, S., Astolfi, V., Rosa, C. D., Oliveira, J. V. & Mazutti, M. A. (2013). Pretreatment of sugarcane bagasse using supercritical carbon dioxide combined with ultrasound to improve the enzymatic hydrolysis. Enzyme Microb. Technol., 52(4-5), 247-250 Bourbonnais, R. & Paice, M. (1992). Demethylation and delignification of kraft pulp by Trametes versicolor laccase in the presence of 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate). Appl. Microbiol. Biotechnol., 36(6), 823-827 Bourbonnais, R., Paice, M., Freiermuth, B., Bodie, E. & Borneman, S. (1997). Reactivities of various mediators and laccases with Kraft pulp and lignin model compounds. Appl. Environ. Microbiol., 63(12), 4627-4632 Camarero, S., Ibarra, D., Martínez, M. & Martínez, A. (2005). Lignin derived compounds as efficient laccase mediators for decolorization of different types of recalcitrant dyes. Appl. Environ. Microbiol., 71(4), 1775-1784 Cañas, A., Alcalde, M., Plou, F., Martínez, M., Martínez, A. & Camarero, S. (2007). Transformation of polycyclic aromatic hydrocarbons by lacasse is strongly enhanced by phenolic compounds present in soil. Environ. Sci. Technol., 41(8), 2964-2971 Cañas, A. & Camarero, S. (2010). Laccases and their natural mediators: Biotechnological tools for sustainable ecofriendly processes. Biotechnol. Adv., 28(6), 694-705 Cenicaña. (2010). Boletines diarios de la red meteorológica automatizada-RMA. [Online]. [Accessed: 03-Jun-2014]. Available from: http://www.cenicana.org/clima_/boletin_meteoro_diario.php Elisashvili, V. & Kachlishvili, E. (2009). Physiological regulation of laccase and manganese peroxidase production by white-rot Basidiomycetes. J. Biotechnol., 144(1), 37-42 Filson, P. B. & Dawson-Andoh, B. E. (2009). Sono-chemical preparation of cellulose nanocrrystals from lignocellulose derived materials. Bioresource Technol., 100(7), 2259-2264 Flickinger, M. & Drew, S. (1999). Encyclopedia of bioprocess technology: Fermentation, biocatalysis and bioseparation. New York: John Wiley and Sons Fujian, X., Hongzhang, C. & Zuohu, L. (2001). Solid state production of lignin peroxidase (LiP) and manganese peroxidase (MnP) by Phanerochaete chrysosporium using steam-exploded straw as substrate. Bioresour. Technol., 80(2), 149-155 Fujii, K., Uemura, M., Hayakawa, C., Funakawa, S. & Kosaki, T. (2013). Environmental control of lignin peroxidase, manganese peroxidase, and laccase activities in forest floor layers in humid Asia. Soil Biol. Biochem., 57: 109-115 Garcia-Ubasart, J., Esteban, A., Vila, C., Roncero, M. B., Colom, J. F. & Vidal, T. (2011). Enzymatic treatments of pulp using laccase and hydrophobic compounds. Bioresour. Technol., 102(3), 2799-2803 Hartree, E. F. (1972). Determination of protein: A modification of the Lowry method that gives a linear photometric response. Anal. Biochem., 48(2), 422-427 Jeon, J. R., Murugesan, K., Kim, Y., Kim, E. & Chang, Y. (2008). Synergistic effect of laccase mediators on pentachlorophenol removal by Ganoderma lucidum laccase. Appl. Microbiol. Biotechnol., 81(4), 783-790 Karp, S. G., Faraco, V., Amore, A., Birolo, L., Giangrande, C., Soccol, V. T., Pandey, A. & Soccol, C. R. (2012). Characterization of laccase isoforms produced by Pleurotus ostreatus in solid state fermentation of sugarcane bagasse. Bioresour. Technol., 114: 735-739 Kudanga, T. & Le Roes-Hill, M. (2014). Laccase applications in biofuels production: Current status and future prospects. Appl. Microbiol. Biotechnol., 98(15), 6525-6542 Liu, L., Lin, Z., Zheng, T., Lin, L., Zheng, C., Lin, Z., Wang, S. & Wang, Z. (2009). Fermentation