Immobilized enzyme reactors based on nucleoside phosphorylases and 2′-deoxyribosyltransferase for the in-flow synthesis of pharmaceutically relevant nucleoside analogues

In this work, a mono- and a bi-enzymatic analytical immobilized enzyme reactors (IMERs) were developed as prototypes for biosynthetic purposes and their performances in the in-flow synthesis of nucleoside analogues of pharmaceutical interest were evaluated. Two biocatalytic routes based on nucleoside 2′-deoxyribosyltransferase from Lactobacillus reuteri (LrNDT) and uridine phosphorylase from Clostridium perfrigens (CpUP)/purine nucleoside phosphorylase from Aeromonas hydrophila (AhPNP) were investigated in the synthesis of 2′-deoxy, 2′,3′-dideoxy and arabinonucleoside derivatives. LrNDT-IMER catalyzed the synthesis of 5-fluoro-2′-deoxyuridine and 5-iodo-2′-deoxyuridine in 65–59% conversion yield, while CpUP/AhPNP-IMER provided the best results for the preparation of arabinosyladenine (60% conversion yield). Both IMERs proved to be promising alternatives to chemical routes for the synthesis of nucleoside analogues. The developed in-flow system represents a powerful tool for the fast production on analytical scale of nucleosides for preliminary biological tests.

• Autores: Rinaldia, Francesca; Fernández-Lucas, Jesús; de la Fuente, Diego; Zheng, Changping; Teodora Bavaro; BAVARO, TEODORA; Peters, Benjamin; MASSOLINI, GABRIELLA; Contig, Paola; de la Mata, Isabel; TERRENI, MARCO; Calleri, Enrica
• Tipo de recurso: http://purl.org/coar/resource_type/c_816b
• Fecha de publicación: 2020
• Institución: Corporación Universidad de la Costa
• Repositorio: REDICUC - Repositorio CUC
• Idioma: eng
• OAI Identifier: oai:repositorio.cuc.edu.co:11323/6247
• Acceso en línea: https://hdl.handle.net/11323/6247; https://doi.org/10.1016/j.biortech.2020.123258; https://repositorio.cuc.edu.co/
• Palabra clave: Biocatalysis; Immobilized enzyme reactors; Nucleoside analogues; Nucleoside 2′-deoxyribosyltransferases; Nucleoside phosphorylases
• Rights: openAccess
• License: CC0 1.0 Universal