Evolutionary echoes: A four-day fasting and low-caloric intake study on autonomic modulation and physiological adaptations in humans

This study evaluates the psychophysiological response to a simulated hunter–gatherer endurance task with restricted caloric intake over four days. It assesses changes in body composition, autonomic modulation, and physical and cognitive performance. Participants underwent daily 8 h fasted walks foll...

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Autores:
Belinchón-deMiguel, Pedro
Navarro-Jiménez, Eduardo
Laborde-Cárdenas, Carmen Cecilia
Clemente-Suárez, Vicente Javier
Tipo de recurso:
Article of investigation
Fecha de publicación:
2024
Institución:
Corporación Universidad de la Costa
Repositorio:
REDICUC - Repositorio CUC
Idioma:
eng
OAI Identifier:
oai:repositorio.cuc.edu.co:11323/13502
Acceso en línea:
https://hdl.handle.net/11323/13502
https://repositorio.cuc.edu.co/
Palabra clave:
Fasting
Ultraendurance
Autonomic modulation
Heart rate variability
Hunter–gatherer diet
Body composition
Rights
openAccess
License
Atribución 4.0 Internacional (CC BY 4.0)
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oai_identifier_str oai:repositorio.cuc.edu.co:11323/13502
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network_name_str REDICUC - Repositorio CUC
repository_id_str
dc.title.eng.fl_str_mv Evolutionary echoes: A four-day fasting and low-caloric intake study on autonomic modulation and physiological adaptations in humans
title Evolutionary echoes: A four-day fasting and low-caloric intake study on autonomic modulation and physiological adaptations in humans
spellingShingle Evolutionary echoes: A four-day fasting and low-caloric intake study on autonomic modulation and physiological adaptations in humans
Fasting
Ultraendurance
Autonomic modulation
Heart rate variability
Hunter–gatherer diet
Body composition
title_short Evolutionary echoes: A four-day fasting and low-caloric intake study on autonomic modulation and physiological adaptations in humans
title_full Evolutionary echoes: A four-day fasting and low-caloric intake study on autonomic modulation and physiological adaptations in humans
title_fullStr Evolutionary echoes: A four-day fasting and low-caloric intake study on autonomic modulation and physiological adaptations in humans
title_full_unstemmed Evolutionary echoes: A four-day fasting and low-caloric intake study on autonomic modulation and physiological adaptations in humans
title_sort Evolutionary echoes: A four-day fasting and low-caloric intake study on autonomic modulation and physiological adaptations in humans
dc.creator.fl_str_mv Belinchón-deMiguel, Pedro
Navarro-Jiménez, Eduardo
Laborde-Cárdenas, Carmen Cecilia
Clemente-Suárez, Vicente Javier
dc.contributor.author.none.fl_str_mv Belinchón-deMiguel, Pedro
Navarro-Jiménez, Eduardo
Laborde-Cárdenas, Carmen Cecilia
Clemente-Suárez, Vicente Javier
dc.subject.proposal.eng.fl_str_mv Fasting
Ultraendurance
Autonomic modulation
Heart rate variability
Hunter–gatherer diet
Body composition
topic Fasting
Ultraendurance
Autonomic modulation
Heart rate variability
Hunter–gatherer diet
Body composition
description This study evaluates the psychophysiological response to a simulated hunter–gatherer endurance task with restricted caloric intake over four days. It assesses changes in body composition, autonomic modulation, and physical and cognitive performance. Participants underwent daily 8 h fasted walks followed by a 150 kcal meal to replicate hunter–gatherer activity and dietary patterns. Measurements of metabolic, respiratory, and subjective well-being, along with heart rate variability (HRV) monitoring, were conducted pre- and post-activity to evaluate the impact of endurance activity under caloric restriction. We found weight loss, decreased body and visceral fat, and reduced skeletal muscle mass and water percentage. High sympathetic activation and stable urinary markers, except for increased proteinuria, indicated stress responses and muscular degradation. Elevated perceived exertion post-exercise with good adaptation to prolonged effort underlines the body’s adaptability to ancestral lifestyle conditions, highlighting the connection among endurance, nutrition, and psychophysiological health.
