Cytotoxicity and genotoxicity induced by coal and coal fly ash particles samples in V79 cells
Exposure to coal and coal ashes can cause harmful effects in in vitro and in vivo systems, mainly by the induction of oxidative damage. The aim of this work was to assess cytotoxic and genotoxic effects using the V79 cell line treated with coal and coal fly ash particles derived from a coal power pl...
- Autores:
-
León Mejía, Grethel
Silva Oliveira, Luis Felipe
Civeira, Matheus S.
Silva Oliveira, Marcos Leandro
Da Silva Machado, Miriana
Villela, Izabel Vianna
Hartmann, Andreas
Premoli, Suziane M.
Da Silva, Juliana
Pegas Henriques, Joao Antonio
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2016
- Institución:
- Corporación Universidad de la Costa
- Repositorio:
- REDICUC - Repositorio CUC
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.cuc.edu.co:11323/4207
- Acceso en línea:
- https://hdl.handle.net/11323/4207
https://repositorio.cuc.edu.co/
- Palabra clave:
- Coal
Coal fly ashes
DNA oxidative damage
Comet assay
Micronucleus assay
Carbón
Cenizas de la mosca de carbón
Daño oxidativo del ADN
Ensayo cometa
Ensayo de micronúcleos
- Rights
- openAccess
- License
- Attribution-NonCommercial-ShareAlike 4.0 International
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| dc.title.spa.fl_str_mv |
Cytotoxicity and genotoxicity induced by coal and coal fly ash particles samples in V79 cells |
| dc.title.translated.spa.fl_str_mv |
Citotoxicidad y genotoxicidad inducidas por muestras de partículas de carbón y cenizas volantes en células V79 |
| title |
Cytotoxicity and genotoxicity induced by coal and coal fly ash particles samples in V79 cells |
| spellingShingle |
Cytotoxicity and genotoxicity induced by coal and coal fly ash particles samples in V79 cells Coal Coal fly ashes DNA oxidative damage Comet assay Micronucleus assay Carbón Cenizas de la mosca de carbón Daño oxidativo del ADN Ensayo cometa Ensayo de micronúcleos |
| title_short |
Cytotoxicity and genotoxicity induced by coal and coal fly ash particles samples in V79 cells |
| title_full |
Cytotoxicity and genotoxicity induced by coal and coal fly ash particles samples in V79 cells |
| title_fullStr |
Cytotoxicity and genotoxicity induced by coal and coal fly ash particles samples in V79 cells |
| title_full_unstemmed |
Cytotoxicity and genotoxicity induced by coal and coal fly ash particles samples in V79 cells |
| title_sort |
Cytotoxicity and genotoxicity induced by coal and coal fly ash particles samples in V79 cells |
| dc.creator.fl_str_mv |
León Mejía, Grethel Silva Oliveira, Luis Felipe Civeira, Matheus S. Silva Oliveira, Marcos Leandro Da Silva Machado, Miriana Villela, Izabel Vianna Hartmann, Andreas Premoli, Suziane M. Da Silva, Juliana Pegas Henriques, Joao Antonio |
| dc.contributor.author.spa.fl_str_mv |
León Mejía, Grethel Silva Oliveira, Luis Felipe Civeira, Matheus S. Silva Oliveira, Marcos Leandro Da Silva Machado, Miriana Villela, Izabel Vianna Hartmann, Andreas Premoli, Suziane M. Da Silva, Juliana Pegas Henriques, Joao Antonio |
| dc.subject.spa.fl_str_mv |
Coal Coal fly ashes DNA oxidative damage Comet assay Micronucleus assay Carbón Cenizas de la mosca de carbón Daño oxidativo del ADN Ensayo cometa Ensayo de micronúcleos |
| topic |
Coal Coal fly ashes DNA oxidative damage Comet assay Micronucleus assay Carbón Cenizas de la mosca de carbón Daño oxidativo del ADN Ensayo cometa Ensayo de micronúcleos |
| description |
