Submerged cultivation of Nigrospora sp. in batch and fed-batch modes for microbial oil production
Microbial lipids are a valuable source of potential biofuels and essential polyunsaturated fatty acids. The optimization of the fermentation conditions is a strategy that affects the total lipid concentration. The genus Nigrospora sp. has been the target of investigations based on its potential bioh...
- Autores:
-
Tonato, Denise
Brun, Thiarles
Luft, Luciana
Nascimento dos Santos, Maicon Sérgio
Drumm, Fernanda Caroline
Grassi, Patrícia
georgin, jordana
Kuhn, Raquel C.
Zabot, Giovani L.
Mazutti, Marcio A.
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2023
- Institución:
- Corporación Universidad de la Costa
- Repositorio:
- REDICUC - Repositorio CUC
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.cuc.edu.co:11323/10570
- Acceso en línea:
- https://hdl.handle.net/11323/10570
https://repositorio.cuc.edu.co/
- Palabra clave:
- Agroindustrial residue
Fatty acids
Lipid accumulation
Oleaginous microorganisms
Stirred tank bioreactor
- Rights
- embargoedAccess
- License
- Atribución 4.0 Internacional (CC BY 4.0)
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Submerged cultivation of Nigrospora sp. in batch and fed-batch modes for microbial oil production |
| title |
Submerged cultivation of Nigrospora sp. in batch and fed-batch modes for microbial oil production |
| spellingShingle |
Submerged cultivation of Nigrospora sp. in batch and fed-batch modes for microbial oil production Agroindustrial residue Fatty acids Lipid accumulation Oleaginous microorganisms Stirred tank bioreactor |
| title_short |
Submerged cultivation of Nigrospora sp. in batch and fed-batch modes for microbial oil production |
| title_full |
Submerged cultivation of Nigrospora sp. in batch and fed-batch modes for microbial oil production |
| title_fullStr |
Submerged cultivation of Nigrospora sp. in batch and fed-batch modes for microbial oil production |
| title_full_unstemmed |
Submerged cultivation of Nigrospora sp. in batch and fed-batch modes for microbial oil production |
| title_sort |
Submerged cultivation of Nigrospora sp. in batch and fed-batch modes for microbial oil production |
| dc.creator.fl_str_mv |
Tonato, Denise Brun, Thiarles Luft, Luciana Nascimento dos Santos, Maicon Sérgio Drumm, Fernanda Caroline Grassi, Patrícia georgin, jordana Kuhn, Raquel C. Zabot, Giovani L. Mazutti, Marcio A. |
| dc.contributor.author.none.fl_str_mv |
Tonato, Denise Brun, Thiarles Luft, Luciana Nascimento dos Santos, Maicon Sérgio Drumm, Fernanda Caroline Grassi, Patrícia georgin, jordana Kuhn, Raquel C. Zabot, Giovani L. Mazutti, Marcio A. |
| dc.subject.proposal.eng.fl_str_mv |
Agroindustrial residue Fatty acids Lipid accumulation Oleaginous microorganisms Stirred tank bioreactor |
| topic |
Agroindustrial residue Fatty acids Lipid accumulation Oleaginous microorganisms Stirred tank bioreactor |
| description |
Microbial lipids are a valuable source of potential biofuels and essential polyunsaturated fatty acids. The optimization of the fermentation conditions is a strategy that affects the total lipid concentration. The genus Nigrospora sp. has been the target of investigations based on its potential bioherbicidal action. Therefore, this study developed a strategy to maximize the biomass concentration and lipid accumulation by Nigrospora sp. in submerged fermentation. Different media compositions and process variables were investigated in shaken flasks and bioreactor in batch and fed-batch modes. Maximum biomass concentration and lipid accumulations were 40.17 g/L and 21.32 wt% in the bioreactor, which was 2.1 and 5.4 times higher than the same condition in shaken flasks, respectively. This study presents relevant information to the production of fungal lipids since few investigations are exploring the fed-batch strategy to increase the yield of fungi lipids, as well as few studies investigating Nigrospora sp. to produce lipids. |
| publishDate |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2023-11-01T18:55:10Z |
| dc.date.available.none.fl_str_mv |
2023-11-01T18:55:10Z 2024-05-21 |
| dc.date.issued.none.fl_str_mv |
2023-04-21 |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/redcol/resource_type/ART |
