Regulatory Role of the RUNX2 Transcription Factor in Lung Cancer Apoptosis
Q4
- Autores:
-
Bernal Forigua, Camila
Otálora-Otálora, Beatriz Andrea
Canas, Alejandra
Barreto, Alfonso
Prieto, Karol
Montecino, Martín
Rojas, Adriana
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2022
- Institución:
- Pontificia Universidad Javeriana
- Repositorio:
- Repositorio Universidad Javeriana
- Idioma:
- eng
- OAI Identifier:
- oai:repository.javeriana.edu.co:10554/65318
- Acceso en línea:
- https://www.hindawi.com/journals/ijcb/2022/5198203/#abstract
http://hdl.handle.net/10554/65318
https://doi.org/10.1155/2022/5198203
- Palabra clave:
- Rights
- License
- Atribución-NoComercial 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Regulatory Role of the RUNX2 Transcription Factor in Lung Cancer Apoptosis |
| title |
Regulatory Role of the RUNX2 Transcription Factor in Lung Cancer Apoptosis |
| spellingShingle |
Regulatory Role of the RUNX2 Transcription Factor in Lung Cancer Apoptosis |
| title_short |
Regulatory Role of the RUNX2 Transcription Factor in Lung Cancer Apoptosis |
| title_full |
Regulatory Role of the RUNX2 Transcription Factor in Lung Cancer Apoptosis |
| title_fullStr |
Regulatory Role of the RUNX2 Transcription Factor in Lung Cancer Apoptosis |
| title_full_unstemmed |
Regulatory Role of the RUNX2 Transcription Factor in Lung Cancer Apoptosis |
| title_sort |
Regulatory Role of the RUNX2 Transcription Factor in Lung Cancer Apoptosis |
| dc.creator.fl_str_mv |
Bernal Forigua, Camila Otálora-Otálora, Beatriz Andrea Canas, Alejandra Barreto, Alfonso Prieto, Karol Montecino, Martín Rojas, Adriana |
| dc.contributor.author.none.fl_str_mv |
Bernal Forigua, Camila Otálora-Otálora, Beatriz Andrea Canas, Alejandra Barreto, Alfonso Prieto, Karol Montecino, Martín Rojas, Adriana |
| dc.contributor.corporatename.spa.fl_str_mv |
Pontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana. Grupo de investigación Instituto de Genética Humana |
| dc.contributor.corporatename.none.fl_str_mv |
Pontificia Universidad Javeriana. Facultad de Medicina. Departamento de Medicina Interna. Neumología |
| dc.contributor.javerianateacher.none.fl_str_mv |
Canas, Alejandra Rojas, Adriana |
| description |
Q4 |
| publishDate |
2022 |
| dc.date.created.none.fl_str_mv |
2022-12-03 |
| dc.date.accessioned.none.fl_str_mv |
2023-08-28T14:58:45Z |
| dc.date.available.none.fl_str_mv |
2023-08-28T14:58:45Z |
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Artículo de revista |
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https://www.hindawi.com/journals/ijcb/2022/5198203/#abstract |
| dc.identifier.issn.spa.fl_str_mv |
1687-8876 / 1687-8884 (Electrónico) |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10554/65318 |
| dc.identifier.doi.spa.fl_str_mv |
https://doi.org/10.1155/2022/5198203 |
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instname:Pontificia Universidad Javeriana |
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reponame:Repositorio Institucional - Pontificia Universidad Javeriana |
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repourl:https://repository.javeriana.edu.co |
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https://www.hindawi.com/journals/ijcb/2022/5198203/#abstract http://hdl.handle.net/10554/65318 https://doi.org/10.1155/2022/5198203 |
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1687-8876 / 1687-8884 (Electrónico) instname:Pontificia Universidad Javeriana reponame:Repositorio Institucional - Pontificia Universidad Javeriana repourl:https://repository.javeriana.edu.co |
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eng |
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eng |
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1 |
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13 |
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International Journal of Cell Biology |
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Atribución-NoComercial 4.0 Internacional |
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http://creativecommons.org/licenses/by-nc/4.0/ |
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Atribución-NoComercial 4.0 Internacional http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
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PDF |
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application/pdf |
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Colombia |
