Green synthesis of zinc oxide nanoparticles from sida acuta leaf extract for antibacterial and antioxidant applications, and catalytic degradation of dye through the use of convolutional neural network
This study synthesized zinc oxide nanoparticles (ZnO NPs) using a novel green approach, with Sida acuta leaf extract as a capping and reducing agent to initiate nucleation and structure formation. The innovation of this study lies in demonstrating the originality of utilizing zinc oxide nanoparticle...
- Autores:
-
Jiang, Du
Arwa AL-Huqail
Yan, Cao
Yiding, Sun
Mazen, Garaleh
Ehab El Sayed Massoud
Elimam, Ali
Hamid, Assilzadeh
Escorcia Gutierrez, José
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2024
- Institución:
- Corporación Universidad de la Costa
- Repositorio:
- REDICUC - Repositorio CUC
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.cuc.edu.co:11323/13667
- Acceso en línea:
- https://hdl.handle.net/11323/13667
https://doi.org/10.1016/j.envres.2024.119204
https://repositorio.cuc.edu.co/
- Palabra clave:
- Zinc oxide NPs synthesis
Sida acuta
Antibacterial efficacy
Antioxidant potential
Antibacterial activity
Catalytic dye degradation
Convolutional neural network (CNN)
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
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oai_identifier_str |
oai:repositorio.cuc.edu.co:11323/13667 |
network_acronym_str |
RCUC2 |
network_name_str |
REDICUC - Repositorio CUC |
repository_id_str |
|
dc.title.eng.fl_str_mv |
Green synthesis of zinc oxide nanoparticles from sida acuta leaf extract for antibacterial and antioxidant applications, and catalytic degradation of dye through the use of convolutional neural network |
title |
Green synthesis of zinc oxide nanoparticles from sida acuta leaf extract for antibacterial and antioxidant applications, and catalytic degradation of dye through the use of convolutional neural network |
spellingShingle |
Green synthesis of zinc oxide nanoparticles from sida acuta leaf extract for antibacterial and antioxidant applications, and catalytic degradation of dye through the use of convolutional neural network Zinc oxide NPs synthesis Sida acuta Antibacterial efficacy Antioxidant potential Antibacterial activity Catalytic dye degradation Convolutional neural network (CNN) |
title_short |
Green synthesis of zinc oxide nanoparticles from sida acuta leaf extract for antibacterial and antioxidant applications, and catalytic degradation of dye through the use of convolutional neural network |
title_full |
Green synthesis of zinc oxide nanoparticles from sida acuta leaf extract for antibacterial and antioxidant applications, and catalytic degradation of dye through the use of convolutional neural network |
title_fullStr |
Green synthesis of zinc oxide nanoparticles from sida acuta leaf extract for antibacterial and antioxidant applications, and catalytic degradation of dye through the use of convolutional neural network |
title_full_unstemmed |
Green synthesis of zinc oxide nanoparticles from sida acuta leaf extract for antibacterial and antioxidant applications, and catalytic degradation of dye through the use of convolutional neural network |
title_sort |
Green synthesis of zinc oxide nanoparticles from sida acuta leaf extract for antibacterial and antioxidant applications, and catalytic degradation of dye through the use of convolutional neural network |
dc.creator.fl_str_mv |
Jiang, Du Arwa AL-Huqail Yan, Cao Yiding, Sun Mazen, Garaleh Ehab El Sayed Massoud Elimam, Ali Hamid, Assilzadeh Escorcia Gutierrez, José |
dc.contributor.author.none.fl_str_mv |
Jiang, Du Arwa AL-Huqail Yan, Cao Yiding, Sun Mazen, Garaleh Ehab El Sayed Massoud Elimam, Ali Hamid, Assilzadeh Escorcia Gutierrez, José |
dc.subject.proposal.eng.fl_str_mv |
Zinc oxide NPs synthesis Sida acuta Antibacterial efficacy Antioxidant potential Antibacterial activity Catalytic dye degradation Convolutional neural network (CNN) |
topic |
Zinc oxide NPs synthesis Sida acuta Antibacterial efficacy Antioxidant potential Antibacterial activity Catalytic dye degradation Convolutional neural network (CNN) |
description |
This study synthesized zinc oxide nanoparticles (ZnO NPs) using a novel green approach, with Sida acuta leaf extract as a capping and reducing agent to initiate nucleation and structure formation. The innovation of this study lies in demonstrating the originality of utilizing zinc oxide nanoparticles for antibacterial action, antioxidant potential, and catalytic degradation of Congo red dye. This unique approach harnesses eco-friendly methods to initiate nucleation and structure formation. The synthesized nanoparticles' structure and conformation were characterized using UV–vis (λmax = 280 nm), X-ray, atomic force microscopy, SEM, HR-TEM and FTIR. The antibacterial activity of the Nps was tested against Pseudomonas sp, Klebsiella sp, Staphylococcus aureus, and E. coli, demonstrating efficacy. The nanoparticles exhibited unique properties, with a crystallite size of 20 nm (XRD), a surface roughness of 2.5 nm (AFM), and a specific surface area of 60 m2/g (SEM). A Convolutional Neural Network (CNN) was effectively employed to accurately classify and analyze microscopic images of green-synthesized zinc oxide nanoparticles. This research revealed their exceptional antioxidant potential, with an average DPPH scavenging rate of 80% at a concentration of 0.05 mg/mL. Additionally, zeta potential measurements indicated a stable net negative surface charge of approximately −12.2 mV. These quantitative findings highlight the promising applications of green-synthesized ZnO NPs in healthcare, materials science, and environmental remediation. The ZnO nanoparticles exhibited catalytic capabilities for dye degradation, and the degradation rate was determined using UV spectroscopy. Key findings of the study encompass the green synthesis of versatile zinc oxide nanoparticles, demonstrating potent antibacterial action, antioxidant capabilities, and catalytic dye degradation potential. These nanoparticles offer multifaceted solutions with minimal environmental impact, addressing challenges in various fields, from healthcare to environmental remediation. |
publishDate |
2024 |
dc.date.accessioned.none.fl_str_mv |
2024-11-12T13:00:13Z |
dc.date.available.none.fl_str_mv |
2024-11-12T13:00:13Z |
dc.date.issued.none.fl_str_mv |
2024-05-25 |
dc.type.none.fl_str_mv |
Artículo de revista |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.content.none.fl_str_mv |
Text |
dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.redcol.none.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
dc.type.version.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coarversion.none.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
status_str |
publishedVersion |
dc.identifier.citation.none.fl_str_mv |
Jiang Du, Arwa AL-Huqail, Yan Cao, Hui Yao, Yiding Sun, Mazen Garaleh, Ehab El Sayed Massoud, Elimam Ali, Hamid Assilzadeh, José Escorcia-Gutierrez, Green synthesis of zinc oxide nanoparticles from Sida acuta leaf extract for antibacterial and antioxidant applications, and catalytic degradation of dye through the use of convolutional neural network, Environmental Research, Volume 258, 2024, 119204, ISSN 0013-9351, https://doi.org/10.1016/j.envres.2024.119204. |
dc.identifier.issn.none.fl_str_mv |
0013-9351 |
dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/11323/13667 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.envres.2024.119204 |
dc.identifier.eissn.none.fl_str_mv |
1096-0953 |
dc.identifier.instname.none.fl_str_mv |