publishDate 2024
dc.date.accessioned.none.fl_str_mv 2024-10-23T16:33:33Z
dc.date.available.none.fl_str_mv 2024-10-23T16:33:33Z
dc.date.issued.none.fl_str_mv 2024-03-29
dc.type.none.fl_str_mv Artículo de revista
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.content.none.fl_str_mv Text
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
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dc.type.version.none.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.citation.none.fl_str_mv Belinchón-deMiguel, P.; Navarro-Jiménez, E.; LabordeCárdenas, C.C.; Clemente-Suárez, V.J. Evolutionary Echoes: A Four-Day Fasting and Low-Caloric Intake Study on Autonomic Modulation and Physiological Adaptations in Humans. Life 2024, 14, 456. https://doi.org/ 10.3390/life14040456
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/11323/13502
dc.identifier.doi.none.fl_str_mv 10.3390/life14040456
dc.identifier.eissn.none.fl_str_mv 2075-1729
dc.identifier.instname.none.fl_str_mv Corporación Universidad de la Costa
dc.identifier.reponame.none.fl_str_mv REDICUC - Repositorio CUC
dc.identifier.repourl.none.fl_str_mv https://repositorio.cuc.edu.co/
identifier_str_mv Belinchón-deMiguel, P.; Navarro-Jiménez, E.; LabordeCárdenas, C.C.; Clemente-Suárez, V.J. Evolutionary Echoes: A Four-Day Fasting and Low-Caloric Intake Study on Autonomic Modulation and Physiological Adaptations in Humans. Life 2024, 14, 456. https://doi.org/ 10.3390/life14040456
10.3390/life14040456
2075-1729
Corporación Universidad de la Costa
REDICUC - Repositorio CUC
url https://hdl.handle.net/11323/13502
https://repositorio.cuc.edu.co/
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartofjournal.none.fl_str_mv Life
dc.relation.references.none.fl_str_mv 1. Di Liegro, C.M.; Schiera, G.; Proia, P.; Di Liegro, I. Physical Activity and Brain Health. Genes 2019, 10, 720. [CrossRef]
2. O’Keefe, J.H.; Vogel, R.; Lavie, C.J.; Cordain, L. Exercise Like a Hunter-Gatherer: A Prescription for Organic Physical Fitness. Prog. Cardiovasc. Dis. 2011, 53, 471–479. [CrossRef]
3. Wheeler, P. The evolution of bipedality and loss of functional body hair in hominids. J. Hum. Evol. 1984, 13, 91–98. [CrossRef]
4. Bramble, D.M.; Lieberman, D.E. Endurance running and the evolution of Homo. Nature 2004, 432, 345–352. [CrossRef]
5. Warburton, D.E.R.; Nicol, C.W.; Bredin, S.S.D. Health benefits of physical activity: The evidence. Can. Med. Assoc. J. 2006, 174, 801–809. [CrossRef]
6. Clemente-Suárez, V.J. Multidisciplinary intervention in the treatment of mixed anxiety and depression disorder. Physiol. Behav. 2020, 219, 112858. [CrossRef] [PubMed]
7. Harris, C.D.; Watson, K.B.; Carlson, S.A.; Fulton, J.E.; Dorn, J.M.; Elam-Evans, L. Adult Participation in Aerobic and MuscleStrengthening Physical Activities—United States, 2011. Morb. Mortal. Wkly. Rep. 2013, 62, 326–330.
8. Sponheimer, M.; Alemseged, Z.; Cerling, T.E.; Grine, F.E.; Kimbel, W.H.; Leakey, M.G.; Lee-Thorp, J.A.; Manthi, F.K.; Reed, K.E.; Wood, B.A.; et al. Isotopic evidence of early hominin diets. Proc. Natl. Acad. Sci. USA 2013, 110, 10513–10518. [CrossRef]
9. Ungar, P.S.; Sponheimer, M. The Diets of Early Hominins. Science 2011, 334, 190–193. [CrossRef] [PubMed]
10. Thompson, J.C.; Carvalho, S.; Marean, C.W.; Alemseged, Z. Origins of the Human Predatory Pattern: The Transition to LargeAnimal Exploitation by Early Hominins. Curr. Anthr. 2019, 60, 1–23. [CrossRef]
11. Fontana, L.; Partridge, L. Promoting Health and Longevity through Diet: From Model Organisms to Humans. Cell 2015, 161, 106–118. [CrossRef] [PubMed]
12. McMorris, T.; Sproule, J.; Turner, A.; Hale, B.J. Acute, intermediate intensity exercise, and speed and accuracy in working memory tasks: A meta-analytical comparison of effects. Physiol. Behav. 2011, 102, 421–428. [CrossRef]
13. Dishman, R.K.; Berthoud, H.; Booth, F.W.; Cotman, C.W.; Edgerton, V.R.; Fleshner, M.R.; Gandevia, S.C.; Gomez-Pinilla, F.; Greenwood, B.N.; Hillman, C.H.; et al. Neurobiology of Exercise. Obesity 2006, 14, 345–356. [CrossRef] [PubMed]
14. Messina, A.; Monda, M.; Valenzano, A.; Messina, G.; Villano, I.; Moscatelli, F.; Cibelli, G.; Marsala, G.; Polito, R.; Ruberto, M.; et al. Functional Changes Induced by Orexin A and Adiponectin on the Sympathetic/Parasympathetic Balance. Front. Physiol. 2018, 9, 259. [CrossRef] [PubMed]
