Construction of probe of the plant growth-promoting bacteria Bacillus subtilis useful for fluorescence in situ hybridization

Strains of Bacillus subtilis are plant growth-promoting bacteria (PGPB) of many crops and are used as inoculants. PGPB colonization is an important trait for success of a PGPB on plants. A specific probe, based on the 16 s rRNA of Bacillus subtilis, was designed and evaluated to distinguishing, by f...

Full description

Autores:
Posada, L.F.
Alvarez, J.C.
Hu, C.-H.
de-Bashan, L.E.
Bashan, Y.
Tipo de recurso:
Fecha de publicación:
2016
Institución:
Universidad EAFIT
Repositorio:
Repositorio EAFIT
Idioma:
eng
OAI Identifier:
oai:repository.eafit.edu.co:10784/26728
Acceso en línea:
https://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=4283
http://hdl.handle.net/10784/26728
Palabra clave:
Bacillus
subtilis
Specific
probe
Fluorescence
in
situ
hybridization
B
amyloliquefaciens
Rights
License
https://v2.sherpa.ac.uk/id/publication/issn/0167-7012
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network_name_str Repositorio EAFIT
repository_id_str
spelling 2021-03-23T19:52:07Z2016-09-012021-03-23T19:52:07Zhttps://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=42830167701218728359WOS;000382346300024PUBMED;27263830SCOPUS;2-s2.0-84979498465http://hdl.handle.net/10784/2672810.1016/j.mimet.2016.05.029Strains of Bacillus subtilis are plant growth-promoting bacteria (PGPB) of many crops and are used as inoculants. PGPB colonization is an important trait for success of a PGPB on plants. A specific probe, based on the 16 s rRNA of Bacillus subtilis, was designed and evaluated to distinguishing, by fluorescence in situ hybridization (FISH), between this species and the closely related Bacillus amyloliquefaciens. The selected target for the probe was between nucleotides 465 and 483 of the gene, where three different nucleotides can be identified. The designed probe successfully hybridized with several strains of Bacillus subtilis, but failed to hybridize not only with B. amyloliquefaciens, but also with other strains such as Bacillus altitudinis, Bacillus cereus, Bacillus gibsonii, Bacillus megaterium, Bacillus pumilus; and with the external phylogenetic strains Azospirillum brasilense Cd, Micrococcus sp. and Paenibacillus sp. The results showed the specificity of this molecular probe for B. subtilis. (C) 2016 Elsevier B.V. All rights reserved.application/pdfengElsevierhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84979498465&doi=10.1016%2fj.mimet.2016.05.029&partnerID=40&md5=c73e5fb018c3e26ae209436afd70161ehttps://v2.sherpa.ac.uk/id/publication/issn/0167-7012Acceso abiertohttp://purl.org/coar/access_right/c_abf2JOURNAL OF MICROBIOLOGICAL METHODSBacillussubtilisSpecificprobeFluorescenceinsituhybridizationBamyloliquefaciensConstruction of probe of the plant growth-promoting bacteria Bacillus subtilis useful for fluorescence in situ hybridizationpublishedVersioninfo:eu-repo/semantics/publishedVersionarticleinfo:eu-repo/semantics/articleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Universidad EAFIT. Departamento de CienciasPosada, L.F.Alvarez, J.C.Hu, C.-H.de-Bashan, L.E.Bashan, Y.Biodiversidad, Evolución y ConservaciónJOURNAL OF MICROBIOLOGICAL METHODSORIGINAL1-s2.0-S0167701216301269-main.pdf1-s2.0-S0167701216301269-main.pdfTexto completoapplication/pdf554155https://repository.eafit.edu.co/bitstreams/2c2f1ac8-5ce8-47a5-8d3b-9951ec2c64b3/download4c41e151d6355b53c6787deec0b695e8MD5110784/26728oai:repository.eafit.edu.co:10784/267282022-04-27 15:49:56.747open.accesshttps://repository.eafit.edu.coRepositorio Institucional Universidad EAFITrepositorio@eafit.edu.co
dc.title.eng.fl_str_mv Construction of probe of the plant growth-promoting bacteria Bacillus subtilis useful for fluorescence in situ hybridization
title Construction of probe of the plant growth-promoting bacteria Bacillus subtilis useful for fluorescence in situ hybridization
spellingShingle Construction of probe of the plant growth-promoting bacteria Bacillus subtilis useful for fluorescence in situ hybridization
Bacillus
subtilis
Specific
probe
Fluorescence
in
situ
hybridization
B
amyloliquefaciens
title_short Construction of probe of the plant growth-promoting bacteria Bacillus subtilis useful for fluorescence in situ hybridization