optimization and characterization of the laccase from Pleurotus ostreatus strain 10969. Enzyme Microb. Technol., 44(6-7), 426-433 Lowry, O. H., Rosebrough, N., Farr, A. L. & Randall, R. (1951). Protein measurement with the folin phenol reagent. J. Biol. Chem., 193: 265-275 Maté, D., García-Burgos, C., García-Ruiz, E., Ballesteros, A.O., Camarero, S. & Alcalde, M. (2010). Laboratory evolution of high-redox potential laccases. Chem. Biol., 17(9), 1030-1041 Morozova, O. V., Shumakovich, G. P., Shleev, S. V. & Yaropolov, Y. I. (2007). Laccase-mediator systems and their applications: A review. Appl. Biochem. Microbiol., 43(5), 523-535 Oliveira, F. M. V., Pinheiro, I. O., Souto-Maior, A. M., Martin, C., Gonçalves, A. R. & Rocha, G. J. M. (2013). Industrialscale steam explosion pretreatment of sugarcane straw for enzymatic hydrolysis of cellulose for production of second generation ethanol and value-added products. Bioresource Technol., 130: 168-173 Park, N. & Park, S. S. (2014). Purification and characterization of a novel laccase from Fomitopsis pinicola mycelia. Int. J. Biol. Macromol., 70: 583-589 Peng, F., Peng, P., Xu, F. & Sun, R. C. (2012). Fractional purification and bioconversion of hemicelluloses. Biotechnol. Adv., 30(4), 879-903 Piscitelli, A., Giardina, P., Mazzoni, C. & Sannia, G. (2005). Recombinant expression of Pleurotus ostreatus laccases in Kluyveromyces lactis and Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol., 29(4), 428-439 Reddy, G. V., Ravindra-Babu, P., Komaraiah, P., Roy, K. R. R. M. & Kothari, L. (2003). Utilization of banana waste for the production of lignolytic and cellulolytic enzymes by solid substrate fermentation using two Pleurotus species (P. ostreatus and P. sajor-caju). Process Biochem., 38(10), 1457-1462 Rocha, G. J. M., Gonçalves, A. R., Oliveira, B. R., Olivares, E. G. & Rossell, C. E. V. (2012). Steam explosión pretreatment reproduction and alkaline delignification reactions performed on a pilot scale with sugarcane bagasse for bioethanol production. Ind. Crop. Prod., 35(1), 274-279 Rodríguez Couto, S. & Sanromán, M. (2005). Application of solid-state fermentation to lignolytic enzyme production. Biochem. Eng. J., 22(3), 211-219 Salcedo, J. G., López, J. E. & Flórez, L. M. (2011). Evaluación de enzimas para la hidrólisis de residuos (hojas y cogollos) de la cosecha caña de azúcar. DYNA, 78(169), 182-190 Sluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluiter, J., Templeton, D. & Crocker, D. (2011). Determination of structural carbohydrates and lignin in biomass. Technical Report NREL/TP-510-42618. Golden, CO, 18 pp. Sluiter, A., Ruiz, R., Scarlata, C., Sluiter, J. & Templeton, D. (2008). Determination of extractives in biomass. Technical Report NREL/TP-510-42619. Golden, CO, 12 pp. Sun, J. X., Sun, R. C., Sun, X. F. & Su, Y. Q. (2004). Fractional and physico-chemical characterization of hemicelluloses from ultrasonic irradiated sugarcane bagasse. Carbohydr. Res., 339(2), 291-300 Sun, R. & Tomkinson, J. (2002). Comparative study of lignins isolated by alkali and ultrasound-assisted alkali extractions from wheat straw. Ultrason. Sonochem., 9(2), 85-93 Szczerbowski, D., Pitarelo, A. P., Zandoná Filho, A. & Pereira Ramos, L. (2014). Sugarcane biomass for biorefineries: Comparative composition of carbohydrate and non-carbohydrate components of bagasse and straw. Carbohydr. Polym., 114: 95-101 TAPPI. (1999). Technical Association of the Pulp and Paper Industry. Kappa number of pulp. T 