Acute exposure to C60 fullerene damages pulmonary mitochondrial function and mechanics
C60 fullerene (C60) nanoparticles, a nanomaterial widely used in technology, can offer risks to humans, overcome biological barriers, and deposit onto the lungs. However, data on its putative pulmonary burden are scanty. Recently, the C60 interaction with mitochondria has been described in vitro and...
- Autores:
-
Fernandes Caldeira, Dayene de Assis
Muniz Mesquita, Flávia
Gomes Pinheiro, Felipe
Ferreira Oliveira, Dahienne
Silva Oliveira, Luis Felipe
Matheus Nascimento, Jose Hamilton
Maeda Takiya, Christina
Maciel, Leonardo
Araujo Zin, Walter
- Tipo de recurso:
- http://purl.org/coar/resource_type/c_816b
- Fecha de publicación:
- 2020
- Institución:
- Corporación Universidad de la Costa
- Repositorio:
- REDICUC - Repositorio CUC
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.cuc.edu.co:11323/7648
- Acceso en línea:
- https://hdl.handle.net/11323/7648
https://doi.org/10.1080/17435390.2020.1863498
https://repositorio.cuc.edu.co/
- Palabra clave:
- Fullerene C60
Lung mechanics
Alveolar collapse
Mitochondrial function
ATP production
Reactive oxygen species
- Rights
- openAccess
- License
- CC0 1.0 Universal
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|
dc.title.spa.fl_str_mv |
Acute exposure to C60 fullerene damages pulmonary mitochondrial function and mechanics |
title |
Acute exposure to C60 fullerene damages pulmonary mitochondrial function and mechanics |
spellingShingle |
Acute exposure to C60 fullerene damages pulmonary mitochondrial function and mechanics Fullerene C60 Lung mechanics Alveolar collapse Mitochondrial function ATP production Reactive oxygen species |
title_short |
Acute exposure to C60 fullerene damages pulmonary mitochondrial function and mechanics |
title_full |
Acute exposure to C60 fullerene damages pulmonary mitochondrial function and mechanics |
title_fullStr |
Acute exposure to C60 fullerene damages pulmonary mitochondrial function and mechanics |
title_full_unstemmed |
Acute exposure to C60 fullerene damages pulmonary mitochondrial function and mechanics |
title_sort |
Acute exposure to C60 fullerene damages pulmonary mitochondrial function and mechanics |
dc.creator.fl_str_mv |
Fernandes Caldeira, Dayene de Assis Muniz Mesquita, Flávia Gomes Pinheiro, Felipe Ferreira Oliveira, Dahienne Silva Oliveira, Luis Felipe Matheus Nascimento, Jose Hamilton Maeda Takiya, Christina Maciel, Leonardo Araujo Zin, Walter |
dc.contributor.author.spa.fl_str_mv |
Fernandes Caldeira, Dayene de Assis Muniz Mesquita, Flávia Gomes Pinheiro, Felipe Ferreira Oliveira, Dahienne Silva Oliveira, Luis Felipe Matheus Nascimento, Jose Hamilton Maeda Takiya, Christina Maciel, Leonardo Araujo Zin, Walter |
dc.subject.spa.fl_str_mv |
Fullerene C60 Lung mechanics Alveolar collapse Mitochondrial function ATP production Reactive oxygen species |
topic |
Fullerene C60 Lung mechanics Alveolar collapse Mitochondrial function ATP production Reactive oxygen species |
description |
C60 fullerene (C60) nanoparticles, a nanomaterial widely used in technology, can offer risks to humans, overcome biological barriers, and deposit onto the lungs. However, data on its putative pulmonary burden are scanty. Recently, the C60 interaction with mitochondria has been described in vitro and in vivo. We hypothesized that C60 impairs lung mechanics and mitochondrial function. Thirty-five male BALB/c mice were randomly divided into two groups intratracheally instilled with vehicle (0.9% NaCl + 1% Tween 80, CTRL) or C60 (1.0 mg/kg, FUL). Twenty-four hours after exposure, 15 FUL and 8 CTRL mice were anesthetized, paralyzed, and mechanically ventilated for the determination of lung mechanics. After euthanasia, the lungs were removed en bloc at end-expiration for histological processing. Lung tissue elastance and viscance were augmented in FUL group. Increased inflammatory cell number, alveolar collapse, septal thickening, and pulmonary edema were detected. In other six FUL and six CTRL mice, mitochondria expressed reduction in state 1 respiration [FUL = 3.0 ± 1.14 vs. CTRL = 4.46 ± 0.9 (SEM) nmol O2/min/mg protein, p = 0.0210], ATP production (FUL = 122.6 ± 18 vs. CTRL = 154.5 ± 14 μmol/100 μg protein, p = 0.0340), and higher oxygen consumption in state 4 [FUL = 12.56 ± 0.9 vs. CTRL = 8.26 ± 0.6], generation of reactive oxygen species (FUL 733.1 ± 169.32 vs. CTRL = 486.39 ± 73.1 nmol/100 μg protein, p = 0.0313) and reason ROS/ATP [FUL = 8.73 ± 2.3 vs. CTRL = 2.99 ± 0.3]. In conclusion, exposure to fullerene C60 impaired pulmonary mechanics and mitochondrial function, increased ROS concentration, and decrease ATP production. |