Corporación Universidad de la Costa |
dc.identifier.reponame.none.fl_str_mv |
REDICUC - Repositorio CUC |
dc.identifier.repourl.none.fl_str_mv |
https://repositorio.cuc.edu.co/ |
identifier_str_mv |
Jiang Du, Arwa AL-Huqail, Yan Cao, Hui Yao, Yiding Sun, Mazen Garaleh, Ehab El Sayed Massoud, Elimam Ali, Hamid Assilzadeh, José Escorcia-Gutierrez, Green synthesis of zinc oxide nanoparticles from Sida acuta leaf extract for antibacterial and antioxidant applications, and catalytic degradation of dye through the use of convolutional neural network, Environmental Research, Volume 258, 2024, 119204, ISSN 0013-9351, https://doi.org/10.1016/j.envres.2024.119204. 0013-9351 1096-0953 Corporación Universidad de la Costa REDICUC - Repositorio CUC |
url |
https://hdl.handle.net/11323/13667 https://doi.org/10.1016/j.envres.2024.119204 https://repositorio.cuc.edu.co/ |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofjournal.none.fl_str_mv |
Environmental Research |
dc.relation.references.none.fl_str_mv |
Ab Aziz, S.A.B., et al., 2013. Effect of zeta potential of stanum oxide (SnO2) on electrophoretic deposition (EPD) on porous alumina. Adv. Mater. Res. 795, 334–337. Abbasi, M., Gholizadeh, R., Kasaee, S.R., Vaez, A., Chelliapan, S., Fadhil Al-Qaim, F., Kamyab, H., 2023. An intriguing approach toward antibacterial activity of green synthesized Rutin-templated mesoporous silica nanoparticles decorated with nanosilver. Scientific reports 13 (1), 5987. Abdullah, A.H., Ridha, S., Mohshim, D.F., Yusuf, M., Kamyab, H., Krishna, S., Maoinser, M.A., 2022. A comprehensive review of nanoparticles: Effect on water- based drilling fluids and wellbore stability. Chemosphere 308, 136274. Alwan, R.M., et al., 2015. Synthesis of zinc oXide nanoparticles via sol–gel route and their characterization. Nanosci. Nanotechnol. 5 (1), 1–6. Alyamani, A.A., et al., 2021. Green fabrication of zinc oXide nanoparticles using phlomis leaf extract: characterization and in vitro evaluation of cytotoXicity and antibacterial properties. Molecules 26 (20), 6140. Asmatulu, R., Khan, W., 2019. Chapter 13-Characterization of electrospun nanofibers. Synthesis and Applications of Electrospun Nanofibers 257–281. Balaraman, P., Balasubramanian, B., Kaliannan, D., Durai, M., Kamyab, H., Park, S., Maruthupandian, A., 2020. Phyco-synthesis of silver nanoparticles mediated from marine algae Sargassum myriocystum and its potential biological and environmental applications. Waste and Biomass Valorization 11, 5255–5271. Balaraman, P., Balasubramanian, B., Liu, W.C., Kaliannan, D., Durai, M., Kamyab, H., Maruthupandian, A., 2022. Sargassum myriocystum-mediated TiO2-nanoparticles and their antimicrobial, larvicidal activities and enhanced photocatalytic degradation of various dyes. Environmental research 204, 112278. Bharadwaj, K.K., et al., 2021. Green synthesis of silver nanoparticles using Diospyros malabarica fruit extract and assessments of their antimicrobial, anticancer and catalytic reduction of 4-nitrophenol (4-NP). Nanomaterials 11 (8), 1999. Bhuyan, T., et al., 2015. Biosynthesis of zinc oXide nanoparticles from Azadirachta indica for antibacterial and photocatalytic applications. Mater. Sci. Semicond. Process. 32, 55–61. Blanco, E., Shen, H., Ferrari, M., 2015. Principles of nanoparticle design for overcoming biological barriers to drug delivery. Nat. Biotechnol. 33 (9), 941–951. Brand-Williams, W., Cuvelier, M.E., Berset, C., 1995. Use of a free radical method to evaluate antioXidant activity. LWT - Food Sci. Technol. (Lebensmittel-Wissenschaft -Technol.) 28 (1), 25–30. Chahnasir, E.S., Zandi, Y., Shariati, M., Dehghani, E., Toghroli, A., Mohamad, E.T., Khorami, M., 2018. Application of support vector machine with firefly algorithm for investigation of the factors affecting the shear strength of angle shear connectors. Smart Struct. Systems 22 (4), 413–424. Chen, X., et al., 2017. Preparation of ZnO photocatalyst for the efficient and rapid photocatalytic degradation of azo dyes. Nanoscale Res. Lett. 12, 1–10. Chou Chau, Y.-F., et al., 2019. Plasmonic perfect absorber based on metal nanorod arrays connected with veins. Results Phys. 15, 102567. Cioffi, B., et al., 2021. A potential risk assessment tool to monitor pathogens circulation in coastal waters. Environ. Res. 200, 111748. Das, D., et al., 2013. Synthesis of ZnO nanoparticles and evaluation of antioXidant and cytotoXic activity. Colloids Surf. B Biointerfaces 111, 556–560. Dhatwalia, J., et al., 2022. Rubus ellipticus Sm. Fruit extract mediated zinc oXide nanoparticles: a green approach for dye degradation and biomedical applications. Materials 15 (10), 3470. Dizaj, S.M., et al., 2014. Antimicrobial activity of the metals and metal oXide nanoparticles. Mater. Sci. Eng. C 44, 278–284. Edmond, M.B., et al., 1999. Nosocomial bloodstream infections in United States hospitals: a three-year analysis. Clin. Infect. Dis. 29 (2), 239–244. Etienne, O.K., et al., 2021. Chemical characterization, antioXidant and enzyme inhibitory effects of Mitracarpus hirtus extracts. J. Pharmaceut. Biomed. Anal. 194, 113799. Geetha, M.S., Nagabhushana, H., Shivananjaiah, H.N., 2016. Green mediated synthesis and characterization of ZnO nanoparticles using Euphorbia Jatropa latex as reducing agent. J. Sci.: Advanced Materials and Devices 1 (3), 301–310. Gupta, A., et al., 2016. Nanoemulsions: formation, properties and applications. Soft Matter 12 (11), 2826–2841. Hoshyar, N., et al., 2016. The effect of nanoparticle size on in vivo pharmacokinetics and cellular interaction. Nanomedicine 11 (6), 673–692. Hussain, I., et al., 2017. EXogenous application of phytosynthesized nanoceria to alleviate ferulic acid stress in Solanum lycopersicum. Sci. Hortic. 214, 158–164. Jain, D., et al., 2020. Microbial fabrication of zinc oXide nanoparticles and evaluation of their antimicrobial and photocatalytic properties. Front. Chem. 8, 778. Jobin, M.-L., Alves, I.D., 2014. On the importance of electrostatic interactions between cell penetrating peptides and membranes: a pathway toward tumor cell selectivity? Biochimie 107, 154–159. Ju-Nam, Y., Lead, J., 2008. Manufactured nanoparticles and natural aquatic colloids: an overview of their chemical aspects, interactions and potential environmental implications. Sci. Total Environ. 400, 396–414. Kamyab, H., Chelliapan, S., Hayder, G., Yusuf, M., Taheri, M.M., Rezania, S., Nouri, J., 2023. EXploring the potential of layered metal and metal oXide nanomaterials for sustainable water and wastewater treatment: A review of their antimicrobial properties. Chemosphere 139103. Kaningini, G.A., et al., 2021. Green synthesis and characterization of zinc oXide nanoparticles using bush tea (AthriXia phylicoides DC) natural extract: assessment of the synthesis process. F1000Research 10. Katebi, J., Shoaei-parchin, M., Shariati, M., Trung, N.T., Khorami, M., 2020. Developed comparative analysis of metaheuristic optimization algorithms for optimal active control of structures. Engineering with. Computers 36, 1539–1558. Keflie, T.S., Biesalski, H.K., 2021. Micronutrients and bioactive substances: their potential roles in combating COVID-19. Nutrition 84, 111103. Khan, I., Saeed, K., Khan, I., 2019. Nanoparticles: properties, applications and toXicities. Arab. J. Chem. 12 (7), 908–931. Koehler, A., et al., 2024. Prediction of melanin content of Fonsecaea pedrosoi using Fourier transform infrared spectroscopy (FTIR) and chemometrics. Spectrochim. Acta Mol. Biomol. Spectrosc. 