Exposure to coal and coal ashes can cause harmful effects in in vitro and in vivo systems, mainly by the induction of oxidative damage. The aim of this work was to assess cytotoxic and genotoxic effects using the V79 cell line treated with coal and coal fly ash particles derived from a coal power plant located in Santa Catarina, Brazil. Two coal samples (COAL11 and COAL16) and two coal fly ash samples (CFA11 and CFA16) were included in this study. COAL16 was co-firing with a mixture of fuel oil and diesel oil. The comet assay data showed that exposure of V79 cells to coal and coal fly ash particles induced primary DNA lesions. Application of lesion-specific endonucleases (FPG and ENDO III) demonstrated increased DNA effects indicating the presence of high amounts of oxidative DNA lesions. The cytokinesis-block micronucleus cytome assay analysis showed that exposure of V79 cells to high concentrations of coal and coal fly ash particles induced cytotoxic effects (apoptosis and necrosis) and chromosomal instability (nucleoplasmic bridges, nuclear buds, and micronucleus (MN) formation). These results may be associated with compounds contained in the surface of the particles as hazardous elements, ultrafine/nanoparticles, and polycyclic aromatic hydrocarbons (PAHs) which were detected in the samples. |
| publishDate |
2016 |
| dc.date.issued.none.fl_str_mv |
2016-09-07 |
| dc.date.accessioned.none.fl_str_mv |
2019-05-17T15:08:48Z |
| dc.date.available.none.fl_str_mv |
2019-05-17T15:08:48Z |
| dc.type.spa.fl_str_mv |
Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/resource_type/c_6501 |
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Text |
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info:eu-repo/semantics/article |
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http://purl.org/redcol/resource_type/ART |
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acceptedVersion |
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https://hdl.handle.net/11323/4207 |
| dc.identifier.instname.spa.fl_str_mv |
Corporación Universidad de la Costa |
| dc.identifier.reponame.spa.fl_str_mv |
REDICUC - Repositorio CUC |
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https://repositorio.cuc.edu.co/ |
| url |
https://hdl.handle.net/11323/4207 https://repositorio.cuc.edu.co/ |
| identifier_str_mv |
Corporación Universidad de la Costa REDICUC - Repositorio CUC |
| dc.language.iso.none.fl_str_mv |
eng |
| language |
eng |
| dc.rights.spa.fl_str_mv |
Attribution-NonCommercial-ShareAlike 4.0 International |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidad de la Costa |
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Corporación Universidad de la Costa |
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León Mejía, GrethelSilva Oliveira, Luis FelipeCiveira, Matheus S.Silva Oliveira, Marcos LeandroDa Silva Machado, MirianaVillela, Izabel ViannaHartmann, AndreasPremoli, Suziane M.Da Silva, JulianaPegas Henriques, Joao Antonio2019-05-17T15:08:48Z2019-05-17T15:08:48Z2016-09-07https://hdl.handle.net/11323/4207Corporación Universidad de la CostaREDICUC - Repositorio CUChttps://repositorio.cuc.edu.co/Exposure to coal and coal ashes can cause harmful effects in in vitro and in vivo systems, mainly by the induction of oxidative damage. The aim of this work was to assess cytotoxic and genotoxic effects using the V79 cell line treated with coal and coal fly ash particles derived from a coal power plant located in Santa Catarina, Brazil. Two coal samples (COAL11 and COAL16) and two coal fly ash samples (CFA11 and CFA16) were included in this study. COAL16 was co-firing with a mixture of fuel oil and diesel oil. The comet assay data showed that exposure of V79 cells to coal and coal fly ash particles induced primary DNA lesions. Application of lesion-specific endonucleases (FPG and ENDO III) demonstrated increased DNA effects indicating the presence