Melanopsin-Mediated Transduction in Unconventional photoreceptors.
El proyecto permitió establecer por primera vez que en retina de pollo una población de células de la capa nuclear interna poseen fotosensibilidad intrínseca. Este organismo se plantea entonces como un modelo sumamente atractivo no solo para esclarecer los mecanismos de señalización de la melanopsin...
- Autores:
-
Gomez, María del Pilar
- Tipo de recurso:
- Investigation report
- Fecha de publicación:
- 2014
- Institución:
- Minciencias
- Repositorio:
- Repositorio Minciencias
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.minciencias.gov.co:20.500.14143/39574
- Acceso en línea:
- https://colciencias.metadirectorio.org/handle/11146/39574
http://colciencias.metabiblioteca.com.co
- Palabra clave:
- Percepción visual
Melanopsina
Pigmento visual
Células ganglionares de la retina
Fototransducción
Señalización
Visión
Patch Clamp
- Rights
- openAccess
- License
- http://purl.org/coar/access_right/c_abf2
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Melanopsin-Mediated Transduction in Unconventional photoreceptors. |
| title |
Melanopsin-Mediated Transduction in Unconventional photoreceptors. |
| spellingShingle |
Melanopsin-Mediated Transduction in Unconventional photoreceptors. Percepción visual Melanopsina Pigmento visual Células ganglionares de la retina Fototransducción Señalización Visión Patch Clamp |
| title_short |
Melanopsin-Mediated Transduction in Unconventional photoreceptors. |
| title_full |
Melanopsin-Mediated Transduction in Unconventional photoreceptors. |
| title_fullStr |
Melanopsin-Mediated Transduction in Unconventional photoreceptors. |
| title_full_unstemmed |
Melanopsin-Mediated Transduction in Unconventional photoreceptors. |
| title_sort |
Melanopsin-Mediated Transduction in Unconventional photoreceptors. |
| dc.creator.fl_str_mv |
Gomez, María del Pilar |
| dc.contributor.author.none.fl_str_mv |
Gomez, María del Pilar |
| dc.contributor.corporatename.spa.fl_str_mv |
Universidad Nacional de Colombia Centro Internacional de Física (CIF) (Colombia) |
| dc.contributor.researchgroup.none.fl_str_mv |
COL0022691 - Biofisica y Biologia de Membranas COL0063760 - Biofísica de la Señalización Celular COL0086143 - Neurofisiologia Celular |
| dc.subject.armarc.none.fl_str_mv |
Percepción visual |
| topic |
Percepción visual Melanopsina Pigmento visual Células ganglionares de la retina Fototransducción Señalización Visión Patch Clamp |
| dc.subject.proposal.spa.fl_str_mv |
Melanopsina Pigmento visual Células ganglionares de la retina Fototransducción Señalización Visión |
| dc.subject.proposal.en.fl_str_mv |
Patch Clamp |
| description |
El proyecto permitió establecer por primera vez que en retina de pollo una población de células de la capa nuclear interna poseen fotosensibilidad intrínseca. Este organismo se plantea entonces como un modelo sumamente atractivo no solo para esclarecer los mecanismos de señalización de la melanopsina sino para aportar nuevos elementos a la historia evolutiva de la fototransducción. (Apartes del texto). |
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2014 |
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2014-04 |
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2019-10-30T15:36:18Z 2020-12-18T00:36:15Z |
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2019-10-30T15:36:18Z 2020-12-18T00:36:15Z |
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Informe de investigación |
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http://purl.org/coar/resource_type/c_93fc |
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Text |
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info:eu-repo/semantics/report |
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https://purl.org/redcol/resource_type/PID |
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https://colciencias.metadirectorio.org/handle/11146/39574 |
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Colciencias |
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Repositorio Colciencias |
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http://colciencias.metabiblioteca.com.co |
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https://colciencias.metadirectorio.org/handle/11146/39574 http://colciencias.metabiblioteca.com.co |
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spa |
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Informe; |
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info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 |
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https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
| dc.format.extent.spa.fl_str_mv |
23 páginas. |
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Colombia |
