Effects of methylmercury and retinol palmitate co-administration in rats during pregnancy and breastfeeding: metabolic and redox parameters in dams and their offspring
Ubiquitous low-dose methylmercury (MeHg) exposure through an increased fish consumption represents a global public health problem, especially among pregnant women. A plethora of micronutrients presented in fish affects MeHg uptake/distribution, but limited data is available. Vitamin A (VitA), anothe...
- Autores:
-
Espitia Pérez, Pedro
Albino Marin, Suelen
Da Rosa, Helen Tais
Silveira, Alexandre Kleber
Espitia Pérez, Lyda
Brango, Hugo
Moraes Pompéu, Diogo
Hermann Silva, Paolla Rissi
Mingori Rodrigues, Moara
Barreto, Fabiano
Kunzler, Alice
Gelain, D. P.
Schnorr, Carlos Eduardo
Moreira Fonseca, José Cláudio
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2018
- Institución:
- Corporación Universidad de la Costa
- Repositorio:
- REDICUC - Repositorio CUC
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.cuc.edu.co:11323/3297
- Acceso en línea:
- http://hdl.handle.net/11323/3297
https://repositorio.cuc.edu.co/
- Palabra clave:
- Co-exposure
Methylmercury
Oxidative stress
Pregnancy
Vitamin A.
Coexposición
Metilmercurio
Estrés oxidativo
El embarazo
Vitamina A
- Rights
- openAccess
- License
- Attribution-NonCommercial-ShareAlike 4.0 International
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|
dc.title.spa.fl_str_mv |
Effects of methylmercury and retinol palmitate co-administration in rats during pregnancy and breastfeeding: metabolic and redox parameters in dams and their offspring |
dc.title.translated.spa.fl_str_mv |
Efectos de la coadministración de metilmercurio y palmitato de retinol en ratas durante el embarazo y la lactancia materna: parámetros metabólicos y redox en las madres y sus crías |
title |
Effects of methylmercury and retinol palmitate co-administration in rats during pregnancy and breastfeeding: metabolic and redox parameters in dams and their offspring |
spellingShingle |
Effects of methylmercury and retinol palmitate co-administration in rats during pregnancy and breastfeeding: metabolic and redox parameters in dams and their offspring Co-exposure Methylmercury Oxidative stress Pregnancy Vitamin A. Coexposición Metilmercurio Estrés oxidativo El embarazo Vitamina A |
title_short |
Effects of methylmercury and retinol palmitate co-administration in rats during pregnancy and breastfeeding: metabolic and redox parameters in dams and their offspring |
title_full |
Effects of methylmercury and retinol palmitate co-administration in rats during pregnancy and breastfeeding: metabolic and redox parameters in dams and their offspring |
title_fullStr |
Effects of methylmercury and retinol palmitate co-administration in rats during pregnancy and breastfeeding: metabolic and redox parameters in dams and their offspring |
title_full_unstemmed |
Effects of methylmercury and retinol palmitate co-administration in rats during pregnancy and breastfeeding: metabolic and redox parameters in dams and their offspring |
title_sort |
Effects of methylmercury and retinol palmitate co-administration in rats during pregnancy and breastfeeding: metabolic and redox parameters in dams and their offspring |
dc.creator.fl_str_mv |
Espitia Pérez, Pedro Albino Marin, Suelen Da Rosa, Helen Tais Silveira, Alexandre Kleber Espitia Pérez, Lyda Brango, Hugo Moraes Pompéu, Diogo Hermann Silva, Paolla Rissi Mingori Rodrigues, Moara Barreto, Fabiano Kunzler, Alice Gelain, D. P. Schnorr, Carlos Eduardo Moreira Fonseca, José Cláudio |
dc.contributor.author.spa.fl_str_mv |
Espitia Pérez, Pedro Albino Marin, Suelen Da Rosa, Helen Tais Silveira, Alexandre Kleber Espitia Pérez, Lyda Brango, Hugo Moraes Pompéu, Diogo Hermann Silva, Paolla Rissi Mingori Rodrigues, Moara Barreto, Fabiano Kunzler, Alice Gelain, D. P. Schnorr, Carlos Eduardo Moreira Fonseca, José Cláudio |
dc.subject.spa.fl_str_mv |
Co-exposure Methylmercury Oxidative stress Pregnancy Vitamin A. Coexposición Metilmercurio Estrés oxidativo El embarazo Vitamina A |
topic |
