Identification of Fusarium cf. Verticillioides as The Causal Agent of Pokka Boheng Disease in Sugarcane in the Department of Antioquia, Colombia

The cultivation of sugarcane represents an important part in the Colombian economy due to the diverse applications in industries like liquor, food, paper and biofuels. Sugarcane worldwide production is affected by the presence of phytopathogenic agents, mainly filamentous fungi such as Physalospora...

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Autores:
Giraldo Arias, Juliana
Celis Zapata, Santiago
Franco Sierra, Nicolás D
Arroyave Toro, Juan J
Jaramillo Mazo, Claudia
Correa Álvarez, Javier
Tipo de recurso:
Fecha de publicación:
2018
Institución:
Universidad EAFIT
Repositorio:
Repositorio EAFIT
Idioma:
eng
OAI Identifier:
oai:repository.eafit.edu.co:10784/14370
Acceso en línea:
http://hdl.handle.net/10784/14370
Palabra clave:
Sugarcane Pathogens
Fusarium Sp
Molecular Phytopathology
Phylogenetics
Patógenos De La Caña De Azúcar
Fusarium Sp
Fitopatología Molecular
Filogenia
Rights
License
Acceso abierto
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repository_id_str
dc.title.eng.fl_str_mv Identification of Fusarium cf. Verticillioides as The Causal Agent of Pokka Boheng Disease in Sugarcane in the Department of Antioquia, Colombia
dc.title.spa.fl_str_mv Identificación de Fusarium cf. verticillioides como agente causal de la enfermedad Pokka boheng en caña de azúcar en el departamento de Antioquia, Colombia
title Identification of Fusarium cf. Verticillioides as The Causal Agent of Pokka Boheng Disease in Sugarcane in the Department of Antioquia, Colombia
spellingShingle Identification of Fusarium cf. Verticillioides as The Causal Agent of Pokka Boheng Disease in Sugarcane in the Department of Antioquia, Colombia
Sugarcane Pathogens
Fusarium Sp
Molecular Phytopathology
Phylogenetics
Patógenos De La Caña De Azúcar
Fusarium Sp
Fitopatología Molecular
Filogenia
title_short Identification of Fusarium cf. Verticillioides as The Causal Agent of Pokka Boheng Disease in Sugarcane in the Department of Antioquia, Colombia
title_full Identification of Fusarium cf. Verticillioides as The Causal Agent of Pokka Boheng Disease in Sugarcane in the Department of Antioquia, Colombia
title_fullStr Identification of Fusarium cf. Verticillioides as The Causal Agent of Pokka Boheng Disease in Sugarcane in the Department of Antioquia, Colombia
title_full_unstemmed Identification of Fusarium cf. Verticillioides as The Causal Agent of Pokka Boheng Disease in Sugarcane in the Department of Antioquia, Colombia
title_sort Identification of Fusarium cf. Verticillioides as The Causal Agent of Pokka Boheng Disease in Sugarcane in the Department of Antioquia, Colombia
dc.creator.fl_str_mv Giraldo Arias, Juliana
Celis Zapata, Santiago
Franco Sierra, Nicolás D
Arroyave Toro, Juan J
Jaramillo Mazo, Claudia
Correa Álvarez, Javier
dc.contributor.author.spa.fl_str_mv Giraldo Arias, Juliana
Celis Zapata, Santiago
Franco Sierra, Nicolás D
Arroyave Toro, Juan J
Jaramillo Mazo, Claudia
Correa Álvarez, Javier
dc.contributor.affiliation.spa.fl_str_mv Universidad EAFIT
dc.subject.keyword.eng.fl_str_mv Sugarcane Pathogens
Fusarium Sp
Molecular Phytopathology
Phylogenetics
topic Sugarcane Pathogens
Fusarium Sp
Molecular Phytopathology
Phylogenetics
Patógenos De La Caña De Azúcar
Fusarium Sp
Fitopatología Molecular
Filogenia
dc.subject.keyword.spa.fl_str_mv Patógenos De La Caña De Azúcar
Fusarium Sp
Fitopatología Molecular
Filogenia
description The cultivation of sugarcane represents an important part in the Colombian economy due to the diverse applications in industries like liquor, food, paper and biofuels. Sugarcane worldwide production is affected by the presence of phytopathogenic agents, mainly filamentous fungi such as Physalospora tucumanensis (red rot disease) and Fusarium spp. To date in Colombia, Pokka boheng disease whose causal agent is the fungus Fusarium verticillioides, has not been reported, which is why it is necessary to identify appropriately this microorganism, responsible for losses in productivity and food contamination. In order to isolate and identify the infectious agent from symptomatic tissues, disinfection and culture in liquid and solid culture mediums were performed in malt extract (2%) and yeast extract (0.2%) both liquid and solid, for 7 days. After several replicates in agar plate, a purification was made along with a morphological characterization based on the shape and color of the mycelium, as well as the type of spores generated. Additionally, the genetic material was extracted and gene markers (ITS, Elongation Factor 1-α (EF) and β-Tubulin (Btub)) were amplified by PCR. Then, DNA sequencing was used to obtain the data to make a phylogenetic reconstruction by probabilistic methods (Maximum Likelihood and Bayesian Inference). The isolated strain, named as EA-FP0013 was located in the Fujikuroi complex group, with high probable identity to Fusarium verticillioides. Thus, early and species-specific identification of these fungal isolates by molecular methods may allow the timely diagnosis of emerging pathophysiological diseases of interest in the region, and thus propose the respective control strategies.
