P07-22 Particulate matter of Barranquilla (Colombia) affects reproduction and survival of Caenorhabditis elegans

Particulate matter is one of the main environmental issues in large cities due to traffic and industrial activity. Barranquilla is a city with more than 2 million habitants city with an intense port and industrial economy. In this work, we collected PM2.5 samples from the industrial area of Barranquilla between July and September 2021, and obtained aqueous extracts from these samples. The nematode Caenorhabditis elegans was the toxicological model in this research. The wild type strain N2 was used for survival and reproduction assays. Previously, worms were subjected to age-synchronization via bleach solution. Experiments were performed using whole extract and dilutions of 50% and 33% to analyze dose dependence. For survival, about 10 nematodes in larval age L4 were exposed for 24 h to whole extract and dilutions. Four replicates for treatment were made and each experiment was conducted three. For reproduction, ten nematodes in larval stage L4 were exposed for 24 h to whole extract and dilutions. Each nematode was set individually in K agar-plates seeded with E. coli OP50, and the number of offspring at all stages was counted after 72 h. The results showed that the dust extracts to which the nematodes were exposed did not cause mortality in any dilution made, or with the complete extract. However, the extracts had sublethal effects that affected the reproduction of the model, by decreasing the number of eggs laid after 72 h of exposure to the dust extracts. This effect was concentration-dependent but did not vary with the month of collection, probably due to climate stability at this time of year in the Caribbean. This study is the first approximation of the toxicological and environmental behaviour of PM2.5 in the North of Colombia and verifies the usefulness of Caenorhabditis elegans as a biological model.

• Autores: Arroyo, B.J.; Tejeda-Benitez, L.P.; Sierra-Marquez, L.I.; Moreno-Rios, A.L.; Godin-Arroyo, B.S.; Martinez-Avila, M.C.; Rodriguez Beltran, S.
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2022
• Institución: Corporación Universidad de la Costa
• Repositorio: REDICUC - Repositorio CUC
• Idioma: eng
• OAI Identifier: oai:repositorio.cuc.edu.co:11323/10799
• Acceso en línea: https://hdl.handle.net/11323/10799; https://repositorio.cuc.edu.co/
• Palabra clave: Caenorhabditis elegans; Reproduction; Survival
• Rights: embargoedAccess
• License: Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)