310, 123945. Kouhbanani, M.A.J., Mosleh-Shirazi, S., Beheshtkhoo, N., Kasaee, S.R., Nekouian, S., Alshehery, S., Amani, A.M., 2023. Investigation through the antimicrobial activity of electrospun PCL nanofiber mats with green synthesized Ag–Fe nanoparticles. Journal of Drug Delivery Science and Technology 85, 104541. Kumar, P., Kumar, S.V., 2023. Nanopriming of Eleusine coracana seeds using phyto- assisted magnetic nanoparticles (Fe3O4) synthesized from Colocasia esculenta leaves. Biomass Conversion and Biorefinery. Lallo da Silva, B., et al., 2019. Relationship between structure and antimicrobial activity of zinc oXide nanoparticles: an overview. Int. J. Nanomed. 14, 9395–9410. Lan, S., Lin, J., Zheng, N., 2014. Evaluation of the antioXidant activity of Coreopsis tinctoria Nuff. and optimisation of isolation by response surface methodology. Acta Pharm. 64 (3), 369–378. Lei, H., et al., 2023. Enhanced tribocatalytic degradation of organic pollutants by ZnO nanoparticles of high crystallinity. Nanomaterials 13 (1), 46. Look, D.C., 2001. Recent advances in ZnO materials and devices. Mater. Sci. Eng., B 80 (1), 383–387. Ly, N.H., Nguyen, N.B., Tran, H.N., Hoang, T.T.H., Joo, S.W., Vasseghian, Y., Klemeˇs, J. J., 2023. Metal-organic framework nanopesticide carrier for accurate pesticide delivery and decrement of groundwater pollution. J. Clean. Prod. 402, 136809. Mahdi Ismail, S.M., et al., 2023. Characterization of green synthesized of ZnO nanoparticles by using pinus brutia leaves extracts. J. Mol. Struct. 1280, 135063. Manojkumar, U., Kaliannan, D., Srinivasan, V., Balasubramanian, B., Kamyab, H., Mussa, Z.H., Palaninaicker, S., 2023. Green synthesis of zinc oXide nanoparticles using Brassica oleracea var. botrytis leaf extract: Photocatalytic, antimicrobial and larvicidal activity. Chemosphere 323, 138263. Mohammadhassani, M., Nezamabadi-Pour, H., Suhatril, M., Shariati, M., 2013. Identification of a suitable ANN architecture in predicting strain in tie section of concrete deep beams. Struct. Eng. Mech. Int. J. 46 (6), 853–868. Mohammadhassani, M., Nezamabadi-Pour, H., Suhatril, M., Shariati, M., 2014. An evolutionary fuzzy modelling approach and comparison of different methods for shear strength prediction of high-strength concrete beams without stirrups. Smart Struct. Syst. Int. J. 14 (5), 785–809. Mohd Yusof, H., et al., 2019. Microbial synthesis of zinc oXide nanoparticles and their potential application as an antimicrobial agent and a feed supplement in animal industry: a review. J. Anim. Sci. Biotechnol. 10, 1–22. Mohd Yusof, H., et al., 2020. Biosynthesis of zinc oXide nanoparticles by cell-biomass and supernatant of Lactobacillus plantarum TA4 and its antibacterial and biocompatibility properties. Sci. Rep. 10 (1), 19996. Muhammad, W., et al., 2019. Optical, morphological and biological analysis of zinc oXide nanoparticles (ZnO NPs) using. RSC Adv. 9 (51), 29541–29548. Nava, A.R., Daneshian, L., Sarma, H., 2022. Antibiotic resistant genes in the environment-exploring surveillance methods and sustainable remediation strategies of antibiotics and ARGs. Environ. Res. 215, 114212. Nikalje, A.P., 2015. Nanotechnology and its applications in medicine. Med. Chem. 5 (2), 81–89. Nilavukkarasi, M., Vijayakumar, S., Prathipkumar, S., 2020. Capparis zeylanica mediated bio-synthesized ZnO nanoparticles as antimicrobial, photocatalytic and anti-cancer applications. Materials Science for Energy Technologies 3, 335–343. Pavlova, E.L., Zografov, N.N., Simeonova, L.S., 2016. Comparative study on the antioXidant capacities of synthetic influenza inhibitors and ellagic acid in model systems. Biomed. Pharmacother. 83, 755–762. Prashanna Suvaitha, S., et al., 2023. Optical and biological properties of MgO/ZnO nanocomposite derived via eggshell membrane: a bio-waste approach. Bioproc. Biosyst. Eng. 46 (1), 39–51. Preeti, et al., 2020. ZnO quantum dots: broad spectrum microbicidal agent against multidrug resistant pathogens E. coli and C. albicans. Frontiers in Nanotechnology 2, 576342. Pushparaj, K., Liu, W.C., Meyyazhagan, A., Orlacchio, A., Pappusamy, M., Vadivalagan, C., Balasubramanian, B., 2022. Nano-from nature to nurture: A comprehensive review on facets, trends, perspectives and sustainability of nanotechnology in the food sector. Energy 240, 122732. Rad, S.S., Sani, A.M., Mohseni, S., 2019. Biosynthesis, characterization and antimicrobial activities of zinc oXide nanoparticles from leaf extract of Mentha pulegium (L.). Microb. Pathog. 131, 239–245. Rahimi, M.T., et al., 2015. Scolicidal activity of biosynthesized silver nanoparticles against Echinococcus granulosus protoscolices. Int. J. Surg. 19, 128–133. Rahman, A., et al., 2021. Zinc oXide and zinc oXide-based nanostructures: biogenic and phytogenic synthesis, properties and applications. Bioproc. Biosyst. Eng. 44 (7), 1333–1372. Raimondi, F., et al., 2005. Nanoparticles in energy technology: examples from electrochemistry and catalysis. Angew. Chem. Int. Ed. 44 (15), 2190–2209. Rajeshkumar, S., et al., 2022. Degradation of toXic dye and antimicrobial and free radical potential of environmental benign zinc oXide nanoparticles. Bioinorgan. Chem. Appl. 2022, 4513208. Rasouli, K., Rasouli, J., Mohtaram, M.S., Sabbaghi, S., Kamyab, H., Moradi, H., Chelliapan, S., 2023. Biomass-derived activated carbon nanocomposites for cleaner production: a review on aspects of photocatalytic pollutant degradation. J. Clean. Prod. 138181. Reddy, K.M., et al., 2007. Selective toXicity of zinc oXide nanoparticles to prokaryotic and eukaryotic systems. Appl. Phys. Lett. 90 (21). Regiel-Futyra, A., et al., 2015. The quenching effect of chitosan crosslinking on ZnO nanoparticles photocatalytic activity. RSC Adv. 5 (97), 80089–80097. Safa, M., Kachitvichyanukul, V., 2019. Moment rotation prediction of precast beam to column connections using extreme learning machine. Struct. Eng. Mech. Int. J. 70 (5), 639–647. Safa, M., Sari, P.A., Shariati, M., Suhatril, M., Trung, N.T., Wakil, K., Khorami, M., 2020. Development of neuro-fuzzy and neuro-bee predictive models for prediction of the safety factor of eco-protection slopes. Phys. A: Stat. Mech. Appl. 550, 124046. Safa, M., Shariati, M., Ibrahim, Z., Toghroli, A., Baharom, S.B., Nor, N.M., Petkovi´c, D., 2016. Potential of adaptive neuro fuzzy inference system for evaluating the factors affecting steel-concrete composite beam’s shear strength. Steel Compos. Struct. Int. J. 21 (3), 679–688. Salem, W., et al., 2015. Antibacterial activity of silver and zinc nanoparticles against Vibrio cholerae and enterotoXic Escherichia coli. International Journal of Medical Microbiology 305 (1), 85–95. Santhoshkumar, J., Kumar, S.V., Rajeshkumar, S., 2017. Synthesis of zinc oXide nanoparticles using plant leaf extract against urinary tract infection pathogen. Resource-Efficient Technologies 3 (4), 459–465. Sˇebesta, M., et al., 2019. Increased colloidal stability and decreased solubility—sol—gel synthesis of zinc oXide nanoparticles with humic acids. J. Nanosci. Nanotechnol. 19 (5), 3024–3030. Sedghi, Y., Zandi, Y., Shariati, M., Ahmadi, E., Azar, V.M., Toghroli, A., Wakil, K., 2018. Application of ANFIS technique on performance of C and L shaped angle shear connectors. Smart Struc. Systems 22 (3), 335–340. Segets, D., et al., 2009. Analysis of optical absorbance spectra for the determination of ZnO nanoparticle size distribution, solubility, and surface energy. ACS Nano 3 (7), 1703–1710. Senguttuvan, J., Paulsamy, S., Karthika, K., 2014. Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. for in vitro antioXidant activities. Asian Pac. J. Trop. Biomed. 