Engineering antibody fragments: replicating the immune system and beyond
Since genetic engineering of humanized murine monoclonal antibodies was fi rst demonstrated over two decades ago, antibody engineering technologies have evolved based upon an increasing understanding of the mechanisms involved in antibody generation in vivo, and a constant search for alternative rou...
- Autores:
-
García-Quiroz, F. (Felipe)
Sinclair, M. (Michael)
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2010
- Institución:
- Universidad EIA .
- Repositorio:
- Repositorio EIA .
- Idioma:
- eng
- OAI Identifier:
- oai:repository.eia.edu.co:11190/484
- Acceso en línea:
- https://repository.eia.edu.co/handle/11190/484
- Palabra clave:
- RBI00073
TECNOLOGÍAS PARA LA SALUD
TECHNOLOGY IN HEALTH
ANTIBODIES - PHYSIOLOGY
ANTICUERPOS - FISIOLOGÍA
ANTIBODY ENGINEERING
PHASE DISPLAY
CELL DISPLAY
RIBOSOME DISPLAY
ANTIBODY HUMANIZATION
INGENIERIA DE ANTICUERPOS
PRESENTACION EN FAGOS
PRESENTACION EN CELULAS
PRESENTACION EN RIBOSOMAS
HUMANIZACION DE ANTICUERPOS
- Rights
- openAccess
- License
- Derechos Reservados - Universidad EIA, 2020
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| dc.title.spa.fl_str_mv |
Engineering antibody fragments: replicating the immune system and beyond |
| dc.title.alternative.spa.fl_str_mv |
La ingeniería de fragmentos de anticuerpos: imitando y expandiendo el sistema inmune |
| title |
Engineering antibody fragments: replicating the immune system and beyond |
| spellingShingle |
Engineering antibody fragments: replicating the immune system and beyond RBI00073 TECNOLOGÍAS PARA LA SALUD TECHNOLOGY IN HEALTH ANTIBODIES - PHYSIOLOGY ANTICUERPOS - FISIOLOGÍA ANTIBODY ENGINEERING PHASE DISPLAY CELL DISPLAY RIBOSOME DISPLAY ANTIBODY HUMANIZATION INGENIERIA DE ANTICUERPOS PRESENTACION EN FAGOS PRESENTACION EN CELULAS PRESENTACION EN RIBOSOMAS HUMANIZACION DE ANTICUERPOS |
| title_short |
Engineering antibody fragments: replicating the immune system and beyond |
| title_full |
Engineering antibody fragments: replicating the immune system and beyond |
| title_fullStr |
Engineering antibody fragments: replicating the immune system and beyond |
| title_full_unstemmed |
Engineering antibody fragments: replicating the immune system and beyond |
| title_sort |
Engineering antibody fragments: replicating the immune system and beyond |
| dc.creator.fl_str_mv |
García-Quiroz, F. (Felipe) Sinclair, M. (Michael) |
| dc.contributor.author.spa.fl_str_mv |
García-Quiroz, F. (Felipe) Sinclair, M. (Michael) |
| dc.subject.lcsh.spa.fl_str_mv |
RBI00073 |
| topic |
RBI00073 TECNOLOGÍAS PARA LA SALUD TECHNOLOGY IN HEALTH ANTIBODIES - PHYSIOLOGY ANTICUERPOS - FISIOLOGÍA ANTIBODY ENGINEERING PHASE DISPLAY CELL DISPLAY RIBOSOME DISPLAY ANTIBODY HUMANIZATION INGENIERIA DE ANTICUERPOS PRESENTACION EN FAGOS PRESENTACION EN CELULAS PRESENTACION EN RIBOSOMAS HUMANIZACION DE ANTICUERPOS |
| dc.subject.eia.spa.fl_str_mv |
TECNOLOGÍAS PARA LA SALUD TECHNOLOGY IN HEALTH |
| dc.subject.eurovoc.spa.fl_str_mv |
ANTIBODIES - PHYSIOLOGY ANTICUERPOS - FISIOLOGÍA |
| dc.subject.keywords.spa.fl_str_mv |
ANTIBODY ENGINEERING PHASE DISPLAY CELL DISPLAY RIBOSOME DISPLAY ANTIBODY HUMANIZATION INGENIERIA DE ANTICUERPOS PRESENTACION EN FAGOS PRESENTACION EN CELULAS PRESENTACION EN RIBOSOMAS HUMANIZACION DE ANTICUERPOS |
| description |
