Temperatura de almacenamiento y cantidad de ARN extraído de plasma maternos
The discovery of nucleic acids in maternal plasma unlocked new possibilities for noninvasive prenatal diagnosis. However, the factors affecting the concentration of fetal RNA in maternal plasma samples are unspecified. Here we studied the effect of storage time (15 to 30 days) and temperature (4°C,...
- Autores:
- Tipo de recurso:
- article
- Fecha de publicación:
- 2013
- Institución:
- Pontificia Universidad Javeriana
- Repositorio:
- Repositorio Universidad Javeriana
- Idioma:
- eng
- OAI Identifier:
- oai:repository.javeriana.edu.co:10554/31751
- Acceso en línea:
- http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4671
http://hdl.handle.net/10554/31751
- Palabra clave:
- null
Ribonucleic acid; plasma; pregnancy complications
null
Health Sciences
Ribonucleic acid; plasma; pregnancy complications
241007
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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Temperatura de almacenamiento y cantidad de ARN extraído de plasma maternosTemperature of storage and amount of RNA extracted from maternal plasmaAyala Ramírez, Paola Andrea; Pontificia Universidad JaverianaGonzalez Ropero, Liz Ariane; Pontificia Universidad JaverianaGarcía Robles, Reggie; Universidad El BosquenullRibonucleic acid; plasma; pregnancy complicationsnullHealth SciencesRibonucleic acid; plasma; pregnancy complications241007The discovery of nucleic acids in maternal plasma unlocked new possibilities for noninvasive prenatal diagnosis. However, the factors affecting the concentration of fetal RNA in maternal plasma samples are unspecified. Here we studied the effect of storage time (15 to 30 days) and temperature (4°C, -20°C and ambient) and of the presence of Trizol on the concentration of RNA in plasma samples taken from pregnant women before their 20th week. Thirty-three RNA samples were extracted from plasma and analyzed by spectrophotometry. The amount of RNA was statistically lower in samples stored at -20°C whereas storage time and the presence or absence of Trizol did not affect RNA level. Temperature affected the concentration of RNA, rather than time and the addition of Trizol. In conclusion, temperature is akey factor in the extraction of RNA, and the freeze-thaw process most likely affects RNA concentrations negatively.The discovery of nucleic acids in maternal plasma unlocked new possibilities for noninvasive prenatal diagnosis. However, the factors affecting the concentration of fetal RNA in maternal plasma samples are unspecified. Here we studied the effect of storage time (15 to 30 days) and temperature (4°C, -20°C and ambient) and of the presence of Trizol on the concentration of RNA in plasma samples taken from pregnant women before their 20th week. Thirty-three RNA samples were extracted from plasma and analyzed by spectrophotometry. The amount of RNA was statistically lower in samples stored at -20°C whereas storage time and the presence or absence of Trizol did not affect RNA level. Temperature affected the concentration of RNA, rather than time and the addition of Trizol. In conclusion, temperature is a key factor in the extraction of RNA, and the freeze-thaw process most likely affects RNA concentrations negatively.Pontificia Universidad Javeriananullnull2018-02-24T16:00:35Z2020-04-15T18:08:20Z2018-02-24T16:00:35Z2020-04-15T18:08:20Z2013-08-16http://purl.org/coar/version/c_970fb48d4fbd8a85Artículo de revistahttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPDFapplication/pdftext/htmlhttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/467110.11144/Javeriana.SC18-2.taca2027-13520122-7483http://hdl.handle.net/10554/31751enghttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/4671/4884http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4671/6503http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/4671/1157http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/4671/1158http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/4671/1159http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/4671/1184http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/4671/1185Universitas Scientiarum; Vol 18, No 2 (2013); 181-187Universitas Scientiarum; Vol 18, No 2 (2013); 181-187Universitas Scientiarum; Vol 18, No 2 (2013); 181-187nullnullnullnullnullnullAtribución-NoComercial-SinDerivadas 4.0 Internacionalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2reponame:Repositorio Universidad Javerianainstname:Pontificia Universidad Javerianainstacron:Pontificia Universidad Javeriana2023-03-28T21:16:00Z |
dc.title.none.fl_str_mv |
Temperatura de almacenamiento y cantidad de ARN extraído de plasma maternos Temperature of storage and amount of RNA extracted from maternal plasma |
title |
Temperatura de almacenamiento y cantidad de ARN extraído de plasma maternos |
spellingShingle |