publishDate 2018
dc.date.issued.none.fl_str_mv 2018-11-23
dc.date.available.none.fl_str_mv 2019-11-22T16:02:24Z
dc.date.accessioned.none.fl_str_mv 2019-11-22T16:02:24Z
dc.date.none.fl_str_mv 2018-11-23
dc.type.eng.fl_str_mv article
info:eu-repo/semantics/article
publishedVersion
info:eu-repo/semantics/publishedVersion
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dc.identifier.issn.none.fl_str_mv 2256-4314
1794-9165
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10784/14370
dc.identifier.doi.none.fl_str_mv 10.17230/ingciencia.14.28.5
identifier_str_mv 2256-4314
1794-9165
10.17230/ingciencia.14.28.5
url http://hdl.handle.net/10784/14370
dc.language.iso.eng.fl_str_mv eng
language eng
dc.relation.ispartof.spa.fl_str_mv Ingeniería y Ciencia; Vol 14, No 28 (2018)
dc.relation.isversionof.none.fl_str_mv http://publicaciones.eafit.edu.co/index.php/ingciencia/article/view/5104
dc.relation.uri.none.fl_str_mv http://publicaciones.eafit.edu.co/index.php/ingciencia/article/view/5104
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dc.format.none.fl_str_mv application/pdf
dc.coverage.spatial.eng.fl_str_mv Medellín de: Lat: 06 15 00 N degrees minutes Lat: 6.2500 decimal degrees Long: 075 36 00 W degrees minutes Long: -75.6000 decimal degrees
dc.publisher.spa.fl_str_mv Universidad EAFIT
dc.source.none.fl_str_mv instname:Universidad EAFIT
reponame:Repositorio Institucional Universidad EAFIT
dc.source.spa.fl_str_mv Ingeniería y Ciencia; Vol 14, No 28 (2018)
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spelling Medellín de: Lat: 06 15 00 N degrees minutes Lat: 6.2500 decimal degrees Long: 075 36 00 W degrees minutes Long: -75.6000 decimal degrees2018-11-232019-11-22T16:02:24Z2018-11-232019-11-22T16:02:24Z2256-43141794-9165http://hdl.handle.net/10784/1437010.17230/ingciencia.14.28.5The cultivation of sugarcane represents an important part in the Colombian economy due to the diverse applications in industries like liquor, food, paper and biofuels. Sugarcane worldwide production is affected by the presence of phytopathogenic agents, mainly filamentous fungi such as Physalospora tucumanensis (red rot disease) and Fusarium spp. To date in Colombia, Pokka boheng disease whose causal agent is the fungus Fusarium verticillioides, has not been reported, which is why it is necessary to identify appropriately this microorganism, responsible for losses in productivity and food contamination. In order to isolate and identify the infectious agent from symptomatic tissues, disinfection and culture in liquid and solid culture mediums were performed in malt extract (2%) and yeast extract (0.2%) both liquid and solid, for 7 days. After several replicates in agar plate, a purification was made along with a morphological characterization based on the shape and color of the mycelium, as well as the type of spores generated. Additionally, the genetic material was extracted and gene markers (ITS, Elongation Factor 1-α (EF) and β-Tubulin (Btub)) were amplified by PCR. Then, DNA sequencing was used to obtain the data to make a phylogenetic reconstruction by probabilistic methods (Maximum Likelihood and Bayesian Inference). The isolated strain, named as EA-FP0013 was located in the Fujikuroi complex group, with high probable identity to Fusarium verticillioides. Thus, early and species-specific identification of these fungal isolates by molecular methods may allow the timely diagnosis of emerging pathophysiological diseases of interest in the region, and thus propose the respective control strategies.El cultivo de la caña de azúcar representa una parte importante en la economía de Colombia debido a las diversas aplicaciones en industrias como licor, alimentos, papel y biocombustibles. Su producción en todo el mundo se ve afectada por la presencia de