Oral administration of carvacrol/β-cyclodextrin complex protects against 6-hydroxydopamine-induced dopaminergic denervation

Carvacrol (CARV) presents valuable biological properties such as anti-inflammatory and antioxidant activities. However, pharmacological uses of CARV are largely limited due to disadvantages related to solubility, bioavailability, preparation and storage processes. The complexation of monoterpenes with β-cyclodextrin (β-CD) increases their stability, solubility and oral bioavailability. Here, the protective effect of oral treatment with CARV/β-CD complex (25 μg/kg/day) against dopaminergic (DA) denervation induced by unilateral intranigral injection of 6-hydroxydopamine (6-OHDA - 10 μg per rat) was analyzed, in order to evaluate a putative application in the development of neuroprotective therapies for Parkinson's disease (PD). Pretreatment with CARV/β-CD for 15 days prevented the loss of DA neurons induced by 6-OHDA in adult Wistar rats. This effect may occur through CARV anti-inflammatory and antioxidant properties, as the pretreatment with CARV/β-CD inhibited the release of IL-1β and TNF-α; besides, CARV prevented the increase of mitochondrial superoxide production induced by 6-OHDA in cultured SH-SY5Y cells. Importantly, hepatotoxicity or alterations in blood cell profile were not observed with oral administration of CARV/β-CD. Therefore, this study showed a potential pharmacological application of CARV/β-CD in PD using a non-invasive route of drug delivery, i.e., oral administration.

• Autores: Tiefensee Ribeiro, Camila; Gasparotto, Juciano; Lintzmaier Petiz, Lyvia; Ozorio Brum, Pedro; Oppermann Peixoto, Daniel; Kunzler, Alice; Tais da Rosa Silva, Helen; Calixto Bortolin, Rafael; Farina Almeida, Roberto; Quintans-Junior, Lucindo José; Antunes Araújo, Adriano; Fonseca Moreira, José Claudio; Pens Gelain, Daniel
• Tipo de recurso: http://purl.org/coar/resource_type/c_816b
• Fecha de publicación: 2019
• Institución: Corporación Universidad de la Costa
• Repositorio: REDICUC - Repositorio CUC
• Idioma: eng
• OAI Identifier: oai:repositorio.cuc.edu.co:11323/2993
• Acceso en línea: https://hdl.handle.net/11323/2993; https://repositorio.cuc.edu.co/
• Palabra clave: Carvacrol; β-cyclodextrin; 6-hydroxydopamine; Parkinson’s disease
• Rights: openAccess
• License: Atribución – No comercial – Compartir igual