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Bogotá (Colombia) |
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Pontificia Universidad Javeriana |
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Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2Bernal Forigua, CamilaOtálora-Otálora, Beatriz AndreaCanas, AlejandraBarreto, AlfonsoPrieto, KarolMontecino, MartínRojas, AdrianaPontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana. Grupo de investigación Instituto de Genética HumanaPontificia Universidad Javeriana. Facultad de Medicina. Departamento de Medicina Interna. NeumologíaCanas, AlejandraRojas, AdrianaColombiaBogotá (Colombia)2023-08-28T14:58:45Z2023-08-28T14:58:45Z2022-12-03https://www.hindawi.com/journals/ijcb/2022/5198203/#abstract1687-8876 / 1687-8884 (Electrónico)http://hdl.handle.net/10554/65318https://doi.org/10.1155/2022/5198203instname:Pontificia Universidad Javerianareponame:Repositorio Institucional - Pontificia Universidad Javerianarepourl:https://repository.javeriana.edu.coPDFapplication/pdfengRegulatory Role of the RUNX2 Transcription Factor in Lung Cancer ApoptosisArtículo de revistahttp://purl.org/coar/resource_type/c_2df8fbb1Q4Pacientes con Cáncer de pulmónLung cancer is the leading cause of cancer death globally. Numerous factors intervene in the onset and progression of lung tumors, among which the participation of lineage-specific transcription factors stands out. Several transcription factors important in embryonic development are abnormally expressed in adult tissues and thus participate in the activation of signaling pathways related to the acquisition of the tumor phenotype. RUNX2 is the transcription factor responsible for osteogenic differentiation in mammals. Current studies have confirmed that RUNX2 is closely related to the proliferation, invasion, and bone metastasis of multiple cancer types, such as osteosarcoma, breast cancer (BC), prostate cancer, gastric cancer, colorectal cancer, and lung cancer. Thus, the present study is aimed at evaluating the role of the RUNX2 transcription factor in inhibiting the apoptosis process. Loss-of-function assays using sh-RNA from lentiviral particles and coupled with Annexin/propidium iodide (PI) assays (flow cytometry), immunofluorescence, and quantitative PCR analysis of genes related to cell apoptosis (BAD, BAX, BCL2, BCL-XL, and MCL1) were performed. Silencing assays and Annexin/PI assays demonstrated that when RUNX2 was absent, the percentage of dead cells increased, and the expression levels of the BCL2, BCL-XL, and MCL1 genes were downregulated. Furthermore, to confirm whether the regulatory role of RUNX2 in the expression of these genes is related to its binding to the promoter region, we performed chromatin immunoprecipitation (ChIP) assays. Here, we report that overexpression of the RUNX2 gene in lung cancer may be related to the inhibition of the intrinsic apoptosis pathway, specifically, through direct transcriptional regulation of the antiapoptotic gene BCL2 and indirect regulation of BCL-XL and MCL1.https://orcid.org/0000-0003-1555-6661https://orcid.org/0000-0002-5668-0266https://orcid.org/0000-0003-3975-2835https://orcid.org/0000-0001-8528-4433Revista Internacional - IndexadaCNo113International Journal of Cell BiologyORIGINALRegulatory Role of the RUNX2 Transcription Factor in Lung Cancer Apoptosis.pdfRegulatory Role of the RUNX2 Transcription Factor in Lung Cancer Apoptosis.pdfArtículoapplication/pdf4218368http://repository.javeriana.edu.co/bitstream/10554/65318/1/Regulatory%20Role%20of%20the%20RUNX2%20Transcription%20Factor%20in%20Lung%20Cancer%20Apoptosis.pdf4a294f7930888deac5f07ae82e8a72f8MD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-82603http://repository.javeriana.edu.co/bitstream/10554/65318/2/license.txt2070d280cc89439d983d9eee1b17df53MD52open accessTHUMBNAILRegulatory Role of the RUNX2 Transcription Factor in Lung Cancer Apoptosis.pdf.jpgRegulatory Role of the RUNX2 Transcription Factor in Lung Cancer Apoptosis.pdf.jpgIM Thumbnailimage/jpeg9226http://repository.javeriana.edu.co/bitstream/10554/65318/3/Regulatory%20Role%20of%20the%20RUNX2%20Transcription%20Factor%20in%20Lung%20Cancer%20Apoptosis.pdf.jpg4b17b3328eb7b37c03e29309469f02d1MD53open access10554/65318oai:repository.javeriana.edu.co:10554/653182023-09-06 17:17:40.989Repositorio Institucional - Pontificia Universidad Javerianarepositorio@javeriana.edu.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 |