Mechanotransduction-dependent changes in the contributions of actin isoforms to the auditory stereocilia cytoskeleton
56 páginas
- Autores:
-
Macias Palacio, Sara Legny
- Tipo de recurso:
- Fecha de publicación:
- 2021
- Institución:
- Universidad EIA .
- Repositorio:
- Repositorio EIA .
- Idioma:
- spa
- OAI Identifier:
- oai:repository.eia.edu.co:11190/4188
- Acceso en línea:
- https://repository.eia.edu.co/handle/11190/4188
- Palabra clave:
- Hair cells
Stereocilia
Actin isoforms
Mechanotransduction channels
- Rights
- openAccess
- License
- Derechos Reservados - Universidad EIA, 2021
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| dc.title.spa.fl_str_mv |
Mechanotransduction-dependent changes in the contributions of actin isoforms to the auditory stereocilia cytoskeleton |
| title |
Mechanotransduction-dependent changes in the contributions of actin isoforms to the auditory stereocilia cytoskeleton |
| spellingShingle |
Mechanotransduction-dependent changes in the contributions of actin isoforms to the auditory stereocilia cytoskeleton Hair cells Stereocilia Actin isoforms Mechanotransduction channels |
| title_short |
Mechanotransduction-dependent changes in the contributions of actin isoforms to the auditory stereocilia cytoskeleton |
| title_full |
Mechanotransduction-dependent changes in the contributions of actin isoforms to the auditory stereocilia cytoskeleton |
| title_fullStr |
Mechanotransduction-dependent changes in the contributions of actin isoforms to the auditory stereocilia cytoskeleton |
| title_full_unstemmed |
Mechanotransduction-dependent changes in the contributions of actin isoforms to the auditory stereocilia cytoskeleton |
| title_sort |
Mechanotransduction-dependent changes in the contributions of actin isoforms to the auditory stereocilia cytoskeleton |
| dc.creator.fl_str_mv |
Macias Palacio, Sara Legny |
| dc.contributor.advisor.none.fl_str_mv |
Velez Ortega, A. Catalina |
| dc.contributor.author.none.fl_str_mv |
Macias Palacio, Sara Legny |
| dc.subject.proposal.eng.fl_str_mv |
Hair cells Stereocilia Actin isoforms Mechanotransduction channels |
| topic |
Hair cells Stereocilia Actin isoforms Mechanotransduction channels |
| description |
56 páginas |
| publishDate |
2021 |
| dc.date.issued.none.fl_str_mv |
2021 |
| dc.date.accessioned.none.fl_str_mv |
2022-01-20T22:22:59Z |
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2022-01-20T22:22:59Z |
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Trabajo de grado - Pregrado |
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Macias Palacio, S. L. (2021). Mechanotransduction-dependent changes in the contributions of actin isoforms to the auditory stereocilia cytoskeleton [tesis de pregrado, Universidad EIA]. Repositorio Institucional Universidad EIA. https://repository.eia.edu.co/handle/11190/4188 |
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Macias Palacio, S. L. (2021). Mechanotransduction-dependent changes in the contributions of actin isoforms to the auditory stereocilia cytoskeleton [tesis de pregrado, Universidad EIA]. Repositorio Institucional Universidad EIA. https://repository.eia.edu.co/handle/11190/4188 |
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spa |
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spa |
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Derechos Reservados - Universidad EIA, 2021 |
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https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) |
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Derechos Reservados - Universidad EIA, 2021 https://creativecommons.org/licenses/by-nc-nd/4.0/ Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) http://purl.org/coar/access_right/c_abf2 |
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Universidad EIA |
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Ingeniería Biomédica |
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Escuela de Ciencias de la Vida |
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Envigado (Antioquia, Colombia) |
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Universidad EIA . |
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Velez Ortega, A. Catalinad64eef19b4438bda8a8961cb3a292fe4600Macias Palacio, Sara Legnye440e25e26be62c8049f970c864cd4532022-01-20T22:22:59Z2022-01-20T22:22:59Z2021https://repository.eia.edu.co/handle/11190/4188Macias Palacio, S. L. (2021). Mechanotransduction-dependent changes in the contributions of actin isoforms to the auditory stereocilia cytoskeleton [tesis de pregrado, Universidad EIA]. Repositorio Institucional Universidad EIA. https://repository.eia.edu.co/handle/11190/418856 páginasABSTRACT: Stereocilia, mechanosensitive projections on top of the inner ear hair cells, are made of filaments from both cytoplasmatic actin isoforms: β and γ- (Furness et al., 2005; Perrin et al., 2010). The absence of either of these isoforms results in the normal growth of stereocilia but they eventually degenerate (Belyantseva et al., 2009; Perrin et al., 2010). Moreover, a decrease in the resting influx of Ca2+ through the mechanotransduction (MET) channels, disrupts the stability of the stereocilia cytoskeleton, leading to thinning and shrinking of stereocilia (Vélez-Ortega et al., 2017). When bound to Ca2+, β- actin has faster polymerization and depolymerization kinetics than γ- actin (Bergeron et al., 2010) so changes in Ca2+ influx to the hair cell through MET channels might affect both isoforms in a different way. Therefore, the existence of MET-dependent changes in the distribution of β- and γ- actin along stereocilia lengths in mouse auditory hair cells was evaluated. Organ of Corti explants from wildtype mice were isolated at early postnatal days and cultured in control conditions or in the presence of the MET channel blocker tubocurarine (60 µM). Explants were fixed when freshly isolated or at specific time points after MET channel blockage. Depending on the stage of the project, samples were either immunostained with fluorescently-labeled antibodies against β- and γ-actin isoforms and imaged via confocal microscopy, or prepared for scanning electron microscopy (SEM) imaging. Obtained images from confocal microscopy were processed and measured using the created algorithm. It was found that the ratios of γ- to β- actin along the length of stereocilia were not affected in culture conditions up to 48 hours. Evidence was provided on the thinning and shortening of second and third row of stereocilia after the incubation with tubocurarine. Nevertheless, according to the presented data on the fluorescence quantification, no changes in the ratio of β- and γ- actin were found during these MET-dependent cytoskeleton rearrangements. Therefore, it can be affirmed that Ca2+-dependent differences in polymerization and depolymerization rates between β- and γ- actin are not the main mechanism driving stereocilia cytoskeleton rearrangements upon changes in intracellular Ca2+ concentrations in mammalian auditory hair cells, since the proportions of both actin isoforms were maintained in the stereocilia cytoskeleton during this process.PregradoIngeniero(a) Biomédico(a)application/pdfspaUniversidad EIAIngeniería BiomédicaEscuela de Ciencias de la VidaEnvigado (Antioquia, Colombia)Derechos Reservados - Universidad EIA, 2021https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)http://purl.org/coar/access_right/c_abf2Mechanotransduction-dependent changes in the contributions of actin isoforms to the auditory stereocilia cytoskeletonTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/publishedVersionTexthttps://purl.org/redcol/resource_type/TPhttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_7a1fHair cellsStereociliaActin isoformsMechanotransduction channelsPublicationLICENSElicense.txtlicense.txttext/plain; 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