A bump-hole strategy for increased stringency of cell-specific metabolic labeling of rna
Profiling RNA expression in a cell-specific manner continues to be a grand challenge in biochemical research. Bioorthogonal nucleosides can be utilized to track RNA expression; however, these methods currently have limitations due to background and incorporation of analogs into undesired cells. Here...
- Autores:
-
Nguyen, Kim
Kubota, Miles
Del Arco, Jon
Feng, Chao
Singha, Monika
Beasley, Samantha
Sakr, Jasmine
P. Gandhi, Sunil
Blurton-Jones, Mathew
Fernández Lucas, Jesus
C. Spitale, Robert
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2020
- Institución:
- Corporación Universidad de la Costa
- Repositorio:
- REDICUC - Repositorio CUC
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.cuc.edu.co:11323/7879
- Acceso en línea:
- https://hdl.handle.net/11323/7879
https://doi.org/10.1021/acschembio.0c00755
https://repositorio.cuc.edu.co/
- Palabra clave:
- Peptides and proteins
Genetics
Labeling
Uracil
Imaging probes
- Rights
- embargoedAccess
- License
- Attribution-NonCommercial-NoDerivatives 4.0 International
| Summary: | Profiling RNA expression in a cell-specific manner continues to be a grand challenge in biochemical research. Bioorthogonal nucleosides can be utilized to track RNA expression; however, these methods currently have limitations due to background and incorporation of analogs into undesired cells. Herein, we design and demonstrate that uracil phosphoribosyltransferase can be engineered to match 5-vinyluracil for cell-specific metabolic labeling of RNA with exceptional specificity and stringency. |
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