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Denise Tonato, Thiarles Brun, Luciana Luft, Maicon Sérgio Nascimento dos Santos, Fernanda Caroline Drumm, Patrícia Grassi, Jordana Georgin, Raquel C. Kuhn, Giovani L. Zabot & Marcio A. Mazutti (2023) Submerged cultivation of Nigrospora sp. in batch and fed-batch modes for microbial oil production, Natural Product Research, DOI: 10.1080/14786419.2023.2214946 |
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1478-6419 |
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https://hdl.handle.net/11323/10570 |
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10.1080/14786419.2023.2214946 |
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1478-6427 |
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Corporación Universidad de la Costa |
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REDICUC - Repositorio CUC |
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https://repositorio.cuc.edu.co/ |
| identifier_str_mv |
Denise Tonato, Thiarles Brun, Luciana Luft, Maicon Sérgio Nascimento dos Santos, Fernanda Caroline Drumm, Patrícia Grassi, Jordana Georgin, Raquel C. Kuhn, Giovani L. Zabot & Marcio A. Mazutti (2023) Submerged cultivation of Nigrospora sp. in batch and fed-batch modes for microbial oil production, Natural Product Research, DOI: 10.1080/14786419.2023.2214946 1478-6419 10.1080/14786419.2023.2214946 1478-6427 Corporación Universidad de la Costa REDICUC - Repositorio CUC |
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https://hdl.handle.net/11323/10570 https://repositorio.cuc.edu.co/ |
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eng |
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eng |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Natural Product Research |
| dc.relation.references.spa.fl_str_mv |
Chatzifragkou A, Fakas S, Galiotou-Panayotou M, Komaitis M, Aggelis G, Papanikolaou S. 2010. Commercial sugars as substrates for lipid accumulation in Cunninghamella echinulata and Mortierella isabellina fungi. Eur J Lipid Sci Technol. 112(9):1048–1057. Fei Q, O’Brien M, Nelson R, Chen X, Lowell A, Dowe N. 2016. Enhanced lipid production by Rhodosporidium toruloides using different fed-batch feeding strategies with lignocellulosic hydrolysate as the sole carbon source. Biotechnol Biofuels. 9(1):1–12. Galhaup C, Wagner H, Hinterstoisser B, Haltrich D. 2002. Increased production of laccase by the wood-degrading basidiomycete Trametes pubescens. Enzyme Microb Technol. 30(4):529–536. Ivančić M, Grubišić M, Galić Perečinec M, Beluhan S, Šantek B. 2021. Lipid production by Mortierella isabellina from pretreated corn cobs and effect of lignocellulose derived inhibitors on growth and lipid synthesis. Process Biochem. 109:46–58. Karamerou EE, Theodoropoulos C, Webb C. 2017. Evaluating feeding strategies for microbial oil production from glycerol by Rhodotorula glutinis. Eng Life Sci. 17(3):314–324. Ma Y, Gao Z, Wang Q, Liu Y. 2018. Biodiesels from microbial oils: opportunity and challenges. Bioresour Technol. 263:631–641. Mironov AA, Nemashkalov VA, Stepanova NN, Kamzolova SV, Rymowicz W, Morgunov IG. 2018. The effect of ph and temperature on arachidonic acid production by glycerol-grown Mortierella alpina NRRL-A-10995. Fermentation. 4(1):17. Peng XW, Chen HZ. 2007. Microbial oil accumulation and cellulase secretion of the endophytic fungi from oleaginous plants. Ann Microbiol. 57(2):239–242. Saad N, Abdeshahian P, Kalil MS, Wan Yusoff WM, Abdul Hamid A. 2014. Optimization of aeration and agitation rate for lipid and gamma linolenic acid production by Cunninghamella bainieri 2a1 in submerged fermentation using response surface methodology. Sci World J. 2014:1–12. Scheel RA, Ho T, Kageyama Y, Masisak J, McKenney S, Lundgren BR, Nomura CT. 2021. Optimizing a fed-batch high-density fermentation process for medium chain-length poly(3-hydroxyalkanoates) in Escherichia coli. Front Bioeng Biotechnol. 9:1–16. Shaigani P, Awad D, Redai V, Fuchs M, Haack M, Mehlmer N, Brueck T. 2021. Oleaginous yeasts- substrate preference and lipid productivity: a view on the performance of microbial lipid producers. Microb Cell Fact. 20(1):1–18. Subhash GV, Mohan SV. 2014. Lipid accumulation for biodiesel production by oleaginous fungus Aspergillus awamori: influence of critical factors. Fuel. 116:509–515. Sun XM, Geng LJ, Ren LJ, Ji XJ, Hao N, Chen KQ, Huang H. 2018. Influence of oxygen on the biosynthesis of polyunsaturated fatty acids in microalgae. Bioresour Technol. 