Co-exposure Methylmercury Oxidative stress Pregnancy Vitamin A. Coexposición Metilmercurio Estrés oxidativo El embarazo Vitamina A |
description |
Ubiquitous low-dose methylmercury (MeHg) exposure through an increased fish consumption represents a global public health problem, especially among pregnant women. A plethora of micronutrients presented in fish affects MeHg uptake/distribution, but limited data is available. Vitamin A (VitA), another fish micronutrient is used in nutritional supplementation, especially during pregnancy. However, there is no information about the health effects arising from their combined exposure. Therefore, the present study aimed to examine the effects of both MeHg and retinyl palmitate administered on pregnant and lactating rats in metabolic and redox parameters from dams and their offspring. Thirty Wistar female rats were orally supplemented with MeHg (0,5 mg/kg/day) and retinyl palmitate (7500 µg RAE/kg/day) via gavage, either individually or in combination from the gestational day 0 to weaning. For dams (150 days old) and their offspring (31 days old), glycogen accumulation (hepatic and cardiac) and retinoid contents (plasma and liver) were analyzed. Hg deposition in liver tissue was quantified. Redox parameters (liver, kidney, and heart) were evaluated for both animals. Cytogenetic damage was analyzed with micronucleus test. Our results showed no general toxic or metabolic alterations in dams and their offspring by MeHg-VitA coadministration during pregnancy and lactation. However, increased lipoperoxidation in maternal liver and a disrupted pro-oxidant response in the heart of male pups was encountered, with apparently no particular effects in the antioxidant response in female offspring. GST activity in dam kidney was altered leading to possible redox disruption of this tissue with no alterations in offspring. Finally, the genomic damage was exacerbated in both male and female pups. In conclusion, low-dose MeHg exposure and retinyl palmitate supplementation during gestation and lactation produced a potentiated pro-oxidant effect, which was tissue-specific. Although this is a pre-clinical approach, we recommend precaution for pregnant women regarding food consumption, and we encourage more epidemiological studies to assess possible modulations effects of MeHg-VitA co- administration at safe or inadvertently used doses in humans, which may be related to specific pathologies in mothers and their children. |
publishDate |
2018 |
dc.date.issued.none.fl_str_mv |
2018-06-29 |
dc.date.accessioned.none.fl_str_mv |
2019-05-10T16:32:33Z |
dc.date.available.none.fl_str_mv |
2019-05-10T16:32:33Z |
dc.type.spa.fl_str_mv |
Artículo de revista |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
format |
http://purl.org/coar/resource_type/c_6501 |
status_str |
acceptedVersion |
dc.identifier.issn.spa.fl_str_mv |
01476513 |
dc.identifier.uri.spa.fl_str_mv |
http://hdl.handle.net/11323/3297 |
dc.identifier.instname.spa.fl_str_mv |
Corporación Universidad de la Costa |
dc.identifier.reponame.spa.fl_str_mv |
REDICUC - Repositorio CUC |
dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.cuc.edu.co/ |
identifier_str_mv |
01476513 Corporación Universidad de la Costa REDICUC - Repositorio CUC |
url |
http://hdl.handle.net/11323/3297 https://repositorio.cuc.edu.co/ |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.rights.spa.fl_str_mv |
Attribution-NonCommercial-ShareAlike 4.0 International |
dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ |
dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.coar.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
rights_invalid_str_mv |
Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.publisher.spa.fl_str_mv |
Ecotoxicology And Environmental Safety |
institution |
Corporación Universidad de la Costa |
bitstream.url.fl_str_mv |
https://repositorio.cuc.edu.co/bitstream/11323/3297/1/Effects%20Of%20Methylmercury%20And%20Retinol.pdf https://repositorio.cuc.edu.co/bitstream/11323/3297/2/license_rdf https://repositorio.cuc.edu.co/bitstream/11323/3297/3/license.txt https://repositorio.cuc.edu.co/bitstream/11323/3297/5/Effects%20Of%20Methylmercury%20And%20Retinol.pdf.jpg https://repositorio.cuc.edu.co/bitstream/11323/3297/6/Effects%20Of%20Methylmercury%20And%20Retinol.pdf.txt |