title_full Construction of probe of the plant growth-promoting bacteria Bacillus subtilis useful for fluorescence in situ hybridization
title_fullStr Construction of probe of the plant growth-promoting bacteria Bacillus subtilis useful for fluorescence in situ hybridization
title_full_unstemmed Construction of probe of the plant growth-promoting bacteria Bacillus subtilis useful for fluorescence in situ hybridization
title_sort Construction of probe of the plant growth-promoting bacteria Bacillus subtilis useful for fluorescence in situ hybridization
dc.creator.fl_str_mv Posada, L.F.
Alvarez, J.C.
Hu, C.-H.
de-Bashan, L.E.
Bashan, Y.
dc.contributor.department.spa.fl_str_mv Universidad EAFIT. Departamento de Ciencias
dc.contributor.author.none.fl_str_mv Posada, L.F.
Alvarez, J.C.
Hu, C.-H.
de-Bashan, L.E.
Bashan, Y.
dc.contributor.researchgroup.spa.fl_str_mv Biodiversidad, Evolución y Conservación
dc.subject.eng.fl_str_mv Bacillus
subtilis
Specific
probe
Fluorescence
in
situ
hybridization
B
amyloliquefaciens
topic Bacillus
subtilis
Specific
probe
Fluorescence
in
situ
hybridization
B
amyloliquefaciens
description Strains of Bacillus subtilis are plant growth-promoting bacteria (PGPB) of many crops and are used as inoculants. PGPB colonization is an important trait for success of a PGPB on plants. A specific probe, based on the 16 s rRNA of Bacillus subtilis, was designed and evaluated to distinguishing, by fluorescence in situ hybridization (FISH), between this species and the closely related Bacillus amyloliquefaciens. The selected target for the probe was between nucleotides 465 and 483 of the gene, where three different nucleotides can be identified. The designed probe successfully hybridized with several strains of Bacillus subtilis, but failed to hybridize not only with B. amyloliquefaciens, but also with other strains such as Bacillus altitudinis, Bacillus cereus, Bacillus gibsonii, Bacillus megaterium, Bacillus pumilus; and with the external phylogenetic strains Azospirillum brasilense Cd, Micrococcus sp. and Paenibacillus sp. The results showed the specificity of this molecular probe for B. subtilis. (C) 2016 Elsevier B.V. All rights reserved.
publishDate 2016
dc.date.issued.none.fl_str_mv 2016-09-01
dc.date.available.none.fl_str_mv 2021-03-23T19:52:07Z
dc.date.accessioned.none.fl_str_mv 2021-03-23T19:52:07Z
dc.type.eng.fl_str_mv publishedVersion
info:eu-repo/semantics/publishedVersion
article
info:eu-repo/semantics/article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.local.spa.fl_str_mv Artículo
status_str publishedVersion
dc.identifier.none.fl_str_mv https://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=4283
dc.identifier.issn.none.fl_str_mv 01677012
18728359
dc.identifier.other.none.fl_str_mv WOS;000382346300024
PUBMED;27263830
SCOPUS;2-s2.0-84979498465
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10784/26728
dc.identifier.doi.none.fl_str_mv 10.1016/j.mimet.2016.05.029
url https://eafit.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=4283
http://hdl.handle.net/10784/26728
identifier_str_mv 01677012
18728359
WOS;000382346300024
PUBMED;27263830
SCOPUS;2-s2.0-84979498465
10.1016/j.mimet.2016.05.029
dc.language.iso.eng.fl_str_mv eng
language eng
dc.relation.uri.none.fl_str_mv https://www.scopus.com/inward/record.uri?eid=2-s2.0-84979498465&doi=10.1016%2fj.mimet.2016.05.029&partnerID=40&md5=c73e5fb018c3e26ae209436afd70161e
dc.rights.none.fl_str_mv https://v2.sherpa.ac.uk/id/publication/issn/0167-7012
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.local.spa.fl_str_mv Acceso abierto
rights_invalid_str_mv https://v2.sherpa.ac.uk/id/publication/issn/0167-7012
Acceso abierto
http://purl.org/coar/access_right/c_abf2
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv JOURNAL OF MICROBIOLOGICAL METHODS
institution Universidad EAFIT
bitstream.url.fl_str_mv https://repository.eafit.edu.co/bitstreams/2c2f1ac8-5ce8-47a5-8d3b-9951ec2c64b3/download
bitstream.checksum.fl_str_mv 4c41e151d6355b53c6787deec0b695e8
bitstream.checksumAlgorithm.fl_str_mv MD5
repository.name.fl_str_mv Repositorio Institucional Universidad EAFIT
repository.mail.fl_str_mv repositorio@eafit.edu.co
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