A new animal diet based on human western diet is a robust diet-induced obesity model: comparison to high-fat and cafeteria diets in term of metabolic and gut microbiota disruption

Background/Objectives:Obesity is a metabolic disorder that predisposes patients to numerous diseases and has become a major global public-health concern. Animal models of diet-induced obesity (DIO) are frequently used to study obesity, but which DIO model most accurately reflects the pathology of hu...

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Autores:
Calixto Bortolin, Rafael
Rodrigues Vargas, Amanda
Gasparotto, Juciano
Rodrigues Chaves, Paloma
Schnorr, Carlos Eduardo
Da Boit Martinello, Katia
Kleber Silveira, Alexandre
Rabelo, Thallita Kelly
Gelain, D. P
Moreira Fonseca, Jose Claudio
Tipo de recurso:
Article of journal
Fecha de publicación:
2018
Institución:
Corporación Universidad de la Costa
Repositorio:
REDICUC - Repositorio CUC
Idioma:
eng
OAI Identifier:
oai:repositorio.cuc.edu.co:11323/4675
Acceso en línea:
https://hdl.handle.net/11323/4675
https://repositorio.cuc.edu.co/
Palabra clave:
Animal experiment
Animal model
Article
Cafeteria diet
Comorbidity
Comparative study
Controlled study
Diet
Diet induced obesity
Dysbiosis
Food preference
Intestine flora
Lipid diet
Low fat diet
Male
Metabolic parameters
Nonhuman
Phenotype
Priority journal
Rat
Western diet
Experimento animal
Modelo animal
Artículo
Dieta de la cafeteria
Comorbilidad
Estudio comparativo
Estudio controlado
Dieta
Dieta inducida por la obesidad
Disbiosis
Preferencia de alimentos
Flora intestinal
Dieta lipídica
Dieta baja en grasas
Masculino
Parámetros metabolicos
No humano
Fenotipo
Revista de prioridad
Rata
Dieta occidental
Rights
openAccess
License
Attribution-NonCommercial-ShareAlike 4.0 International
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repository_id_str
dc.title.spa.fl_str_mv A new animal diet based on human western diet is a robust diet-induced obesity model: comparison to high-fat and cafeteria diets in term of metabolic and gut microbiota disruption
dc.title.translated.spa.fl_str_mv Una nueva dieta animal basada en la dieta occidental humana es un modelo robusto de obesidad inducida por la dieta: comparación con las dietas ricas en grasas y en la cafetería en términos de alteración metabólica y microbiota intestinal
title A new animal diet based on human western diet is a robust diet-induced obesity model: comparison to high-fat and cafeteria diets in term of metabolic and gut microbiota disruption
spellingShingle A new animal diet based on human western diet is a robust diet-induced obesity model: comparison to high-fat and cafeteria diets in term of metabolic and gut microbiota disruption
Animal experiment
Animal model
Article
Cafeteria diet
Comorbidity
Comparative study
Controlled study
Diet
Diet induced obesity
Dysbiosis
Food preference
Intestine flora
Lipid diet
Low fat diet
Male
Metabolic parameters
Nonhuman
Phenotype
Priority journal
Rat
Western diet
Experimento animal
Modelo animal
Artículo
Dieta de la cafeteria
Comorbilidad
Estudio comparativo
Estudio controlado
Dieta
Dieta inducida por la obesidad
Disbiosis
Preferencia de alimentos
Flora intestinal
Dieta lipídica
Dieta baja en grasas
Masculino
Parámetros metabolicos
No humano
Fenotipo
Revista de prioridad
Rata
Dieta occidental
title_short A new animal diet based on human western diet is a robust diet-induced obesity model: comparison to high-fat and cafeteria diets in term of metabolic and gut microbiota disruption
title_full A new animal diet based on human western diet is a robust diet-induced obesity model: comparison to high-fat and cafeteria diets in term of metabolic and gut microbiota disruption
title_fullStr A new animal diet based on human western diet is a robust diet-induced obesity model: comparison to high-fat and cafeteria diets in term of metabolic and gut microbiota disruption
title_full_unstemmed A new animal diet based on human western diet is a robust diet-induced obesity model: comparison to high-fat and cafeteria diets in term of metabolic and gut microbiota disruption