236 om-99. Canada, 4p. Van Soest, P. J., Robertson, J. B. & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci., 74(10), 3583-3597 Velmurugan, R. & Muthukumar, K. (2012). Ultrasoundassisted alkaline pretreatment of sugarcane bagasse for fermentable sugar production: Optimization through response surface methodology. Bioresource Technol., 112: 293-299 Yachmenev, V., Condon, B., Klasson, T. & Lambert, A. (2009). Acceleration of the enzymatic hydrolysis of corn stover and sugar cane bagasse celluloses by low intensity uniform ultrasound. J. Biobased Mater. Bio., 3(1-7), 25-31 Yaldagard, M., Mortazavi, S. A. & Tabatabaie, F. (2008). The effect of ultrasound in combination with thermal treatment on the germinated barley´s alpha-amylase activity. Korean J. Chem. Eng., 25(3), 517-523
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spelling	Flórez Pardo, Luz Marinavirtual::1705-1Parra Paz, Angela Sofía58beb630857d7c9b65c47a1e76a26bc2-1López Galán, Jorge Enriquec28f842fdc151536a90f2149d7bd6b1f-1Figueroa Oviedo, Jersson Ivan8191bb0561b62762ba82fc73830c427e-1Universidad Autónoma de Occidente. Calle 25 115-85. Km 2 vía Cali-JamundíColombia2019-11-26T18:48:16Z2019-11-26T18:48:16Z2015Flórez-Pardo, L. M., Parra-Paz, A. S., López-Galán, J. E., & Figueroa-Oviedo, J. I. (2015). Mediator system to increase the delignification of sugarcane residues with fungal enzymes. CT&F-Ciencia, Tecnología y Futuro, 6(2), 81-920122-5383http://hdl.handle.net/10614/11577http://www.scielo.org.co/scielo.php?script=sci_abstract&pid=S0122-53832015000200007http://www.scielo.org.co/pdf/ctyf/v6n2/v6n2a07.pdfLos residuos industriales son recursos que generan una seguridad energética regional, pero ellos no han sido suficientemente valorizados. En el sur de Colombia, el sector de la caña de azúcar produce aproximadamente 9´000 000 T/año de residuos, principalmente representados por hojas y cogollos. De estos residuos es posible obtener etanol y una de las etapas más críticas es su deslignificación. En este proceso, la remoción de lignina con el uso de extractos de enzima extracelular y mediadores, todavía no ha sido ampliamente estudiada. Por consiguiente, se desarrolló una metodología para extraer del hongo Pleurotus ostreatus, el cual fue cultivado por fermentación en substrato sólido, un coctel de enzimas compuesto por lacasa, manganeso peroxidasa (MnP) y lignoperoxidasa (LiP). Estos extractos enzimáticos fueron probados con el uso de dos mediadores: 1) ABTS: 2,2´-azino-bis-3-etilbenzotiazolina-6-ácido sulfonico y 2) vainillina, sobre dos diferentes tipos de residuos: no tratados y pretratados con ultrasonido. Como resultado se encontró que el extracto crudo de enzima estuvo compuesto por actividad lacasa (0.432 U/mL), LiP (0.116 U/mL) y MnP (0.025 U/mL). Adicionalmente, los resultados confirmaron que este extracto crudo en asocio con ABTS como mediador de lacasa, fue capaz de remover el 52.7% de la lignina de los residuos de caña de azúcar pretratados con ultrasonido. También es importante resaltar que estos resultados fueron muy promisorios, sobre todo si se puede usar un extracto extracelular más concentrado.Industrial residues are resources that generate regional energy security but they have not been sufficiently valued. In southern Colombia, the sugar cane sector produces approximately 9´000 000 T/year of residues, mostly represented by tops and leaves. Delignification is a critical step in the process of obtaining ethanol from these residues. The removal of lignin using extracellular enzyme extracts