Oxidative stress biomarkers as prognostic indicators of severity in patients with dengue

There is evidence for the role of oxidative stress in severe dengue pathogenesis. However, previous observational studies presents certain methodological limitations, which may affect its internal and external validity. This study was a case-control analysis of patients with severe dengue and dengue...

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Autores:: Gasparotto, Juciano
Figueiró, Fabrício
de Fraga Dias, Amanda
Rostirolla, Diana Carolina
Somensi, Nauana
Taisda Rosa, Helen
Kich Grun, Lucas
Barbé Tuana, Florencia María
de Miranda Ramos, Vitor
Pens Gelain, Daniel
Fonseca Moreira, José Cláudio
Castro-Orozco, Raimundo
Marcelo, Adolfo
Garcia, María Paquita
Merino, Nancy
Escalante-Maldonado, Oscar
Mora, Cinthya
Rodriguez, Marlon
Alvis-Guzman, Nelson

Tipo de recurso:: Article of journal

Fecha de publicación:: 2018

Institución:: Corporación Universidad de la Costa

Repositorio:: REDICUC - Repositorio CUC

Idioma:: eng

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repository_id_str
dc.title.spa.fl_str_mv	Oxidative stress biomarkers as prognostic indicators of severity in patients with dengue
dc.title.translated.spa.fl_str_mv	Biomarcadores de estrés oxidativo como indicadores pronósticos de severidad en pacientes con dengue
title	Oxidative stress biomarkers as prognostic indicators of severity in patients with dengue
spellingShingle	Oxidative stress biomarkers as prognostic indicators of severity in patients with dengue Dengue Severe dengue Oxidative stress Protein carbonylation Superoxide dismutase Manganese Superoxide dismutase Cu-Zn Lipid hydroperoxide Prognosis Dengue Dengue severo Estrés oxidativo Carbonilación de proteínas Superóxido dismutasa Manganeso Superóxido dismutasa Cu-Zn Hidroperóxido de lípidos Pronóstico
title_short	Oxidative stress biomarkers as prognostic indicators of severity in patients with dengue
title_full	Oxidative stress biomarkers as prognostic indicators of severity in patients with dengue
title_fullStr	Oxidative stress biomarkers as prognostic indicators of severity in patients with dengue
title_full_unstemmed	Oxidative stress biomarkers as prognostic indicators of severity in patients with dengue
title_sort	Oxidative stress biomarkers as prognostic indicators of severity in patients with dengue
dc.creator.fl_str_mv	Gasparotto, Juciano Figueiró, Fabrício de Fraga Dias, Amanda Rostirolla, Diana Carolina Somensi, Nauana Taisda Rosa, Helen Kich Grun, Lucas Barbé Tuana, Florencia María de Miranda Ramos, Vitor Pens Gelain, Daniel Fonseca Moreira, José Cláudio Castro-Orozco, Raimundo Marcelo, Adolfo Garcia, María Paquita Merino, Nancy Escalante-Maldonado, Oscar Mora, Cinthya Rodriguez, Marlon Alvis-Guzman, Nelson
dc.contributor.author.spa.fl_str_mv	Gasparotto, Juciano Figueiró, Fabrício de Fraga Dias, Amanda Rostirolla, Diana Carolina Somensi, Nauana Taisda Rosa, Helen Kich Grun, Lucas Barbé Tuana, Florencia María de Miranda Ramos, Vitor Pens Gelain, Daniel Fonseca Moreira, José Cláudio Castro-Orozco, Raimundo Marcelo, Adolfo Garcia, María Paquita Merino, Nancy Escalante-Maldonado, Oscar Mora, Cinthya Rodriguez, Marlon Alvis-Guzman, Nelson
dc.subject.spa.fl_str_mv	Dengue Severe dengue Oxidative stress Protein carbonylation Superoxide dismutase Manganese Superoxide dismutase Cu-Zn Lipid hydroperoxide Prognosis Dengue Dengue severo Estrés oxidativo Carbonilación de proteínas Superóxido dismutasa Manganeso Superóxido dismutasa Cu-Zn Hidroperóxido de lípidos Pronóstico
topic	Dengue Severe dengue Oxidative stress Protein carbonylation Superoxide dismutase Manganese Superoxide dismutase Cu-Zn Lipid hydroperoxide Prognosis Dengue Dengue severo Estrés oxidativo Carbonilación de proteínas Superóxido dismutasa Manganeso Superóxido dismutasa Cu-Zn Hidroperóxido de lípidos Pronóstico
