Growth and developmental effects of DNA cytosine demethylation in two populations of the non-model species Geranium lucidum

Epigenetics is defined as the study of heritable changes in gene expression and function without being associated with any alteration of the underlying DNA sequence. Epigenetic mechanisms are quite sensitive to environmental stimuli, thereby they may provide a second system of heritable variation fo...

Full description

Autores:
Zúñiga Valencia, Federico
Tipo de recurso:
Trabajo de grado de pregrado
Fecha de publicación:
2021
Institución:
Pontificia Universidad Javeriana
Repositorio:
Repositorio Universidad Javeriana
Idioma:
spa
OAI Identifier:
oai:repository.javeriana.edu.co:10554/59481
Acceso en línea:
http://hdl.handle.net/10554/59481
Palabra clave:
Demethylation
Epigenetics
Geranium lucidum
Population
Methylation
Methyl-cytosine
Azacytidine
Zebularine
Biología - Tesis y disertaciones académicas
Metilación de ADN
Epigenética
Regulación genética
Rights
embargoedAccess
License
Atribución-NoComercial-SinDerivadas 4.0 Internacional
id JAVERIANA2_aa9d7c448864fbde6c038191b6809fd4
oai_identifier_str oai:repository.javeriana.edu.co:10554/59481
network_acronym_str JAVERIANA2
network_name_str Repositorio Universidad Javeriana
repository_id_str
dc.title.spa.fl_str_mv Growth and developmental effects of DNA cytosine demethylation in two populations of the non-model species Geranium lucidum
title Growth and developmental effects of DNA cytosine demethylation in two populations of the non-model species Geranium lucidum
spellingShingle Growth and developmental effects of DNA cytosine demethylation in two populations of the non-model species Geranium lucidum
Demethylation
Epigenetics
Geranium lucidum
Population
Methylation
Methyl-cytosine
Azacytidine
Zebularine
Biología - Tesis y disertaciones académicas
Metilación de ADN
Epigenética
Regulación genética
title_short Growth and developmental effects of DNA cytosine demethylation in two populations of the non-model species Geranium lucidum
title_full Growth and developmental effects of DNA cytosine demethylation in two populations of the non-model species Geranium lucidum
title_fullStr Growth and developmental effects of DNA cytosine demethylation in two populations of the non-model species Geranium lucidum
title_full_unstemmed Growth and developmental effects of DNA cytosine demethylation in two populations of the non-model species Geranium lucidum
title_sort Growth and developmental effects of DNA cytosine demethylation in two populations of the non-model species Geranium lucidum
dc.creator.fl_str_mv Zúñiga Valencia, Federico
dc.contributor.advisor.none.fl_str_mv Terán Pérez, Wilson
Alonso, Conchita
dc.contributor.author.none.fl_str_mv Zúñiga Valencia, Federico
dc.contributor.evaluator.none.fl_str_mv Márquez Cardona, Maria Del Pilar
dc.subject.keyword.spa.fl_str_mv Demethylation
Epigenetics
Geranium lucidum
Population
Methylation
Methyl-cytosine
Azacytidine
Zebularine
topic Demethylation
Epigenetics
Geranium lucidum
Population
Methylation
Methyl-cytosine
Azacytidine
Zebularine
Biología - Tesis y disertaciones académicas
Metilación de ADN
Epigenética
Regulación genética
dc.subject.armarc.spa.fl_str_mv Biología - Tesis y disertaciones académicas
Metilación de ADN
Epigenética
Regulación genética
description Epigenetics is defined as the study of heritable changes in gene expression and function without being associated with any alteration of the underlying DNA sequence. Epigenetic mechanisms are quite sensitive to environmental stimuli, thereby they may provide a second system of heritable variation for natural selection to act upon. In plants, DNA methylation usually addresses ecological and evolutionary processes such as phenotypic plasticity and transgenerational effects. A suitable technique used to dissect the contribution of this epigenetic mark on those processes is the experimental DNA demethylation since it can be applied to both model and non-model species. In this study, we compare two experimental demethylation protocols on the non-model species Geranium lucidum, a noxious and invasive herb, to determine which of them is more efficient in removing DNA methylation marks, as well as altering growth and developmental phenotypes. Seeds from two geographically separated populations were subjected to two distinct demethylation protocols (seed germination and leaf infiltration). Leaf infiltration proved to be the most suitable protocol to reduce methylated DNA, while the germination protocol was unable to alter methylation levels. However, both treatments were unable to induce significant changes in the plant's phenotype. In conclusion, leaf infiltration is the best protocol to carry out future epigenetics research in this non-model species.