4, S359–S367. Senthilkumar, R., et al., 2018. Phytochemical screening of aqueous leaf extract of Sida acuta burm. F. And its antibacterial activity. Journal of Emerging Technologies and Innovative Research 5 (8), 474–478. Seshadri, V.D., 2021. Zinc oXide nanoparticles from Cassia auriculata flowers showed the potent antimicrobial and in vitro anticancer activity against the osteosarcoma MG- 63 cells. Saudi J. Biol. Sci. 28 (7), 4046–4054. Shariati, M., Mafipour, M.S., Haido, J.H., Yousif, S.T., Toghroli, A., Trung, N.T., Shariati, A., 2020a. Identification of the most influencing parameters on the properties of corroded concrete beams using an Adaptive Neuro-Fuzzy Inference System (ANFIS). Steel Compos. Struct. 34 (1), 155. Shariati, M., Mafipour, M.S., Mehrabi, P., Ahmadi, M., Wakil, K., Trung, N.T., Toghroli, A., 2020b. Prediction of concrete strength in presence of furnace slag and fly ash using Hybrid ANN-GA (Artificial Neural Network-Genetic Algorithm). Smart Structu. Systems Int. J. 25 (2), 183–195. Shariati, M., Mafipour, M.S., Ghahremani, B., Azarhomayun, F., Ahmadi, M., Trung, N. T., Shariati, A., 2022. A novel hybrid extreme learning machine–grey wolf optimizer (ELM-GWO) model to predict compressive strength of concrete with partial replacements for cement. Eng. Comput. 1–23. Shariati, M., Mafipour, M.S., Mehrabi, P., Bahadori, A., Zandi, Y., Salih, M.N., Poi- Ngian, S., 2019. Application of a hybrid artificial neural network-particle swarm optimization (ANN-PSO) model in behavior prediction of channel shear connectors embedded in normal and high-strength concrete. Applied Sci. 9 (24), 5534. Shittu, M., Alagbe, J., 2020. Phyto-nutritional profiles of broom weed (Sida acuta) leaf extract. International Journal on Integrated Education 3 (11), 119–124. Siddheswaran, R., et al., 2013. Fabrication and characterization of a diluted magnetic semiconducting TM co-doped Al: ZnO (TM Co, Ni) thin films by sol–gel spin coating method. Spectrochim. Acta Mol. Biomol. Spectrosc. 106, 118–123. Sidebottom, D.L., 2024. Dynamic light scattering study of the non-exponential α-relaxation in sodium germanate glass melts. J. Non-Cryst. Solids 627, 122819. Silva, G.A., 2004. Introduction to nanotechnology and its applications to medicine. Surg. Neurol. 61 (3), 216–220. Singh, J., et al., 2018. ‘Green’synthesis of metals and their oXide nanoparticles: applications for environmental remediation. J. Nanobiotechnol. 16 (1), 1–24. Singh, A., et al., 2020. Green synthesis of metallic nanoparticles as effective alternatives to treat antibiotics resistant bacterial infections: a review. Biotechnology Reports 25, e00427. Sirelkhatim, A., et al., 2015. Review on zinc oxide nanoparticles: antibacterial activity and toxicity mechanism. Nano-Micro Lett. 7, 219–242. Srivastava, V., Gusain, D., Sharma, Y.C., 2013. Synthesis, characterization and application of zinc oxide nanoparticles (n-ZnO). Ceram. Int. 39 (8), 9803–9808. Stoimenov, P.K., et al., 2002. Metal oxide nanoparticles as bactericidal agents. Langmuir 18 (17), 6679–6686. Sultanova, N., et al., 2001. Antioxidant and antimicrobial activities of Tamarix ramosissima. J. Ethnopharmacol. 78 (2), 201–205. Syama, S., et al., 2014. Zinc oxide nanoparticles induced oxidative stress in mouse bone marrow mesenchymal stem cells. Toxicol. Mech. Methods 24 (9), 644–653. Tan, Q., et al., 2019. Hierarchical zinc oxide/reduced graphene oxide composite: preparation route, mechanism study and lithium ion storage. J. Colloid Interface Sci. 548, 233–243. Tavakkoli, O., Kamyab, H., Shariati, M., Mohamed, A.M., Junin, R., 2022. Effect of nanoparticles on the performance of polymer/surfactant flooding for enhanced oil recovery: A review. Fuel 312, 122867. Titus, D., James Jebaseelan Samuel, E., Roopan, S.M., 2019. Chapter 12 - nanoparticle characterization techniques. In: Shukla, A.K., Iravani, S. (Eds.), Green Synthesis, Characterization and Applications of Nanoparticles. Elsevier, pp. 303–319. Toghroli, A., Mohammadhassani, M., Suhatril, M., Shariati, M., Ibrahim, Z., 2014. Prediction of shear capacity of channel shear connectors using the ANFIS model. Steel Compos. Struct. 17 (5), 623–639. Veerakumar, K., Govindarajan, M., Rajeswary, M., 2013. Green synthesis of silver nanoparticles using Sida acuta (Malvaceae) leaf extract against Culex quinquefasciatus, Anopheles stephensi, and Aedes aegypti (Diptera: Culicidae). Parasitol. Res. 112 (12), 4073–4085. Verma, C., et al., 2018. An overview on plant extracts as environmental sustainable and green corrosion inhibitors for metals and alloys in aggressive corrosive media. J. Mol. Liq. 266, 577–590. Win, T.T., et al., 2021. Green synthesis and characterization of Fe3O4 nanoparticles using Chlorella-K01 extract for potential enhancement of plant growth stimulating and antifungal activity. Sci. Rep. 11 (1), 21996. Xia, C., et al., 2023. Optimistic and possible contribution of nanomaterial on biomedical applications: a review. Environ. Res. 218, 114921. Xia, C., Ren, T., Darabi, R., Shabani-Nooshabadi, M., Klemeˇs, J.J., Karaman, C., Chelliapan, S., 2023. Spotlighting the boosted energy storage capacity of CoFe2O4/ Graphene nanoribbons: A promising positive electrode material for high-energydensity asymmetric supercapacitor. Energy 270, 126914. Yazdani, M., Kabirifar, K., Frimpong, B.E., Shariati, M., Mirmozaffari, M., Boskabadi, A., 2021. Improving construction and demolition waste collection service in an urban area using a simheuristic approach: A case study in Sydney, Australia. J. Clean. Prod. 280, 124138. Zainah, T.A.S.M.I., Shahaboddin, S.M.S.M.S., 1801. Potential of soft computing approach for evaluating the factors affecting the capacity of steel–concrete composite beam. J. Intell. Manuf. 29 (8), 1793. Zhang, Z., Karimi-Maleh, H., 2023. In situ synthesis of label-free electrochemical aptasensor-based sandwich-like AuNPs/PPy/Ti3C2Tx for ultrasensitive detection of lead ions as hazardous pollutants in environmental fluids. Chemosphere 324, 138302. Zhu, X., Pathakoti, K., Hwang, H.-M., 2019. Chapter 10 - green synthesis of titanium dioxide and zinc oxide nanoparticles and their usage for antimicrobial applications and environmental remediation. In: Shukla, A.K., Iravani, S. (Eds.), Green Synthesis, Characterization and Applications of Nanoparticles. Elsevier, pp. 223–263. |
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© 2024 Published by Elsevier Inc. |
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Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) © 2024 Published by Elsevier Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/ http://purl.org/coar/access_right/c_abf2 |
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28 páginas |
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Academic Press Inc. |
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Academic Press Inc. |
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https://www.sciencedirect.com/science/article/pii/S0013935124011095?via%3Dihub |
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Corporación Universidad de la Costa |