description	There is evidence for the role of oxidative stress in severe dengue pathogenesis. However, previous observational studies presents certain methodological limitations, which may affect its internal and external validity. This study was a case-control analysis of patients with severe dengue and dengue with warning signs, to evaluate the serum protein carbonyls-PCOs and lipid hydroperoxides-LOOHs levels and activities of superoxide dismutases-SODs (MnSOD, Cu/ZnSOD and total SOD), as potential prognosis indicators of severity in dengue patients, using binary logistic regression analysis and strategy of double cross-validation. Therefore, the study population was subdivided into a derivation group (pediatric patients, Barranquilla-Colombia) and an external validation group (children and adults patients, National Institute of Health of Peru). PCOs was the only oxidative stress markers that showed a strongest association with the severity of dengue, both in children and adults. In the derivation group, the optimal cut-off point was estimated at 5.29 nmol/mg of protein, and in the external validation group, it was 5.77 nmol/mg of protein. The prognostic models based on these two diagnostic thresholds showed a high discriminatory capacity of dengue severity, external reproducibility, geographic transportability, and typical characteristics of diagnostic validity and safety of screening tests.
publishDate	2018
dc.date.issued.none.fl_str_mv	2018-11-08
dc.date.accessioned.none.fl_str_mv	2019-05-24T13:27:10Z
dc.date.available.none.fl_str_mv	2019-05-24T13:27:10Z
dc.type.spa.fl_str_mv	Artículo de revista
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dc.type.content.spa.fl_str_mv	Text
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dc.identifier.issn.spa.fl_str_mv	1916-9736
dc.identifier.uri.spa.fl_str_mv	https://hdl.handle.net/11323/4693
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identifier_str_mv	1916-9736 Corporación Universidad de la Costa REDICUC - Repositorio CUC
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dc.language.iso.none.fl_str_mv	eng
language	eng
dc.relation.ispartof.spa.fl_str_mv	doi:10.5539/gjhs.v11n1p46
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spelling	Gasparotto, JucianoFigueiró, Fabríciode Fraga Dias, AmandaRostirolla, Diana CarolinaSomensi, NauanaTaisda Rosa, HelenKich Grun, LucasBarbé Tuana, Florencia Maríade Miranda Ramos, VitorPens Gelain, DanielFonseca Moreira, José CláudioCastro-Orozco, RaimundoMarcelo, AdolfoGarcia, María PaquitaMerino, NancyEscalante-Maldonado, OscarMora, CinthyaRodriguez, MarlonAlvis-Guzman, Nelson2019-05-24T13:27:10Z2019-05-24T13:27:10Z2018-11-081916-9736https://hdl.handle.net/11323/4693Corporación Universidad de la CostaREDICUC - Repositorio CUChttps://repositorio.cuc.edu.co/There is evidence for the role of oxidative stress in severe dengue pathogenesis. However, previous observational studies presents certain methodological limitations, which may affect its internal and external validity. This study was a case-control analysis of patients with severe dengue and dengue with warning signs, to evaluate the serum protein carbonyls-PCOs and lipid hydroperoxides-LOOHs levels and activities of superoxide dismutases-SODs (MnSOD, Cu/ZnSOD and total SOD), as potential prognosis indicators of severity in dengue patients, using binary logistic regression analysis and strategy of double cross-validation. Therefore, the study population was subdivided into a derivation group (pediatric patients, Barranquilla-Colombia) and an external validation group (children and adults patients, National Institute of Health of Peru). PCOs was the only oxidative stress markers that showed a strongest association with the severity of dengue, both in children and adults. In the derivation group, the optimal cut-off point was estimated at 5.29 nmol/mg of protein, and in the external validation group, it was 5.77 nmol/mg of protein. The prognostic models based on these two diagnostic thresholds showed a high discriminatory capacity of dengue severity, external reproducibility, geographic transportability, and typical characteristics of diagnostic validity and safety of screening tests.Existe evidencia del papel del estrés oxidativo en la patogénesis grave del dengue. Sin embargo, estudios observacionales previos presentan ciertas limitaciones metodológicas, que pueden afectar su validez interna y externa. Este estudio fue un análisis de casos y controles de pacientes con dengue severo y dengue con signos de advertencia, para evaluar la proteína sérica carbonilo-PCO y los niveles