250:868–876. Tchakouteu SS, Kopsahelis N, Chatzifragkou A, Kalantzi O, Stoforos NG, Koutinas AA, Aggelis G, Papanikolaou S. 2017. Rhodosporidium toruloides cultivated in NaCl-enriched glucose-based media: adaptation dynamics and lipid production. Eng Life Sci. 17(3):237–248. Tonato D, Luft L, Confortin TC, Zabot GL, Mazutti MA. 2019. Enhancement of fatty acids in the oil extracted from the fungus Nigrospora sp. by supercritical CO2 with ethanol as a cosolvent. J Supercrit Fluids. 146:180–188. Tonato D, Marcuz C, Vendruscolo RG, Bevilacqua C, Jacques RJS, Wagner R, Kuhn RC, Mazutti MA. 2018. Production of polyunsaturated fatty acids by microorganisms isolated in the Brazilian Pampa biome. Braz J Chem Eng. 35(3):835–846. Wang L-R, Zhang Z-X, Nong F-T, Li J, Huang P-W, Ma W, Zhao Q-Y, Sun X-M. 2022. Engineering the xylose metabolism in Schizochytrium sp. to improve the utilization of lignocellulose. Biotechnol Biofuels Bioprod. 15(1):114. Zikou E, Chatzifragkou A, Koutinas AA, Papanikolaou S. 2013. Evaluating glucose and xylose as cosubstrates for lipid accumulation and γ-linolenic acid biosynthesis of Thamnidium elegans. J Appl Microbiol. 114(4):1020–1032. [Crossref] [PubMed] [Web of Science ®], [Google Scholar] |
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© 2023 Informa UK Limited |
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Atribución 4.0 Internacional (CC BY 4.0) |
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Atribución 4.0 Internacional (CC BY 4.0)© 2023 Informa UK Limitedhttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/embargoedAccesshttp://purl.org/coar/access_right/c_f1cfTonato, DeniseBrun, ThiarlesLuft, LucianaNascimento dos Santos, Maicon SérgioDrumm, Fernanda CarolineGrassi, Patríciageorgin, jordanaKuhn, Raquel C.Zabot, Giovani L.Mazutti, Marcio A.2023-11-01T18:55:10Z2024-05-212023-11-01T18:55:10Z2023-04-21Denise Tonato, Thiarles Brun, Luciana Luft, Maicon Sérgio Nascimento dos Santos, Fernanda Caroline Drumm, Patrícia Grassi, Jordana Georgin, Raquel C. Kuhn, Giovani L. Zabot & Marcio A. Mazutti (2023) Submerged cultivation of Nigrospora sp. in batch and fed-batch modes for microbial oil production, Natural Product Research, DOI: 10.1080/14786419.2023.22149461478-6419https://hdl.handle.net/11323/1057010.1080/14786419.2023.22149461478-6427Corporación Universidad de la CostaREDICUC - Repositorio CUChttps://repositorio.cuc.edu.co/Microbial lipids are a valuable source of potential biofuels and essential polyunsaturated fatty acids. The optimization of the fermentation conditions is a strategy that affects the total lipid concentration. The genus Nigrospora sp. has been the target of investigations based on its potential bioherbicidal action. Therefore, this study developed a strategy to maximize the biomass concentration and lipid accumulation by Nigrospora sp. in submerged fermentation. Different media compositions and process variables were investigated in shaken flasks and bioreactor in batch and fed-batch modes. Maximum biomass concentration and lipid accumulations were 40.17 g/L and 21.32 wt% in the bioreactor, which was 2.1 and 5.4 times higher than the same condition in shaken flasks, respectively. This study presents relevant information to the production of fungal lipids since few investigations are exploring the fed-batch strategy to increase the yield of fungi lipids, as well as few studies investigating Nigrospora sp. to produce lipids.1 páginaapplication/pdfengTaylor and Francis Ltd.United Kingdomhttps://www.tandfonline.com/doi/abs/10.1080/14786419.2023.2214946Submerged cultivation of Nigrospora sp. in batch and fed-batch modes for microbial oil productionArtículo de revistahttp://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/drafthttp://purl.org/coar/version/c_b1a7d7d4d402bcceNatural Product ResearchChatzifragkou A, Fakas S, Galiotou-Panayotou M, Komaitis M, Aggelis G, Papanikolaou S. 2010. Commercial sugars as substrates for lipid accumulation in Cunninghamella echinulata and Mortierella isabellina fungi. Eur J Lipid Sci Technol. 112(9):1048–1057.Fei Q, O’Brien M, Nelson R, Chen X, Lowell A, Dowe N. 2016. Enhanced lipid production by Rhodosporidium toruloides using different fed-batch feeding strategies with lignocellulosic hydrolysate as the sole carbon source. Biotechnol Biofuels. 