and mediators has not been widely studied. Therefore, a methodology was developed to extract, a cocktail of enzymes consisting of laccase, manganese peroxidase (MnP) and lignoperoxidase (LiP) from Pleurotus ostreatus, which was cultivated by solid substrate fermentation. The extracts with two mediators: 1) ABTS: 2,2´-azino-bis-3- ethylbenzthiazoline-6-sulfonic-acid and 2) vanillin, were tested on two different types of residues: untreated residues and pretreated residues with ultrasound. It was found that the crude enzyme extract contained laccase (0.432 U/mL), LiP (0.116 U/mL) and MnP (0.025 U/mL) activity. Additionally, the results confirmed that this extract was capable of removing 52.7% of lignin from ultrasound pretreated sugar cane residues and ABTS as the laccase mediator. It is important to highlight that the results obtained were very promising mainly if a more concentrated extracellular extract can be used.Os resíduos industriais são recursos que geram uma segurança energética regional, mas eles não são suficientemente valorizados. No sul da Colômbia, o setor da cana de açúcar produz aproximadamente 9´000 000 T/ano de resíduos, principalmente representados por folhas e miolos. Destes resíduos podemos obter etanol e uma das etapas mais críticas é a deslignificação. Neste processo, a remoção de lignina com o uso de extratos de enzima extracelular e mediadores, ainda não foi amplamente estudada. Consequentemente, foi desenvolvida uma metodologia para a extração do fungo Pleurotus ostreatus, o qual foi cultivado por fermentação em substrato sólido, um coquetel de enzimas composto por lacase, manganês peroxidase (MnP) e lignoperoxidase (LiP). Estes extratos enzimáticos foram testados com o uso dos mediadores: 1) ABTS: 2,2´-azino-bis-3-etilbenzotiazoline-6-ácido sulfônico e 2) vanilina, sobre dois diferentes tipos de resíduos: não tratados e pré-tratados com ultrassom. Como resultado verificou-se que o extrato cru de enzima esteve composto por atividade lacase (0.432 U/mL), LiP (0.116 U/mL) e MnP (0.025 U/mL). Adicionalmente, os resultados confirmaram que esse extrato cru junto com o ABTS como mediador de lacase foi capaz de remover 52.7% da lignina dos resíduos de cana de açúcar pré-tratados com ultrassom. Também é importante salientar que estes resultados foram muito promissórios, designadamente, se é possível utilizar um extrato extracelular mais concentradoapplication/pdfpáginas 81-92engInstituto Colombiano del Petróleo (ICP) - ECOPETROL S.A.C.T.F. - Ciencia, Tecnología y Futuro, volumen 6, número 2, páginas 81-92, (diciembre, 2015)Derechos Reservados - Universidad Autónoma de Occidentehttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)http://purl.org/coar/access_right/c_abf2instname:Universidad Autónoma de Occidentereponame:Repositorio Institucional UAOBajpai, P. (2004). Biological bleaching of chemical pulps. Crit. Rev. Biotechnol., 24(1), 1-58Benazzi, T., Calgaroto, S., Astolfi, V., Rosa, C. D., Oliveira, J. V. & Mazutti, M. A. (2013). Pretreatment of sugarcane bagasse using supercritical carbon dioxide combined with ultrasound to improve the enzymatic hydrolysis. Enzyme Microb. Technol., 52(4-5), 247-250Bourbonnais, R. & Paice, M. (1992). Demethylation and delignification of kraft pulp by Trametes versicolor laccase in the presence of 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate). Appl. Microbiol. Biotechnol., 36(6), 823-827Bourbonnais, R., Paice, M., Freiermuth, B., Bodie, E. & Borneman, S. (1997). Reactivities of various mediators and laccases