Temperatura de almacenamiento y cantidad de ARN extraído de plasma maternos

The discovery of nucleic acids in maternal plasma unlocked new possibilities for noninvasive prenatal diagnosis. However, the factors affecting the concentration of fetal RNA in maternal plasma samples are unspecified. Here we studied the effect of storage time (15 to 30 days) and temperature (4°C,...

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Tipo de recurso:
article
Fecha de publicación:
2013
Institución:
Pontificia Universidad Javeriana
Repositorio:
Repositorio Universidad Javeriana
Idioma:
eng
OAI Identifier:
oai:repository.javeriana.edu.co:10554/31751
Acceso en línea:
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4671
http://hdl.handle.net/10554/31751
Palabra clave:
null
Ribonucleic acid; plasma; pregnancy complications
null
Health Sciences
Ribonucleic acid; plasma; pregnancy complications
241007
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openAccess
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Atribución-NoComercial-SinDerivadas 4.0 Internacional
id JAVERIANA_ff598396e7f500496ea1eb91aebadcbb
oai_identifier_str oai:repository.javeriana.edu.co:10554/31751
network_acronym_str JAVERIANA
network_name_str Repositorio Universidad Javeriana
repository_id_str
spelling Temperatura de almacenamiento y cantidad de ARN extraído de plasma maternosTemperature of storage and amount of RNA extracted from maternal plasmaAyala Ramírez, Paola Andrea; Pontificia Universidad JaverianaGonzalez Ropero, Liz Ariane; Pontificia Universidad JaverianaGarcía Robles, Reggie; Universidad El BosquenullRibonucleic acid; plasma; pregnancy complicationsnullHealth SciencesRibonucleic acid; plasma; pregnancy complications241007The discovery of nucleic acids in maternal plasma unlocked new possibilities for noninvasive prenatal diagnosis. However, the factors affecting the concentration of fetal RNA in maternal plasma samples are unspecified. Here we studied the effect of storage time (15 to 30 days) and temperature (4°C, -20°C and ambient) and of the presence of Trizol on the concentration of RNA in plasma samples taken from pregnant women before their 20th week. Thirty-three RNA samples were extracted from plasma and analyzed by spectrophotometry. The amount of RNA was statistically lower in samples stored at -20°C whereas storage time and the presence or absence of Trizol did not affect RNA level. Temperature affected the concentration of RNA, rather than time and the addition of Trizol. In conclusion, temperature is akey factor in the extraction of RNA, and the freeze-thaw process most likely affects RNA concentrations negatively.The discovery of nucleic acids in maternal plasma unlocked new possibilities for noninvasive prenatal diagnosis. However, the factors affecting the concentration of fetal RNA in maternal plasma samples are unspecified. Here we studied the effect of storage time (15 to 30 days) and temperature (4°C, -20°C and ambient) and of the presence of Trizol on the concentration of RNA in plasma samples taken from pregnant women before their 20th week. Thirty-three RNA samples were extracted from plasma and analyzed by spectrophotometry. The amount of RNA was statistically lower in samples stored at -20°C whereas storage time and the presence or absence of Trizol did not affect RNA level. Temperature affected the concentration of RNA, rather than time and the addition of Trizol. In conclusion, temperature is a key factor in the extraction of RNA, and the freeze-thaw process most likely affects RNA concentrations  negatively.Pontificia Universidad Javeriananullnull2018-02-24T16:00:35Z2020-04-15T18:08:20Z2018-02-24T16:00:35Z2020-04-15T18:08:20Z2013-08-16http://purl.org/coar/version/c_970fb48d4fbd8a85Artículo de revistahttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPDFapplication/pdftext/htmlhttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/467110.11144/Javeriana.SC18-2.taca2027-13520122-7483http://hdl.handle.net/10554/31751enghttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/4671/4884http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4671/6503http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/4671/1157http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/4671/1158http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/4671/1159http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/4671/1184http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/4671/1185Universitas Scientiarum; Vol 18, No 2 (2013); 181-187Universitas Scientiarum; Vol 18, No 2 (2013); 181-187Universitas Scientiarum; Vol 18, No 2 (2013); 181-187nullnullnullnullnullnullAtribución-NoComercial-SinDerivadas 4.0 Internacionalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2reponame:Repositorio Universidad Javerianainstname:Pontificia Universidad Javerianainstacron:Pontificia Universidad Javeriana2023-03-28T21:16:00Z
