Effect of stressful conditions on the carotenogenic activity of a Colombian strain of Dunaliella salina
The objective was evaluate the carotenogenic activity ofDunaliella salinaisolated from the artificial saltflats of municipality of Manaure (Department of La Guajira, Colombia). Two experimental testings weredesigned, in triplicate, to induce the reversibility of the cell tonality depending on the cu...
- Autores:
-
Gallego Cartagena, Euler
Castillo-Ramírez, Margarita
Martínez-Burgos, Walter
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2019
- Institución:
- Corporación Universidad de la Costa
- Repositorio:
- REDICUC - Repositorio CUC
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.cuc.edu.co:11323/5138
- Acceso en línea:
- https://hdl.handle.net/11323/5138
https://repositorio.cuc.edu.co/
- Palabra clave:
- Dunaliella salina
Carotenoides
Stressful conditions
Biotechnological purposes
- Rights
- openAccess
- License
- CC0 1.0 Universal
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| dc.title.spa.fl_str_mv |
Effect of stressful conditions on the carotenogenic activity of a Colombian strain of Dunaliella salina |
| title |
Effect of stressful conditions on the carotenogenic activity of a Colombian strain of Dunaliella salina |
| spellingShingle |
Effect of stressful conditions on the carotenogenic activity of a Colombian strain of Dunaliella salina Dunaliella salina Carotenoides Stressful conditions Biotechnological purposes |
| title_short |
Effect of stressful conditions on the carotenogenic activity of a Colombian strain of Dunaliella salina |
| title_full |
Effect of stressful conditions on the carotenogenic activity of a Colombian strain of Dunaliella salina |
| title_fullStr |
Effect of stressful conditions on the carotenogenic activity of a Colombian strain of Dunaliella salina |
| title_full_unstemmed |
Effect of stressful conditions on the carotenogenic activity of a Colombian strain of Dunaliella salina |
| title_sort |
Effect of stressful conditions on the carotenogenic activity of a Colombian strain of Dunaliella salina |
| dc.creator.fl_str_mv |
Gallego Cartagena, Euler Castillo-Ramírez, Margarita Martínez-Burgos, Walter |
| dc.contributor.author.spa.fl_str_mv |
Gallego Cartagena, Euler Castillo-Ramírez, Margarita Martínez-Burgos, Walter |
| dc.subject.spa.fl_str_mv |
Dunaliella salina Carotenoides Stressful conditions Biotechnological purposes |
| topic |
Dunaliella salina Carotenoides Stressful conditions Biotechnological purposes |
| description |
The objective was evaluate the carotenogenic activity ofDunaliella salinaisolated from the artificial saltflats of municipality of Manaure (Department of La Guajira, Colombia). Two experimental testings weredesigned, in triplicate, to induce the reversibility of the cell tonality depending on the culture conditions.In the first test (A), to induce the reversibility from green to red tonality inD. salinacells, these were cul-tured in J/1 medium at a concentration of 4.0 M NaCl, 390mmol m 2s 1, 0.50 mM KNO3. In the second test(B), to induce the reversibility from red to green cell tonality, the cultures were maintained in J/1 medium1 M NaCl, 190mmol m 2s 1, 5.0 mM KNO3and pH 8.2. The population growth was evaluated by cell countand the pigment content was performed by spectrophotometric techniques. It was found that in both teststhe culture conditions influenced the population growth and the pigments production ofD. salina. Therewas a significant difference between the mean values of total carotenoids in the test A with 9.67 ± 0.19lg/ml and second test with 1.54 ± 0.08lg/ml at a significance level of p < 0.05. It was demonstrated thatthe culture conditions of test A induce the production of lipophilic antioxidants, among these carotenoids.The knowledge of the stressful conditions for the production of carotenoids fromD. salinaisolated fromartificial saline of Manaure opens a field in implementation of this biotic resource for biotechnological pur-poses, production of new antibiotics, nutraceuticals and/or biofuels production. |
| publishDate |
2019 |
| dc.date.accessioned.none.fl_str_mv |
2019-08-09T15:23:21Z |
| dc.date.available.none.fl_str_mv |
2019-08-09T15:23:21Z |
| dc.date.issued.none.fl_str_mv |
2019-07-23 |
| dc.type.spa.fl_str_mv |
Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/resource_type/c_6501 |
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Text |
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info:eu-repo/semantics/article |
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http://purl.org/redcol/resource_type/ART |
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info:eu-repo/semantics/acceptedVersion |
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1319-562X |
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https://hdl.handle.net/11323/5138 |
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Corporación Universidad de la Costa |
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REDICUC - Repositorio CUC |
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https://repositorio.cuc.edu.co/ |
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1319-562X Corporación Universidad de la Costa REDICUC - Repositorio CUC |
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https://hdl.handle.net/11323/5138 https://repositorio.cuc.edu.co/ |
| dc.language.iso.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartof.spa.fl_str_mv |
https://doi.org/10.1016/j.sjbs.2019.07.010 |
| dc.relation.references.spa.fl_str_mv |
Andersen and Kawachi, 2005 R.A. Andersen, M. Kawachi Traditional microalgae isolation techniques Algal Cult. Tech., 83 (2005), pp. 90-101 CrossRefView Record in ScopusGoogle Scholar Azachi et al., 2002 M. Azachi, A. Sadka, M. Fisher, P. Goldshlag, I. Gokhman, A. Zamir Salt induction of fatty acid elongase and membrane lipid modifications in the extreme halotolerant alga Dunaliella salina Plant Physiol., 129 (2002), pp. 1320-1329 CrossRefView Record in ScopusGoogle Scholar Ben-Amotz and Avron, 1983 A. Ben-Amotz, M. Avron On the factors which determine massive β-carotene accumulation in the halotolerant alga Dunaliella bardawil Plant Physiol., 7 (1983), pp. 593-597 CrossRefView Record in ScopusGoogle Scholar Benemann, 2013 J. Benemann Microalgae for biofuels and animal feeds Energies, 6 (2013), pp. 5869-5886 CrossRefView Record in ScopusGoogle Scholar Bhattacharjee, 2016 M. Bhattacharjee Pharmaceutically valuable bioactive compounds of algae Asian J. Pharm. Clin. Res., 7 (2016), pp. 43-47 CrossRefView Record in ScopusGoogle Scholar Borowitzka, 1988 M. Borowitzka Algal growth media and sources of algal cultures M.A. Borowitzka, L.J. Borowitzka (Eds.), Microalgal Biotechnology, Cambridge University Press, Cambridge (1988), pp. 456-465 Google Scholar Borowitzka and Borowitzka, 1988 M. Borowitzka, L. Borowitzka Limits to growth and carotenogenesis in laboratory and large-scale outdoor cultures of Dunaliella salina T. Stadler, J. Mollion, M. Verdus, Y. Karamanos, H. Morvan, D. Christiaen (Eds.), Algal Biotechnology, Elsevier Applied Science, Barking, UK (1988), pp. 371-381 View Record in ScopusGoogle Scholar Borowitzka, 1995 M. Borowitzka Microalgae as sources of pharmaceuticals and other biologically active compounds J. Appl. Phycol., 7 (1995), pp. 3-15 CrossRefView Record in ScopusGoogle Scholar Chen and Jiang, 2009 H. Chen, J. Jiang Osmotic responses of Dunaliella to the changes of salinity J. Cell Physiol., 219 (2009), pp. 251-258 CrossRefView Record in ScopusGoogle Scholar Chisti, 2007 Y. Chisti Biodiesel from microalgae Biotechnol. Adv., 25 (2007), pp. 294-306 ArticleDownload PDFView Record in ScopusGoogle Scholar Cifuentes et al., 1996 A. Cifuentes, M. Gonzalez, O. Parra, M. Zúñiga Cultivo de cepas de Dunaliella salina (Teodoresco 1905) en diferentes medios bajo condiciones de laboratorio Rev. Chil. Hist. Nat., 69 (1996), pp. 105-112 View Record in ScopusGoogle Scholar Cowan and Rose, 1991 A. Cowan, P. Rose Abscisic acid metabolism in salt-stressed cells of Dunaliella salina: possible interrelationship with β-carotene accumulation Plant Physiol., 97 (1991), pp. 798-803 CrossRefView Record in ScopusGoogle Scholar Cowan and Rose, 1992 A. Cowan, P. Rose Horne L. Dunaliella salina: a model system for studying the response of plant cells to stress J. Exp. Bot., 43 (1992), pp. 1535-1547 CrossRefView Record in ScopusGoogle Scholar Deng and Coleman, 1999 M. Deng, J. Coleman Ethanol synthesis by genetic engineering in cyanobacteria Appl. Environ. Microb., 65 (1999), pp. 523-528 View Record in ScopusGoogle Scholar Dhanam and Dhandayuthapani, 2013 