Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.)

Objective: It is necessary to improve biotech platforms based on in vitro cell tissue culture to support sacha inchi (Plukenetia volubilis L.) research programs and draw on the nutritional value of the high polyunsaturated fatty acid content of its oilseed. Here, we developed a rapid and efficient m...

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Autores:
Restrepo-Osorio C.
Gil-Correal A.
Chamorro-Gutiérrez L.
Ramírez-Ríos V.
Álvarez J.C.
Villanueva-Mejía D.
Tipo de recurso:
Fecha de publicación:
2020
Institución:
Universidad EAFIT
Repositorio:
Repositorio EAFIT
Idioma:
eng
OAI Identifier:
oai:repository.eafit.edu.co:10784/26758
Acceso en línea:
https://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=12169
http://hdl.handle.net/10784/26758
Palabra clave:
Acclimatization
Adventitious
shoots
Oilseed
Rooting
Tissue
culture
Rights
License
https://v2.sherpa.ac.uk/id/publication/issn/1756-0500
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repository_id_str
spelling 2021-03-23T19:52:10Z2020-09-032021-03-23T19:52:10Zhttps://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=1216917560500PUBMED;32883361SCOPUS;2-s2.0-85090319012http://hdl.handle.net/10784/2675810.1186/s13104-020-05257-1Objective: It is necessary to improve biotech platforms based on in vitro cell tissue culture to support sacha inchi (Plukenetia volubilis L.) research programs and draw on the nutritional value of the high polyunsaturated fatty acid content of its oilseed. Here, we developed a rapid and efficient method for induction and direct in vitro shoot development for this species. Results: Shoots were generated from hypocotyl explants. The highest organogenic response was obtained in woody plant medium supplemented with 1 mg/L thidiazuron and 0.5 mg/L zeatin supplemented with L-glutamine, adenine hemisulfate, and L-arginine. Shoots obtained using this medium were transferred and subcultivated with different concentrations of indole-3-butyric acid and 1-naphthylacetic acid for rooting. For the first time, a histological analysis was performed supporting direct organogenic development in this species. The plantlets obtained were transferred ex vitro with a survival percentage of 80%. The genetic stability of the plants recovered was confirmed by randomly amplified polymorphic DNA analysis. All results indicate that it would be possible to stimulate direct shoot formation from hypocotyls to support the sustainable use of this species. © 2020 The Author(s).application/pdfengBioMed Central Ltd.https://www.scopus.com/inward/record.uri?eid=2-s2.0-85090319012&doi=10.1186%2fs13104-020-05257-1&partnerID=40&md5=17338532a6b5413b5901617efe0d4479https://v2.sherpa.ac.uk/id/publication/issn/1756-0500Acceso abiertohttp://purl.org/coar/access_right/c_abf2BMC Research NotesAcclimatizationAdventitiousshootsOilseedRootingTissuecultureEfficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.)publishedVersioninfo:eu-repo/semantics/publishedVersionarticleinfo:eu-repo/semantics/articleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Universidad EAFIT. Departamento de CienciasRestrepo-Osorio C.Gil-Correal A.Chamorro-Gutiérrez L.Ramírez-Ríos V.Álvarez J.C.Villanueva-Mejía D.Biodiversidad, Evolución y ConservaciónBMC Research NotesORIGINALs13104-020-05257-1.pdfs13104-020-05257-1.pdfTexto completoapplication/pdf1424591https://repository.eafit.edu.co/bitstreams/06ee00ba-4545-4f4a-85b8-a1efa0d7fee5/download7240776e68768a9b2e6e04cd2a611551MD5110784/26758oai:repository.eafit.edu.co:10784/267582022-04-27 15:50:00.343open.accesshttps://repository.eafit.edu.coRepositorio Institucional Universidad EAFITrepositorio@eafit.edu.co
dc.title.eng.fl_str_mv Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.)
title Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.)
spellingShingle Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.)
Acclimatization
Adventitious
shoots
Oilseed
Rooting
Tissue
culture
title_short Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.)
title_full Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.)
title_fullStr Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.)
title_full_unstemmed Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.)
title_sort Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.)
dc.creator.fl_str_mv Restrepo-Osorio C.
