Bacteriome identified by next-generation sequencing in saliva, dental plaque, and tumor tissue of patients with oral squamous cell carcinoma
*
- Autores:
-
Erira, Alveiro
García Robayo, Dabeiba Adriana
Chalá, Andrés Ignacio
Moreno Torres, Andrei
Muñoz López, Eliana Elisa
Arregui, Angel Cid
Tobar, Fabian Tosse
Gamboa Jaimes, Fredy Omar
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2021
- Institución:
- Pontificia Universidad Javeriana
- Repositorio:
- Repositorio Universidad Javeriana
- Idioma:
- eng
- OAI Identifier:
- oai:repository.javeriana.edu.co:10554/59944
- Acceso en línea:
- https://www.openmicrobiologyjournal.com/VOLUME/15/PAGE/98/ABSTRACT/
http://hdl.handle.net/10554/59944
https://doi.org/10.2174/1874285802115010098
- Palabra clave:
- *
Microbiome
Eubiosi
Dysbiosi
Oral squamous cell carcinoma
Bacteria
Next-generation sequencing
- Rights
- License
- Atribución-NoComercial 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Bacteriome identified by next-generation sequencing in saliva, dental plaque, and tumor tissue of patients with oral squamous cell carcinoma |
| title |
Bacteriome identified by next-generation sequencing in saliva, dental plaque, and tumor tissue of patients with oral squamous cell carcinoma |
| spellingShingle |
Bacteriome identified by next-generation sequencing in saliva, dental plaque, and tumor tissue of patients with oral squamous cell carcinoma * Microbiome Eubiosi Dysbiosi Oral squamous cell carcinoma Bacteria Next-generation sequencing |
| title_short |
Bacteriome identified by next-generation sequencing in saliva, dental plaque, and tumor tissue of patients with oral squamous cell carcinoma |
| title_full |
Bacteriome identified by next-generation sequencing in saliva, dental plaque, and tumor tissue of patients with oral squamous cell carcinoma |
| title_fullStr |
Bacteriome identified by next-generation sequencing in saliva, dental plaque, and tumor tissue of patients with oral squamous cell carcinoma |
| title_full_unstemmed |
Bacteriome identified by next-generation sequencing in saliva, dental plaque, and tumor tissue of patients with oral squamous cell carcinoma |
| title_sort |
Bacteriome identified by next-generation sequencing in saliva, dental plaque, and tumor tissue of patients with oral squamous cell carcinoma |
| dc.creator.fl_str_mv |
Erira, Alveiro García Robayo, Dabeiba Adriana Chalá, Andrés Ignacio Moreno Torres, Andrei Muñoz López, Eliana Elisa Arregui, Angel Cid Tobar, Fabian Tosse Gamboa Jaimes, Fredy Omar |
| dc.contributor.author.none.fl_str_mv |
Erira, Alveiro García Robayo, Dabeiba Adriana Chalá, Andrés Ignacio Moreno Torres, Andrei Muñoz López, Eliana Elisa Arregui, Angel Cid Tobar, Fabian Tosse Gamboa Jaimes, Fredy Omar |
| dc.contributor.corporatename.spa.fl_str_mv |
Pontificia Universidad Javeriana. Facultad de Medicina. Departamento de Medicina Interna. Medicina Interna Pontificia Universidad Javeriana. Facultad de Medicina. Hospital Universitario San Ignacio |
| dc.subject.spa.fl_str_mv |
* |
| topic |
* Microbiome Eubiosi Dysbiosi Oral squamous cell carcinoma Bacteria Next-generation sequencing |
| dc.subject.keyword.spa.fl_str_mv |
Microbiome Eubiosi Dysbiosi Oral squamous cell carcinoma Bacteria Next-generation sequencing |
| description |
* |
| publishDate |
2021 |
| dc.date.created.none.fl_str_mv |
2021-06-07 |
| dc.date.accessioned.none.fl_str_mv |
2022-05-31T16:09:17Z |
| dc.date.available.none.fl_str_mv |
2022-05-31T16:09:17Z |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/coar/resource_type/c_6501 |
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https://www.openmicrobiologyjournal.com/VOLUME/15/PAGE/98/ABSTRACT/ |
| dc.identifier.issn.spa.fl_str_mv |
1874-2858 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10554/59944 |
| dc.identifier.doi.spa.fl_str_mv |
https://doi.org/10.2174/1874285802115010098 |
| dc.identifier.instname.spa.fl_str_mv |
instname:Pontificia Universidad Javeriana |
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reponame:Repositorio Institucional - Pontificia Universidad Javeriana |
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repourl:https://repository.javeriana.edu.co |
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https://www.openmicrobiologyjournal.com/VOLUME/15/PAGE/98/ABSTRACT/ http://hdl.handle.net/10554/59944 https://doi.org/10.2174/1874285802115010098 |
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1874-2858 instname:Pontificia Universidad Javeriana reponame:Repositorio Institucional - Pontificia Universidad Javeriana repourl:https://repository.javeriana.edu.co |
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eng |