15. Polito, R.; Valenzano, A.; Scarinci, A.; Villano, I.; Monda, M.; Messina, A.; Cibelli, G.; Porro, C.; La Torre, E.; Pisanelli, D.; et al. Very Low-Calorie Ketogenic Diet Modulates the Autonomic Nervous System Activity through Salivary Amylase in Obese Population Subjects. Int. J. Environ. Res. Public Health 2021, 18, 8475. [CrossRef] [PubMed]
16. Mammen, G.; Faulkner, G. Physical activity and the prevention of depression: A systematic review of prospective studies. Am. J. Prev. Med. 2013, 45, 649–657. [CrossRef]
17. Erickson, K.I.; Hillman, C.; Stillman, C.M.; Ballard, R.M.; Bloodgood, B.; Conroy, D.E.; Macko, R.; Marquez, D.X.; Petruzzello, S.J.; Powell, K.E.; et al. Physical Activity, Cognition, and Brain Outcomes: A Review of the 2018 Physical Activity Guidelines. Med. Sci. Sports Exerc. 2019, 51, 1242–1251. [CrossRef]
18. Clael, S.; Campos, L.F.; Correia, K.L.; de Lucena, J.M.S.; Gentil, P.; Durigan, J.L.; Ribeiro, A.L.d.A.; Martins, W.R. Exercise interventions can improve muscle strength, endurance, and electrical activity of lumbar extensors in individuals with non-specific low back pain: A systematic review with meta-analysis. Sci. Rep. 2021, 11, 16842. [CrossRef]
19. Belinchón-Demiguel, P.; Ramos-Campo, D.J.; Clemente-Suárez, V.J. Exploring the Evolutionary Disparities: A Case Study on the Psychophysiological Response to Recreating the Hunter–Gatherer Lifestyle through Physical Activity and Caloric Restriction. Appl. Sci. 2023, 13, 11140. [CrossRef]
20. Calbet, J.A.L.; Ponce-González, J.G.; Pérez-Suárez, I.; Herrero, J.d.l.C.; Holmberg, H. A time-efficient reduction of fat mass in 4 days with exercise and caloric restriction. Scand. J. Med. Sci. Sports 2015, 25, 223–233. [CrossRef] [PubMed]
21. Arakawa, K.; Hosono, A.; Shibata, K.; Ghadimi, R.; Fuku, M.; Goto, C.; Imaeda, N.; Tokudome, Y.; Hoshino, H.; Marumoto, M.; et al. Changes in blood biochemical markers before, during, and after a 2-day ultramarathon. Open Access J. Sports Med. 2016, 7, 43–50. [CrossRef]
22. Korobeynikov, G.; Korobeynikova, L.; Iermakov, S.; Nosko, M. Reaction of Heart Rate Regulation to Extreme Sport Activity in Elite Athletes. 2016. Available online: http://erpub.chnpu.edu.ua:8080/jspui/bitstream/123456789/194/1/Reaction%20of%20heart% 20rate%20regulation%20to%20extreme%20sport%20activity%20in%20elite%20athletes.pdf (accessed on 27 November 2023).
23. Kajaia, T.; Maskhulia, L.; Chelidze, K.; Akhalkatsi, V.; Kakhabrishvili, Z. The effects of non-functional overreaching and overtraining on autonomic nervous system function in highly trained Georgian athletes. Georgian Med. Newa 2017, 3, 97–101.
24. Belinchón-Demiguel, P.; Tornero-Aguilera, J.F.; Dalamitros, A.A.; Nikolaidis, P.T.; Rosemann, T.; Knechtle, B.; Clemente-Suárez, V.J. Multidisciplinary Analysis of Differences Between Finisher and Non-finisher Ultra-Endurance Mountain Athletes. Front. Physiol. 2019, 10, 1507. [CrossRef]
25. Belinchon-Demiguel, P.; Clemente-Suárez, V.J. Psychophysiological, Body Composition, Biomechanical and Autonomic Modulation Analysis Procedures in an Ultraendurance Mountain Race. J. Med. Syst. 2018, 42, 32. [CrossRef] [PubMed]
26. Garcia, J.R.; Manimaleth, R.; Czartoryski, P.; Napolitano, P.; Watters, H.; Weber, C.; Alvarez-Beaton, A.; Nieto, A.C.; Patel, A.; Peacock, C. A Comparative Study of Body Composition Assessment Techniques: DXA and InBody 270. J. Exerc. Nutr. 2020, 3, 3.
27. Tornero-Aguilera, J.F.; Clemente-Suárez, V.J. Cognitive and psychophysiological impact of surgical mask use during university lessons. Physiol. Behav. 2021, 234, 113342. [CrossRef] [PubMed]
28. Borg, G. Perceived exertion as an indicator of somatic stress. J. Rehabil. Med. 1970, 2, 92–98. [CrossRef]
29. Willis, E.A.; Creasy, S.A.; Honas, J.J.; Melanson, E.L.; Donnelly, J.E. The effects of exercise session timing on weight loss and components of energy balance: Midwest exercise trial 2. Int. J. Obes. 2020, 44, 114–124. [CrossRef]
30. Swift, D.L.; Johannsen, N.M.; Lavie, C.J.; Earnest, C.P.; Church, T.S. The Role of Exercise and Physical Activity in Weight Loss and Maintenance. Prog. Cardiovasc. Dis. 2014, 56, 441–447. [CrossRef]
31. Cahill, G.F., Jr. Fuel Metabolism in Starvation. Annu. Rev. Nutr. 2006, 26, 1–22. [CrossRef]