agentes fitopatógenos, principalmente hongos filamentosos como Physalospora tucumanensis (agente causal de la enfermedad “Pudrición roja”) y Fusarium spp. Hasta la fecha, en Colombia, no se ha reportado la enfermedad de Pokka boheng en este cultivo cuyo agente causal es el hongo Fusarium verticillioides, por lo que es necesario identificar adecuadamente este microorganismo, responsable de las pérdidas en productividad y contaminación de los alimentos. Con el fin de aislar e identificar el agente infeccioso de los tejidos sintomáticos, se realizó la desinfección y posterior siembra de éstos en medios de cultivo extracto de malta (2 %) y extracto de levadura (0.2 %) tanto líquido como sólido durante 7 días. A partir de varias placas petri, se realizó la respectiva purificación y una caracterización morfológica basada en la forma y el color del micelio, así como el tipo de esporas generadas. Adicionalmente, el material genético se extrajo y se amplificaron los marcadores genéticos (ITS, factor de elongación 1-α (EF) y β-tubulina (Btub)) por PCR. Luego, se utilizó la secuenciación del ADN para obtener los datos para realizar una reconstrucción filogenética mediante métodos probabilísticos (máxima verosimilitud e inferencia bayesiana). La cepa aislada, nombrada como EA-FP0013 se localizó en el grupo del complejo Fujikuroi, con una alta identidad probable para Fusarium verticillioides. Por lo tanto, la identificación temprana y específica de especie de estos aislados fúngicos, utilizando métodos moleculares, puede permitir el diagnóstico oportuno de enfermedades fisiopatológicas emergentes de interés para la región, y así proponer las estrategias de control respectivas.application/pdfengUniversidad EAFITIngeniería y Ciencia; Vol 14, No 28 (2018)http://publicaciones.eafit.edu.co/index.php/ingciencia/article/view/5104http://publicaciones.eafit.edu.co/index.php/ingciencia/article/view/5104Copyright (c) 2018 Juliana Giraldo Arias, Santiago Celis Zapata, Nicolás D Franco Sierra, Juan J Arroyave Toro, Claudia Jaramillo Mazo, Javier Correa ÁlvarezAcceso abiertohttp://purl.org/coar/access_right/c_abf2instname:Universidad EAFITreponame:Repositorio Institucional Universidad EAFITIngeniería y Ciencia; Vol 14, No 28 (2018)Identification of Fusarium cf. Verticillioides as The Causal Agent of Pokka Boheng Disease in Sugarcane in the Department of Antioquia, ColombiaIdentificación de Fusarium cf. verticillioides como agente causal de la enfermedad Pokka boheng en caña de azúcar en el departamento de Antioquia, Colombiaarticleinfo:eu-repo/semantics/articlepublishedVersioninfo:eu-repo/semantics/publishedVersionArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Sugarcane PathogensFusarium SpMolecular PhytopathologyPhylogeneticsPatógenos De La Caña De AzúcarFusarium SpFitopatología MolecularFilogeniaGiraldo Arias, JulianaCelis Zapata, SantiagoFranco Sierra, Nicolás DArroyave Toro, Juan JJaramillo Mazo, ClaudiaCorrea Álvarez, JavierUniversidad EAFITIngeniería y Ciencia1428113134ing.cienc.THUMBNAILminaitura-ig_Mesa de trabajo 1.jpgminaitura-ig_Mesa de trabajo 1.jpgimage/jpeg265796https://repository.eafit.edu.co/bitstreams/f0d0a99f-176c-46ae-a72f-dbc9fc8a3d71/downloadda9b21a5c7e00c7f1127cef8e97035e0MD51ORIGINALdocument (4).pdfdocument (4).pdfTexto completo PDFapplication/pdf2951812https://repository.eafit.edu.co/bitstreams/dcd18628-7d63-4c6a-b572-b76e9e596813/download1f4a2f6126dbedf4341dad1a66177075MD52articulo.htmlarticulo.htmlTexto completo HTMLtext/html374https://repository.eafit.edu.co/bitstreams/336716fd-ffad-4056-b2e1-114cace4c428/downloaddbe14f2bd165f2fadb5bf2e2bec2ec8fMD5310784/14370oai:repository.eafit.edu.co:10784/143702022-04-27 09:59:43.988open.accesshttps://repository.eafit.edu.coRepositorio Institucional Universidad EAFITrepositorio@eafit.edu.co