Entrapment of Rhizobium sp. by fluidized bed technique using polymers as coating materials
The spray-drying technique was applied for the development of three solid formulations of Rhizobium. Sodium alginate and hydroxypropyl methylcellulose (HPMC) with concentrations of 0.5 % were used as polymers. Results showed that none of the solid formulations had negative effects in vitro on the gr...
- Autores:
- Tipo de recurso:
- article
- Fecha de publicación:
- 2016
- Institución:
- Pontificia Universidad Javeriana
- Repositorio:
- Repositorio Universidad Javeriana
- Idioma:
- eng
- OAI Identifier:
- oai:repository.javeriana.edu.co:10554/31339
- Acceso en línea:
- http://revistas.javeriana.edu.co/index.php/scientarium/article/view/13472
http://hdl.handle.net/10554/31339
- Palabra clave:
- Microbiologia Agricola
entrapment; spray-drying; coating, formulation.
Biotecnología Industrial
- Rights
- openAccess
- License
- Copyright (c) 2016 Universitas Scientiarum
| Summary: | The spray-drying technique was applied for the development of three solid formulations of Rhizobium. Sodium alginate and hydroxypropyl methylcellulose (HPMC) with concentrations of 0.5 % were used as polymers. Results showed that none of the solid formulations had negative effects in vitro on the growth-promoting capacities of Rhizobium sp. G58 (p < 0.05). PCA´s first three components explained 84.5 % of the total variance. This analysis concluded that the solid formulation had not negative effects on the biological nitrogen fixation activity in vitro or on the process of nodulation in greenhouse experiments. Symbiosis between Rhizobium and the plant was effective, which suggested that, under controlled conditions, the coating process with the polymers had allowed a controlled release of the bacteria and a proper transfer of Rhizobium sp. from the microparticles to the root of the plant. |
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