32. Phinney, S.; Bistrian, B.; Evans, W.; Gervino, E.; Blackburn, G. The human metabolic response to chronic ketosis without caloric restriction: Preservation of submaximal exercise capability with reduced carbohydrate oxidation. Metabolism 1983, 32, 769–776. [CrossRef]
33. Volek, J.S.; Freidenreich, D.J.; Saenz, C.; Kunces, L.J.; Creighton, B.C.; Bartley, J.M.; Davitt, P.M.; Munoz, C.X.; Anderson, J.M.; Maresh, C.M.; et al. Metabolic characteristics of keto-adapted ultra-endurance runners. Metabolism 2016, 65, 100–110. [CrossRef]
34. Most, J.; Redman, L.M. Impact of calorie restriction on energy metabolism in humans. Exp. Gerontol. 2020, 133, 110875. [CrossRef]
35. Neeland, I.J.; Ross, R.; Després, J.-P.; Matsuzawa, Y.; Yamashita, S.; Shai, I.; Seidell, J.; Magni, P.; Santos, R.D.; Arsenault, B.; et al. Visceral and ectopic fat, atherosclerosis, and cardiometabolic disease: A position statement. Lancet Diabetes Endocrinol. 2019, 7, 715–725. [CrossRef]
36. Ismail, I.; Keating, S.E.; Baker, M.K.; Johnson, N.A. A systematic review and meta-analysis of the effect of aerobic vs. resistance exercise training on visceral fat. Obes. Rev. 2012, 13, 68–91. [CrossRef]
37. Posadzki, P.; Pieper, D.; Bajpai, R.; Makaruk, H.; Könsgen, N.; Neuhaus, A.L.; Semwal, M. Exercise/physical activity and health outcomes: An overview of Cochrane systematic reviews. BMC Public Health 2020, 20, 1724. [CrossRef]
38. You, T.; Murphy, K.M.; Lyles, M.F.; Demons, J.L.; Lenchik, L.; Nicklas, B.J. Addition of aerobic exercise to dietary weight loss preferentially reduces abdominal adipocyte size. Int. J. Obes. 2006, 30, 1211–1216. [CrossRef]
39. Basile, A.J.; Renner, M.W.; Hidaka, B.H.; Sweazea, K.L. An evolutionary mismatch narrative to improve lifestyle medicine: A patient education hypothesis. Evol. Med. Public Health 2021, 9, 157–163. [CrossRef]
40. Tiller, N.B.; Chiesa, S.T.; Roberts, J.D.; Turner, L.A.; Jones, S.; Romer, L.M. Physiological and Pathophysiological Consequences of a 25-Day Ultra-Endurance Exercise Challenge. Front. Physiol. 2019, 10, 589. [CrossRef] [PubMed]
41. Heydenreich, J.; Kayser, B.; Schutz, Y.; Melzer, K. Total Energy Expenditure, Energy Intake, and Body Composition in Endurance Athletes Across the Training Season: A Systematic Review. Sports Med. Open 2017, 3, 8. [CrossRef] [PubMed]
42. Wang, X.H.; Mitch, W.E.; Price, S.R. Pathophysiological mechanisms leading to muscle loss in chronic kidney disease. Nat. Rev. Nephrol. 2021, 18, 138–152. [CrossRef]
43. Kinrade, E.J.; Galloway, S.D.R. Dietary Observations of Ultra-Endurance Runners in Preparation for and During a Continuous 24-h Event. Front. Physiol. 2021, 12. [CrossRef]
44. Kreider, R.B.; Wilborn, C.D.; Taylor, L.; Campbell, B.; Almada, A.L.; Collins, R.; Cooke, M.; Earnest, C.P.; Greenwood, M.; Kalman, D.S.; et al. ISSN exercise & sport nutrition review: Research & recommendations. J. Int. Soc. Sports Nutr. 2010, 7, 7. [CrossRef]
45. Hoffman, M.D. Proper Hydration During Ultra-endurance Activities. Sports Med. Arthrosc. Rev. 2019, 27, 8–14. [CrossRef]
46. Vicente-Salar, N.; Otegui, A.U.; Collado, E.R. Endurance Training in Fasting Conditions: Biological Adaptations and Body Weight Management. Nutr. Hosp. 2015, 32, 2409–2420
47. Clemente-Suárez, V.J. Psychophysiological response and energy balance during a 14-h ultraendurance mountain running event. Appl. Physiol. Nutr. Metab. 2015, 40, 269–273. [CrossRef]
48. Moore, R.D.; Romine, M.W.; O’Connor, P.J.; Tomporowski, P.D. The influence of exercise-induced fatigue on cognitive function. J. Sports Sci. 2012, 30, 841–850. [CrossRef]
49. Wollseiffen, P.; Schneider, S.; Martin, L.A.; Kerhervé, H.A.; Klein, T.; Solomon, C. The effect of 6 h of running on brain activity, mood, and cognitive performance. Exp. Brain Res. 2016, 234, 1829–1836. [CrossRef] [PubMed]
50. Pontzer, H. Economy and Endurance in Human Evolution. Curr. Biol. 2017, 27, R613–R621. [CrossRef] [PubMed]
51. Pettersen, S.D.; Aslaksen, P.M.; Pettersen, S.A. Pain processing in elite and high-level athletes compared to non-athletes. Front. Psychol. 2020, 11, 1908. [CrossRef] [PubMed]
52. Hoffman, M.D.; Fogard, K. Factors Related to Successful Completion of a 161-km Ultramarathon. Int. J. Sports Physiol. Perform. 2011, 6, 25–37. [CrossRef]
