Giardia duodenalis genotyping not linked to clinical symptomatology and nutritional status of school-aged children of Soledad and Galapa municipality schools, Atlántico, Colombia
Giardia duodenalis genotypes A and B have been reported in Colombia. The population consisted of 235 schoolchildren whose ages ranged from 2 to 10 yr of age from the municipalities of Soledad and Galapa in the department of Atlántico, Colombia. Fecal samples were obtained and then analyzed in tripli...
- Autores:
-
Muñoz Salas, Karen Esther
Polo Barrios, Alma
Maestre Gonzalez, Carolina
Rodríguez Macias, Juan
Vargas-Zapata, Carmiña Lucía
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2022
- Institución:
- Corporación Universidad de la Costa
- Repositorio:
- REDICUC - Repositorio CUC
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.cuc.edu.co:11323/9156
- Acceso en línea:
- https://hdl.handle.net/11323/9156
https://doi.org/10.1645/21-26
https://repositorio.cuc.edu.co/
- Palabra clave:
- Giardia duodenalis
Genotypes
Children
Giardiasis
Malnutrition
Clinical manifestations
- Rights
- embargoedAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
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| dc.title.eng.fl_str_mv |
Giardia duodenalis genotyping not linked to clinical symptomatology and nutritional status of school-aged children of Soledad and Galapa municipality schools, Atlántico, Colombia |
| title |
Giardia duodenalis genotyping not linked to clinical symptomatology and nutritional status of school-aged children of Soledad and Galapa municipality schools, Atlántico, Colombia |
| spellingShingle |
Giardia duodenalis genotyping not linked to clinical symptomatology and nutritional status of school-aged children of Soledad and Galapa municipality schools, Atlántico, Colombia Giardia duodenalis Genotypes Children Giardiasis Malnutrition Clinical manifestations |
| title_short |
Giardia duodenalis genotyping not linked to clinical symptomatology and nutritional status of school-aged children of Soledad and Galapa municipality schools, Atlántico, Colombia |
| title_full |
Giardia duodenalis genotyping not linked to clinical symptomatology and nutritional status of school-aged children of Soledad and Galapa municipality schools, Atlántico, Colombia |
| title_fullStr |
Giardia duodenalis genotyping not linked to clinical symptomatology and nutritional status of school-aged children of Soledad and Galapa municipality schools, Atlántico, Colombia |
| title_full_unstemmed |
Giardia duodenalis genotyping not linked to clinical symptomatology and nutritional status of school-aged children of Soledad and Galapa municipality schools, Atlántico, Colombia |
| title_sort |
Giardia duodenalis genotyping not linked to clinical symptomatology and nutritional status of school-aged children of Soledad and Galapa municipality schools, Atlántico, Colombia |
| dc.creator.fl_str_mv |
Muñoz Salas, Karen Esther Polo Barrios, Alma Maestre Gonzalez, Carolina Rodríguez Macias, Juan Vargas-Zapata, Carmiña Lucía |
| dc.contributor.author.spa.fl_str_mv |
Muñoz Salas, Karen Esther Polo Barrios, Alma Maestre Gonzalez, Carolina Rodríguez Macias, Juan Vargas-Zapata, Carmiña Lucía |
| dc.subject.proposal.eng.fl_str_mv |
Giardia duodenalis Genotypes Children Giardiasis Malnutrition Clinical manifestations |
| topic |
Giardia duodenalis Genotypes Children Giardiasis Malnutrition Clinical manifestations |
| description |
Giardia duodenalis genotypes A and B have been reported in Colombia. The population consisted of 235 schoolchildren whose ages ranged from 2 to 10 yr of age from the municipalities of Soledad and Galapa in the department of Atlántico, Colombia. Fecal samples were obtained and then analyzed in triplicate using the sedimentation in formalin-ether (Ritchie's method) and direct examination techniques. Of the 235 fecal samples, 35 samples were positive for G. duodenalis; positive samples were concentrated in a sucrose gradient and sonicated for 3 cycles of 20 sec. DNA extraction was performed, and the parasites were genotyped by conventional PCR amplifying a region of the β-giardin gene. A general prevalence of G. duodenalis of 13.2% was found, and of these genotyped samples, 13 (56.7%) and 7 (20%) corresponded to genotype A, 1 (4.3%), and 3 (25%) corresponded to genotype B, and 9 (39.1%) and 2 (16.7%) were not defined, in the municipalities Soledad and Galapa, respectively. Additionally, 23 children were diagnosed with symptomatologic giardiasis, and 12 were asymptomatic; the most relevant symptoms were abdominal pain (7, 20%) and diarrhea (13, 56.7%). The nutritional status of children with Giardia genotypes A and B were as follows: 3 in a state of malnutrition (10%), 10 normal (33.3%), and 6 overweight and obese (20%) with genotype A, and 1 in a state of malnutrition (3.3%) and 3 normal (10%) with genotype B. The genotypes found in G. duodenalis did not show an association with nutritional status or with the clinical manifestations evaluated in schoolchildren. |
| publishDate |
2022 |
| dc.date.accessioned.none.fl_str_mv |
2022-05-10T15:04:09Z |
| dc.date.available.none.fl_str_mv |
2022-05-10T15:04:09Z 2023-01-07 |
| dc.date.issued.none.fl_str_mv |
2022-01-07 |
| dc.type.spa.fl_str_mv |
Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/resource_type/c_6501 |
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Text |