with Kraft pulp and lignin model compounds. Appl. Environ. Microbiol., 63(12), 4627-4632Camarero, S., Ibarra, D., Martínez, M. & Martínez, A. (2005). Lignin derived compounds as efficient laccase mediators for decolorization of different types of recalcitrant dyes. Appl. Environ. Microbiol., 71(4), 1775-1784Cañas, A., Alcalde, M., Plou, F., Martínez, M., Martínez, A. & Camarero, S. (2007). Transformation of polycyclic aromatic hydrocarbons by lacasse is strongly enhanced by phenolic compounds present in soil. Environ. Sci. Technol., 41(8), 2964-2971Cañas, A. & Camarero, S. (2010). 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Carbohydr. Polym., 114: 95-101TAPPI. (1999). Technical Association of the Pulp and Paper Industry. Kappa number of pulp. T 236 om-99. Canada, 4p.Van Soest, P. J., Robertson, J. B. & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci., 74(10), 3583-3597Velmurugan, R. & Muthukumar, K. (2012). Ultrasoundassisted alkaline pretreatment of sugarcane bagasse for fermentable sugar production: Optimization through response surface methodology. Bioresource Technol., 112: 293-299Yachmenev, V., Condon, B., Klasson, T. & Lambert, A. (2009). Acceleration of the enzymatic hydrolysis of corn stover and sugar cane bagasse celluloses by low intensity uniform ultrasound. J. Biobased Mater. Bio., 3(1-7), 25-31Yaldagard, M., Mortazavi, S. A. & Tabatabaie, F. (2008). The effect of ultrasound in combination with thermal treatment on the germinated barley´s alpha-amylase activity. Korean J. Chem. Eng., 25(3), 517-523Pleurotus ostreatusLignoperoxidasaEnzymatic delignificationMediatorsLaccasePleurotus ostreatusLignoperoxidaseUltrasound pretreatmentTops and leavesDeslignificación enzimáticaMediadoresLacasaTratamiento con ultrasonidoHojas y cogollosFactory and trade waste - RecyclingSugar - Manufacture and refiningIndustria azucareraConversión de residuos industrialesEnergy conservationSalvage (Waste, etc.)Aprovechamiento de residuosConservación de la energíaUsing a mediator system to increase the delignification of sugarcane residues with fungal enzymesUso de um sistema mediador para incrementar a deslignificação de resíduos de cana de açúcar com enzimas fúngicasUso de un sistema mediador para incrementar la deslignificación de residuos de caña de azúcar con enzimas fúngicasArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTREFinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Publicationcc4b057a-0ef8-456a-bec2-3d4e0f299a5cvirtual::1705-1cc4b057a-0ef8-456a-bec2-3d4e0f299a5cvirtual::1705-1https://scholar.google.com/citations?user=88OyeaAAAAAJ&hl=es&oi=aovirtual::1705-10000-0001-8779-8120virtual::1705-1https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000002410virtual::1705-1CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805https://red.uao.edu.co/bitstreams/08c1e5cc-13f6-4fc3-9642-bd1a706ed7ad/download4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81665https://red.uao.edu.co/bitstreams/520e482d-ba4f-4283-be0f-74b04943b0ca/download20b5ba22b1117f71589c7318baa2c560MD5310614/11577oai:red.uao.edu.co:10614/115772024-03-05 10:00:49.466https://creativecommons.org/licenses/by-nc-nd/4.0/Derechos Reservados - Universidad Autónoma de Occidentemetadata.onlyhttps://red.uao.edu.coRepositorio Digital Universidad Autonoma de Occidenterepositorio@uao.edu.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

Using a mediator system to increase the delignification of sugarcane residues with fungal enzymes

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