NARINGENIN ENHANCED EFFICIENCY OF GUS ACTIVITY IN Passiflora mollissima (H.B.K.) Bailey

The flavonoid naringenin has been investigated as a possible vir gene inducer in Agrobacterium mediated transformation in Passiflora mollissima, P. giberti and Nicotiana tabacum cv. Xanthi. Thetransformation efficiency percentage of explants showing blue GUS expression and the extent of staining fol...

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Tipo de recurso:
article
Fecha de publicación:
2004
Institución:
Pontificia Universidad Javeriana
Repositorio:
Repositorio Universidad Javeriana
Idioma:
eng
OAI Identifier:
oai:repository.javeriana.edu.co:10554/31943
Acceso en línea:
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4922
http://hdl.handle.net/10554/31943
Palabra clave:
null
naringenin; Agrobacterium; acetosyringone; Passifloras
null
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openAccess
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Atribución-NoComercial-SinDerivadas 4.0 Internacional
id JAVERIANA_a4271d1bbe9742fc54138df364fb51cf
oai_identifier_str oai:repository.javeriana.edu.co:10554/31943
network_acronym_str JAVERIANA
network_name_str Repositorio Universidad Javeriana
repository_id_str
spelling NARINGENIN ENHANCED EFFICIENCY OF GUS ACTIVITY IN Passiflora mollissima (H.B.K.) BaileyCancino, G. O.; Departamento de Biología Facultad de Ciencias Pontificia Universidad Javeriana, BogotáGill, M. I.S.; Department of Genetic and Biotechnology, P.A.U., Ludhiana, IndiaDavey, M. R.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UKPower, J. B.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UKLowe, K. C.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UKnullnaringenin; Agrobacterium; acetosyringone; PassiflorasnullThe flavonoid naringenin has been investigated as a possible vir gene inducer in Agrobacterium mediated transformation in Passiflora mollissima, P. giberti and Nicotiana tabacum cv. Xanthi. Thetransformation efficiency percentage of explants showing blue GUS expression and the extent of staining following inoculation with Agrobacterium tumefaciens strains EHA 105 and 1065, carrying gus and nptII genes was enhanced with the supplementation of the co-cultivation medium with naringenin. Supplementation of medium with 100mM (strain EHA 105) and 300 mM (strain 1065) naringenin was most effective at enhancing mean (±s.e.m., n=3) GUS activity in leaf explants (20.3± 2.4%, strain EHA; 105; 6.0 ± 0.57%, strain 1065) and nodal segments (16.7 ± 2.4% strain EHA 105; 8.3 ± 0.57% strain 1065) of P. mollissima. In P. giberti and N. tabacum maximum GUS activitywas obtained in leaf and root explants with 100mM naringenin for both strains analysed. Additionally, when naringenin was added to Luria Bertani (LB) medium, both bacterial growth via optical density and colony forming units were higher when compared to control. This is the first report of the use ofnaringenin to enhance gene transfer from Agrobacterium to plants. These findings suggest that naringenin can be used as an alternative to acetosyringone for vir gene induction in Agrobacterium. This approachmay be especially useful in plants that are generally recalcitrant to Agrobacterium-mediated transformation.Pontificia Universidad Javeriananull2018-02-24T16:01:01Z2020-04-15T18:08:45Z2018-02-24T16:01:01Z2020-04-15T18:08:45Z2004-01-10http://purl.org/coar/version/c_970fb48d4fbd8a85Artículo de revistahttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPDFapplication/pdfhttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/49222027-13520122-7483http://hdl.handle.net/10554/31943enghttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/4922/3797Universitas Scientiarum; Vol 9, No 1 (2004); 47-57Universitas Scientiarum; Vol 9, No 1 (2004); 47-57Universitas Scientiarum; Vol 9, No 1 (2004); 47-57nullnullnullAtribución-NoComercial-SinDerivadas 4.0 Internacionalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2reponame:Repositorio Universidad Javerianainstname:Pontificia Universidad Javerianainstacron:Pontificia Universidad Javeriana2023-03-28T21:15:56Z
dc.title.none.fl_str_mv NARINGENIN ENHANCED EFFICIENCY OF GUS ACTIVITY IN Passiflora mollissima (H.B.K.) Bailey
title NARINGENIN ENHANCED EFFICIENCY OF GUS ACTIVITY IN Passiflora mollissima (H.B.K.) Bailey
spellingShingle NARINGENIN ENHANCED EFFICIENCY OF GUS ACTIVITY IN Passiflora mollissima (H.B.K.) Bailey
Cancino, G. O.; Departamento de Biología Facultad de Ciencias Pontificia Universidad Javeriana, Bogotá
null
naringenin; Agrobacterium; acetosyringone; Passifloras
null
title_short NARINGENIN ENHANCED EFFICIENCY OF GUS ACTIVITY IN Passiflora mollissima (H.B.K.) Bailey
title_full NARINGENIN ENHANCED EFFICIENCY OF GUS ACTIVITY IN Passiflora mollissima (H.B.K.) Bailey
