Determination of Kresoxim-Methyl in Water and in Grapes by High-Performance Liquid Chromatography (HPLC) Using PhotochemicalInduced Fluorescence and Dispersive Liquid-Liquid Microextraction (DLLME)

A high-performance chromatographic method was developed to determine the fungicide kresoxim-methyl. Off-line photochemical derivatization was used to induce the formation of a stable and fluorescent product since the fungicide does not present natural fluorescence. Intense fluorescence at 370/430nm was achieved by treating the analyte in solution at pH 6 to ultraviolet light for 45s. The chromatographic conditions included isocratic elution with 50/ 50% (v/v) acetonitrile/water and the photochemical product appeared at a retention time of 7.2min. The short and long term stabilities of the photoproduct were evaluated and variation of less than 5% was achieved. The limits of detection in water samples and in grapes samples were 0.019mg kg1 and 0.065mg kg1 of kresoxim-methyl residue, respectively. The linear response covered three orders of magnitude up to 10.6mg kg1 of kresoxim-methyl. The robustness was evaluated through a Box–Behnken experimental design showing the insignificance of all factors and their interactions. The potential interference of tebuconazole for the determination of kresoxim-methyl was studied. The use of the dispersive liquid-liquid microextraction (DLLME) allowed recoveries between 80% and 101% depending on concentration with the minimum generation of waste products.

• Autores: T. Toloza, Carlos A.; S. Almeida, Joseany M.; P. Silva, Lillian O.; Macedo, Rosana C.; Lamounier, Ana Paula; Aucelio, Ricardo Q.; da Cunha, Alessandra L. M. C.
• Tipo de recurso: http://purl.org/coar/resource_type/c_816b
• Fecha de publicación: 2020
• Institución: Corporación Universidad de la Costa
• Repositorio: REDICUC - Repositorio CUC
• Idioma: eng
• OAI Identifier: oai:repositorio.cuc.edu.co:11323/6145
• Acceso en línea: https://hdl.handle.net/11323/6145; https://repositorio.cuc.edu.co/
• Palabra clave: Dispersive liquid-liquid microextraction (DLLME); High-performance liquid chromatography (HPLC); Kresoxim-methyl; Photochemical-induced fluorescence; Strobirulin
• Rights: openAccess
• License: CC0 1.0 Universal