Gil-Correal A.
Chamorro-Gutiérrez L.
Ramírez-Ríos V.
Álvarez J.C.
Villanueva-Mejía D.
dc.contributor.department.spa.fl_str_mv Universidad EAFIT. Departamento de Ciencias
dc.contributor.author.none.fl_str_mv Restrepo-Osorio C.
Gil-Correal A.
Chamorro-Gutiérrez L.
Ramírez-Ríos V.
Álvarez J.C.
Villanueva-Mejía D.
dc.contributor.researchgroup.spa.fl_str_mv Biodiversidad, Evolución y Conservación
dc.subject.eng.fl_str_mv Acclimatization
Adventitious
shoots
Oilseed
Rooting
Tissue
culture
topic Acclimatization
Adventitious
shoots
Oilseed
Rooting
Tissue
culture
description Objective: It is necessary to improve biotech platforms based on in vitro cell tissue culture to support sacha inchi (Plukenetia volubilis L.) research programs and draw on the nutritional value of the high polyunsaturated fatty acid content of its oilseed. Here, we developed a rapid and efficient method for induction and direct in vitro shoot development for this species. Results: Shoots were generated from hypocotyl explants. The highest organogenic response was obtained in woody plant medium supplemented with 1 mg/L thidiazuron and 0.5 mg/L zeatin supplemented with L-glutamine, adenine hemisulfate, and L-arginine. Shoots obtained using this medium were transferred and subcultivated with different concentrations of indole-3-butyric acid and 1-naphthylacetic acid for rooting. For the first time, a histological analysis was performed supporting direct organogenic development in this species. The plantlets obtained were transferred ex vitro with a survival percentage of 80%. The genetic stability of the plants recovered was confirmed by randomly amplified polymorphic DNA analysis. All results indicate that it would be possible to stimulate direct shoot formation from hypocotyls to support the sustainable use of this species. © 2020 The Author(s).
publishDate 2020
dc.date.issued.none.fl_str_mv 2020-09-03
dc.date.available.none.fl_str_mv 2021-03-23T19:52:10Z
dc.date.accessioned.none.fl_str_mv 2021-03-23T19:52:10Z
dc.type.eng.fl_str_mv publishedVersion
info:eu-repo/semantics/publishedVersion
article
info:eu-repo/semantics/article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.local.spa.fl_str_mv Artículo
status_str publishedVersion
dc.identifier.none.fl_str_mv https://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=12169
dc.identifier.issn.none.fl_str_mv 17560500
dc.identifier.other.none.fl_str_mv PUBMED;32883361
SCOPUS;2-s2.0-85090319012
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10784/26758
dc.identifier.doi.none.fl_str_mv 10.1186/s13104-020-05257-1
url https://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=12169
http://hdl.handle.net/10784/26758
identifier_str_mv 17560500
PUBMED;32883361
SCOPUS;2-s2.0-85090319012
10.1186/s13104-020-05257-1
dc.language.iso.eng.fl_str_mv eng
language eng
dc.relation.uri.none.fl_str_mv https://www.scopus.com/inward/record.uri?eid=2-s2.0-85090319012&doi=10.1186%2fs13104-020-05257-1&partnerID=40&md5=17338532a6b5413b5901617efe0d4479
dc.rights.none.fl_str_mv https://v2.sherpa.ac.uk/id/publication/issn/1756-0500
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.local.spa.fl_str_mv Acceso abierto
rights_invalid_str_mv https://v2.sherpa.ac.uk/id/publication/issn/1756-0500
Acceso abierto
http://purl.org/coar/access_right/c_abf2
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central Ltd.
publisher.none.fl_str_mv BioMed Central Ltd.
dc.source.none.fl_str_mv BMC Research Notes
institution Universidad EAFIT
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bitstream.checksumAlgorithm.fl_str_mv MD5
repository.name.fl_str_mv Repositorio Institucional Universidad EAFIT
repository.mail.fl_str_mv repositorio@eafit.edu.co
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