9(1):1–12.Galhaup C, Wagner H, Hinterstoisser B, Haltrich D. 2002. Increased production of laccase by the wood-degrading basidiomycete Trametes pubescens. Enzyme Microb Technol. 30(4):529–536.Ivančić M, Grubišić M, Galić Perečinec M, Beluhan S, Šantek B. 2021. Lipid production by Mortierella isabellina from pretreated corn cobs and effect of lignocellulose derived inhibitors on growth and lipid synthesis. Process Biochem. 109:46–58.Karamerou EE, Theodoropoulos C, Webb C. 2017. Evaluating feeding strategies for microbial oil production from glycerol by Rhodotorula glutinis. Eng Life Sci. 17(3):314–324.Ma Y, Gao Z, Wang Q, Liu Y. 2018. Biodiesels from microbial oils: opportunity and challenges. Bioresour Technol. 263:631–641.Mironov AA, Nemashkalov VA, Stepanova NN, Kamzolova SV, Rymowicz W, Morgunov IG. 2018. The effect of ph and temperature on arachidonic acid production by glycerol-grown Mortierella alpina NRRL-A-10995. Fermentation. 4(1):17.Peng XW, Chen HZ. 2007. Microbial oil accumulation and cellulase secretion of the endophytic fungi from oleaginous plants. Ann Microbiol. 57(2):239–242.Saad N, Abdeshahian P, Kalil MS, Wan Yusoff WM, Abdul Hamid A. 2014. Optimization of aeration and agitation rate for lipid and gamma linolenic acid production by Cunninghamella bainieri 2a1 in submerged fermentation using response surface methodology. Sci World J. 2014:1–12.Scheel RA, Ho T, Kageyama Y, Masisak J, McKenney S, Lundgren BR, Nomura CT. 2021. Optimizing a fed-batch high-density fermentation process for medium chain-length poly(3-hydroxyalkanoates) in Escherichia coli. Front Bioeng Biotechnol. 9:1–16.Shaigani P, Awad D, Redai V, Fuchs M, Haack M, Mehlmer N, Brueck T. 2021. Oleaginous yeasts- substrate preference and lipid productivity: a view on the performance of microbial lipid producers. Microb Cell Fact. 20(1):1–18.Subhash GV, Mohan SV. 2014. Lipid accumulation for biodiesel production by oleaginous fungus Aspergillus awamori: influence of critical factors. Fuel. 116:509–515.Sun XM, Geng LJ, Ren LJ, Ji XJ, Hao N, Chen KQ, Huang H. 2018. Influence of oxygen on the biosynthesis of polyunsaturated fatty acids in microalgae. Bioresour Technol. 250:868–876.Tchakouteu SS, Kopsahelis N, Chatzifragkou A, Kalantzi O, Stoforos NG, Koutinas AA, Aggelis G, Papanikolaou S. 2017. Rhodosporidium toruloides cultivated in NaCl-enriched glucose-based media: adaptation dynamics and lipid production. Eng Life Sci. 17(3):237–248.Tonato D, Luft L, Confortin TC, Zabot GL, Mazutti MA. 2019. Enhancement of fatty acids in the oil extracted from the fungus Nigrospora sp. by supercritical CO2 with ethanol as a cosolvent. J Supercrit Fluids. 146:180–188.Tonato D, Marcuz C, Vendruscolo RG, Bevilacqua C, Jacques RJS, Wagner R, Kuhn RC, Mazutti MA. 2018. Production of polyunsaturated fatty acids by microorganisms isolated in the Brazilian Pampa biome. Braz J Chem Eng. 35(3):835–846.Wang L-R, Zhang Z-X, Nong F-T, Li J, Huang P-W, Ma W, Zhao Q-Y, Sun X-M. 2022. Engineering the xylose metabolism in Schizochytrium sp. to improve the utilization of lignocellulose. Biotechnol Biofuels Bioprod. 15(1):114.Zikou E, Chatzifragkou A, Koutinas AA, Papanikolaou S. 2013. Evaluating glucose and xylose as cosubstrates for lipid accumulation and γ-linolenic acid biosynthesis of Thamnidium elegans. J Appl Microbiol. 114(4):1020–1032. [Crossref] [PubMed] [Web of Science ®], [Google Scholar]Agroindustrial residueFatty acidsLipid accumulationOleaginous microorganismsStirred tank bioreactorPublicationORIGINALSubmerged cultivation of Nigrospora sp. in batch and fed-batch modes for microbial oil production.pdfSubmerged cultivation of Nigrospora sp. in batch and fed-batch modes for microbial oil production.pdfArtículoapplication/pdf77595https://repositorio.cuc.edu.co/bitstreams/2dc450e6-1a44-4599-8916-f0a19755852f/downloadd84b07335e8abf4c45c6c3d4a00576bcMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-814828https://repositorio.cuc.edu.co/bitstreams/62632475-0568-402b-8395-507793287460/download2f9959eaf5b71fae44bbf9ec84150c7aMD52TEXTSubmerged cultivation of Nigrospora sp. in batch and fed-batch modes for microbial oil production.pdf.txtSubmerged cultivation of Nigrospora sp. in batch and fed-batch modes for microbial oil production.pdf.txtExtracted 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ada en las Obras Colectivas.