publishDate |
2020 |
dc.date.accessioned.none.fl_str_mv |
2020-12-29T20:09:27Z |
dc.date.available.none.fl_str_mv |
2020-12-29T20:09:27Z |
dc.date.issued.none.fl_str_mv |
2020-11-28 |
dc.type.spa.fl_str_mv |
Pre-Publicación |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_816b |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/preprint |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ARTOTR |
dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
format |
http://purl.org/coar/resource_type/c_816b |
status_str |
acceptedVersion |
dc.identifier.uri.spa.fl_str_mv |
https://hdl.handle.net/11323/7648 |
dc.identifier.doi.spa.fl_str_mv |
https://doi.org/10.1080/17435390.2020.1863498 |
dc.identifier.instname.spa.fl_str_mv |
Corporación Universidad de la Costa |
dc.identifier.reponame.spa.fl_str_mv |
REDICUC - Repositorio CUC |
dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.cuc.edu.co/ |
url |
https://hdl.handle.net/11323/7648 https://doi.org/10.1080/17435390.2020.1863498 https://repositorio.cuc.edu.co/ |
identifier_str_mv |
Corporación Universidad de la Costa REDICUC - Repositorio CUC |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.rights.spa.fl_str_mv |
CC0 1.0 Universal |
dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/publicdomain/zero/1.0/ |
dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.coar.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
rights_invalid_str_mv |
CC0 1.0 Universal http://creativecommons.org/publicdomain/zero/1.0/ http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Corporación Universidad de la Costa |
dc.source.spa.fl_str_mv |
Nanotoxicology |
institution |
Corporación Universidad de la Costa |
dc.source.url.spa.fl_str_mv |
https://www.tandfonline.com/doi/full/10.1080/17435390.2020.1863498 |
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Fernandes Caldeira, Dayene de AssisMuniz Mesquita, FláviaGomes Pinheiro, FelipeFerreira Oliveira, DahienneSilva Oliveira, Luis FelipeMatheus Nascimento, Jose HamiltonMaeda Takiya, ChristinaMaciel, LeonardoAraujo Zin, Walter2020-12-29T20:09:27Z2020-12-29T20:09:27Z2020-11-28https://hdl.handle.net/11323/7648https://doi.org/10.1080/17435390.2020.1863498Corporación Universidad de la CostaREDICUC - Repositorio CUChttps://repositorio.cuc.edu.co/C60 fullerene (C60) nanoparticles, a nanomaterial widely used in technology, can offer risks to humans, overcome biological barriers, and deposit onto the lungs. However, data on its putative pulmonary burden are scanty. Recently, the C60 interaction with mitochondria has been described in vitro and in vivo. We hypothesized that C60 impairs lung mechanics and mitochondrial function. Thirty-five male BALB/c mice were randomly divided into two groups intratracheally instilled with vehicle (0.9% NaCl + 1% Tween 80, CTRL) or C60 (1.0 mg/kg, FUL). Twenty-four hours after exposure, 15 FUL and 8 CTRL mice were anesthetized, paralyzed, and mechanically ventilated for the determination of lung mechanics. After euthanasia, the lungs were removed en bloc at end-expiration for histological processing. Lung tissue elastance and viscance were augmented in FUL group. Increased inflammatory cell number, alveolar collapse, septal thickening, and pulmonary edema were detected. In other six FUL and six CTRL mice, mitochondria expressed reduction in state 1 respiration [FUL = 3.0 ± 1.14 vs. CTRL = 4.46 ± 0.9 (SEM) nmol O2/min/mg protein, p = 0.0210], ATP production (FUL = 122.6 ± 18 vs. CTRL = 154.5 ± 14 μmol/100 μg protein, p = 0.0340), and higher oxygen consumption in state 4 [FUL = 12.56 ± 0.9 vs. CTRL = 8.26 ± 0.6], generation of reactive oxygen species (FUL 733.1 ± 169.32 vs. CTRL = 486.39 ± 73.1 nmol/100 μg protein, p = 0.0313) and reason ROS/ATP [FUL = 8.73 ± 2.3 vs. CTRL = 2.99 ± 0.3]. In conclusion, exposure to fullerene C60 impaired pulmonary mechanics and mitochondrial function, increased ROS concentration, and decrease ATP production.Fernandes Caldeira, Dayene de AssisMuniz Mesquita, FláviaGomes Pinheiro, FelipeFerreira Oliveira, DahienneSilva Oliveira, Luis FelipeMatheus Nascimento, Jose HamiltonMaeda Takiya, ChristinaMaciel, LeonardoAraujo Zin, Walterapplication/pdfengCorporación Universidad de la CostaCC0 1.0 Universalhttp://creativecommons.org/publicdomain/zero/1.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Nanotoxicologyhttps://www.tandfonline.com/doi/full/10.1080/17435390.2020.1863498Fullerene C60Lung mechanicsAlveolar collapseMitochondrial functionATP productionReactive oxygen speciesAcute exposure to C60 fullerene damages pulmonary mitochondrial function and 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