title_fullStr NARINGENIN ENHANCED EFFICIENCY OF GUS ACTIVITY IN Passiflora mollissima (H.B.K.) Bailey
title_full_unstemmed NARINGENIN ENHANCED EFFICIENCY OF GUS ACTIVITY IN Passiflora mollissima (H.B.K.) Bailey
title_sort NARINGENIN ENHANCED EFFICIENCY OF GUS ACTIVITY IN Passiflora mollissima (H.B.K.) Bailey
dc.creator.none.fl_str_mv Cancino, G. O.; Departamento de Biología Facultad de Ciencias Pontificia Universidad Javeriana, Bogotá
Gill, M. I.S.; Department of Genetic and Biotechnology, P.A.U., Ludhiana, India
Davey, M. R.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK
Power, J. B.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK
Lowe, K. C.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK
author Cancino, G. O.; Departamento de Biología Facultad de Ciencias Pontificia Universidad Javeriana, Bogotá
author_facet Cancino, G. O.; Departamento de Biología Facultad de Ciencias Pontificia Universidad Javeriana, Bogotá
Gill, M. I.S.; Department of Genetic and Biotechnology, P.A.U., Ludhiana, India
Davey, M. R.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK
Power, J. B.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK
Lowe, K. C.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK
author_role author
author2 Gill, M. I.S.; Department of Genetic and Biotechnology, P.A.U., Ludhiana, India
Davey, M. R.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK
Power, J. B.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK
Lowe, K. C.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK
author2_role author
author
author
author
dc.contributor.none.fl_str_mv null
dc.subject.none.fl_str_mv null
naringenin; Agrobacterium; acetosyringone; Passifloras
null
topic null
naringenin; Agrobacterium; acetosyringone; Passifloras
null
description The flavonoid naringenin has been investigated as a possible vir gene inducer in Agrobacterium mediated transformation in Passiflora mollissima, P. giberti and Nicotiana tabacum cv. Xanthi. Thetransformation efficiency percentage of explants showing blue GUS expression and the extent of staining following inoculation with Agrobacterium tumefaciens strains EHA 105 and 1065, carrying gus and nptII genes was enhanced with the supplementation of the co-cultivation medium with naringenin. Supplementation of medium with 100mM (strain EHA 105) and 300 mM (strain 1065) naringenin was most effective at enhancing mean (±s.e.m., n=3) GUS activity in leaf explants (20.3± 2.4%, strain EHA; 105; 6.0 ± 0.57%, strain 1065) and nodal segments (16.7 ± 2.4% strain EHA 105; 8.3 ± 0.57% strain 1065) of P. mollissima. In P. giberti and N. tabacum maximum GUS activitywas obtained in leaf and root explants with 100mM naringenin for both strains analysed. Additionally, when naringenin was added to Luria Bertani (LB) medium, both bacterial growth via optical density and colony forming units were higher when compared to control. This is the first report of the use ofnaringenin to enhance gene transfer from Agrobacterium to plants. These findings suggest that naringenin can be used as an alternative to acetosyringone for vir gene induction in Agrobacterium. This approachmay be especially useful in plants that are generally recalcitrant to Agrobacterium-mediated transformation.
publishDate 2004
dc.date.none.fl_str_mv 2004-01-10
2018-02-24T16:01:01Z
2018-02-24T16:01:01Z
2020-04-15T18:08:45Z
2020-04-15T18:08:45Z
dc.type.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
Artículo de revista
http://purl.org/coar/resource_type/c_6501
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4922
2027-1352
0122-7483
http://hdl.handle.net/10554/31943
url http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4922
http://hdl.handle.net/10554/31943
identifier_str_mv 2027-1352
0122-7483
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4922/3797
Universitas Scientiarum; Vol 9, No 1 (2004); 47-57
Universitas Scientiarum; Vol 9, No 1 (2004); 47-57
Universitas Scientiarum; Vol 9, No 1 (2004); 47-57
dc.rights.none.fl_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
info:eu-repo/semantics/openAccess
http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv PDF
application/pdf
dc.coverage.none.fl_str_mv null
null
null
dc.publisher.none.fl_str_mv Pontificia Universidad Javeriana
publisher.none.fl_str_mv Pontificia Universidad Javeriana
dc.source.none.fl_str_mv reponame:Repositorio Universidad Javeriana
instname:Pontificia Universidad Javeriana
instacron:Pontificia Universidad Javeriana
instname_str Pontificia Universidad Javeriana
instacron_str Pontificia Universidad Javeriana
institution Pontificia Universidad Javeriana
reponame_str Repositorio Universidad Javeriana
collection Repositorio Universidad Javeriana
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