53. Berger, N.J.A.; Best, R.; Best, A.W.; Lane, A.M.; Millet, G.Y.; Barwood, M.; Marcora, S.; Wilson, P.; Bearden, S. Limits of Ultra: Towards an Interdisciplinary Understanding of Ultra-Endurance Running Performance. Sports Med. 2023, 54, 73–93. [CrossRef] [PubMed]
54. Best, R.; Barwick, B.; Best, A.; Berger, N.; Harrison, C.; Wright, M.; Sparrow, J. Changes in Pain and Nutritional Intake Modulate Ultra-Running Performance: A Case Report. Sports 2018, 6, 111. [CrossRef] [PubMed]
55. Herbert, B.M.; Pollatos, O. Attenuated interoceptive sensitivity in overweight and obese individuals. Eat. Behav. 2014, 15, 445–448. [CrossRef] [PubMed]
56. Martínez-Navarro, I.; Sanchez-Gómez, J.M.; Aparicio, I.; Priego-Quesada, J.I.; Pérez-Soriano, P.; Collado, E.; Hernando, B.; Hernando, C. Effect of mountain ultramarathon distance competition on biochemical variables, respiratory and lower-limb fatigue. PLoS ONE 2020, 15, e0238846. [CrossRef]
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rights_invalid_str_mv Atribución 4.0 Internacional (CC BY 4.0)
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spelling Atribución 4.0 Internacional (CC BY 4.0)© 2024 by the authors. Licensee MDPI, Basel, Switzerlandhttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Belinchón-deMiguel, PedroNavarro-Jiménez, EduardoLaborde-Cárdenas, Carmen CeciliaClemente-Suárez, Vicente Javier2024-10-23T16:33:33Z2024-10-23T16:33:33Z2024-03-29Belinchón-deMiguel, P.; Navarro-Jiménez, E.; LabordeCárdenas, C.C.; Clemente-Suárez, V.J. Evolutionary Echoes: A Four-Day Fasting and Low-Caloric Intake Study on Autonomic Modulation and Physiological Adaptations in Humans. Life 2024, 14, 456. https://doi.org/ 10.3390/life14040456https://hdl.handle.net/11323/1350210.3390/life140404562075-1729Corporación Universidad de la CostaREDICUC - Repositorio CUChttps://repositorio.cuc.edu.co/This study evaluates the psychophysiological response to a simulated hunter–gatherer endurance task with restricted caloric intake over four days. It assesses changes in body composition, autonomic modulation, and physical and cognitive performance. Participants underwent daily 8 h fasted walks followed by a 150 kcal meal to replicate hunter–gatherer activity and dietary patterns. Measurements of metabolic, respiratory, and subjective well-being, along with heart rate variability (HRV) monitoring, were conducted pre- and post-activity to evaluate the impact of endurance activity under caloric restriction. We found weight loss, decreased body and visceral fat, and reduced skeletal muscle mass and water percentage. High sympathetic activation and stable urinary markers, except for increased proteinuria, indicated stress responses and muscular degradation. Elevated perceived exertion post-exercise with good adaptation to prolonged effort underlines the body’s adaptability to ancestral lifestyle conditions, highlighting the connection among endurance, nutrition, and psychophysiological health.15 páginasapplication/pdfengMultidisciplinary Digital Publishing Institute (MDPI)Switzerlandhttps://www.mdpi.com/2075-1729/14/4/456Evolutionary echoes: A four-day fasting and low-caloric intake study on autonomic modulation and physiological adaptations in humansArtículo de revistahttp://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Life1. Di Liegro, C.M.; Schiera, G.; Proia, P.; Di Liegro, I. Physical Activity and Brain Health. Genes 2019, 10, 720. [CrossRef]2. O’Keefe, J.H.; Vogel, R.; Lavie, C.J.; Cordain, L. Exercise Like a Hunter-Gatherer: A Prescription for Organic Physical Fitness. Prog. Cardiovasc. Dis. 2011, 53, 471–479. [CrossRef]3. Wheeler, P. The evolution of bipedality and loss of functional body hair in hominids. J. Hum. Evol. 1984, 13, 91–98. [CrossRef]4. Bramble, D.M.; Lieberman, D.E. Endurance running and the evolution of Homo. Nature 2004, 432, 345–352. [CrossRef]5. Warburton, D.E.R.; Nicol, C.W.; Bredin, S.S.D. Health benefits of physical activity: The evidence. Can. Med. Assoc. J. 2006, 174, 801–809. [CrossRef]6. Clemente-Suárez, V.J. Multidisciplinary intervention in the treatment of mixed anxiety and depression disorder. Physiol. Behav. 