Since genetic engineering of humanized murine monoclonal antibodies was fi rst demonstrated over two decades ago, antibody engineering technologies have evolved based upon an increasing understanding of the mechanisms involved in antibody generation in vivo, and a constant search for alternative routes to evolve and exploit the characteristics of antibodies. As a result, antibody engineers have devised innovative strategies for the rapid evolution and selection of antibodies and novel antibody designs (i.e., antibody fragments). Phage display, cell display and ribosome display technologies, which comprise the core of the currently available technologies for the discovery and preparation of such antibodies, are reviewed herein. This article intends to communicate the state-of-the-art technology available for the engineering of antibodies to a general readership interested in this important fi eld. Therefore, important immunology concepts are introduced before detailed descriptions of the three antibody engineering technologies are presented in later sections. A comparison of these methodologies suggests that despite the predominance of phage display for the engineering of antibody fragments in the past 20 years, cell display and ribosome display will likely gain importance in the selection and discovery of the antibody fragments in the future. Finally, these technologies are likely to play an important role in the production of the next generation of antibody-based therapeutics. |
| publishDate |
2010 |
| dc.date.created.spa.fl_str_mv |
2010-06 |
| dc.date.submitted.spa.fl_str_mv |
2010-05-25 |
| dc.date.accepted.spa.fl_str_mv |
2010-06-29 |
| dc.date.accessioned.spa.fl_str_mv |
2014-04-24T22:14:27Z |
| dc.date.available.spa.fl_str_mv |
2014-04-24T22:14:27Z |
| dc.date.issued.spa.fl_str_mv |
2014-04-24 |
| dc.type.spa.fl_str_mv |
Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/resource_type/c_6501 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
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https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_6501 |
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publishedVersion |
| dc.identifier.issn.spa.fl_str_mv |
ISSN 19099762 |
| dc.identifier.uri.spa.fl_str_mv |
https://repository.eia.edu.co/handle/11190/484 |
| dc.identifier.bibliographiccitation.spa.fl_str_mv |
García-Quiroz, F. and Sinclair, M. Engineering antibody fragments: replicating the immune system and beyond, Revista Ingeniería Biomédica, 3 (7), 39-51. doi: http://repository. eia.edu.co/handle/11190/484 |
| identifier_str_mv |
ISSN 19099762 García-Quiroz, F. and Sinclair, M. Engineering antibody fragments: replicating the immune system and beyond, Revista Ingeniería Biomédica, 3 (7), 39-51. doi: http://repository. eia.edu.co/handle/11190/484 |
| url |
https://repository.eia.edu.co/handle/11190/484 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.references.spa.fl_str_mv |
Carter P.J. Potent antibody therapeutics by design. Nature reviews. Immunology, 6:343-357, 2006. Filpula D. Antibody engineering and modifi cation technologies. Biomolecular engineering, 24:201-215, 2007. Salfeld J.G. Isotype selection in antibody engineering. Nature biotechnology, 25:1369-1372, 2007. |
| dc.rights.spa.fl_str_mv |
Derechos Reservados - Universidad EIA, 2020 |
| dc.rights.uri.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc/4.0/ |
| dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
| dc.rights.creativecommons.spa.fl_str_mv |
Atribución-NoComercial |
| dc.rights.coar.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
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Derechos Reservados - Universidad EIA, 2020 https://creativecommons.org/licenses/by-nc/4.0/ Atribución-NoComercial http://purl.org/coar/access_right/c_abf2 |
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openAccess |
| dc.format.extent.spa.fl_str_mv |
13 p. |
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application/pdf |
| dc.publisher.department.spa.fl_str_mv |
Biomédica, Mecatrónica y Mecánica |
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Escuela de Ingeniería de Antioquia EIA Universidad CES |
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Universidad EIA . |