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Espitia Pérez, Pedro7493f862c003a73504e42525a648f4f9Albino Marin, Suelen69084095956beab948675d33fd5f5df5Da Rosa, Helen Tais7646787f57738099ed1b4c3fa1791429Silveira, Alexandre Kleberd086e2e46934efe7c180e71e7250c3d3Espitia Pérez, Lyda50db36b3a0cc5aebf6056539b85bb0f8Brango, Hugode1c3e1df5cd8f3d1a7689684e8bfb90Moraes Pompéu, Diogo01861f14153cd51512623f6cde0a08edHermann Silva, Paolla Rissif2ec920ae8ca66f85e0092ed87d510d7Mingori Rodrigues, Moaraa1d63c1b45d228d872783e89ae71ff17Barreto, Fabiano7992f7389dd01c6c3d6339ae2b69d0edKunzler, Alice7c1ece46d9dd8d15d54ef53804550d1fGelain, D. P.9bc29c2da362060dd3f660f078c753feSchnorr, Carlos Eduardo2b8ae605be739d729c766b18710a9e6dMoreira Fonseca, José Cláudio96a153a3498a5bbba33615963697ac712019-05-10T16:32:33Z2019-05-10T16:32:33Z2018-06-2901476513http://hdl.handle.net/11323/3297Corporación Universidad de la CostaREDICUC - Repositorio CUChttps://repositorio.cuc.edu.co/Ubiquitous low-dose methylmercury (MeHg) exposure through an increased fish consumption represents a global public health problem, especially among pregnant women. A plethora of micronutrients presented in fish affects MeHg uptake/distribution, but limited data is available. Vitamin A (VitA), another fish micronutrient is used in nutritional supplementation, especially during pregnancy. However, there is no information about the health effects arising from their combined exposure. Therefore, the present study aimed to examine the effects of both MeHg and retinyl palmitate administered on pregnant and lactating rats in metabolic and redox parameters from dams and their offspring. Thirty Wistar female rats were orally supplemented with MeHg (0,5 mg/kg/day) and retinyl palmitate (7500 µg RAE/kg/day) via gavage, either individually or in combination from the gestational day 0 to weaning. For dams (150 days old) and their offspring (31 days old), glycogen accumulation (hepatic and cardiac) and retinoid contents (plasma and liver) were analyzed. Hg deposition in liver tissue was quantified. Redox parameters (liver, kidney, and heart) were evaluated for both animals. Cytogenetic damage was analyzed with micronucleus test. Our results showed no general toxic or metabolic alterations in dams and their offspring by MeHg-VitA coadministration during pregnancy and lactation. However, increased lipoperoxidation in maternal liver and a disrupted pro-oxidant response in the heart of male pups was encountered, with apparently no particular effects in the antioxidant response in female offspring. GST activity in dam kidney was altered leading to possible redox disruption of this tissue with no alterations in offspring. Finally, the genomic damage was exacerbated in both male and female pups. In conclusion, low-dose MeHg exposure and retinyl palmitate supplementation during gestation and lactation produced a potentiated pro-oxidant effect, which was tissue-specific. Although this is a pre-clinical approach, we recommend precaution for pregnant women regarding food consumption, and we encourage more epidemiological studies to assess possible modulations effects of MeHg-VitA co- administration at safe or inadvertently used doses in humans, which may be related to specific pathologies in mothers and their children.Exposición ubicua a baja dosis de metilmercurio (mehg) a través de un aumento de los peces el consumo representa un problema de salud pública mundial, especialmente entre las embarazadas mujeres. Una gran cantidad de micronutrientes presentados en pescado afecta mehg captación / distribución, pero se dispone de datos limitados. Vitamina a (vita), otro pescado. El micronutriente se usa en suplementos nutricionales, especialmente durante el embarazo. Sin embargo, no hay información sobre los efectos en la salud que surgen de su exposición combinada. Por lo tanto, el presente