de hidroperóxidos de lípidos-LOOH y las actividades de superóxido dismutasas-SOD (MnSOD, Cu / ZnSOD y SOD total) , como posibles indicadores de pronóstico de severidad en pacientes con dengue, mediante análisis de regresión logística binaria y estrategia de doble validación cruzada. Por lo tanto, la población de estudio se subdividió en un grupo de derivación (pacientes pediátricos, Barranquilla-Colombia) y un grupo de validación externa (pacientes niños y adultos, Instituto Nacional de Salud del Perú). Los PCO fueron los únicos marcadores de estrés oxidativo que mostraron una asociación más fuerte con la gravedad del dengue, tanto en niños como en adultos. En el grupo de derivación, el punto de corte óptimo se estimó en 5,29 nmol / mg de proteína, y en el grupo de validación externa fue de 5,77 nmol / mg de proteína. Los modelos de pronóstico basados en estos dos umbrales diagnósticos mostraron una alta capacidad discriminatoria de la gravedad del dengue, la reproducibilidad externa, la transportabilidad geográfica y las características típicas de la validez diagnóstica y la seguridad de las pruebas de detección.Castro-Orozco, Raimundo-df685b87-11b8-481f-93ac-f0cef175462a-0Marcelo, Adolfo-9ce97d57-9383-4396-ba73-69d0607abc52-0Garcia, María Paquita-e6293847-ae41-4287-a5ad-6653bc00576b-0Merino, Nancy-67924017-07b2-4624-9457-ff301654b3cf-0Escalante-Maldonado, Oscar-ff4498ce-c5f1-4667-b41d-75e943a13432-0Mora, Cinthya-6248c1d4-006e-413a-aff2-be6138388b3f-0Rodriguez, Marlon-1ebf48df-ca65-4be6-bcd4-b536e5a9b0cf-0Alvis-Guzman, Nelson-8bd90d52-51ab-4917-ad57-b2424f106eac-0engGlobal Journal of Health Sciencedoi:10.5539/gjhs.v11n1p46Alera, M. T., Srikiatkhachorn, A., Velasco, J. M., Tac-An, I. A., Lago, C. B., Clapham, H. E., . . . Yoon, I.-K. (2016). 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PLoS Negl Trop Dis, 8(6), e2858. https://doi.org/10.1371/journal.pntd.0002858Attribution-NonCommercial-ShareAlike 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2DengueSevere dengueOxidative stressProtein carbonylationSuperoxide dismutaseManganeseSuperoxide dismutaseCu-ZnLipid hydroperoxidePrognosisDengueDengue severoEstrés oxidativoCarbonilación de proteínasSuperóxido dismutasaManganesoSuperóxido dismutasaCu-ZnHidroperóxido de lípidosPronósticoOxidative stress biomarkers as prognostic indicators of severity in patients with dengueBiomarcadores de estrés oxidativo como indicadores pronósticos de severidad en pacientes con dengueArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/acceptedVersionPublicationORIGINALOxidative Stress Biomarkers as Prognostic Indicators of Severity in.pdfOxidative Stress Biomarkers as Prognostic Indicators of Severity in.pdfapplication/pdf337613https://repositorio.cuc.edu.co/bitstreams/11213782-5402-4b21-9ee1-e277e34bfb34/downloadc459bcbc76ad72e68abd8c5ea0ef9ca5MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81031https://repositorio.cuc.edu.co/bitstreams/ca89de39-92d5-4823-9ade-c14d2d6921b8/download934f4ca17e109e0a05eaeaba504d7ce4MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.cuc.edu.co/bitstreams/870cdcab-bf18-4423-9757-41e83ee0ff8c/download8a4605be74aa9ea9d79846c1fba20a33MD53THUMBNAILOxidative Stress Biomarkers as Prognostic Indicators of Severity in.pdf.jpgOxidative Stress Biomarkers as Prognostic Indicators of Severity in.pdf.jpgimage/jpeg65293https://repositorio.cuc.edu.co/bitstreams/e46150e9-e62e-44bd-b246-685a6fa6b87b/download206dbcb5b507371ecc00ae2eef8246acMD55TEXTOxidative Stress Biomarkers as Prognostic Indicators of Severity in.pdf.txtOxidative Stress Biomarkers as Prognostic Indicators of Severity in.pdf.txttext/plain65652https://repositorio.cuc.edu.co/bitstreams/ec21a7c4-c9fe-417e-9a34-8c2e817c37d7/download2bbf166c84aa60c2aaeb5482afdd29caMD5611323/4693oai:repositorio.cuc.edu.co:11323/46932024-09-17 10:47:09.269http://creativecommons.org/licenses/by-nc-sa/4.0/Attribution-NonCommercial-ShareAlike 4.0 Internationalopen.accesshttps://repositorio.cuc.edu.coRepositorio de la Universidad de la Costa CUCrepdigital@cuc.edu.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

Oxidative stress biomarkers as prognostic indicators of severity in patients with dengue

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