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García-Quiroz, F. (Felipe)e05020b21ff871eef7f5d52328ebd5ac-1Sinclair, M. (Michael)dff0f493089daaaf397ecaee4acbd284-1felipe.garcia@duke.edu2014-04-24T22:14:27Z2014-04-24T22:14:27Z2010-062014-04-242010-05-252010-06-29ISSN 19099762https://repository.eia.edu.co/handle/11190/484García-Quiroz, F. and Sinclair, M. Engineering antibody fragments: replicating the immune system and beyond, Revista Ingeniería Biomédica, 3 (7), 39-51. doi: http://repository. eia.edu.co/handle/11190/484Since genetic engineering of humanized murine monoclonal antibodies was fi rst demonstrated over two decades ago, antibody engineering technologies have evolved based upon an increasing understanding of the mechanisms involved in antibody generation in vivo, and a constant search for alternative routes to evolve and exploit the characteristics of antibodies. As a result, antibody engineers have devised innovative strategies for the rapid evolution and selection of antibodies and novel antibody designs (i.e., antibody fragments). Phage display, cell display and ribosome display technologies, which comprise the core of the currently available technologies for the discovery and preparation of such antibodies, are reviewed herein. This article intends to communicate the state-of-the-art technology available for the engineering of antibodies to a general readership interested in this important fi eld. Therefore, important immunology concepts are introduced before detailed descriptions of the three antibody engineering technologies are presented in later sections. A comparison of these methodologies suggests that despite the predominance of phage display for the engineering of antibody fragments in the past 20 years, cell display and ribosome display will likely gain importance in the selection and discovery of the antibody fragments in the future. Finally, these technologies are likely to play an important role in the production of the next generation of antibody-based therapeutics.Las tecnologías para la ingeniería de anticuerpos han evolucionado durante las últimas dos décadas, desde la demostración de la posibilidad de humanizar anticuerpos monoclonales de ratón mediante ingeniería genética, apoyadas en el creciente entendimiento de los mecanismos involucrados en la generación de anticuerpos in vivo, y en una búsqueda constante de rutas alternativas para evolucionar y explotar sus características. Es así como los ingenieros de anticuerpos han desarrollado estrategias innovadoras para la evolución y selección de anticuerpos y de novedosos diseños de anticuerpos conocidos como fragmentos de anticuerpos. Esta revisión se enfoca en tres tecnologías que comprenden el núcleo de las tecnologías actualmente disponibles para el descubrimiento y preparación de tales anticuerpos: la presentación en fagos, la presentación en células, y la presentación en ribosomas. Este artículo busca presentar el estado del arte de estas tecnologías a un grupo general de lectores interesados en este campo, por lo que inicialmente se introducen importantes conceptos de inmunología requeridos para comprender en detalle las tecnologías discutidas. Una comparación de estas metodologías para la ingeniería de anticuerpos sugiere que a pesar del dominio de las tecnologías basadas en la presentación en fagos durante los últimos 20 años, en los próximos años la presentación en células y la presentación en ribosomas probablemente ganarán importancia para la selección y descubrimiento de fragmentos de anticuerpos. Finalmente, es probable que estas tecnologías jueguen un papel importante en la producción de la siguiente generación de terapéuticos basados en anticuerpos.13 p.application/pdfengDerechos Reservados - Universidad EIA, 2020https://creativecommons.org/licenses/by-nc/4.0/El autor de la obra, actuando en nombre propio, hace entrega del ejemplar respectivo y de sus anexos en formato digital o electrónico y autoriza a la ESCUELA DE INGENIERIA DE ANTIOQUIA, para que en los términos