Bacillus sp. strains and their inducible in vitro antagonism : a biochemical and molecular study

Discovering novel antibiotic substances from natural sources and revitalizing the pipeline for screenings of naturally sourced substances that could render new bioactive compounds, is a priority nowadays in the face of a world crisis of antimicrobial resistance. This research was focused on disclosi...

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Autores:
Sierra Zapata, Laura
Tipo de recurso:
Fecha de publicación:
2018
Institución:
Universidad EAFIT
Repositorio:
Repositorio EAFIT
Idioma:
spa
OAI Identifier:
oai:repository.eafit.edu.co:10784/13835
Acceso en línea:
http://hdl.handle.net/10784/13835
Palabra clave:
Antibióticos
Metabolitos antimicrobianos
ANTIBIÓTICOS
Antibiotics
Antimicrobial metabolites
Rights
License
Acceso abierto
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network_name_str Repositorio EAFIT
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dc.title.spa.fl_str_mv Bacillus sp. strains and their inducible in vitro antagonism : a biochemical and molecular study
title Bacillus sp. strains and their inducible in vitro antagonism : a biochemical and molecular study
spellingShingle Bacillus sp. strains and their inducible in vitro antagonism : a biochemical and molecular study
Antibióticos
Metabolitos antimicrobianos
ANTIBIÓTICOS
Antibiotics
Antimicrobial metabolites
title_short Bacillus sp. strains and their inducible in vitro antagonism : a biochemical and molecular study
title_full Bacillus sp. strains and their inducible in vitro antagonism : a biochemical and molecular study
title_fullStr Bacillus sp. strains and their inducible in vitro antagonism : a biochemical and molecular study
title_full_unstemmed Bacillus sp. strains and their inducible in vitro antagonism : a biochemical and molecular study
title_sort Bacillus sp. strains and their inducible in vitro antagonism : a biochemical and molecular study
dc.creator.fl_str_mv Sierra Zapata, Laura
dc.contributor.advisor.spa.fl_str_mv Villegas Escobar, Valeska
Romero Tabarez, Magally
dc.contributor.author.none.fl_str_mv Sierra Zapata, Laura
dc.subject.spa.fl_str_mv Antibióticos
Metabolitos antimicrobianos
topic Antibióticos
Metabolitos antimicrobianos
ANTIBIÓTICOS
Antibiotics
Antimicrobial metabolites
dc.subject.lemb.spa.fl_str_mv ANTIBIÓTICOS
dc.subject.keyword.spa.fl_str_mv Antibiotics
Antimicrobial metabolites
description Discovering novel antibiotic substances from natural sources and revitalizing the pipeline for screenings of naturally sourced substances that could render new bioactive compounds, is a priority nowadays in the face of a world crisis of antimicrobial resistance. This research was focused on disclosing an observed antagonism system composed of Bacillus sp. strains producing inducible antimicrobial activity against the plant pathogen Ralstonia solanacearum, a widespread bacterium that causes bacterial wilt disease to a great variety of plant species, including many agriculturally important ones as are bananas. The inducible phenomenon was discovered during the screening of 1493 aerobic endospore forming bacteria against plant pathogens. It was observed that in the presence of the chemical compound Triphenyl Tetrazolium Chloride (TTC), which belongs to the group of synthetic compounds known as tetrazolium salts used to monitor cell respiration, Bacillus sp. strains produced inhibition zones against the bacterial plant pathogen and other pathogenic bacterial species, while in the absence of the compound they did not have any bioactivity. During biochemical characterization, it was evidenced that although the phenomenon was observable across several species of the order Bacillales, strains belonging to B. cereus, B. pumilus and B. subtilis were outstanding in their inducible antagonism potential, among other species tested. Besides, relevant traits revealed that other tetrazolium salts did not induce antagonistic activity and that the addition of antioxidant compounds did not reduce the inducible antagonistic activity. Also, R. solanacearum sensitivity to antibiotics was not increased by the addition of TTC and the inducible activity was independent of the presence of the pathogenic strain. In order to determine genes and pathways that were activated under TTC conditions, transcriptomic and metabolomics analysis were performed. Transcriptomic results revealed that specific pathways of the nitrogen metabolism, such as pyrimidines, purines and histidine biosynthetic routes, were 2 to 5 fold up-regulated in B. subtilis NCIB-3610 cells growing under TTC presence. On the other hand, metabolomic analysis showed that 28 specific compounds were either unique or 3 to 5 fold more abundant in active extracts obtained from inducible conditions, compared to non-induced controls. Data mining on public chemical databases, using intrinsic properties of the selected compounds, suggests that they mostly belong to chemical families of carbamates, imidazoles, pyrrolidines, pyrimidines, dipeptides and oligopeptides, all of which are part of the nitrogen metabolism. Results suggest that Bacillus cells reduction of TTC into triphenyl formazan (TPF) and its further accumulation inside the cells, induces the production of nitrogen-derived compounds, either by activation of nitrogen metabolism biosynthetic pathways or by a biotransformation of TPF into derivatives. Once produced, the compounds are secreted into the medium and act as antimicrobials against other bacteria.