D. Dhanam, K. Dhandayuthapani Optimization of β-carotene production by marine microalga Dunaliella salina Int. J. Curr. Microbiol. App. Sci., 3 (2013), pp. 37-43 View Record in ScopusGoogle Scholar Dipak and Lele, 2005 P. Dipak, S. Lele Carotenoid production from microalga Dunaliella salina Indian J. Biotechnol., 4 (2005), pp. 476-483 Google Scholar El-Baky et al., 2004 A. El-Baky, F. El-Baz, G. El-Baroty Production of antioxidant by the green alga Dunaliella salina Int. J. Agric. Biol., 6 (2004), pp. 49-57 Google Scholar Emtyazjoo et al., 2012 M. Emtyazjoo, Z. Moghadasi, M. Rabbani, M. Emtyazjoo, S. Samadi, N. Mossaffa Anticancer effect of Dunaliella salina under stress and normal conditions against skin carcinoma cell line A431 in vitro Iran. J. Fish Sci., 11 (2012), pp. 283-293 Google Scholar Eriksen, 2008 N. Eriksen The technology of microalgal culturing Biotechnol. Lett., 30 (2008), pp. 1525-1536 CrossRefView Record in ScopusGoogle Scholar Ettl, 1983 H. Ettl Taxonomische bemerkungen zu den Phytomonadina Nova Hedwigia, 35 (1983), pp. 731-736 View Record in ScopusGoogle Scholar Faheed and Fattah, 2008 F. Faheed, Z. Fattah Effect of Chlorella vulgaris as biofertilizer on growth parameters and metabolic aspects of Lettus plant J. Agr. Soc. Sci., 4 (2008), pp. 165-169 View Record in ScopusGoogle Scholar Fazeli et al., 2006 M. Fazeli, H. Tofighi, N. Samadi, H. Jamalifar, A. Fazeli Carotenoids accumulation by Dunaliella tertiolecta (Lake Urmia isolate) and Dunaliella salina (CCAP 19/18 & WT) under stress conditions DARU, 14 (2006), pp. 146-150 View Record in ScopusGoogle Scholar Fu et al., 2014 W. Fu, G. Paglia, M. Magnúsdóttir, E. Steinarsdóttir, S. Gudmundsson, B. Palsson, S. Brynjólfsson, O. Andrésson, S. Brynjólfsson Effects of abiotic stressors on lutein production in the green microalga Dunaliella salina Microb. Cell Fact., 13 (2014), pp. 1-9 View Record in ScopusGoogle Scholar Gallego et al., 2013 E. Gallego, L. Manjarrez, L. Herrera Effect of subbituminous coal on growth and pigments concentration of Dunaliella salina (Teodoresco, 1905) cultivated in photobioreactor multiple chambers oscillating Intropica, 8 (2013), pp. 69-78 View Record in ScopusGoogle Scholar Gimpel et al., 2013 J. Gimpel, E. Specht, D. Georgianna, S. Mayfield Advances in microalgae engineering and synthetic biology applications for biofuel production Curr. Opin. Chem. Biol., 17 (2013), pp. 489-495 ArticleDownload PDFView Record in ScopusGoogle Scholar Ginzburg, 1987 M. Ginzburg Dunaliella: a green alga adapted to salt Adv. Bot. Res., 14 (1987), pp. 93-183 Google Scholar Giordano and Bowes, 1997 M. Giordano, G. 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Hallmann Algal transgenics and biotechnology Transgenic Plant J., 1 (2007), pp. 81-98 View Record in ScopusGoogle Scholar Hemaiswarya et al., 2011 S. Hemaiswarya, R. Raja, R. Kumar, V. Ganesan, C. Anbazhagan Microalgae: a sustainable feed source for aquaculture World J. Microb. Biot., 27 (2011), pp. 1737-1746 CrossRefView Record in ScopusGoogle Scholar Jeffrey and Humphrey, 1975 S. Jeffrey, G. Humphrey New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton Biochem. Physiol. Pflanzen., 167 (1975), pp. 191-194 ArticleDownload PDFView Record in ScopusGoogle Scholar Khoyi et al., 2009 Z. Khoyi, J. Seyfabadi, Z. Ramezanpour Effects of light intensity and photoperiod on the growth rate, chlorophyll a and β-carotene of freshwater green microalga Chlorella vulgaris. Comparative biochemistry and physiology Part A Mol. Integr. Physiol., 2 (2009), pp. 210-215 Google Scholar Kleinegris et al., 2011 D. Kleinegris, M. 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Gallego Cartagena, EulerCastillo-Ramírez, MargaritaMartínez-Burgos, Walter2019-08-09T15:23:21Z2019-08-09T15:23:21Z2019-07-231319-562Xhttps://hdl.handle.net/11323/5138Corporación Universidad de la CostaREDICUC - Repositorio CUChttps://repositorio.cuc.edu.co/The objective was evaluate the carotenogenic activity ofDunaliella salinaisolated from the artificial saltflats of municipality of Manaure (Department of La Guajira, Colombia). Two experimental testings weredesigned, in triplicate, to induce the reversibility of the cell tonality depending on the culture conditions.In the first test (A), to induce the reversibility from green to red tonality inD. salinacells, these