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Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)© 2024 Published by Elsevier Inc.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Jiang, DuArwa AL-HuqailYan, CaoYiding, SunMazen, GaralehEhab El Sayed MassoudElimam, AliHamid, AssilzadehEscorcia Gutierrez, José2024-11-12T13:00:13Z2024-11-12T13:00:13Z2024-05-25Jiang Du, Arwa AL-Huqail, Yan Cao, Hui Yao, Yiding Sun, Mazen Garaleh, Ehab El Sayed Massoud, Elimam Ali, Hamid Assilzadeh, José Escorcia-Gutierrez, Green synthesis of zinc oxide nanoparticles from Sida acuta leaf extract for antibacterial and antioxidant applications, and catalytic degradation of dye through the use of convolutional neural network, Environmental Research, Volume 258, 2024, 119204, ISSN 0013-9351, https://doi.org/10.1016/j.envres.2024.119204.0013-9351https://hdl.handle.net/11323/13667https://doi.org/10.1016/j.envres.2024.1192041096-0953Corporación Universidad de la CostaREDICUC - Repositorio CUChttps://repositorio.cuc.edu.co/This study synthesized zinc oxide nanoparticles (ZnO NPs) using a novel green approach, with Sida acuta leaf extract as a capping and reducing agent to initiate nucleation and structure formation. The innovation of this study lies in demonstrating the originality of utilizing zinc oxide nanoparticles for antibacterial action, antioxidant potential, and catalytic degradation of Congo red dye. This unique approach harnesses eco-friendly methods to initiate nucleation and structure formation. The synthesized nanoparticles' structure and conformation were characterized using UV–vis (λmax = 280 nm), X-ray, atomic force microscopy, SEM, HR-TEM and FTIR. The antibacterial activity of the Nps was tested against Pseudomonas sp, Klebsiella sp, Staphylococcus aureus, and E. coli, demonstrating efficacy. The nanoparticles exhibited unique properties, with a crystallite size of 20 nm (XRD), a surface roughness of 2.5 nm (AFM), and a specific surface area of 60 m2/g (SEM). A Convolutional Neural Network (CNN) was effectively employed to accurately classify and analyze microscopic images of green-synthesized zinc oxide nanoparticles. This research revealed their exceptional antioxidant potential, with an average DPPH scavenging rate of 80% at a concentration of 0.05 mg/mL. Additionally, zeta potential measurements indicated a stable net negative surface charge of approximately −12.2 mV. These quantitative findings highlight the promising applications of green-synthesized ZnO NPs in healthcare, materials science, and environmental remediation. The ZnO nanoparticles exhibited catalytic capabilities for dye degradation, and the degradation rate was determined using UV spectroscopy. Key findings of the study encompass the green synthesis of versatile zinc oxide nanoparticles, demonstrating potent antibacterial action, antioxidant capabilities, and catalytic dye degradation potential. These nanoparticles offer multifaceted solutions with minimal environmental impact, addressing challenges in various fields, from healthcare to environmental remediation.28 páginasapplication/pdfengAcademic Press Inc.United Stateshttps://www.sciencedirect.com/science/article/pii/S0013935124011095?via%3DihubGreen synthesis of zinc oxide nanoparticles from sida acuta leaf extract for antibacterial and antioxidant applications, and catalytic degradation of dye through the use of convolutional neural networkArtículo de revistahttp://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Environmental ResearchAb Aziz, S.A.B., et al., 2013. Effect of zeta potential of stanum oxide (SnO2) on electrophoretic deposition (EPD) on porous alumina. Adv. Mater. Res. 795, 334–337.Abbasi, M., Gholizadeh, R., Kasaee, S.R., Vaez, A., Chelliapan, S., Fadhil Al-Qaim, F., Kamyab, H., 2023. An intriguing approach toward antibacterial activity of green synthesized Rutin-templated mesoporous silica nanoparticles decorated with nanosilver. Scientific reports 13 (1), 5987.Abdullah, A.H., Ridha, S., Mohshim, D.F., Yusuf, M., Kamyab, H., Krishna, S., Maoinser, M.A., 2022. A comprehensive review of nanoparticles: Effect on water- based drilling fluids and wellbore stability. Chemosphere 308, 136274.Alwan, R.M., et al., 2015. Synthesis of zinc oXide nanoparticles via sol–gel route and their characterization. Nanosci. Nanotechnol. 5 (1), 1–6.Alyamani, A.A., et al., 2021. Green fabrication of zinc oXide nanoparticles using phlomis leaf extract: characterization and in vitro evaluation of cytotoXicity and antibacterial properties. Molecules 26 (20), 6140.Asmatulu, R., Khan, W., 2019. Chapter 13-Characterization of electrospun nanofibers. Synthesis and Applications of Electrospun Nanofibers 257–281.Balaraman, P., Balasubramanian, B., Kaliannan, D., Durai, M., Kamyab, H., Park, S., Maruthupandian, A., 2020. Phyco-synthesis of silver nanoparticles mediated from marine algae Sargassum myriocystum and its potential biological and environmental applications. Waste and Biomass Valorization 11, 5255–5271.Balaraman, P., Balasubramanian, B., Liu, W.C., Kaliannan, D., Durai, M., Kamyab, H., Maruthupandian, A., 2022. Sargassum myriocystum-mediated TiO2-nanoparticles and their antimicrobial, larvicidal activities and enhanced photocatalytic degradation of various dyes. Environmental research 204, 112278.Bharadwaj, K.K., et al., 2021. Green synthesis of silver nanoparticles using Diospyros malabarica fruit extract and assessments of their antimicrobial, anticancer and catalytic reduction of 4-nitrophenol (4-NP). Nanomaterials 11 (8), 1999.Bhuyan, T., et al., 2015. Biosynthesis of zinc oXide nanoparticles from Azadirachta indica for antibacterial and photocatalytic applications. Mater. Sci. Semicond. Process. 32, 55–61.Blanco, E., Shen, H., Ferrari, M., 2015. Principles of nanoparticle design for overcoming biological barriers to drug delivery. Nat. Biotechnol. 33 (9), 941–951.Brand-Williams, W., Cuvelier, M.E., Berset, C., 1995. Use of a free radical method to evaluate antioXidant activity. LWT - Food Sci. Technol. (Lebensmittel-Wissenschaft -Technol.) 28 (1), 25–30.Chahnasir, E.S., Zandi, Y., Shariati, M., Dehghani, E., Toghroli, A., Mohamad, E.T., Khorami, M., 2018. Application of support vector machine with firefly algorithm for investigation of the factors affecting the shear strength of angle shear connectors. Smart Struct. Systems 22 (4), 413–424.Chen, X., et al., 2017. Preparation of ZnO photocatalyst for the efficient and rapid photocatalytic degradation of azo dyes. Nanoscale Res. Lett. 12, 1–10.Chou Chau, Y.-F., et al., 2019. Plasmonic perfect absorber based on metal nanorod arrays connected with veins. Results Phys. 15, 102567.Cioffi, B., et al., 2021. A potential risk assessment tool to monitor pathogens circulation in coastal waters. Environ. Res. 200, 111748.Das, D., et al., 2013. Synthesis of ZnO nanoparticles and evaluation of antioXidant and cytotoXic activity. Colloids Surf. B Biointerfaces 111, 556–560.Dhatwalia, J., et al., 2022. Rubus ellipticus Sm. Fruit extract mediated zinc oXide nanoparticles: a green approach for dye degradation and biomedical applications. Materials 15 (10), 3470.Dizaj, S.M., et al., 2014. Antimicrobial activity of the metals and metal oXide nanoparticles. Mater. Sci. Eng. C 44, 278–284.Edmond, M.B., et al., 1999. Nosocomial bloodstream infections in United States hospitals: a three-year analysis. Clin. Infect. Dis. 29 (2), 239–244.Etienne, O.K., et al., 2021. Chemical characterization, antioXidant and enzyme inhibitory effects of Mitracarpus hirtus extracts. J. Pharmaceut. Biomed. Anal. 194, 113799.Geetha, M.S., Nagabhushana, H., Shivananjaiah, H.N., 2016. Green mediated synthesis and characterization of ZnO nanoparticles using Euphorbia Jatropa latex as reducing agent. J. Sci.: Advanced Materials and Devices 1 (3), 301–310.Gupta, A., et al., 2016. Nanoemulsions: formation, properties and applications. Soft Matter 12 (11), 2826–2841.Hoshyar, N., et al., 2016. The effect of nanoparticle size on in vivo pharmacokinetics and cellular interaction. Nanomedicine 11 (6), 673–692.Hussain, I., et al., 2017. EXogenous application of phytosynthesized nanoceria to alleviate ferulic acid stress in Solanum lycopersicum. Sci. Hortic. 