| language |
eng |
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98 |
| dc.relation.citationendpage.spa.fl_str_mv |
110 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Open Microbiology Journal |
| dc.relation.citationvolume.spa.fl_str_mv |
15 |
| dc.rights.licence.*.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
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http://creativecommons.org/licenses/by-nc/4.0/ |
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http://purl.org/coar/access_right/c_abf2 |
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Atribución-NoComercial 4.0 Internacional http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
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Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2Erira, AlveiroGarcía Robayo, Dabeiba AdrianaChalá, Andrés IgnacioMoreno Torres, AndreiMuñoz López, Eliana ElisaArregui, Angel CidTobar, Fabian TosseGamboa Jaimes, Fredy OmarPontificia Universidad Javeriana. Facultad de Medicina. Departamento de Medicina Interna. Medicina InternaPontificia Universidad Javeriana. Facultad de Medicina. Hospital Universitario San Ignacio2022-05-31T16:09:17Z2022-05-31T16:09:17Z2021-06-07https://www.openmicrobiologyjournal.com/VOLUME/15/PAGE/98/ABSTRACT/1874-2858http://hdl.handle.net/10554/59944https://doi.org/10.2174/1874285802115010098instname:Pontificia Universidad Javerianareponame:Repositorio Institucional - Pontificia Universidad Javerianarepourl:https://repository.javeriana.edu.co*Background: Oral squamous cell carcinoma (OSCC) is the sixth most common cancer in the world, and the bacterial microbiome has been considered a risk factor that could play an important role in carcinogenesis. Objective: A bacteriome study was performed by next-generation sequencing in dental plaque, saliva, and tumor samples of 10 OSCC patients and compared with bacteriome in dental plaque and saliva of 10 patients without OSCC. Methods: DNA was extracted from all samples and sequenced by Illumina technology MiSeq™. Bioinformatic analyzes were performed for evaluated sequence quality, alpha and beta diversity, bidirectional analysis of variance (p <0.05), and principal component analysis. After establishing bacterial profiles associated with each sample and population, intragroup and intergroup comparisons were carried out. For bacteria identification compatible with eubiosis and dysbiosis processes, a screening was performed based on the frequency of appearance in all patient samples with and without OSCC. Lastly, frequency, average, standard deviation, Chi-square, and Mann Whitney test were calculated. Results: Out of the identified 1,231 bacteria in the populations under study, 45 bacterial species were selected, of which 34 were compatible with eubiosis, and 11 were compatible with dysbiosis. Among the bacteria compatible with eubiosis were species of Lactobacillus and Streptococcus, Chromobacterium violaceum, Enterobacter asburiae, Mycobacterium chubuense, Mycoplasma penetrans, and Brachyspira intermedia. Among the species associated with dysbiosis, Providencia stuartii, Capnocytophaga canimorsus, Legionella pneumophila, and Mycoplasma hominis were notable. Conclusion: Thirty-four bacterial species may be associated with eubiosis or healthy states and 11 bacterial species could be associated with dysbiosis or pathogenic state, OSCC.https://orcid.org/ 0000-0003-1509-5631https://orcid.org/ 0000-0003-0770-9138https://orcid.org/ 0000-0001-5334-4286Revista Internacional - No indexadaSiPDFapplication/pdfeng*MicrobiomeEubiosiDysbiosiOral squamous cell carcinomaBacteriaNext-generation sequencingBacteriome identified by next-generation sequencing in saliva, dental plaque, and tumor tissue of patients with oral squamous cell carcinomaArtículo de revistahttp://purl.org/coar/resource_type/c_650198110Open Microbiology Journal15ORIGINALTOMICROJ-15-98.pdfTOMICROJ-15-98.pdfArtículoapplication/pdf2721841http://repository.javeriana.edu.co/bitstream/10554/59944/1/TOMICROJ-15-98.pdf6f9103a16ecc37cd8bbf2ba6cd0fb832MD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-82603http://repository.javeriana.edu.co/bitstream/10554/59944/2/license.txt2070d280cc89439d983d9eee1b17df53MD52open accessTHUMBNAILTOMICROJ-15-98.pdf.jpgTOMICROJ-15-98.pdf.jpgIM Thumbnailimage/jpeg9196http://repository.javeriana.edu.co/bitstream/10554/59944/3/TOMICROJ-15-98.pdf.jpg638f6888c3e124c165b5d915666c533bMD53open access10554/59944oai:repository.javeriana.edu.co:10554/599442022-06-01 03:04:25.783Repositorio Institucional - Pontificia Universidad Javerianarepositorio@javeriana.edu.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 |