Inducible Antibacterial Activity in the Bacillales by Triphenyl Tetrazolium Chloride

The world is in the midst of an antimicrobial resistance crisis, driving a need to discover novel antibiotic substances. Using chemical cues as inducers to unveil a microorganism’s full metabolic potential is considered a successful strategy. To this end, we investigated an inducible antagonistic be...

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Autores:
Sierra-Zapata L.
Álvarez J.C.
Romero-Tabarez M.
Silby M.W.
Traxler M.F.
Behie S.W.
Pessotti R.C.
Villegas-Escobar V.
Tipo de recurso:
Fecha de publicación:
2020
Institución:
Universidad EAFIT
Repositorio:
Repositorio EAFIT
Idioma:
eng
OAI Identifier:
oai:repository.eafit.edu.co:10784/26752
Acceso en línea:
https://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=10322
http://hdl.handle.net/10784/26752
Palabra clave:
Microbial
Natural-Products
Pseudomonas-Solanacearum
Mass-Spectrometry
Drug
Discovery
Gene
Clusters
In-Vitro
Bacterial
Lipopeptides
Metabolites
Resistance
Rights
License
https://v2.sherpa.ac.uk/id/publication/issn/2045-2322
id REPOEAFIT2_8ab2e34b41589ac6e4d8c3006c087f31
oai_identifier_str oai:repository.eafit.edu.co:10784/26752
network_acronym_str REPOEAFIT2
network_name_str Repositorio EAFIT
repository_id_str
spelling 2021-03-23T19:52:10Z2020-01-012021-03-23T19:52:10Zhttps://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=1032220452322WOS;000560406800011PUBMED;32221330SCOPUS;2-s2.0-85082544169http://hdl.handle.net/10784/2675210.1038/s41598-020-62236-zThe world is in the midst of an antimicrobial resistance crisis, driving a need to discover novel antibiotic substances. Using chemical cues as inducers to unveil a microorganism’s full metabolic potential is considered a successful strategy. To this end, we investigated an inducible antagonistic behavior in multiple isolates of the order Bacillales, where large inhibition zones were produced against Ralstonia solanacearum only when grown in the presence of the indicator triphenyl tetrazolium chloride (TTC). This bioactivity was produced in a TTC-dose dependent manner. Escherichia coli and Staphylococcus sp. isolates were also inhibited by Bacillus sp. strains in TTC presence, to a lesser extent. Knockout mutants and transcriptomic analysis of B. subtilis NCIB 3610 cells revealed that genes from the L-histidine biosynthetic pathway, the purine, pyrimidine de novo synthesis and salvage and interconversion routes, were significantly upregulated. Chemical space studied through metabolomic analysis, showed increased presence of nitrogenous compounds in extracts from induced bacteria. The metabolites orotic acid and L-phenylalaninamide were tested against R. solanacearum, E. coli, Staphylococcus sp. and B. subtilis, and exhibited activity against pathogens only in the presence of TTC, suggesting a biotransformation of nitrogenous compounds in Bacillus sp. cells as the plausible cause of the inducible antagonistic behavior. © 2020, The Author(s).engNature Publishing Grouphttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85082544169&doi=10.1038%2fs41598-020-62236-z&partnerID=40&md5=7f043140166225b91db2685f8989fbfehttps://v2.sherpa.ac.uk/id/publication/issn/2045-2322Acceso abiertohttp://purl.org/coar/access_right/c_abf2SCIENTIFIC REPORTSMicrobialNatural-ProductsPseudomonas-SolanacearumMass-SpectrometryDrugDiscoveryGeneClustersIn-VitroBacterialLipopeptidesMetabolitesResistanceInducible Antibacterial Activity in the Bacillales by Triphenyl Tetrazolium ChloridesubmittedVersioninfo:eu-repo/semantics/submittedVersionarticleinfo:eu-repo/semantics/articleArtículohttp://purl.org/coar/version/c_71e4c1898caa6e32http://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Universidad EAFIT. Departamento de CienciasSierra-Zapata L.Álvarez J.C.Romero-Tabarez M.Silby M.W.Traxler M.F.Behie S.W.Pessotti R.C.Villegas-Escobar V.Biodiversidad, Evolución y ConservaciónSCIENTIFIC REPORTS10784/26752oai:repository.eafit.edu.co:10784/267522022-04-27 15:49:59.753metadata.onlyhttps://repository.eafit.edu.coRepositorio Institucional Universidad EAFITrepositorio@eafit.edu.co
dc.title.eng.fl_str_mv Inducible Antibacterial Activity in the Bacillales by Triphenyl Tetrazolium Chloride
title Inducible Antibacterial Activity in the Bacillales by Triphenyl Tetrazolium Chloride
spellingShingle Inducible Antibacterial Activity in the Bacillales by Triphenyl Tetrazolium Chloride
Microbial
Natural-Products
Pseudomonas-Solanacearum
Mass-Spectrometry
Drug
Discovery
Gene
Clusters
In-Vitro
Bacterial
Lipopeptides
Metabolites
Resistance
