Fengycin C produced by Bacillus subtilis EA-CB0015

Bacillus subtilis EA-CB0015 was isolated from the phyllosphere of a banana plant and tested for its potential to produce bioactive compounds against Mycosphaerella fijiensis -- Using a dual plate culture technique the cell-free supernatant of B. subtilis EA-CB0015 produced inhibition values of 89 ± 1% -- The active compounds were purified by solid-phase extraction and HPLC, and their primary structures determined using mass spectrometry and amino acid analysis -- A new fengycin isoform, fengycin C, with the amino acid sequence Glu-Orn-Tyr-Thr-Glu-Val-Pro-Gln-Thr-Ile was isolated -- The peptidic moiety differs from fengycin B at position 9 and from fengycin A at positions 6 and 9 -- The β-hydroxy fatty acyl chain is connected to the N-terminal of the decapeptide and can be saturated or unsaturated, ranging from 14 to 18 carbons -- The C-terminal residue of the peptidic moiety is linked to the tyrosine residue at position 3, forming the branching point of the acyl peptide and the eight-membered cyclic lactone

• Autores: Ceballos, Isabel; J. Mira, John; Argel, Luz Edith; Orduz Peralta, Sergio; Romero Tabarez, Magally; Villegas Escobar, Valeska
• Fecha de publicación: 2014
• Institución: Universidad EAFIT
• Repositorio: Repositorio EAFIT
• Idioma: eng
• OAI Identifier: oai:repository.eafit.edu.co:10784/7258
• Acceso en línea: http://hdl.handle.net/10784/7258
• Palabra clave: BACILLUS SUBTILIS; COMPUESTOS BIOACTIVOS; ESPECTROMETRÍA DE MASAS; AMINOÁCIDOS; HONGOS FITOPATÓGENOS; COMPUESTOS ORGANOCÍCLICOS; LACTONAS; Bacillus subtilis; Bioactive compounds; Mass spectrometry; Amino acids; Phytopathogenic fungi; Organic cyclic compounds; Lactones; Tirosina; Fengycin
• License: Copyright © 2013 The American Chemical Society and American Society of Pharmacognosy