Formation of three-dimensional cell culture of colorectal carcinoma cells for possible applications in alternative cancer treatment in vitro

63 páginas

Autores:
Parias Carreño, Andrés Felipe
Tipo de recurso:
Trabajo de grado de pregrado
Fecha de publicación:
2023
Institución:
Universidad EIA .
Repositorio:
Repositorio EIA .
Idioma:
eng
OAI Identifier:
oai:repository.eia.edu.co:11190/6325
Acceso en línea:
https://repository.eia.edu.co/handle/11190/6325
Palabra clave:
Caco-2
Spheroids
FBS
In vitro
3D culture
Rights
openAccess
License
Derechos Reservados - Universidad EIA, 2023
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dc.title.eng.fl_str_mv Formation of three-dimensional cell culture of colorectal carcinoma cells for possible applications in alternative cancer treatment in vitro
title Formation of three-dimensional cell culture of colorectal carcinoma cells for possible applications in alternative cancer treatment in vitro
spellingShingle Formation of three-dimensional cell culture of colorectal carcinoma cells for possible applications in alternative cancer treatment in vitro
Caco-2
Spheroids
FBS
In vitro
3D culture
title_short Formation of three-dimensional cell culture of colorectal carcinoma cells for possible applications in alternative cancer treatment in vitro
title_full Formation of three-dimensional cell culture of colorectal carcinoma cells for possible applications in alternative cancer treatment in vitro
title_fullStr Formation of three-dimensional cell culture of colorectal carcinoma cells for possible applications in alternative cancer treatment in vitro
title_full_unstemmed Formation of three-dimensional cell culture of colorectal carcinoma cells for possible applications in alternative cancer treatment in vitro
title_sort Formation of three-dimensional cell culture of colorectal carcinoma cells for possible applications in alternative cancer treatment in vitro
dc.creator.fl_str_mv Parias Carreño, Andrés Felipe
dc.contributor.advisor.none.fl_str_mv Toro, Lenka
dc.contributor.author.none.fl_str_mv Parias Carreño, Andrés Felipe
dc.subject.proposal.eng.fl_str_mv Caco-2
Spheroids
FBS
In vitro
3D culture
topic Caco-2
Spheroids
FBS
In vitro
3D culture
description 63 páginas
publishDate 2023
dc.date.issued.none.fl_str_mv 2023
dc.date.accessioned.none.fl_str_mv 2024-01-22T19:14:28Z
dc.date.available.none.fl_str_mv 2024-01-22T19:14:28Z
dc.type.none.fl_str_mv Trabajo de grado - Pregrado
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dc.identifier.uri.none.fl_str_mv https://repository.eia.edu.co/handle/11190/6325
url https://repository.eia.edu.co/handle/11190/6325
dc.language.iso.none.fl_str_mv eng
language eng
dc.rights.spa.fl_str_mv Derechos Reservados - Universidad EIA, 2023
dc.rights.license.none.fl_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
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eu_rights_str_mv openAccess
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad EIA
dc.publisher.program.none.fl_str_mv Ingeniería Biomédica
dc.publisher.faculty.none.fl_str_mv Escuela de Ciencias de la Vida
dc.publisher.place.none.fl_str_mv Envigado (Antioquia, Colombia)
publisher.none.fl_str_mv Universidad EIA
institution Universidad EIA .
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spelling Toro, LenkaParias Carreño, Andrés Felipe2024-01-22T19:14:28Z2024-01-22T19:14:28Z2023https://repository.eia.edu.co/handle/11190/632563 páginasABSTRACT: according to the Global Cancer Observatory, colorectal cancer (CRC) is the third most recurrent cancer worldwide and in Colombia. For 2022, the American Cancer Society previewed that it will be the cause of death of 52,580 persons. Current conventional treatments can generate adverse effects and there is a need for new alternative methods to fight this disease. In order to test these new alternative methods, there is a need for a model that correctly represents the human tissue microenvironment to determine the possible effect of these new treatments. 3D models have the ability to form cell-to-cell interactions, mimic in vivo inflammatory response, develop drug resistance and allows the possibility for evaluating the treatment targeting capabilities. In this work the use of 3D cancer cell cultures is proposed as a possible model for the evaluation of novel alternative methods to fight colorectal cancer. The main objective of this project is to evaluate the ability of cancer cell line Caco-2 to form a 3D cell culture for possible applications in alternative colorectal cancer treatment. For the project execution, the culture conditions for 2D cell cultures were established based on a literature review in order to determine the 3D cell culture conditions. For the formation of the 2D culture and subcultures different experimental setups were tested to identify the best conditions for the 2D cell culture. The 3D cell culture was formed using the hanging drops method in droplets of 25 µL to obtain Caco-2 derived spheroids. The spheroids growth formed with different cell concentrations was observed to determine which conditions provided the best results for the formation of the spheroids. After the spheroids were formed the viability was evaluated by measuring the metabolic activity of the cells in the spheroids and the response to fluorescent dyes. To evaluate the proliferation a measure of the diameter of a single cell inside the droplet was recorded in day 1 and compared to the diameter of the spheroids formed. Morphology was observed under Scanning Electron Microscopy and all the results were evaluated using statistical analysis to determine the significance. As a result, Caco-2 derived spheroids were formed in 6 days under different cell concentrations with average diameters ranged between 70 and 80 µm in complete culture media supplemented with 20% FBS and 0.1% gentamycin with no additional supplements.PregradoIngeniero(a) Biomédico(a)application/pdfengUniversidad EIAIngeniería BiomédicaEscuela de Ciencias de la VidaEnvigado (Antioquia, Colombia)Derechos Reservados - Universidad EIA, 2023Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Formation of three-dimensional cell culture of colorectal carcinoma cells for possible applications in alternative cancer treatment in vitroTrabajo de grado - Pregradohttp://purl.org/coar/resource_type/c_7a1finfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/publishedVersionTexthttp://purl.org/redcol/resource_type/TPhttp://purl.org/coar/version/c_970fb48d4fbd8a85Caco-2SpheroidsFBSIn vitro3D culturePublicationLICENSElicense.txtlicense.txttext/plain; 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