Scale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulation

Azospirillum brasilense has industrial significance as a growth promoter in plants of commercial interest -- However, there is no report in the literature disclosing a liquid product produced in pilot-scale bioreactors and is able to be stored at room temperature for more than 2 years -- The aim of this work was to scale up a process from a shake flask to a 10- L lab-scale and 1,000-L pilot-scale bioreactor for the production of plant growth-promoting bacterium A -- brasilense for a liquid inoculant formulation -- Furthermore, this work aimed to determine the shelf life of the liquid formulation stored at room temperature and to increase maize crops yield in greenhouses -- Under a constant oxygen mass transfer coefficient (KLa), a fermentation process was successfully scaled up from shake flasks to 10- and 1,000-L bioreactors -- A concentration ranging from 3.5 to 7.5×108 CFU/mL was obtained in shake flasks and bioreactors, and after 2 years stored at room temperature, the liquid formulation showed one order of magnitude decrease -- Applications of the cultured bacteria in maize yields resulted in increases of up to 95 % in corncobs and 70 % in aboveground biomass

• Autores: Villegas, Jesús; Trujillo Roldán, Mauricio Alberto; Blancas Cabrera, Abel; Gamboa Suasnavart, Ramsés Adolfo; García Cabrera, Ramsés I.; Acevedo Sánchez, Eduardo V.; Valdez Cruz, Norma Adriana; González Monterrubio, César Fernando; Martínez Salinas, Carlos; Marín Palacio, Luz Deisy
• Fecha de publicación: 2013
• Institución: Universidad EAFIT
• Repositorio: Repositorio EAFIT
• Idioma: eng
• OAI Identifier: oai:repository.eafit.edu.co:10784/7256
• Acceso en línea: http://hdl.handle.net/10784/7256
• Palabra clave: AZOSPIRILLUM; INOCULACIÓN; FERMENTACIÓN; TÉCNICAS DE CULTIVO; FIJACIÓN DEL NITRÓGENO; BIOREACTORES; CRECIMIENTO (BACTERIAS); Azospirillum; Fermentation; Culture techniques(Biology); Nitrogen - fixation; Bioreactors; Bacterial growth; Biomasa aérea; Concentración bacteriana; Rizobacterias; Biofertilizante
• License: Springer-Verlag Berlin Heidelberg 2013