2020, 219, 112858. [CrossRef] [PubMed]7. Harris, C.D.; Watson, K.B.; Carlson, S.A.; Fulton, J.E.; Dorn, J.M.; Elam-Evans, L. Adult Participation in Aerobic and MuscleStrengthening Physical Activities—United States, 2011. Morb. Mortal. Wkly. Rep. 2013, 62, 326–330.8. Sponheimer, M.; Alemseged, Z.; Cerling, T.E.; Grine, F.E.; Kimbel, W.H.; Leakey, M.G.; Lee-Thorp, J.A.; Manthi, F.K.; Reed, K.E.; Wood, B.A.; et al. Isotopic evidence of early hominin diets. Proc. Natl. Acad. Sci. USA 2013, 110, 10513–10518. [CrossRef]9. Ungar, P.S.; Sponheimer, M. The Diets of Early Hominins. Science 2011, 334, 190–193. [CrossRef] [PubMed]10. Thompson, J.C.; Carvalho, S.; Marean, C.W.; Alemseged, Z. Origins of the Human Predatory Pattern: The Transition to LargeAnimal Exploitation by Early Hominins. Curr. Anthr. 2019, 60, 1–23. [CrossRef]11. Fontana, L.; Partridge, L. Promoting Health and Longevity through Diet: From Model Organisms to Humans. Cell 2015, 161, 106–118. [CrossRef] [PubMed]12. McMorris, T.; Sproule, J.; Turner, A.; Hale, B.J. Acute, intermediate intensity exercise, and speed and accuracy in working memory tasks: A meta-analytical comparison of effects. Physiol. Behav. 2011, 102, 421–428. [CrossRef]13. Dishman, R.K.; Berthoud, H.; Booth, F.W.; Cotman, C.W.; Edgerton, V.R.; Fleshner, M.R.; Gandevia, S.C.; Gomez-Pinilla, F.; Greenwood, B.N.; Hillman, C.H.; et al. Neurobiology of Exercise. Obesity 2006, 14, 345–356. [CrossRef] [PubMed]14. Messina, A.; Monda, M.; Valenzano, A.; Messina, G.; Villano, I.; Moscatelli, F.; Cibelli, G.; Marsala, G.; Polito, R.; Ruberto, M.; et al. Functional Changes Induced by Orexin A and Adiponectin on the Sympathetic/Parasympathetic Balance. Front. Physiol. 2018, 9, 259. [CrossRef] [PubMed]15. Polito, R.; Valenzano, A.; Scarinci, A.; Villano, I.; Monda, M.; Messina, A.; Cibelli, G.; Porro, C.; La Torre, E.; Pisanelli, D.; et al. Very Low-Calorie Ketogenic Diet Modulates the Autonomic Nervous System Activity through Salivary Amylase in Obese Population Subjects. Int. J. Environ. Res. Public Health 2021, 18, 8475. [CrossRef] [PubMed]16. Mammen, G.; Faulkner, G. Physical activity and the prevention of depression: A systematic review of prospective studies. Am. J. Prev. Med. 2013, 45, 649–657. [CrossRef]17. Erickson, K.I.; Hillman, C.; Stillman, C.M.; Ballard, R.M.; Bloodgood, B.; Conroy, D.E.; Macko, R.; Marquez, D.X.; Petruzzello, S.J.; Powell, K.E.; et al. Physical Activity, Cognition, and Brain Outcomes: A Review of the 2018 Physical Activity Guidelines. Med. Sci. Sports Exerc. 2019, 51, 1242–1251. [CrossRef]18. Clael, S.; Campos, L.F.; Correia, K.L.; de Lucena, J.M.S.; Gentil, P.; Durigan, J.L.; Ribeiro, A.L.d.A.; Martins, W.R. Exercise interventions can improve muscle strength, endurance, and electrical activity of lumbar extensors in individuals with non-specific low back pain: A systematic review with meta-analysis. Sci. Rep. 2021, 11, 16842. [CrossRef]19. Belinchón-Demiguel, P.; Ramos-Campo, D.J.; Clemente-Suárez, V.J. Exploring the Evolutionary Disparities: A Case Study on the Psychophysiological Response to Recreating the Hunter–Gatherer Lifestyle through Physical Activity and Caloric Restriction. Appl. Sci. 2023, 13, 11140. [CrossRef]20. Calbet, J.A.L.; Ponce-González, J.G.; Pérez-Suárez, I.; Herrero, J.d.l.C.; Holmberg, H. A time-efficient reduction of fat mass in 4 days with exercise and caloric restriction. Scand. J. Med. Sci. Sports 2015, 25, 223–233. [CrossRef] [PubMed]21. Arakawa, K.; Hosono, A.; Shibata, K.; Ghadimi, R.; Fuku, M.; Goto, C.; Imaeda, N.; Tokudome, Y.; Hoshino, H.; Marumoto, M.; et al. Changes in blood biochemical markers before, during, and after a 2-day ultramarathon. Open Access J. Sports Med. 2016, 7, 43–50. [CrossRef]22. Korobeynikov, G.; Korobeynikova, L.; Iermakov, S.; Nosko, M. Reaction of Heart Rate Regulation to Extreme Sport Activity in Elite Athletes. 2016. Available online: http://erpub.chnpu.edu.ua:8080/jspui/bitstream/123456789/194/1/Reaction%20of%20heart% 20rate%20regulation%20to%20extreme%20sport%20activity%20in%20elite%20athletes.pdf (accessed on 27 November 2023).23. Kajaia, T.; Maskhulia, L.; Chelidze, K.; Akhalkatsi, V.; Kakhabrishvili, Z. The effects of non-functional overreaching and overtraining on autonomic nervous system function in highly trained Georgian athletes. Georgian Med. Newa 2017, 3, 97–101.24. Belinchón-Demiguel, P.; Tornero-Aguilera, J.F.; Dalamitros, A.A.; Nikolaidis, P.T.; Rosemann, T.; Knechtle, B.; Clemente-Suárez, V.J. Multidisciplinary Analysis of Differences Between Finisher and Non-finisher Ultra-Endurance Mountain Athletes. Front. Physiol. 