estudio tuvo como objetivo examinar los efectos de mehg y palmitato de retinilo administrados en ratas gestantes y lactantes en parámetros metabólicos y redox de las represas y su descendencia. Treinta wistar ratas hembra fueron suplementadas oralmente con mehg (0,5 mg / kg / día) y retinilo palmitato (7500 µg rae / kg / día) por sonda, individualmente o en combinación desde el día gestacional 0 hasta el destete. Para las presas (150 días de antigüedad) y sus crías. (31 días de edad), acumulación de glucógeno (hepático y cardíaco) y retinoides. (plasma e hígado) fueron analizados. Se cuantificó la deposición de hg en tejido hepático. Se evaluaron los parámetros redox (hígado, riñón y corazón) para ambos animales. El daño citogenético se analizó con prueba de micronúcleo. Nuestros resultados no mostraron alteraciones generales tóxicas o metabólicas en las madres y su descendencia por coadministración de mehg-vita durante el embarazo y la lactancia. Sin embargo, aumentó lipoperoxidación en el hígado materno y una respuesta prooxidante interrumpida en el corazón. De cachorros machos, aparentemente sin efectos particulares en la respuesta antioxidante en la descendencia femenina. Se alteró la actividad de gst en riñón de la presa. Llevando a una posible alteración redox de este tejido sin alteraciones en la descendencia. Finalmente, el daño genómico se exacerbó tanto en cachorros machos como hembras. En conclusión, la exposición a baja dosis de mehg y la suplementación con palmitato de retinilo durante la gestación y la lactancia produjeron un efecto prooxidante potenciado, que fue tejido específico, aunque este es un enfoque preclínico, recomendamos precaución. Para las mujeres embarazadas en relación con el consumo de alimentos, y animamos estudios epidemiológicos para evaluar los posibles efectos moduladores de la co-mehg-vita la administración a dosis seguras o inadvertidamente utilizadas en humanos, que pueden ser relacionados con patologías específicas en madres y sus hijosengEcotoxicology And Environmental SafetyAttribution-NonCommercial-ShareAlike 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Co-exposureMethylmercuryOxidative stressPregnancyVitamin A.CoexposiciónMetilmercurioEstrés oxidativoEl embarazoVitamina AEffects of methylmercury and retinol palmitate co-administration in rats during pregnancy and breastfeeding: metabolic and redox parameters in dams and their offspringEfectos de la coadministración de metilmercurio y palmitato de retinol en ratas durante el embarazo y la lactancia materna: parámetros metabólicos y redox en las madres y sus críasArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/acceptedVersionORIGINALEffects Of Methylmercury And Retinol.pdfEffects Of Methylmercury And Retinol.pdfapplication/pdf183970https://repositorio.cuc.edu.co/bitstream/11323/3297/1/Effects%20Of%20Methylmercury%20And%20Retinol.pdf22e5c399b70739e4be5a44d73f4b6b30MD51open accessCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81031https://repositorio.cuc.edu.co/bitstream/11323/3297/2/license_rdf934f4ca17e109e0a05eaeaba504d7ce4MD52open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.cuc.edu.co/bitstream/11323/3297/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53open accessTHUMBNAILEffects Of Methylmercury And Retinol.pdf.jpgEffects Of Methylmercury And Retinol.pdf.jpgimage/jpeg64353https://repositorio.cuc.edu.co/bitstream/11323/3297/5/Effects%20Of%20Methylmercury%20And%20Retinol.pdf.jpgc9dab31b75e238e35c84d11cc07d9fafMD55open accessTEXTEffects Of Methylmercury And Retinol.pdf.txtEffects Of Methylmercury And Retinol.pdf.txttext/plain2974https://repositorio.cuc.edu.co/bitstream/11323/3297/6/Effects%20Of%20Methylmercury%20And%20Retinol.pdf.txt4b463f16e84cd94c9059df6c60e683e6MD56open access11323/3297oai:repositorio.cuc.edu.co:11323/32972023-12-14 14:59:28.005Attribution-NonCommercial-ShareAlike 4.0 International|||http://creativecommons.org/licenses/by-nc-sa/4.0/open accessRepositorio Universidad de La Costabdigital@metabiblioteca.comTk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo= |