title_short Inducible Antibacterial Activity in the Bacillales by Triphenyl Tetrazolium Chloride
title_full Inducible Antibacterial Activity in the Bacillales by Triphenyl Tetrazolium Chloride
title_fullStr Inducible Antibacterial Activity in the Bacillales by Triphenyl Tetrazolium Chloride
title_full_unstemmed Inducible Antibacterial Activity in the Bacillales by Triphenyl Tetrazolium Chloride
title_sort Inducible Antibacterial Activity in the Bacillales by Triphenyl Tetrazolium Chloride
dc.creator.fl_str_mv Sierra-Zapata L.
Álvarez J.C.
Romero-Tabarez M.
Silby M.W.
Traxler M.F.
Behie S.W.
Pessotti R.C.
Villegas-Escobar V.
dc.contributor.department.spa.fl_str_mv Universidad EAFIT. Departamento de Ciencias
dc.contributor.author.none.fl_str_mv Sierra-Zapata L.
Álvarez J.C.
Romero-Tabarez M.
Silby M.W.
Traxler M.F.
Behie S.W.
Pessotti R.C.
Villegas-Escobar V.
dc.contributor.researchgroup.spa.fl_str_mv Biodiversidad, Evolución y Conservación
dc.subject.eng.fl_str_mv Microbial
Natural-Products
Pseudomonas-Solanacearum
Mass-Spectrometry
Drug
Discovery
Gene
Clusters
In-Vitro
Bacterial
Lipopeptides
Metabolites
Resistance
topic Microbial
Natural-Products
Pseudomonas-Solanacearum
Mass-Spectrometry
Drug
Discovery
Gene
Clusters
In-Vitro
Bacterial
Lipopeptides
Metabolites
Resistance
description The world is in the midst of an antimicrobial resistance crisis, driving a need to discover novel antibiotic substances. Using chemical cues as inducers to unveil a microorganism’s full metabolic potential is considered a successful strategy. To this end, we investigated an inducible antagonistic behavior in multiple isolates of the order Bacillales, where large inhibition zones were produced against Ralstonia solanacearum only when grown in the presence of the indicator triphenyl tetrazolium chloride (TTC). This bioactivity was produced in a TTC-dose dependent manner. Escherichia coli and Staphylococcus sp. isolates were also inhibited by Bacillus sp. strains in TTC presence, to a lesser extent. Knockout mutants and transcriptomic analysis of B. subtilis NCIB 3610 cells revealed that genes from the L-histidine biosynthetic pathway, the purine, pyrimidine de novo synthesis and salvage and interconversion routes, were significantly upregulated. Chemical space studied through metabolomic analysis, showed increased presence of nitrogenous compounds in extracts from induced bacteria. The metabolites orotic acid and L-phenylalaninamide were tested against R. solanacearum, E. coli, Staphylococcus sp. and B. subtilis, and exhibited activity against pathogens only in the presence of TTC, suggesting a biotransformation of nitrogenous compounds in Bacillus sp. cells as the plausible cause of the inducible antagonistic behavior. © 2020, The Author(s).
publishDate 2020
dc.date.issued.none.fl_str_mv 2020-01-01
dc.date.available.none.fl_str_mv 2021-03-23T19:52:10Z
dc.date.accessioned.none.fl_str_mv 2021-03-23T19:52:10Z
dc.type.eng.fl_str_mv submittedVersion
info:eu-repo/semantics/submittedVersion
article
info:eu-repo/semantics/article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_71e4c1898caa6e32
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.local.spa.fl_str_mv Artículo
status_str submittedVersion
dc.identifier.none.fl_str_mv https://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=10322
dc.identifier.issn.none.fl_str_mv 20452322
dc.identifier.other.none.fl_str_mv WOS;000560406800011
PUBMED;32221330
SCOPUS;2-s2.0-85082544169
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10784/26752
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-020-62236-z
url https://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=10322
http://hdl.handle.net/10784/26752
identifier_str_mv 20452322
WOS;000560406800011
PUBMED;32221330
SCOPUS;2-s2.0-85082544169
10.1038/s41598-020-62236-z
dc.language.iso.eng.fl_str_mv eng
language eng
dc.relation.uri.none.fl_str_mv https://www.scopus.com/inward/record.uri?eid=2-s2.0-85082544169&doi=10.1038%2fs41598-020-62236-z&partnerID=40&md5=7f043140166225b91db2685f8989fbfe
dc.rights.none.fl_str_mv https://v2.sherpa.ac.uk/id/publication/issn/2045-2322
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.local.spa.fl_str_mv Acceso abierto
rights_invalid_str_mv https://v2.sherpa.ac.uk/id/publication/issn/2045-2322
Acceso abierto
http://purl.org/coar/access_right/c_abf2
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv SCIENTIFIC REPORTS
institution Universidad EAFIT
repository.name.fl_str_mv Repositorio Institucional Universidad EAFIT
repository.mail.fl_str_mv repositorio@eafit.edu.co
_version_ 1808498927183331328

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