2019, 10, 1507. [CrossRef]25. Belinchon-Demiguel, P.; Clemente-Suárez, V.J. Psychophysiological, Body Composition, Biomechanical and Autonomic Modulation Analysis Procedures in an Ultraendurance Mountain Race. J. Med. Syst. 2018, 42, 32. [CrossRef] [PubMed]26. Garcia, J.R.; Manimaleth, R.; Czartoryski, P.; Napolitano, P.; Watters, H.; Weber, C.; Alvarez-Beaton, A.; Nieto, A.C.; Patel, A.; Peacock, C. A Comparative Study of Body Composition Assessment Techniques: DXA and InBody 270. J. Exerc. Nutr. 2020, 3, 3.27. Tornero-Aguilera, J.F.; Clemente-Suárez, V.J. Cognitive and psychophysiological impact of surgical mask use during university lessons. Physiol. Behav. 2021, 234, 113342. [CrossRef] [PubMed]28. Borg, G. Perceived exertion as an indicator of somatic stress. J. Rehabil. Med. 1970, 2, 92–98. [CrossRef]29. Willis, E.A.; Creasy, S.A.; Honas, J.J.; Melanson, E.L.; Donnelly, J.E. The effects of exercise session timing on weight loss and components of energy balance: Midwest exercise trial 2. Int. J. Obes. 2020, 44, 114–124. [CrossRef]30. Swift, D.L.; Johannsen, N.M.; Lavie, C.J.; Earnest, C.P.; Church, T.S. The Role of Exercise and Physical Activity in Weight Loss and Maintenance. Prog. Cardiovasc. Dis. 2014, 56, 441–447. [CrossRef]31. Cahill, G.F., Jr. Fuel Metabolism in Starvation. Annu. Rev. Nutr. 2006, 26, 1–22. [CrossRef]32. Phinney, S.; Bistrian, B.; Evans, W.; Gervino, E.; Blackburn, G. The human metabolic response to chronic ketosis without caloric restriction: Preservation of submaximal exercise capability with reduced carbohydrate oxidation. Metabolism 1983, 32, 769–776. [CrossRef]33. Volek, J.S.; Freidenreich, D.J.; Saenz, C.; Kunces, L.J.; Creighton, B.C.; Bartley, J.M.; Davitt, P.M.; Munoz, C.X.; Anderson, J.M.; Maresh, C.M.; et al. Metabolic characteristics of keto-adapted ultra-endurance runners. Metabolism 2016, 65, 100–110. [CrossRef]34. Most, J.; Redman, L.M. Impact of calorie restriction on energy metabolism in humans. Exp. Gerontol. 2020, 133, 110875. [CrossRef]35. Neeland, I.J.; Ross, R.; Després, J.-P.; Matsuzawa, Y.; Yamashita, S.; Shai, I.; Seidell, J.; Magni, P.; Santos, R.D.; Arsenault, B.; et al. Visceral and ectopic fat, atherosclerosis, and cardiometabolic disease: A position statement. Lancet Diabetes Endocrinol. 2019, 7, 715–725. [CrossRef]36. Ismail, I.; Keating, S.E.; Baker, M.K.; Johnson, N.A. A systematic review and meta-analysis of the effect of aerobic vs. resistance exercise training on visceral fat. Obes. Rev. 2012, 13, 68–91. [CrossRef]37. Posadzki, P.; Pieper, D.; Bajpai, R.; Makaruk, H.; Könsgen, N.; Neuhaus, A.L.; Semwal, M. Exercise/physical activity and health outcomes: An overview of Cochrane systematic reviews. BMC Public Health 2020, 20, 1724. [CrossRef]38. You, T.; Murphy, K.M.; Lyles, M.F.; Demons, J.L.; Lenchik, L.; Nicklas, B.J. Addition of aerobic exercise to dietary weight loss preferentially reduces abdominal adipocyte size. Int. J. Obes. 2006, 30, 1211–1216. [CrossRef]39. Basile, A.J.; Renner, M.W.; Hidaka, B.H.; Sweazea, K.L. An evolutionary mismatch narrative to improve lifestyle medicine: A patient education hypothesis. Evol. Med. Public Health 2021, 9, 157–163. [CrossRef]40. Tiller, N.B.; Chiesa, S.T.; Roberts, J.D.; Turner, L.A.; Jones, S.; Romer, L.M. Physiological and Pathophysiological Consequences of a 25-Day Ultra-Endurance Exercise Challenge. Front. Physiol. 2019, 10, 589. [CrossRef] [PubMed]41. Heydenreich, J.; Kayser, B.; Schutz, Y.; Melzer, K. Total Energy Expenditure, Energy Intake, and Body Composition in Endurance Athletes Across the Training Season: A Systematic Review. Sports Med. Open 2017, 3, 8. [CrossRef] [PubMed]42. Wang, X.H.; Mitch, W.E.; Price, S.R. Pathophysiological mechanisms leading to muscle loss in chronic kidney disease. Nat. Rev. Nephrol. 2021, 18, 138–152. [CrossRef]43. Kinrade, E.J.; Galloway, S.D.R. Dietary Observations of Ultra-Endurance Runners in Preparation for and During a Continuous 24-h Event. Front. Physiol. 