publishDate 2021
dc.date.created.none.fl_str_mv 2021-06-25
dc.date.accessioned.none.fl_str_mv 2022-04-08T17:59:12Z
dc.date.available.none.fl_str_mv 2022-04-08T17:59:12Z
dc.type.local.spa.fl_str_mv Tesis/Trabajo de grado - Monografía - Pregrado
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_7a1f
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/bachelorThesis
format http://purl.org/coar/resource_type/c_7a1f
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10554/59481
dc.identifier.instname.spa.fl_str_mv instname:Pontificia Universidad Javeriana
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional - Pontificia Universidad Javeriana
dc.identifier.repourl.spa.fl_str_mv repourl:https://repository.javeriana.edu.co
url http://hdl.handle.net/10554/59481
identifier_str_mv instname:Pontificia Universidad Javeriana
reponame:Repositorio Institucional - Pontificia Universidad Javeriana
repourl:https://repository.javeriana.edu.co
dc.language.iso.spa.fl_str_mv spa
language spa
dc.rights.licence.*.fl_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
dc.rights.coar.spa.fl_str_mv http://purl.org/coar/access_right/c_f1cf
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
http://purl.org/coar/access_right/c_f1cf
eu_rights_str_mv embargoedAccess
dc.format.spa.fl_str_mv PDF
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.coverage.spatial.spa.fl_str_mv Colombia
dc.coverage.temporal.none.fl_str_mv 2017-2021
dc.coverage.department.spa.fl_str_mv Cundinamarca (Colombia)
dc.coverage.region.spa.fl_str_mv Bogotá, D.C. (Bogotá, Colombia)
dc.publisher.spa.fl_str_mv Pontificia Universidad Javeriana
dc.publisher.program.spa.fl_str_mv Biología
dc.publisher.faculty.spa.fl_str_mv Facultad de Ciencias
institution Pontificia Universidad Javeriana
bitstream.url.fl_str_mv http://repository.javeriana.edu.co/bitstream/10554/59481/4/license.txt
http://repository.javeriana.edu.co/bitstream/10554/59481/1/Growth%20and%20developmental%20effects%20of%20cytosine%20demethylation%20in%20two%20populations%20of%20the%20non.pdf
http://repository.javeriana.edu.co/bitstream/10554/59481/2/Formato%20Carta%20firmas%20trabajo%20de%20grado%20Federico%20Zu%cc%81n%cc%83iga.pdf
http://repository.javeriana.edu.co/bitstream/10554/59481/3/Carta%20autorizacio%cc%81n%20autores.pdf
http://repository.javeriana.edu.co/bitstream/10554/59481/5/Growth%20and%20developmental%20effects%20of%20cytosine%20demethylation%20in%20two%20populations%20of%20the%20non.pdf.jpg
http://repository.javeriana.edu.co/bitstream/10554/59481/6/Formato%20Carta%20firmas%20trabajo%20de%20grado%20Federico%20Zu%cc%81n%cc%83iga.pdf.jpg
http://repository.javeriana.edu.co/bitstream/10554/59481/7/Carta%20autorizacio%cc%81n%20autores.pdf.jpg
bitstream.checksum.fl_str_mv 2070d280cc89439d983d9eee1b17df53
2e902a9cc5db6b01972b1ccdc391fe4e
c6a3a1b8890fb4291b2a1f3d6c1b5425
5b5d43a7b0d8fcb0a93504b4b68d49f6
e4aaaf4592513e43324aed05488f6a16
f6211a44646663f2f22d37bed0ee033d
bcdae2b9fe4668b340179e41182c1543
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositorio Institucional - Pontificia Universidad Javeriana
repository.mail.fl_str_mv repositorio@javeriana.edu.co
_version_ 1814337731844636672
