CARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁ
Using an ELISA test, camation mottle virus (CarMV) was found to infect 82% of the camation samples collected from the Sabana of Bogotá. The isolated virus was mechanically inoculated on virus-free carnation plants and purified by sucrose gradient centrifugation. CarMV coat protein was analized by SD...
- Autores:
- Tipo de recurso:
- article
- Fecha de publicación:
- 2013
- Institución:
- Pontificia Universidad Javeriana
- Repositorio:
- Repositorio Universidad Javeriana
- Idioma:
- eng
- OAI Identifier:
- oai:repository.javeriana.edu.co:10554/31014
- Acceso en línea:
- http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4821
http://hdl.handle.net/10554/31014
- Palabra clave:
- null
cápside viral, carmovirus, clavel, dsRNA, electroforesis.
null
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
| Summary: | Using an ELISA test, camation mottle virus (CarMV) was found to infect 82% of the camation samples collected from the Sabana of Bogotá. The isolated virus was mechanically inoculated on virus-free carnation plants and purified by sucrose gradient centrifugation. CarMV coat protein was analized by SDS-PAGE showing a single component of about 38 Kda. Double-stranded RNA (dsRNA) extracted from carnation infected tissue showed 3 to 5 electrophoretic components ranging between 8.0 and 0.9 Kbp. Virus particles contained a genomic RNA of about 4.0 Kbp as revealed by agarosa gel electrophoresis. It was posible to distinguished 2 groups of electrophoretic pattems using RNAse T1 digestion of the RNA contained in the CarMV purified particles. |
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