2021, 12. [CrossRef]44. Kreider, R.B.; Wilborn, C.D.; Taylor, L.; Campbell, B.; Almada, A.L.; Collins, R.; Cooke, M.; Earnest, C.P.; Greenwood, M.; Kalman, D.S.; et al. ISSN exercise & sport nutrition review: Research & recommendations. J. Int. Soc. Sports Nutr. 2010, 7, 7. [CrossRef]45. Hoffman, M.D. Proper Hydration During Ultra-endurance Activities. Sports Med. Arthrosc. Rev. 2019, 27, 8–14. [CrossRef]46. Vicente-Salar, N.; Otegui, A.U.; Collado, E.R. Endurance Training in Fasting Conditions: Biological Adaptations and Body Weight Management. Nutr. Hosp. 2015, 32, 2409–242047. Clemente-Suárez, V.J. Psychophysiological response and energy balance during a 14-h ultraendurance mountain running event. Appl. Physiol. Nutr. Metab. 2015, 40, 269–273. [CrossRef]48. Moore, R.D.; Romine, M.W.; O’Connor, P.J.; Tomporowski, P.D. The influence of exercise-induced fatigue on cognitive function. J. Sports Sci. 2012, 30, 841–850. [CrossRef]49. Wollseiffen, P.; Schneider, S.; Martin, L.A.; Kerhervé, H.A.; Klein, T.; Solomon, C. The effect of 6 h of running on brain activity, mood, and cognitive performance. Exp. Brain Res. 2016, 234, 1829–1836. [CrossRef] [PubMed]50. Pontzer, H. Economy and Endurance in Human Evolution. Curr. Biol. 2017, 27, R613–R621. [CrossRef] [PubMed]51. Pettersen, S.D.; Aslaksen, P.M.; Pettersen, S.A. Pain processing in elite and high-level athletes compared to non-athletes. Front. Psychol. 2020, 11, 1908. [CrossRef] [PubMed]52. Hoffman, M.D.; Fogard, K. Factors Related to Successful Completion of a 161-km Ultramarathon. Int. J. Sports Physiol. Perform. 2011, 6, 25–37. [CrossRef]53. Berger, N.J.A.; Best, R.; Best, A.W.; Lane, A.M.; Millet, G.Y.; Barwood, M.; Marcora, S.; Wilson, P.; Bearden, S. Limits of Ultra: Towards an Interdisciplinary Understanding of Ultra-Endurance Running Performance. Sports Med. 2023, 54, 73–93. [CrossRef] [PubMed]54. Best, R.; Barwick, B.; Best, A.; Berger, N.; Harrison, C.; Wright, M.; Sparrow, J. Changes in Pain and Nutritional Intake Modulate Ultra-Running Performance: A Case Report. Sports 2018, 6, 111. [CrossRef] [PubMed]55. Herbert, B.M.; Pollatos, O. Attenuated interoceptive sensitivity in overweight and obese individuals. Eat. Behav. 2014, 15, 445–448. [CrossRef] [PubMed]56. Martínez-Navarro, I.; Sanchez-Gómez, J.M.; Aparicio, I.; Priego-Quesada, J.I.; Pérez-Soriano, P.; Collado, E.; Hernando, B.; Hernando, C. Effect of mountain ultramarathon distance competition on biochemical variables, respiratory and lower-limb fatigue. PLoS ONE 2020, 15, e0238846. [CrossRef]151414FastingUltraenduranceAutonomic modulationHeart rate variabilityHunter–gatherer dietBody compositionPublicationORIGINALEvolutionary echoes A four-day fasting and low-caloric intake study on autonomic modulation and physiological adaptations in humans.pdfEvolutionary echoes A four-day fasting and low-caloric intake study on autonomic modulation and physiological adaptations in humans.pdfapplication/pdf896396https://repositorio.cuc.edu.co/bitstreams/f25b7511-c647-4ccc-a694-51677bef7d11/downloadf344bafd6013b9b0ce57e9756f813003MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-815543https://repositorio.cuc.edu.co/bitstreams/b259b155-2fec-499e-9bca-bb215436ca68/download73a5432e0b76442b22b026844140d683MD52TEXTEvolutionary echoes A four-day fasting and low-caloric intake study on autonomic modulation and physiological adaptations in humans.pdf.txtEvolutionary echoes A four-day fasting and low-caloric intake study on autonomic modulation and physiological adaptations in humans.pdf.txtExtracted texttext/plain58804https://repositorio.cuc.edu.co/bitstreams/c2bce592-9a44-4113-be28-83c81921f4bc/download495b48d2ea5982cec5ea580eb06792b4MD53THUMBNAILEvolutionary echoes A four-day fasting and low-caloric intake study on autonomic modulation and physiological adaptations in humans.pdf.jpgEvolutionary echoes A four-day fasting and low-caloric intake study on autonomic modulation and physiological adaptations in humans.pdf.jpgGenerated Thumbnailimage/jpeg15805https://repositorio.cuc.edu.co/bitstreams/d39bf461-e5b3-4ded-bb31-d2cb2f67a42f/download16c493afd1b595400a3d7eb327be28dbMD5411323/13502oai:repositorio.cuc.edu.co:11323/135022024-10-24 03:01:18.149https://creativecommons.org/licenses/by/4.0/© 2024 by the authors. 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CUCrepdigital@cuc.edu.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