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info:eu-repo/semantics/article |
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http://purl.org/redcol/resource_type/ART |
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info:eu-repo/semantics/acceptedVersion |
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http://purl.org/coar/resource_type/c_6501 |
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acceptedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Karen Muñoz Salas, Alma Polo Barrios, Carolina Maestre Gonzalez, Juan Rodríguez Macias, and Carmiña Vargas Zapata "Giardia duodenalis Genotyping Not Linked to Clinical Symptomatology and Nutritional Status of School-Aged Children of Soledad and Galapa Municipality Schools, Atlántico, Colombia," Journal of Parasitology 108(1), 1-9, (7 January 2022). https://doi.org/10.1645/21-26 |
| dc.identifier.issn.spa.fl_str_mv |
0022-3395 |
| dc.identifier.uri.spa.fl_str_mv |
https://hdl.handle.net/11323/9156 |
| dc.identifier.url.spa.fl_str_mv |
https://doi.org/10.1645/21-26 |
| dc.identifier.doi.spa.fl_str_mv |
10.1645/21-26 |
| dc.identifier.eissn.spa.fl_str_mv |
1937-2345 |
| dc.identifier.instname.spa.fl_str_mv |
Corporación Universidad de la Costa |
| dc.identifier.reponame.spa.fl_str_mv |
REDICUC - Repositorio CUC |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.cuc.edu.co/ |
| identifier_str_mv |
Karen Muñoz Salas, Alma Polo Barrios, Carolina Maestre Gonzalez, Juan Rodríguez Macias, and Carmiña Vargas Zapata "Giardia duodenalis Genotyping Not Linked to Clinical Symptomatology and Nutritional Status of School-Aged Children of Soledad and Galapa Municipality Schools, Atlántico, Colombia," Journal of Parasitology 108(1), 1-9, (7 January 2022). https://doi.org/10.1645/21-26 0022-3395 10.1645/21-26 1937-2345 Corporación Universidad de la Costa REDICUC - Repositorio CUC |
| url |
https://hdl.handle.net/11323/9156 https://doi.org/10.1645/21-26 https://repositorio.cuc.edu.co/ |
| dc.language.iso.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournal.spa.fl_str_mv |
The Journal of Parasitology |
| dc.relation.references.spa.fl_str_mv |
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Muñoz Salas, Karen EstherPolo Barrios, AlmaMaestre Gonzalez, CarolinaRodríguez Macias, JuanVargas-Zapata, Carmiña Lucía2022-05-10T15:04:09Z2023-01-072022-05-10T15:04:09Z2022-01-07Karen Muñoz Salas, Alma Polo Barrios, Carolina Maestre Gonzalez, Juan Rodríguez Macias, and Carmiña Vargas Zapata "Giardia duodenalis Genotyping Not Linked to Clinical Symptomatology and Nutritional Status of School-Aged Children of Soledad and Galapa Municipality Schools, Atlántico, Colombia," Journal of Parasitology 108(1), 1-9, (7 January 2022). https://doi.org/10.1645/21-260022-3395https://hdl.handle.net/11323/9156https://doi.org/10.1645/21-2610.1645/21-261937-2345Corporación Universidad de la CostaREDICUC - Repositorio CUChttps://repositorio.cuc.edu.co/Giardia duodenalis genotypes A and B have been reported in Colombia. The population consisted of 235 schoolchildren whose ages ranged from 2 to 10 yr of age from the municipalities of Soledad and Galapa in the department of Atlántico, Colombia. Fecal samples were obtained and then analyzed in triplicate using the sedimentation in formalin-ether (Ritchie's method) and direct examination techniques. Of the 235 fecal samples, 35 samples were positive for G. duodenalis; positive samples were concentrated in a sucrose gradient and sonicated for 3 cycles of 20 sec. DNA extraction was performed, and the parasites were genotyped by conventional PCR amplifying a region of the β-giardin gene. A general prevalence of G. duodenalis of 13.2% was found, and of these genotyped samples, 13 (56.7%) and 7 (20%) corresponded to genotype A, 1 (4.3%), and 3 (25%) corresponded to genotype B, and 9 (39.1%) and 2 (16.7%) were not defined, in the municipalities Soledad and Galapa, respectively. Additionally, 23 children were diagnosed with symptomatologic giardiasis, and 12 were asymptomatic; the most relevant symptoms were abdominal pain (7, 20%) and diarrhea (13, 56.7%). The nutritional status of children with Giardia genotypes A and B were as follows: 3 in a state of malnutrition (10%), 10 normal (33.3%), and 6 overweight and obese (20%) with genotype A, and 1 in a state of malnutrition (3.3%) and 3 normal (10%) with genotype B. The genotypes found in G. duodenalis did not show an association with nutritional status or with the clinical manifestations evaluated in schoolchildren.9 páginasapplication/pdfengAmerican Society of ParasitologistsUnited StatesAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)© American Society of Parasitologists 2022https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/embargoedAccesshttp://purl.org/coar/access_right/c_f1cfGiardia duodenalis genotyping not linked to clinical symptomatology and nutritional status of school-aged children of Soledad and Galapa municipality schools, Atlántico, ColombiaArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/acceptedVersionhttps://bioone.org/journals/journal-of-parasitology/volume-108/issue-1/21-26/Giardia-duodenalis-Genotyping-Not-Linked-to-Clinical-Symptomatology-and-Nutritional/10.1645/21-26.shortColombiaSoledadGalapaAtlánticoThe Journal of Parasitology1. 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