b.	Distribuir copias o fonogramas de las Obras, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública, incluyéndolas como incorporadas en Obras Colectivas, según corresponda.

c.	Distribuir copias de las Obras Derivadas que se generen, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública.
Los derechos mencionados anteriormente pueden ser ejercidos en todos los medios y formatos, actualmente conocidos o que se inventen en el futuro. Los derechos antes mencionados incluyen el derecho a realizar dichas modificaciones en la medida que sean técnicamente necesarias para ejercer los derechos en otro medio o formatos, pero de otra manera usted no está autorizado para realizar obras derivadas. Todos los derechos no otorgados expresamente por el Licenciante quedan por este medio reservados, incluyendo pero sin limitarse a aquellos que se mencionan en las secciones 4(d) y 4(e).

4. Restricciones.
La licencia otorgada en la anterior Sección 3 está expresamente sujeta y limitada por las siguientes restricciones:

a.	Usted puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra sólo bajo las condiciones de esta Licencia, y Usted debe incluir una copia de esta licencia o del Identificador Universal de Recursos de la misma con cada copia de la Obra que distribuya, exhiba públicamente, ejecute públicamente o ponga a disposición pública. No es posible ofrecer o imponer ninguna condición sobre la Obra que altere o limite las condiciones de esta Licencia o el ejercicio de los derechos de los destinatarios otorgados en este documento. No es posible sublicenciar la Obra. Usted debe mantener intactos todos los avisos que hagan referencia a esta Licencia y a la cláusula de limitación de garantías. Usted no puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra con alguna medida tecnológica que controle el acceso o la utilización de ella de una forma que sea inconsistente con las condiciones de esta Licencia. Lo anterior se aplica a la Obra incorporada a una Obra Colectiva, pero esto no exige que la Obra Colectiva aparte de la obra misma quede sujeta a las condiciones de esta Licencia. Si Usted crea una Obra Colectiva, previo aviso de cualquier Licenciante debe, en la medida de lo posible, eliminar de la Obra Colectiva cualquier referencia a dicho Licenciante o al Autor Original, según lo solicitado por el Licenciante y conforme lo exige la cláusula 4(c).

b.	Usted no puede ejercer ninguno de los derechos que le han sido otorgados en la Sección 3 precedente de modo que estén principalmente destinados o directamente dirigidos a conseguir un provecho comercial o una compensación monetaria privada. El intercambio de la Obra por otras obras protegidas por derechos de autor, ya sea a través de un sistema para compartir archivos digitales (digital file-sharing) o de cualquier otra manera no será considerado como estar destinado principalmente o dirigido directamente a conseguir un provecho comercial o una compensación monetaria privada, siempre que no se realice un pago mediante una compensación monetaria en relación con el intercambio de obras protegidas por el derecho de autor.

c.	Si usted distribuye, exhibe públicamente, ejecuta públicamente o ejecuta públicamente en forma digital la Obra o cualquier Obra Derivada u Obra Colectiva, Usted debe mantener intacta toda la información de derecho de autor de la Obra y proporcionar, de forma razonable según el medio o manera que Usted esté utilizando: (i) el nombre del Autor Original si está provisto (o seudónimo, si fuere aplicable), y/o (ii) el nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.

d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:

i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.

ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, los consagrados por la SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.

e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, ACINPRO), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.

5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.

7. Término.

a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.

b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.

8. Varios.

a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.

b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.

c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.

d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.
 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