214, 158–164.Jain, D., et al., 2020. Microbial fabrication of zinc oXide nanoparticles and evaluation of their antimicrobial and photocatalytic properties. Front. Chem. 8, 778.Jobin, M.-L., Alves, I.D., 2014. On the importance of electrostatic interactions between cell penetrating peptides and membranes: a pathway toward tumor cell selectivity? Biochimie 107, 154–159.Ju-Nam, Y., Lead, J., 2008. Manufactured nanoparticles and natural aquatic colloids: an overview of their chemical aspects, interactions and potential environmental implications. Sci. Total Environ. 400, 396–414.Kamyab, H., Chelliapan, S., Hayder, G., Yusuf, M., Taheri, M.M., Rezania, S., Nouri, J., 2023. EXploring the potential of layered metal and metal oXide nanomaterials for sustainable water and wastewater treatment: A review of their antimicrobial properties. Chemosphere 139103.Kaningini, G.A., et al., 2021. Green synthesis and characterization of zinc oXide nanoparticles using bush tea (AthriXia phylicoides DC) natural extract: assessment of the synthesis process. F1000Research 10.Katebi, J., Shoaei-parchin, M., Shariati, M., Trung, N.T., Khorami, M., 2020. Developed comparative analysis of metaheuristic optimization algorithms for optimal active control of structures. Engineering with. Computers 36, 1539–1558.Keflie, T.S., Biesalski, H.K., 2021. Micronutrients and bioactive substances: their potential roles in combating COVID-19. Nutrition 84, 111103.Khan, I., Saeed, K., Khan, I., 2019. Nanoparticles: properties, applications and toXicities. Arab. J. Chem. 12 (7), 908–931.Koehler, A., et al., 2024. Prediction of melanin content of Fonsecaea pedrosoi using Fourier transform infrared spectroscopy (FTIR) and chemometrics. Spectrochim. Acta Mol. Biomol. Spectrosc. 310, 123945.Kouhbanani, M.A.J., Mosleh-Shirazi, S., Beheshtkhoo, N., Kasaee, S.R., Nekouian, S., Alshehery, S., Amani, A.M., 2023. Investigation through the antimicrobial activity of electrospun PCL nanofiber mats with green synthesized Ag–Fe nanoparticles. Journal of Drug Delivery Science and Technology 85, 104541.Kumar, P., Kumar, S.V., 2023. Nanopriming of Eleusine coracana seeds using phyto- assisted magnetic nanoparticles (Fe3O4) synthesized from Colocasia esculenta leaves. Biomass Conversion and Biorefinery.Lallo da Silva, B., et al., 2019. Relationship between structure and antimicrobial activity of zinc oXide nanoparticles: an overview. Int. J. Nanomed. 14, 9395–9410.Lan, S., Lin, J., Zheng, N., 2014. Evaluation of the antioXidant activity of Coreopsis tinctoria Nuff. and optimisation of isolation by response surface methodology. Acta Pharm. 64 (3), 369–378.Lei, H., et al., 2023. Enhanced tribocatalytic degradation of organic pollutants by ZnO nanoparticles of high crystallinity. Nanomaterials 13 (1), 46.Look, D.C., 2001. Recent advances in ZnO materials and devices. Mater. Sci. Eng., B 80 (1), 383–387.Ly, N.H., Nguyen, N.B., Tran, H.N., Hoang, T.T.H., Joo, S.W., Vasseghian, Y., Klemeˇs, J. J., 2023. Metal-organic framework nanopesticide carrier for accurate pesticide delivery and decrement of groundwater pollution. J. Clean. Prod. 402, 136809.Mahdi Ismail, S.M., et al., 2023. Characterization of green synthesized of ZnO nanoparticles by using pinus brutia leaves extracts. J. Mol. Struct. 1280, 135063.Manojkumar, U., Kaliannan, D., Srinivasan, V., Balasubramanian, B., Kamyab, H., Mussa, Z.H., Palaninaicker, S., 2023. Green synthesis of zinc oXide nanoparticles using Brassica oleracea var. botrytis leaf extract: Photocatalytic, antimicrobial and larvicidal activity. Chemosphere 323, 138263.Mohammadhassani, M., Nezamabadi-Pour, H., Suhatril, M., Shariati, M., 2013. Identification of a suitable ANN architecture in predicting strain in tie section of concrete deep beams. Struct. Eng. Mech. Int. J. 46 (6), 853–868.Mohammadhassani, M., Nezamabadi-Pour, H., Suhatril, M., Shariati, M., 2014. An evolutionary fuzzy modelling approach and comparison of different methods for shear strength prediction of high-strength concrete beams without stirrups. Smart Struct. Syst. Int. J. 14 (5), 785–809.Mohd Yusof, H., et al., 2019. Microbial synthesis of zinc oXide nanoparticles and their potential application as an antimicrobial agent and a feed supplement in animal industry: a review. J. Anim. Sci. Biotechnol. 10, 1–22.Mohd Yusof, H., et al., 2020. Biosynthesis of zinc oXide nanoparticles by cell-biomass and supernatant of Lactobacillus plantarum TA4 and its antibacterial and biocompatibility properties. Sci. Rep. 10 (1), 19996.Muhammad, W., et al., 2019. Optical, morphological and biological analysis of zinc oXide nanoparticles (ZnO NPs) using. RSC Adv. 9 (51), 29541–29548.Nava, A.R., Daneshian, L., Sarma, H., 2022. Antibiotic resistant genes in the environment-exploring surveillance methods and sustainable remediation strategies of antibiotics and ARGs. Environ. Res. 215, 114212.Nikalje, A.P., 2015. Nanotechnology and its applications in medicine. Med. Chem. 5 (2), 81–89.Nilavukkarasi, M., Vijayakumar, S., Prathipkumar, S., 2020. Capparis zeylanica mediated bio-synthesized ZnO nanoparticles as antimicrobial, photocatalytic and anti-cancer applications. Materials Science for Energy Technologies 3, 335–343.Pavlova, E.L., Zografov, N.N., Simeonova, L.S., 2016. Comparative study on the antioXidant capacities of synthetic influenza inhibitors and ellagic acid in model systems. Biomed. Pharmacother. 83, 755–762.Prashanna Suvaitha, S., et al., 2023. Optical and biological properties of MgO/ZnO nanocomposite derived via eggshell membrane: a bio-waste approach. Bioproc. Biosyst. Eng. 46 (1), 39–51.Preeti, et al., 2020. ZnO quantum dots: broad spectrum microbicidal agent against multidrug resistant pathogens E. coli and C. albicans. Frontiers in Nanotechnology 2, 576342.Pushparaj, K., Liu, W.C., Meyyazhagan, A., Orlacchio, A., Pappusamy, M., Vadivalagan, C., Balasubramanian, B., 2022. Nano-from nature to nurture: A comprehensive review on facets, trends, perspectives and sustainability of nanotechnology in the food sector. Energy 240, 122732.Rad, S.S., Sani, A.M., Mohseni, S., 2019. Biosynthesis, characterization and antimicrobial activities of zinc oXide nanoparticles from leaf extract of Mentha pulegium (L.). Microb. Pathog. 131, 239–245.Rahimi, M.T., et al., 2015. Scolicidal activity of biosynthesized silver nanoparticles against Echinococcus granulosus protoscolices. Int. J. Surg. 19, 128–133.Rahman, A., et al., 2021. Zinc oXide and zinc oXide-based nanostructures: biogenic and phytogenic synthesis, properties and applications. Bioproc. Biosyst. Eng. 44 (7), 1333–1372.Raimondi, F., et al., 2005. Nanoparticles in energy technology: examples from electrochemistry and catalysis. Angew. Chem. Int. Ed. 44 (15), 2190–2209.Rajeshkumar, S., et al., 2022. Degradation of toXic dye and antimicrobial and free radical potential of environmental benign zinc oXide nanoparticles. Bioinorgan. Chem. Appl. 2022, 4513208.Rasouli, K., Rasouli, J., Mohtaram, M.S., Sabbaghi, S., Kamyab, H., Moradi, H., Chelliapan, S., 2023. Biomass-derived activated carbon nanocomposites for cleaner production: a review on aspects of photocatalytic pollutant degradation. J. Clean. Prod. 138181.Reddy, K.M., et al., 2007. Selective toXicity of zinc oXide nanoparticles to prokaryotic and eukaryotic systems. Appl. Phys. Lett. 90 (21).Regiel-Futyra, A., et al., 2015. The quenching effect of chitosan crosslinking on ZnO nanoparticles photocatalytic activity. RSC Adv. 5 (97), 