establecidos en la Ley 23 de 1982, Ley 44 de 1993, Decisión andina 351 de 1993, Decreto 460 de 1995, y demás normas generales sobre la materia, utilice y use por cualquier medio conocido o por conocer, los derechos patrimoniales de reproducción, comunicación pública, transformación y distribución de la obra objeto del presente documento. PARÁGRAFO: La presente autorización se hace extensiva no sólo a las dependencias y derechos de uso sobre la obra en formato o soporte material, sino también para formato virtual, electrónico, digital, y en red, internet, extranet, intranet, etc., y en general en cualquier formato conocido o por conocer. EL AUTOR, manifiesta que la obra objeto de la presente autorización es original y la realiza sin violar o usurpar derechos de autor de terceros, por lo tanto la obra es de exclusiva autoría y tiene la titularidad sobre la misma. PARÁGRAFO: En caso de presentarse cualquier reclamación o acción por parte de un tercero en cuanto a los derechos de autor sobre la obra en cuestión, EL AUTOR, asumirá toda la responsabilidad, y saldrá en defensa de los derechos aquí autorizados; para todos los efectos la ESCUELA DE INGENIERÍA DE ANTIOQUIA actúa como un tercero de buena fe.info:eu-repo/semantics/openAccessAtribución-NoComercialhttp://purl.org/coar/access_right/c_abf2RBI00073TECNOLOGÍAS PARA LA SALUDTECHNOLOGY IN HEALTHANTIBODIES - PHYSIOLOGYANTICUERPOS - FISIOLOGÍAANTIBODY ENGINEERINGPHASE DISPLAYCELL DISPLAYRIBOSOME DISPLAYANTIBODY HUMANIZATIONINGENIERIA DE ANTICUERPOSPRESENTACION EN FAGOSPRESENTACION EN CELULASPRESENTACION EN RIBOSOMASHUMANIZACION DE ANTICUERPOSEngineering antibody fragments: replicating the immune system and beyondLa ingeniería de fragmentos de anticuerpos: imitando y expandiendo el sistema inmuneArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionTexthttps://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85Biomédica, Mecatrónica y MecánicaEscuela de Ingeniería de Antioquia EIAUniversidad CESCarter P.J. Potent antibody therapeutics by design. Nature reviews. Immunology, 6:343-357, 2006.Filpula D. Antibody engineering and modifi cation technologies. Biomolecular engineering, 24:201-215, 2007.Salfeld J.G. Isotype selection in antibody engineering. Nature biotechnology, 25:1369-1372, 2007.PublicationTHUMBNAILRBI00073.pdf.jpgRBI00073.pdf.jpgGenerated Thumbnailimage/jpeg13594https://repository.eia.edu.co/bitstreams/f1d70eef-834a-4ad5-96af-5b57ee3db36a/download49d4f444102bd662715bd08644a17078MD57ORIGINALRBI00073.pdfRBI00073.pdfapplication/pdf1606887https://repository.eia.edu.co/bitstreams/a8c3a87c-7061-4b0e-a558-7dbe9797f1ee/download0f3efb7b79d3ae4fa4f4128a15aa473dMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81494https://repository.eia.edu.co/bitstreams/fcd4d9da-d7e9-4577-a3bb-8891d97ee0aa/download66874b0b9366b748c60895d2fb6339f8MD52CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849https://repository.eia.edu.co/bitstreams/6ae28411-0e5d-4df5-8e9b-27c170ac6450/download4afdbb8c545fd630ea7db775da747b2fMD53license_textlicense_texttext/html; charset=utf-821251https://repository.eia.edu.co/bitstreams/197647a5-4ac1-4040-bd88-a8bbc9f05883/download251affa47a4b8eb28fc5a9a9249263a1MD54license_rdflicense_rdfapplication/rdf+xml; charset=utf-823253https://repository.eia.edu.co/bitstreams/23999844-2424-4f05-b0aa-99cdeee7c972/downloadcd76e7886171c964e259dcf5e912e299MD55TEXTRBI00073.pdf.txtRBI00073.pdf.txtExtracted texttext/plain60547https://repository.eia.edu.co/bitstreams/6d029aa0-d496-409c-a1f3-8516d5f5925b/download2abcc879e37c7f1cab78e5a828ea961dMD5611190/484oai:repository.eia.edu.co:11190/4842023-07-25 16:47:59.793https://creativecommons.org/licenses/by-nc/4.0/Derechos Reservados - Universidad EIA, 2020open.accesshttps://repository.eia.edu.coRepositorio Institucional Universidad EIAbdigital@metabiblioteca.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 |