title_sort A new animal diet based on human western diet is a robust diet-induced obesity model: comparison to high-fat and cafeteria diets in term of metabolic and gut microbiota disruption
dc.creator.fl_str_mv Calixto Bortolin, Rafael
Rodrigues Vargas, Amanda
Gasparotto, Juciano
Rodrigues Chaves, Paloma
Schnorr, Carlos Eduardo
Da Boit Martinello, Katia
Kleber Silveira, Alexandre
Rabelo, Thallita Kelly
Gelain, D. P
Moreira Fonseca, Jose Claudio
dc.contributor.author.spa.fl_str_mv Calixto Bortolin, Rafael
Rodrigues Vargas, Amanda
Gasparotto, Juciano
Rodrigues Chaves, Paloma
Schnorr, Carlos Eduardo
Da Boit Martinello, Katia
Kleber Silveira, Alexandre
Rabelo, Thallita Kelly
Gelain, D. P
Moreira Fonseca, Jose Claudio
dc.subject.spa.fl_str_mv Animal experiment
Animal model
Article
Cafeteria diet
Comorbidity
Comparative study
Controlled study
Diet
Diet induced obesity
Dysbiosis
Food preference
Intestine flora
Lipid diet
Low fat diet
Male
Metabolic parameters
Nonhuman
Phenotype
Priority journal
Rat
Western diet
Experimento animal
Modelo animal
Artículo
Dieta de la cafeteria
Comorbilidad
Estudio comparativo
Estudio controlado
Dieta
Dieta inducida por la obesidad
Disbiosis
Preferencia de alimentos
Flora intestinal
Dieta lipídica
Dieta baja en grasas
Masculino
Parámetros metabolicos
No humano
Fenotipo
Revista de prioridad
Rata
Dieta occidental
topic Animal experiment
Animal model
Article
Cafeteria diet
Comorbidity
Comparative study
Controlled study
Diet
Diet induced obesity
Dysbiosis
Food preference
Intestine flora
Lipid diet
Low fat diet
Male
Metabolic parameters
Nonhuman
Phenotype
Priority journal
Rat
Western diet
Experimento animal
Modelo animal
Artículo
Dieta de la cafeteria
Comorbilidad
Estudio comparativo
Estudio controlado
Dieta
Dieta inducida por la obesidad
Disbiosis
Preferencia de alimentos
Flora intestinal
Dieta lipídica
Dieta baja en grasas
Masculino
Parámetros metabolicos
No humano
Fenotipo
Revista de prioridad
Rata
Dieta occidental
description Background/Objectives:Obesity is a metabolic disorder that predisposes patients to numerous diseases and has become a major global public-health concern. Animal models of diet-induced obesity (DIO) are frequently used to study obesity, but which DIO model most accurately reflects the pathology of human obesity remains unclear. In this study, we designed a diet based on the human Western diet (WD) and compared it with the cafeteria diet (CAF) and high-fat diet (HFD) in order to evaluate which diet most closely mirrors human obesity.Methods:Wistar rats were fed four different diets (WD, CAF, HFD and a low-fat diet) for 18 weeks. Metabolic parameters and gut microbiota changes were then characterized.Results:Rats fed the four different diets exhibited completely different phenotypes, highlighting the importance of diet selection. This study also revealed that WD most effectively induced obesity and obesity-related disorders, and thus proved to be a robust model of human obesity. Moreover, WD-fed rats developed obesity and obesity-related comorbidities independent of major alterations in gut microbiota composition (dysbiosis), whereas CAF-fed rats developed the greatest dysbiosis independent of obesity. We also characterized gut microbiota after feeding on these four different diets and identified five genera that might be involved in the pathogenesis of obesity.Conclusions:These data suggest that diet, and not the obese state, was the major driving force behind gut microbiota changes. Moreover, the marked dysbiosis observed in CAF-fed rats might have resulted from the presence of several additives present in the CAF diet, or even a lack of essential vitamins and minerals. Based on our findings, we recommend the use of the prototypic WD (designed here) in DIO models. Conversely, CAF could be used to investigate the effects of excessive consumption of industrially produced and highly processed foods, which are characteristic of Western society.