Temperatura de almacenamiento y cantidad de ARN extraído de plasma maternos Ayala Ramírez, Paola Andrea; Pontificia Universidad Javeriana null Ribonucleic acid; plasma; pregnancy complications null Health Sciences Ribonucleic acid; plasma; pregnancy complications 241007 |
title_short |
Temperatura de almacenamiento y cantidad de ARN extraído de plasma maternos |
title_full |
Temperatura de almacenamiento y cantidad de ARN extraído de plasma maternos |
title_fullStr |
Temperatura de almacenamiento y cantidad de ARN extraído de plasma maternos |
title_full_unstemmed |
Temperatura de almacenamiento y cantidad de ARN extraído de plasma maternos |
title_sort |
Temperatura de almacenamiento y cantidad de ARN extraído de plasma maternos |
dc.creator.none.fl_str_mv |
Ayala Ramírez, Paola Andrea; Pontificia Universidad Javeriana Gonzalez Ropero, Liz Ariane; Pontificia Universidad Javeriana García Robles, Reggie; Universidad El Bosque |
author |
Ayala Ramírez, Paola Andrea; Pontificia Universidad Javeriana |
author_facet |
Ayala Ramírez, Paola Andrea; Pontificia Universidad Javeriana Gonzalez Ropero, Liz Ariane; Pontificia Universidad Javeriana García Robles, Reggie; Universidad El Bosque |
author_role |
author |
author2 |
Gonzalez Ropero, Liz Ariane; Pontificia Universidad Javeriana García Robles, Reggie; Universidad El Bosque |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
null null |
dc.subject.none.fl_str_mv |
null Ribonucleic acid; plasma; pregnancy complications null Health Sciences Ribonucleic acid; plasma; pregnancy complications 241007 |
topic |
null Ribonucleic acid; plasma; pregnancy complications null Health Sciences Ribonucleic acid; plasma; pregnancy complications 241007 |
description |
The discovery of nucleic acids in maternal plasma unlocked new possibilities for noninvasive prenatal diagnosis. However, the factors affecting the concentration of fetal RNA in maternal plasma samples are unspecified. Here we studied the effect of storage time (15 to 30 days) and temperature (4°C, -20°C and ambient) and of the presence of Trizol on the concentration of RNA in plasma samples taken from pregnant women before their 20th week. Thirty-three RNA samples were extracted from plasma and analyzed by spectrophotometry. The amount of RNA was statistically lower in samples stored at -20°C whereas storage time and the presence or absence of Trizol did not affect RNA level. Temperature affected the concentration of RNA, rather than time and the addition of Trizol. In conclusion, temperature is akey factor in the extraction of RNA, and the freeze-thaw process most likely affects RNA concentrations negatively. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-08-16 2018-02-24T16:00:35Z 2018-02-24T16:00:35Z 2020-04-15T18:08:20Z 2020-04-15T18:08:20Z |
dc.type.none.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 Artículo de revista http://purl.org/coar/resource_type/c_6501 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4671 10.11144/Javeriana.SC18-2.taca 2027-1352 0122-7483 http://hdl.handle.net/10554/31751 |
url |
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4671 http://hdl.handle.net/10554/31751 |
identifier_str_mv |
10.11144/Javeriana.SC18-2.taca 2027-1352 0122-7483 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4671/4884 http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4671/6503 http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/4671/1157 http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/4671/1158 http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/4671/1159 http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/4671/1184 http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/4671/1185 Universitas Scientiarum; Vol 18, No 2 (2013); 181-187 Universitas Scientiarum; Vol 18, No 2 (2013); 181-187 Universitas Scientiarum; Vol 18, No 2 (2013); 181-187 |
dc.rights.none.fl_str_mv |
Atribución-NoComercial-SinDerivadas 4.0 Internacional info:eu-repo/semantics/openAccess http://purl.org/coar/access_right/c_abf2 |
rights_invalid_str_mv |
Atribución-NoComercial-SinDerivadas 4.0 Internacional http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
PDF application/pdf text/html |
dc.coverage.none.fl_str_mv |
null null null null null null |
dc.publisher.none.fl_str_mv |
Pontificia Universidad Javeriana |
publisher.none.fl_str_mv |
Pontificia Universidad Javeriana |
dc.source.none.fl_str_mv |
reponame:Repositorio Universidad Javeriana instname:Pontificia Universidad Javeriana instacron:Pontificia Universidad Javeriana |
instname_str |
Pontificia Universidad Javeriana |
instacron_str |
Pontificia Universidad Javeriana |
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Pontificia Universidad Javeriana |
reponame_str |
Repositorio Universidad Javeriana |
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Repositorio Universidad Javeriana |
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