were cul-tured in J/1 medium at a concentration of 4.0 M NaCl, 390mmol m 2s 1, 0.50 mM KNO3. In the second test(B), to induce the reversibility from red to green cell tonality, the cultures were maintained in J/1 medium1 M NaCl, 190mmol m 2s 1, 5.0 mM KNO3and pH 8.2. The population growth was evaluated by cell countand the pigment content was performed by spectrophotometric techniques. It was found that in both teststhe culture conditions influenced the population growth and the pigments production ofD. salina. Therewas a significant difference between the mean values of total carotenoids in the test A with 9.67 ± 0.19lg/ml and second test with 1.54 ± 0.08lg/ml at a significance level of p < 0.05. It was demonstrated thatthe culture conditions of test A induce the production of lipophilic antioxidants, among these carotenoids.The knowledge of the stressful conditions for the production of carotenoids fromD. salinaisolated fromartificial saline of Manaure opens a field in implementation of this biotic resource for biotechnological pur-poses, production of new antibiotics, nutraceuticals and/or biofuels production.Gallego Cartagena, EulerCastillo-Ramírez, MargaritaMartínez-Burgos, WalterengSaudi Journal of Biological Scienceshttps://doi.org/10.1016/j.sjbs.2019.07.010Andersen and Kawachi, 2005 R.A. Andersen, M. Kawachi Traditional microalgae isolation techniques Algal Cult. Tech., 83 (2005), pp. 90-101 CrossRefView Record in ScopusGoogle Scholar Azachi et al., 2002 M. Azachi, A. Sadka, M. Fisher, P. Goldshlag, I. Gokhman, A. Zamir Salt induction of fatty acid elongase and membrane lipid modifications in the extreme halotolerant alga Dunaliella salina Plant Physiol., 129 (2002), pp. 1320-1329 CrossRefView Record in ScopusGoogle Scholar Ben-Amotz and Avron, 1983 A. Ben-Amotz, M. Avron On the factors which determine massive β-carotene accumulation in the halotolerant alga Dunaliella bardawil Plant Physiol., 7 (1983), pp. 593-597 CrossRefView Record in ScopusGoogle Scholar Benemann, 2013 J. Benemann Microalgae for biofuels and animal feeds Energies, 6 (2013), pp. 5869-5886 CrossRefView Record in ScopusGoogle Scholar Bhattacharjee, 2016 M. Bhattacharjee Pharmaceutically valuable bioactive compounds of algae Asian J. Pharm. Clin. Res., 7 (2016), pp. 43-47 CrossRefView Record in ScopusGoogle Scholar Borowitzka, 1988 M. Borowitzka Algal growth media and sources of algal cultures M.A. Borowitzka, L.J. Borowitzka (Eds.), Microalgal Biotechnology, Cambridge University Press, Cambridge (1988), pp. 456-465 Google Scholar Borowitzka and Borowitzka, 1988 M. Borowitzka, L. Borowitzka Limits to growth and carotenogenesis in laboratory and large-scale outdoor cultures of Dunaliella salina T. Stadler, J. Mollion, M. Verdus, Y. Karamanos, H. Morvan, D. Christiaen (Eds.), Algal Biotechnology, Elsevier Applied Science, Barking, UK (1988), pp. 371-381 View Record in ScopusGoogle Scholar Borowitzka, 1995 M. Borowitzka Microalgae as sources of pharmaceuticals and other biologically active compounds J. Appl. Phycol., 7 (1995), pp. 3-15 CrossRefView Record in ScopusGoogle Scholar Chen and Jiang, 2009 H. Chen, J. Jiang Osmotic responses of Dunaliella to the changes of salinity J. Cell Physiol., 219 (2009), pp. 251-258 CrossRefView Record in ScopusGoogle Scholar Chisti, 2007 Y. Chisti Biodiesel from microalgae Biotechnol. Adv., 25 (2007), pp. 294-306 ArticleDownload PDFView Record in ScopusGoogle Scholar Cifuentes et al., 1996 A. Cifuentes, M. Gonzalez, O. Parra, M. Zúñiga Cultivo de cepas de Dunaliella salina (Teodoresco 1905) en diferentes medios bajo condiciones de laboratorio Rev. Chil. Hist. Nat., 69 (1996), pp. 105-112 View Record in ScopusGoogle Scholar Cowan and Rose, 1991 A. Cowan, P. Rose Abscisic acid metabolism in salt-stressed cells of Dunaliella salina: possible interrelationship with β-carotene accumulation Plant Physiol., 97 (1991), pp. 798-803 CrossRefView Record in ScopusGoogle Scholar Cowan and Rose, 1992 A. Cowan, P. Rose Horne L. Dunaliella salina: a model system for studying the response of plant cells to stress J. Exp. Bot., 43 (1992), pp. 1535-1547 CrossRefView Record in ScopusGoogle Scholar Deng and Coleman, 1999 M. Deng, J. Coleman Ethanol synthesis by genetic engineering in cyanobacteria Appl. Environ. 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