publishDate 2018
dc.date.issued.none.fl_str_mv 2018
dc.date.accessioned.none.fl_str_mv 2019-05-22T13:11:34Z
dc.date.available.none.fl_str_mv 2019-05-22T13:11:34Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.identifier.issn.spa.fl_str_mv 03070565
dc.identifier.uri.spa.fl_str_mv https://hdl.handle.net/11323/4675
dc.identifier.instname.spa.fl_str_mv Corporación Universidad de la Costa
dc.identifier.reponame.spa.fl_str_mv REDICUC - Repositorio CUC
dc.identifier.repourl.spa.fl_str_mv https://repositorio.cuc.edu.co/
identifier_str_mv 03070565
Corporación Universidad de la Costa
REDICUC - Repositorio CUC
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dc.language.iso.none.fl_str_mv eng
language eng
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dc.publisher.spa.fl_str_mv Universidad de la Costa
institution Corporación Universidad de la Costa
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spelling Calixto Bortolin, RafaelRodrigues Vargas, AmandaGasparotto, JucianoRodrigues Chaves, PalomaSchnorr, Carlos EduardoDa Boit Martinello, KatiaKleber Silveira, AlexandreRabelo, Thallita KellyGelain, D. PMoreira Fonseca, Jose Claudio2019-05-22T13:11:34Z2019-05-22T13:11:34Z201803070565https://hdl.handle.net/11323/4675Corporación Universidad de la CostaREDICUC - Repositorio CUChttps://repositorio.cuc.edu.co/Background/Objectives:Obesity is a metabolic disorder that predisposes patients to numerous diseases and has become a major global public-health concern. Animal models of diet-induced obesity (DIO) are frequently used to study obesity, but which DIO model most accurately reflects the pathology of human obesity remains unclear. In this study, we designed a diet based on the human Western diet (WD) and compared it with the cafeteria diet (CAF) and high-fat diet (HFD) in order to evaluate which diet most closely mirrors human obesity.Methods:Wistar rats were fed four different diets (WD, CAF, HFD and a low-fat diet) for 18 weeks. Metabolic parameters and gut microbiota changes were then characterized.Results:Rats fed the four different diets exhibited completely different phenotypes, highlighting the importance of diet selection. This study also revealed that WD most effectively induced obesity and obesity-related disorders, and thus proved to be a robust model of human obesity. Moreover, WD-fed rats developed obesity and obesity-related comorbidities independent of major alterations in gut microbiota composition (dysbiosis), whereas CAF-fed rats developed the greatest dysbiosis independent of obesity. We also characterized gut microbiota after feeding on these four different diets and identified five genera that might be involved in the pathogenesis of obesity.Conclusions:These data suggest that diet, and not the obese state, was the major driving force behind gut microbiota changes. Moreover, the marked dysbiosis observed in CAF-fed rats might have resulted from the presence of several additives present in the CAF diet, or even a lack of essential vitamins and minerals. Based on our findings, we recommend the use of the prototypic WD (designed here) in DIO models. Conversely, CAF could be used to investigate the effects of excessive consumption of industrially produced and highly processed foods, which are characteristic of Western society.Antecedentes / Objetivos: La obesidad es un trastorno metabólico que predispone a los pacientes a numerosas enfermedades y se ha convertido en un importante problema de salud pública mundial. Los modelos animales de la obesidad inducida por la dieta (DIO) se utilizan con frecuencia para estudiar la obesidad, pero el modelo DIO que refleja con mayor precisión la patología de la obesidad humana aún no está claro. En este estudio, diseñamos una dieta basada en la dieta occidental humana (WD) y la comparamos con la dieta de cafetería (CAF) y la dieta alta en grasas (HFD) para evaluar qué dieta