of high amounts of oxidative DNA lesions. The cytokinesis-block micronucleus cytome assay analysis showed that exposure of V79 cells to high concentrations of coal and coal fly ash particles induced cytotoxic effects (apoptosis and necrosis) and chromosomal instability (nucleoplasmic bridges, nuclear buds, and micronucleus (MN) formation). These results may be associated with compounds contained in the surface of the particles as hazardous elements, ultrafine/nanoparticles, and polycyclic aromatic hydrocarbons (PAHs) which were detected in the samples.La exposición al carbón y las cenizas de carbón puede causar efectos nocivos en sistemas in vitro e in vivo, principalmente por la inducción de daño oxidativo. El objetivo de este trabajo fue evaluar los efectos citotóxicos y genotóxicos utilizando la línea celular V79 tratada con partículas de carbón y cenizas volantes derivadas de una central eléctrica de carbón ubicada en Santa Catarina, Brasil. En este estudio se incluyeron dos muestras de carbón (COAL11 y COAL16) y dos muestras de cenizas volantes (CFA11 y CFA16). COAL16 fue de combustión conjunta con una mezcla de fuel oil y diesel oil. Los datos del ensayo del cometa mostraron que la exposición de células V79 a partículas de carbón y cenizas volantes de carbón indujo lesiones primarias de ADN. La aplicación de endonucleasas específicas de la lesión (FPG y ENDO III) demostró un aumento en los efectos del ADN que indica la presencia de altas cantidades de lesiones oxidativas del ADN. El análisis del ensayo del citoma micronúcleo del bloque de citocinesis mostró que la exposición de células V79 a altas concentraciones de partículas de carbón y cenizas volantes de carbón indujo efectos citotóxicos (apoptosis y necrosis) e inestabilidad cromosómica (puentes nucleoplásmicos, brotes nucleares y formación de micronúcleo (MN)). Estos resultados pueden asociarse con compuestos contenidos en la superficie de las partículas como elementos peligrosos, ultrafinos / nanopartículas e hidrocarburos aromáticos policíclicos (HAP) que se detectaron en las muestras.León Mejía, Grethel-04e07733-ff27-4faa-940a-e0fbb78ed491-0Silva Oliveira, Luis Felipe-5c1c9b54-f160-4f3c-bcd5-b9f532c778af-0Civeira, Matheus S.-ce190c10-bb0c-48e9-8265-c1e24463e5db-0Silva Oliveira, Marcos Leandro-0000-0001-6044-8737-600Da Silva Machado, Miriana-d3c5ec55-55f5-4736-9acc-f7d7f70207c7-0Villela, Izabel Vianna-5a301479-caa8-4f31-a7cc-20d302e2a8ff-0Hartmann, Andreas-2a9c64dc-1c2f-42f9-9a48-792edce2805e-0Premoli, Suziane M.-04d29a64-49ba-4d3d-8526-10aec29bd97c-0Da Silva, Juliana-f7debe6e-a31f-4d44-bad3-217b756a267e-0Pegas Henriques, Joao Antonio-f7bff852-0e82-4b56-953a-f06ad2cd2a6c-0engUniversidad de la CostaAttribution-NonCommercial-ShareAlike 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2CoalCoal fly ashesDNA oxidative damageComet assayMicronucleus assayCarbónCenizas de la mosca de carbónDaño oxidativo del ADNEnsayo cometaEnsayo de micronúcleosCytotoxicity and genotoxicity induced by coal and coal fly ash particles samples in V79 cellsCitotoxicidad y genotoxicidad inducidas por muestras de partículas de carbón y cenizas volantes en células V79Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/acceptedVersionPublicationORIGINALCytotoxicity and genotoxicity induced by coal and coal fly ash particles samples in V79 cells.pdfCytotoxicity and genotoxicity induced by coal and coal fly ash particles samples in V79 cells.pdfapplication/pdf203325https://repositorio.cuc.edu.co/bitstreams/2851c313-f6ed-40df-b404-b540fb7404a3/downloadd619e060ee856e7de846e4285b4c0aa3MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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