80089–80097.Safa, M., Kachitvichyanukul, V., 2019. Moment rotation prediction of precast beam to column connections using extreme learning machine. Struct. Eng. Mech. Int. J. 70 (5), 639–647.Safa, M., Sari, P.A., Shariati, M., Suhatril, M., Trung, N.T., Wakil, K., Khorami, M., 2020. Development of neuro-fuzzy and neuro-bee predictive models for prediction of the safety factor of eco-protection slopes. Phys. A: Stat. Mech. Appl. 550, 124046.Safa, M., Shariati, M., Ibrahim, Z., Toghroli, A., Baharom, S.B., Nor, N.M., Petkovi´c, D., 2016. Potential of adaptive neuro fuzzy inference system for evaluating the factors affecting steel-concrete composite beam’s shear strength. Steel Compos. Struct. Int. J. 21 (3), 679–688.Salem, W., et al., 2015. Antibacterial activity of silver and zinc nanoparticles against Vibrio cholerae and enterotoXic Escherichia coli. International Journal of Medical Microbiology 305 (1), 85–95.Santhoshkumar, J., Kumar, S.V., Rajeshkumar, S., 2017. Synthesis of zinc oXide nanoparticles using plant leaf extract against urinary tract infection pathogen. Resource-Efficient Technologies 3 (4), 459–465.Sˇebesta, M., et al., 2019. Increased colloidal stability and decreased solubility—sol—gel synthesis of zinc oXide nanoparticles with humic acids. J. Nanosci. Nanotechnol. 19 (5), 3024–3030.Sedghi, Y., Zandi, Y., Shariati, M., Ahmadi, E., Azar, V.M., Toghroli, A., Wakil, K., 2018. Application of ANFIS technique on performance of C and L shaped angle shear connectors. Smart Struc. Systems 22 (3), 335–340.Segets, D., et al., 2009. Analysis of optical absorbance spectra for the determination of ZnO nanoparticle size distribution, solubility, and surface energy. ACS Nano 3 (7), 1703–1710.Senguttuvan, J., Paulsamy, S., Karthika, K., 2014. Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. for in vitro antioXidant activities. Asian Pac. J. Trop. Biomed. 4, S359–S367.Senthilkumar, R., et al., 2018. Phytochemical screening of aqueous leaf extract of Sida acuta burm. F. And its antibacterial activity. Journal of Emerging Technologies and Innovative Research 5 (8), 474–478.Seshadri, V.D., 2021. Zinc oXide nanoparticles from Cassia auriculata flowers showed the potent antimicrobial and in vitro anticancer activity against the osteosarcoma MG- 63 cells. Saudi J. Biol. Sci. 28 (7), 4046–4054.Shariati, M., Mafipour, M.S., Haido, J.H., Yousif, S.T., Toghroli, A., Trung, N.T., Shariati, A., 2020a. Identification of the most influencing parameters on the properties of corroded concrete beams using an Adaptive Neuro-Fuzzy Inference System (ANFIS). Steel Compos. Struct. 34 (1), 155.Shariati, M., Mafipour, M.S., Mehrabi, P., Ahmadi, M., Wakil, K., Trung, N.T., Toghroli, A., 2020b. Prediction of concrete strength in presence of furnace slag and fly ash using Hybrid ANN-GA (Artificial Neural Network-Genetic Algorithm). Smart Structu. Systems Int. J. 25 (2), 183–195.Shariati, M., Mafipour, M.S., Ghahremani, B., Azarhomayun, F., Ahmadi, M., Trung, N. T., Shariati, A., 2022. A novel hybrid extreme learning machine–grey wolf optimizer (ELM-GWO) model to predict compressive strength of concrete with partial replacements for cement. Eng. Comput. 1–23.Shariati, M., Mafipour, M.S., Mehrabi, P., Bahadori, A., Zandi, Y., Salih, M.N., Poi- Ngian, S., 2019. Application of a hybrid artificial neural network-particle swarm optimization (ANN-PSO) model in behavior prediction of channel shear connectors embedded in normal and high-strength concrete. Applied Sci. 9 (24), 5534.Shittu, M., Alagbe, J., 2020. Phyto-nutritional profiles of broom weed (Sida acuta) leaf extract. International Journal on Integrated Education 3 (11), 119–124.Siddheswaran, R., et al., 2013. Fabrication and characterization of a diluted magnetic semiconducting TM co-doped Al: ZnO (TM Co, Ni) thin films by sol–gel spin coating method. Spectrochim. Acta Mol. Biomol. Spectrosc. 106, 118–123.Sidebottom, D.L., 2024. Dynamic light scattering study of the non-exponential α-relaxation in sodium germanate glass melts. J. Non-Cryst. Solids 627, 122819.Silva, G.A., 2004. Introduction to nanotechnology and its applications to medicine. Surg. Neurol. 61 (3), 216–220.Singh, J., et al., 2018. ‘Green’synthesis of metals and their oXide nanoparticles: applications for environmental remediation. J. Nanobiotechnol. 16 (1), 1–24.Singh, A., et al., 2020. Green synthesis of metallic nanoparticles as effective alternatives to treat antibiotics resistant bacterial infections: a review. Biotechnology Reports 25, e00427.Sirelkhatim, A., et al., 2015. Review on zinc oxide nanoparticles: antibacterial activity and toxicity mechanism. Nano-Micro Lett. 7, 219–242.Srivastava, V., Gusain, D., Sharma, Y.C., 2013. Synthesis, characterization and application of zinc oxide nanoparticles (n-ZnO). Ceram. Int. 39 (8), 9803–9808.Stoimenov, P.K., et al., 2002. Metal oxide nanoparticles as bactericidal agents. Langmuir 18 (17), 6679–6686.Sultanova, N., et al., 2001. Antioxidant and antimicrobial activities of Tamarix ramosissima. J. Ethnopharmacol. 78 (2), 201–205.Syama, S., et al., 2014. Zinc oxide nanoparticles induced oxidative stress in mouse bone marrow mesenchymal stem cells. Toxicol. Mech. Methods 24 (9), 644–653.Tan, Q., et al., 2019. Hierarchical zinc oxide/reduced graphene oxide composite: preparation route, mechanism study and lithium ion storage. J. Colloid Interface Sci. 548, 233–243.Tavakkoli, O., Kamyab, H., Shariati, M., Mohamed, A.M., Junin, R., 2022. Effect of nanoparticles on the performance of polymer/surfactant flooding for enhanced oil recovery: A review. Fuel 312, 122867.Titus, D., James Jebaseelan Samuel, E., Roopan, S.M., 2019. Chapter 12 - nanoparticle characterization techniques. In: Shukla, A.K., Iravani, S. (Eds.), Green Synthesis, Characterization and Applications of Nanoparticles. Elsevier, pp. 303–319.Toghroli, A., Mohammadhassani, M., Suhatril, M., Shariati, M., Ibrahim, Z., 2014. Prediction of shear capacity of channel shear connectors using the ANFIS model. Steel Compos. Struct. 17 (5), 623–639.Veerakumar, K., Govindarajan, M., Rajeswary, M., 2013. Green synthesis of silver nanoparticles using Sida acuta (Malvaceae) leaf extract against Culex quinquefasciatus, Anopheles stephensi, and Aedes aegypti (Diptera: Culicidae). Parasitol. Res. 112 (12), 4073–4085.Verma, C., et al., 2018. An overview on plant extracts as environmental sustainable and green corrosion inhibitors for metals and alloys in aggressive corrosive media. J. Mol. Liq. 266, 577–590.Win, T.T., et al., 2021. Green synthesis and characterization of Fe3O4 nanoparticles using Chlorella-K01 extract for potential enhancement of plant growth stimulating and antifungal activity. Sci. Rep. 11 (1), 21996.Xia, C., et al., 2023. Optimistic and possible contribution of nanomaterial on biomedical applications: a review. Environ. Res. 218, 114921.Xia, C., Ren, T., Darabi, R., Shabani-Nooshabadi, M., Klemeˇs, J.J., Karaman, C., Chelliapan, S., 2023. Spotlighting the boosted energy storage capacity of CoFe2O4/ Graphene nanoribbons: A promising positive electrode material for high-energydensity asymmetric supercapacitor. Energy 270, 126914.Yazdani, M., Kabirifar, K., Frimpong, B.E., Shariati, M., Mirmozaffari, M., Boskabadi, A., 2021. Improving construction and demolition waste collection service in an urban area using a simheuristic approach: A case study in Sydney, Australia. J. Clean. Prod. 280, 124138.Zainah, T.A.S.M.I., Shahaboddin, S.M.S.M.S., 1801. Potential of soft computing approach for evaluating the factors affecting the capacity of steel–concrete composite beam. J. Intell. Manuf. 