refleja mejor la obesidad humana. Métodos: Wistar las ratas fueron alimentadas con cuatro dietas diferentes (WD, CAF, HFD y una dieta baja en grasa) durante 18 semanas. Luego se caracterizaron los parámetros metabólicos y los cambios en la microbiota intestinal. Resultados: Las ratas alimentadas con las cuatro dietas diferentes mostraron fenotipos completamente diferentes, destacando la importancia de la selección de la dieta. Este estudio también reveló que la WD inducía con mayor eficacia la obesidad y los trastornos relacionados con la obesidad, y por lo tanto demostró ser un modelo robusto de la obesidad humana. Además, las ratas alimentadas con WD desarrollaron obesidad y comorbilidades relacionadas con la obesidad independientes de alteraciones importantes en la composición de la microbiota intestinal (disbiosis), mientras que las ratas alimentadas con CAF desarrollaron la mayor disbiosis independiente de la obesidad. También caracterizamos la microbiota intestinal después de alimentarnos con estas cuatro dietas diferentes e identificamos cinco géneros que podrían estar involucrados en la patogénesis de la obesidad. Conclusiones: Estos datos sugieren que la dieta, y no el estado obeso, fue la principal fuerza impulsora detrás de los cambios en la microbiota intestinal. Además, la marcada disbiosis observada en ratas alimentadas con CAF podría haber resultado de la presencia de varios aditivos presentes en la dieta CAF, o incluso la falta de vitaminas y minerales esenciales. Sobre la base de nuestros hallazgos, recomendamos el uso del prototipo WD (diseñado aquí) en modelos DIO. A la inversa, CAF podría utilizarse para investigar los efectos del consumo excesivo de alimentos producidos industrialmente y altamente procesados, que son característicos de la sociedad occidental.Calixto Bortolin, Rafael-0000-0003-4780-8499-600Rodrigues Vargas, Amanda-13930865-2282-40d5-aa08-3ac5cd827b9c-0Gasparotto, Juciano-a7ba2163-3c88-45e4-b4f0-627833f8b03f-0Rodrigues Chaves, Paloma-13b466fc-a666-4b1b-a6ff-d536aefe3c82-0Schnorr, Carlos Eduardo-0000-0002-2047-2107-600Da Boit Martinello, Katia-332fc0f5-2d21-40f8-b210-63ad92c93b2a-0Kleber Silveira, Alexandre-15e11da5-dc14-47cd-9313-df43047bde99-0Rabelo, Thallita Kelly-3cca46bc-d2d5-4941-8fff-bd3b0074486b-0Gelain, D. P-410bf449-252e-437c-9afc-71a1866db0eb-0Moreira Fonseca, Jose Claudio-bf822091-a59e-4990-8884-64ed361dbd5c-0engUniversidad de la CostaAttribution-NonCommercial-ShareAlike 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Animal experimentAnimal modelArticleCafeteria dietComorbidityComparative studyControlled studyDietDiet induced obesityDysbiosisFood preferenceIntestine floraLipid dietLow fat dietMaleMetabolic parametersNonhumanPhenotypePriority journalRatWestern dietExperimento animalModelo animalArtículoDieta de la cafeteriaComorbilidadEstudio comparativoEstudio controladoDietaDieta inducida por la obesidadDisbiosisPreferencia de alimentosFlora intestinalDieta lipídicaDieta baja en grasasMasculinoParámetros metabolicosNo humanoFenotipoRevista de prioridadRataDieta occidentalA new animal diet based on human western diet is a robust diet-induced obesity model: comparison to high-fat and cafeteria diets in term of metabolic and gut microbiota disruptionUna nueva dieta animal basada en la dieta occidental humana es un modelo robusto de obesidad inducida por la dieta: comparación con las dietas ricas en grasas y en la cafetería en términos de alteración metabólica y microbiota intestinalArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/acceptedVersionPublicationORIGINALA New Animal Diet Based On Human.pdfA New Animal Diet Based On Human.pdfapplication/pdf183476https://repositorio.cuc.edu.co/bitstreams/47e79436-2b13-4a35-a63c-f6929ff056b9/downloadb71ec563fb56860fa9a8620d5930ce35MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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