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2012-2014 |
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Gomez, María del Pilar41f923ab0c0fba5353c418fb206f36c8-1Universidad Nacional de ColombiaCentro Internacional de Física (CIF) (Colombia)COL0022691 - Biofisica y Biologia de MembranasCOL0063760 - Biofísica de la Señalización CelularCOL0086143 - Neurofisiologia CelularColombia2012-20142019-10-30T15:36:18Z2020-12-18T00:36:15Z2019-10-30T15:36:18Z2020-12-18T00:36:15Z2014-04https://colciencias.metadirectorio.org/handle/11146/39574ColcienciasRepositorio Colcienciashttp://colciencias.metabiblioteca.com.coEl proyecto permitió establecer por primera vez que en retina de pollo una población de células de la capa nuclear interna poseen fotosensibilidad intrínseca. Este organismo se plantea entonces como un modelo sumamente atractivo no solo para esclarecer los mecanismos de señalización de la melanopsina sino para aportar nuevos elementos a la historia evolutiva de la fototransducción. (Apartes del texto).23 páginas.spaInforme;Melanopsin-Mediated Transduction in Unconventional photoreceptors.Informe de investigaciónhttp://purl.org/coar/resource_type/c_18wshttp://purl.org/coar/resource_type/c_93fcTextinfo:eu-repo/semantics/reporthttps://purl.org/redcol/resource_type/PIDinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/version/c_71e4c1898caa6e32info:eu-repo/semantics/submittedVersioninfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by/4.0/Percepción visualMelanopsinaPigmento visualCélulas ganglionares de la retinaFototransducciónSeñalizaciónVisiónPatch ClampEstudiantes, Profesores, Comunidad científica colombiana, etc.222852128276443-2011Departamento Administrativo de Ciencia, Tecnología e Innovación [CO] ColcienciasGeneral Objectives : The long-term goals of this project are to elucidate the cellular and molecular underpinnings of light-sensing in native cells that utilize the photopigment melanopsin. The chicken retina presents itself as an interesting and valuable system to study melanopsin transduction due to the abundant expression of this protein in the inner nuclear layer possibly in bipolar and horizontal cells. The functional role of melanopsin in these retinal layer is completely unknown which opens the possibility of uncovering a completely novel light sensor. More important this organism may provide clues on the evolution of different lineages of photoreceptors and their respective signaling cascades.Specific Objectives : 1. Develop a preparation of functionally viable cells from the retina of chicken embryos, isolated by means of enzymatic treatment and mechanical dispersion.Specific Objectives : 2. Determine which of the defined cell types present in the inner nuclear layer of chicken embryo retinas express melanopsin.Specific Objectives : 3. Use a fluorescence assay to establish the presence of retinal in melanosin-expressing cells, which would suggest that melanosin forms a functional photopigment.Specific Objectives : 4. Utilize patch-clamp recordings to examine whether melanopsin-expressing cells are intrinsically photosensitive.Specific Objectives : 5. Detect and localize key signaling molecules of the phosphoinositide pathway in the chicken retina, using Western blot analysis, and isolated-cell immunocytochemistry.Specific Objectives : 6. Ascertain the functional role of key downstream elements of the cascade, by means of single-cell electrophysiology in conjunction with Ca fluorescence measurements and pharmacology.PublicationORIGINAL222852128276 20142430063072.pdf222852128276 20142430063072.pdfInforme finalapplication/pdf8838701https://repositorio.minciencias.gov.co/bitstreams/6f038cf4-fa7d-4d2c-9f3b-415a0d21d272/downloade5ccfd10d31a951e5fb7a42b643931eaMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-814798https://repositorio.minciencias.gov.co/bitstreams/b28edbe9-7909-4dbb-90d9-641f953ad072/download88794144ff048353b359a3174871b0d5MD52license.txtlicense.txttext/plain; charset=utf-80https://repositorio.minciencias.gov.co/bitstreams/465e0e30-5f47-4f92-a2db-1128243ff019/downloadd41d8cd98f00b204e9800998ecf8427eMD55TEXT222852128276 20142430063072.pdf.txt222852128276 20142430063072.pdf.txtExtracted texttext/plain23https://repositorio.minciencias.gov.co/bitstreams/508fc203-bfa1-4e18-889d-afd6bed7ab54/download392b92377d3166969ac79cc37a0ba5fbMD53THUMBNAIL222852128276 20142430063072.pdf.jpg222852128276 20142430063072.pdf.jpgGenerated Thumbnailimage/jpeg6554https://repositorio.minciencias.gov.co/bitstreams/b67175f7-3454-4553-969c-9210a864d453/downloadc6858bffaf9ecd3d3a2ad7924391a3fbMD5420.500.14143/39574oai:repositorio.minciencias.gov.co:20.500.14143/395742023-11-29 17:32:21.259restrictedhttps://repositorio.minciencias.gov.coRepositorio Institucional de Mincienciascendoc@minciencias.gov.co |