dc.title.none.fl_str_mv Temperatura de almacenamiento y cantidad de ARN extraído de plasma maternos
Temperature of storage and amount of RNA extracted from maternal plasma
title Temperatura de almacenamiento y cantidad de ARN extraído de plasma maternos
spellingShingle Temperatura de almacenamiento y cantidad de ARN extraído de plasma maternos
Ayala Ramírez, Paola Andrea; Pontificia Universidad Javeriana
null
Ribonucleic acid; plasma; pregnancy complications
null
Health Sciences
Ribonucleic acid; plasma; pregnancy complications
241007
title_short Temperatura de almacenamiento y cantidad de ARN extraído de plasma maternos
title_full Temperatura de almacenamiento y cantidad de ARN extraído de plasma maternos
title_fullStr Temperatura de almacenamiento y cantidad de ARN extraído de plasma maternos
title_full_unstemmed Temperatura de almacenamiento y cantidad de ARN extraído de plasma maternos
title_sort Temperatura de almacenamiento y cantidad de ARN extraído de plasma maternos
dc.creator.none.fl_str_mv Ayala Ramírez, Paola Andrea; Pontificia Universidad Javeriana
Gonzalez Ropero, Liz Ariane; Pontificia Universidad Javeriana
García Robles, Reggie; Universidad El Bosque
author Ayala Ramírez, Paola Andrea; Pontificia Universidad Javeriana
author_facet Ayala Ramírez, Paola Andrea; Pontificia Universidad Javeriana
Gonzalez Ropero, Liz Ariane; Pontificia Universidad Javeriana
García Robles, Reggie; Universidad El Bosque
author_role author
author2 Gonzalez Ropero, Liz Ariane; Pontificia Universidad Javeriana
García Robles, Reggie; Universidad El Bosque
author2_role author
author
dc.contributor.none.fl_str_mv null
null
dc.subject.none.fl_str_mv null
Ribonucleic acid; plasma; pregnancy complications
null
Health Sciences
Ribonucleic acid; plasma; pregnancy complications
241007
topic null
Ribonucleic acid; plasma; pregnancy complications
null
Health Sciences
Ribonucleic acid; plasma; pregnancy complications
241007
description The discovery of nucleic acids in maternal plasma unlocked new possibilities for noninvasive prenatal diagnosis. However, the factors affecting the concentration of fetal RNA in maternal plasma samples are unspecified. Here we studied the effect of storage time (15 to 30 days) and temperature (4°C, -20°C and ambient) and of the presence of Trizol on the concentration of RNA in plasma samples taken from pregnant women before their 20th week. Thirty-three RNA samples were extracted from plasma and analyzed by spectrophotometry. The amount of RNA was statistically lower in samples stored at -20°C whereas storage time and the presence or absence of Trizol did not affect RNA level. Temperature affected the concentration of RNA, rather than time and the addition of Trizol. In conclusion, temperature is akey factor in the extraction of RNA, and the freeze-thaw process most likely affects RNA concentrations negatively.
publishDate 2013
dc.date.none.fl_str_mv 2013-08-16
2018-02-24T16:00:35Z
2018-02-24T16:00:35Z
2020-04-15T18:08:20Z
2020-04-15T18:08:20Z
dc.type.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
Artículo de revista
http://purl.org/coar/resource_type/c_6501
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4671
10.11144/Javeriana.SC18-2.taca
2027-1352
0122-7483
http://hdl.handle.net/10554/31751
url http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4671
http://hdl.handle.net/10554/31751
identifier_str_mv 10.11144/Javeriana.SC18-2.taca
2027-1352
0122-7483
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4671/4884
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4671/6503
http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/4671/1157
http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/4671/1158
http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/4671/1159
http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/4671/1184
http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/4671/1185
Universitas Scientiarum; Vol 18, No 2 (2013); 181-187
Universitas Scientiarum; Vol 18, No 2 (2013); 181-187
Universitas Scientiarum; Vol 18, No 2 (2013); 181-187
dc.rights.none.fl_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
info:eu-repo/semantics/openAccess
http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv PDF
application/pdf
text/html
dc.coverage.none.fl_str_mv null
null
null
null
null
null
dc.publisher.none.fl_str_mv Pontificia Universidad Javeriana
publisher.none.fl_str_mv Pontificia Universidad Javeriana
dc.source.none.fl_str_mv reponame:Repositorio Universidad Javeriana
instname:Pontificia Universidad Javeriana
instacron:Pontificia Universidad Javeriana
instname_str Pontificia Universidad Javeriana
instacron_str Pontificia Universidad Javeriana
institution Pontificia Universidad Javeriana
reponame_str Repositorio Universidad Javeriana
collection Repositorio Universidad Javeriana
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