publishDate 2018
dc.date.issued.none.fl_str_mv 2018
dc.date.available.none.fl_str_mv 2019-09-06T14:21:31Z
dc.date.accessioned.none.fl_str_mv 2019-09-06T14:21:31Z
dc.type.eng.fl_str_mv doctoralThesis
info:eu-repo/semantics/doctoralThesis
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_db06
dc.type.local.spa.fl_str_mv Tesis Doctoral
dc.type.hasVersion.eng.fl_str_mv acceptedVersion
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10784/13835
url http://hdl.handle.net/10784/13835
dc.language.iso.spa.fl_str_mv spa
language spa
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dc.rights.local.spa.fl_str_mv Acceso abierto
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dc.coverage.spatial.eng.fl_str_mv Medellín de: Lat: 06 15 00 N degrees minutes Lat: 6.2500 decimal degrees Long: 075 36 00 W degrees minutes Long: -75.6000 decimal degrees
dc.publisher.spa.fl_str_mv Universidad EAFIT
dc.publisher.program.spa.fl_str_mv Doctorado en Ingeniería
dc.publisher.department.spa.fl_str_mv Escuela de Ingeniería
dc.publisher.place.spa.fl_str_mv Medellín
institution Universidad EAFIT
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spelling Villegas Escobar, ValeskaRomero Tabarez, MagallySierra Zapata, LauraDoctor in Engineeringlsierra3@gmail.comMedellín de: Lat: 06 15 00 N degrees minutes Lat: 6.2500 decimal degrees Long: 075 36 00 W degrees minutes Long: -75.6000 decimal degrees2019-09-06T14:21:31Z20182019-09-06T14:21:31Zhttp://hdl.handle.net/10784/13835Discovering novel antibiotic substances from natural sources and revitalizing the pipeline for screenings of naturally sourced substances that could render new bioactive compounds, is a priority nowadays in the face of a world crisis of antimicrobial resistance. This research was focused on disclosing an observed antagonism system composed of Bacillus sp. strains producing inducible antimicrobial activity against the plant pathogen Ralstonia solanacearum, a widespread bacterium that causes bacterial wilt disease to a great variety of plant species, including many agriculturally important ones as are bananas. The inducible phenomenon was discovered during the screening of 1493 aerobic endospore forming bacteria against plant pathogens. It was observed that in the presence of the chemical compound Triphenyl Tetrazolium Chloride (TTC), which belongs to the group of synthetic compounds known as tetrazolium salts used to monitor cell respiration, Bacillus sp. strains produced inhibition zones against the bacterial plant pathogen and other pathogenic bacterial species, while in the absence of the compound they did not have any bioactivity. During biochemical characterization, it was evidenced that although the phenomenon was observable across several species of the order Bacillales, strains belonging to B. cereus, B. pumilus and B. subtilis were outstanding in their inducible antagonism potential, among other species tested. Besides, relevant traits revealed that other tetrazolium salts did not induce antagonistic activity and that the addition of antioxidant compounds did not reduce the inducible antagonistic activity. Also, R. solanacearum sensitivity to antibiotics was not increased by the addition of TTC and the inducible activity was independent of the presence of the pathogenic strain. In order to determine genes and pathways that were activated under TTC conditions, transcriptomic and metabolomics analysis were performed. Transcriptomic results revealed that specific pathways of the nitrogen metabolism, such as pyrimidines, purines and histidine biosynthetic routes, were 2 to 5 fold up-regulated in B. subtilis NCIB-3610 cells growing under TTC presence. On the other hand, metabolomic analysis showed that 28 specific compounds were either unique or 3 to 5 fold more abundant in active extracts obtained from inducible conditions, compared to non-induced controls. Data mining on public chemical databases, using intrinsic properties of the selected compounds, suggests that they mostly belong to chemical families of carbamates, imidazoles, pyrrolidines, pyrimidines, dipeptides and oligopeptides, all of which are part of the nitrogen metabolism. Results suggest that Bacillus cells reduction of TTC into triphenyl formazan (TPF) and its further accumulation inside the cells, induces the production of nitrogen-derived compounds, either by activation of nitrogen metabolism biosynthetic pathways or by a biotransformation of TPF into derivatives. Once produced, the compounds are secreted into the medium and act as antimicrobials against other bacteria.application/pdfspaUniversidad EAFITDoctorado en IngenieríaEscuela de IngenieríaMedellínAntibióticosMetabolitos antimicrobianosANTIBIÓTICOSAntibioticsAntimicrobial metabolitesBacillus sp. strains and their inducible in vitro antagonism : a biochemical and molecular studydoctoralThesisinfo:eu-repo/semantics/doctoralThesisTesis DoctoralacceptedVersionhttp://purl.org/coar/resource_type/c_db06Acceso abiertohttp://purl.org/coar/access_right/c_abf2LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repository.eafit.edu.co/bitstreams/81600c5a-cec8-433e-bf79-2b724b2ce3b0/download8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALLaura_SierraZapata_2018.pdfLaura_SierraZapata_2018.pdfTrabajo de gradoapplication/pdf40282069https://repository.eafit.edu.co/bitstreams/a9f6e9e9-e2f0-495f-93d0-38d179ab50d4/download12c552f6a64c410342281c592464382eMD5210784/13835oai:repository.eafit.edu.co:10784/138352022-12-13 08:56:02.398open.accesshttps://repository.eafit.edu.coRepositorio Institucional Universidad EAFITrepositorio@eafit.edu.co