29 (8), 1793.Zhang, Z., Karimi-Maleh, H., 2023. In situ synthesis of label-free electrochemical aptasensor-based sandwich-like AuNPs/PPy/Ti3C2Tx for ultrasensitive detection of lead ions as hazardous pollutants in environmental fluids. Chemosphere 324, 138302.Zhu, X., Pathakoti, K., Hwang, H.-M., 2019. Chapter 10 - green synthesis of titanium dioxide and zinc oxide nanoparticles and their usage for antimicrobial applications and environmental remediation. In: Shukla, A.K., Iravani, S. (Eds.), Green Synthesis, Characterization and Applications of Nanoparticles. Elsevier, pp. 223–263.281258Zinc oxide NPs synthesisSida acutaAntibacterial efficacyAntioxidant potentialAntibacterial activityCatalytic dye degradationConvolutional neural network (CNN)PublicationORIGINALGreen synthesis of zinc oxide nanoparticles from Sida acuta leaf extract for.pdfGreen synthesis of zinc oxide nanoparticles from Sida acuta leaf extract for.pdfapplication/pdf13786914https://repositorio.cuc.edu.co/bitstreams/21991fb6-89d4-407a-9b18-7915de89d4f5/downloade705ec73a9ca06b0624da42bfc0d8a52MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-815543https://repositorio.cuc.edu.co/bitstreams/8102ef1c-bcaa-4703-8a60-5f35506ec087/download73a5432e0b76442b22b026844140d683MD52TEXTGreen synthesis of zinc oxide nanoparticles from Sida acuta leaf extract for.pdf.txtGreen synthesis of zinc oxide nanoparticles from Sida acuta leaf extract for.pdf.txtExtracted texttext/plain100413https://repositorio.cuc.edu.co/bitstreams/a464a150-1976-4d7b-95a8-fa0a01a1fa06/download4f643f6f8809401c9154974f294721eeMD53THUMBNAILGreen synthesis of zinc oxide nanoparticles from Sida acuta leaf extract for.pdf.jpgGreen synthesis of zinc oxide nanoparticles from Sida acuta leaf extract for.pdf.jpgGenerated Thumbnailimage/jpeg13941https://repositorio.cuc.edu.co/bitstreams/a0bb4c4a-2124-4fdd-bd9c-8d981ef75f56/download3aceb2e9a350cc9015cce94c3464eefeMD5411323/13667oai:repositorio.cuc.edu.co:11323/136672024-11-13 03:00:50.829https://creativecommons.org/licenses/by-nc-nd/4.0/© 2024 Published by Elsevier Inc.open.accesshttps://repositorio.cuc.edu.coRepositorio de la Universidad de la Costa 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ara ejercer estos derechos sobre la Obra tal y como se indica a continuación:</p>
    <ol type="a">
      <li>Reproducir la Obra, incorporar la Obra en una o más Obras Colectivas, y reproducir la Obra incorporada en las Obras Colectivas.</li>
      <li>Distribuir copias o fonogramas de las Obras, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública, incluyéndolas como incorporadas en Obras Colectivas, según corresponda.</li>
      <li>Distribuir copias de las Obras Derivadas que se generen, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública.</li>
    </ol>
    <p>Los derechos mencionados anteriormente pueden ser ejercidos en todos los medios y formatos, actualmente conocidos o que se inventen en el futuro. Los derechos antes mencionados incluyen el derecho a realizar dichas modificaciones en la medida que sean técnicamente necesarias para ejercer los derechos en otro medio o formatos, pero de otra manera usted no está autorizado para realizar obras derivadas. Todos los derechos no otorgados expresamente por el Licenciante quedan por este medio reservados, incluyendo pero sin limitarse a aquellos que se mencionan en las secciones 4(d) y 4(e).</p>
  </li>
  <br/>
  <li>
    Restricciones.
    <p>La licencia otorgada en la anterior Sección 3 está expresamente sujeta y limitada por las siguientes restricciones:</p>
    <ol type="a">
      <li>Usted puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra sólo bajo las condiciones de esta Licencia, y Usted debe incluir una copia de esta licencia o del Identificador Universal de Recursos de la misma con cada copia de la Obra que distribuya, exhiba públicamente, ejecute públicamente o ponga a disposición pública. No es posible ofrecer o imponer ninguna condición sobre la Obra que altere o limite las condiciones de esta Licencia o el ejercicio de los derechos de los destinatarios otorgados en este documento. No es posible sublicenciar la Obra. Usted debe mantener intactos todos los avisos que hagan referencia a esta Licencia y a la cláusula de limitación de garantías. Usted no puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra con alguna medida tecnológica que controle el acceso o la utilización de ella de una forma que sea inconsistente con las condiciones de esta Licencia. Lo anterior se aplica a la Obra incorporada a una Obra Colectiva, pero esto no exige que la Obra Colectiva aparte de la obra misma quede sujeta a las condiciones de esta Licencia. Si Usted crea una Obra Colectiva, previo aviso de cualquier Licenciante debe, en la medida de lo posible, eliminar de la Obra Colectiva cualquier referencia a dicho Licenciante o al Autor Original, según lo solicitado por el Licenciante y conforme lo exige la cláusula 4(c).</li>
      <li>Usted no puede ejercer ninguno de los derechos que le han sido otorgados en la Sección 3 precedente de modo que estén principalmente destinados o directamente dirigidos a conseguir un provecho comercial o una compensación monetaria privada. El intercambio de la Obra por otras obras protegidas por derechos de autor, ya sea a través de un sistema para compartir archivos digitales (digital file-sharing) o de cualquier otra manera no será considerado como estar destinado principalmente o dirigido directamente a conseguir un provecho comercial o una compensación monetaria privada, siempre que no se realice un pago mediante una compensación monetaria en relación con el intercambio de obras protegidas por el derecho de autor.</li>
      <li>Si usted distribuye, exhibe públicamente, ejecuta públicamente o ejecuta públicamente en forma digital la Obra o cualquier Obra Derivada u Obra Colectiva, Usted debe mantener intacta toda la información de derecho de autor de la Obra y proporcionar, de forma razonable según el medio o manera que Usted esté utilizando: (i) el nombre del Autor Original si está provisto (o seudónimo, si fuere aplicable), y/o (ii) el nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.</li>
      <li>
        Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:
        <ol type="i">
          <li>Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.</li>
          <li>Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, los consagrados por la SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.</li>
        </ol>
      </li>
      <li>Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, ACINPRO), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.</li>
    </ol>
  </li>
  <br/>
  <li>
    Representaciones, Garantías y Limitaciones de Responsabilidad.
    <p>A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.</p>
  </li>
  <br/>
  <li>
    Limitación de responsabilidad.
    <p>A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.</p>
  </li>
  <br/>
  <li>
    Término.
    <ol type="a">
      <li>Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.</li>
      <li>Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.</li>
    </ol>
  </li>
  <br/>
  <li>
    Varios.
    <ol type="a">
      <li>Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.</li>
      <li>Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.</li>
      <li>Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.</li>
      <li>Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.</li>
    </ol>
  </li>
  <br/>
</ol>
 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