spelling Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/embargoedAccessDe acuerdo con la naturaleza del uso concedido, la presente licencia parcial se otorga a título gratuito por el máximo tiempo legal colombiano, con el propósito de que en dicho lapso mi (nuestra) obra sea explotada en las condiciones aquí estipuladas y para los fines indicados, respetando siempre la titularidad de los derechos patrimoniales y morales correspondientes, de acuerdo con los usos honrados, de manera proporcional y justificada a la finalidad perseguida, sin ánimo de lucro ni de comercialización. De manera complementaria, garantizo (garantizamos) en mi (nuestra) calidad de estudiante (s) y por ende autor (es) exclusivo (s), que la Tesis o Trabajo de Grado en cuestión, es producto de mi (nuestra) plena autoría, de mi (nuestro) esfuerzo personal intelectual, como consecuencia de mi (nuestra) creación original particular y, por tanto, soy (somos) el (los) único (s) titular (es) de la misma. Además, aseguro (aseguramos) que no contiene citas, ni transcripciones de otras obras protegidas, por fuera de los límites autorizados por la ley, según los usos honrados, y en proporción a los fines previstos; ni tampoco contempla declaraciones difamatorias contra terceros; respetando el derecho a la imagen, intimidad, buen nombre y demás derechos constitucionales. Adicionalmente, manifiesto (manifestamos) que no se incluyeron expresiones contrarias al orden público ni a las buenas costumbres. En consecuencia, la responsabilidad directa en la elaboración, presentación, investigación y, en general, contenidos de la Tesis o Trabajo de Grado es de mí (nuestro) competencia exclusiva, eximiendo de toda responsabilidad a la Pontifica Universidad Javeriana por tales aspectos. Sin perjuicio de los usos y atribuciones otorgadas en virtud de este documento, continuaré (continuaremos) conservando los correspondientes derechos patrimoniales sin modificación o restricción alguna, puesto que, de acuerdo con la legislación colombiana aplicable, el presente es un acuerdo jurídico que en ningún caso conlleva la enajenación de los derechos patrimoniales derivados del régimen del Derecho de Autor. De conformidad con lo establecido en el artículo 30 de la Ley 23 de 1982 y el artículo 11 de la Decisión Andina 351 de 1993, "Los derechos morales sobre el trabajo son propiedad de los autores", los cuales son irrenunciables, imprescriptibles, inembargables e inalienables. En consecuencia, la Pontificia Universidad Javeriana está en la obligación de RESPETARLOS Y HACERLOS RESPETAR, para lo cual tomará las medidas correspondientes para garantizar su observancia.http://purl.org/coar/access_right/c_f1cfTerán Pérez, WilsonAlonso, ConchitaZúñiga Valencia, FedericoMárquez Cardona, Maria Del PilarColombia2017-2021Cundinamarca (Colombia)Bogotá, D.C. (Bogotá, Colombia)2022-04-08T17:59:12Z2022-04-08T17:59:12Z2021-06-25http://hdl.handle.net/10554/59481instname:Pontificia Universidad Javerianareponame:Repositorio Institucional - Pontificia Universidad Javerianarepourl:https://repository.javeriana.edu.coPDFapplication/pdfspaPontificia Universidad JaverianaBiologíaFacultad de CienciasGrowth and developmental effects of DNA cytosine demethylation in two populations of the non-model species Geranium lucidumTesis/Trabajo de grado - Monografía - Pregradohttp://purl.org/coar/resource_type/c_7a1finfo:eu-repo/semantics/bachelorThesisDemethylationEpigeneticsGeranium lucidumPopulationMethylationMethyl-cytosineAzacytidineZebularineBiología - Tesis y disertaciones académicasMetilación de ADNEpigenéticaRegulación genéticaEpigenetics is defined as the study of heritable changes in gene expression and function without being associated with any alteration of the underlying DNA sequence. Epigenetic mechanisms are quite sensitive to environmental stimuli, thereby they may provide a second system of heritable variation for natural selection to act upon. In plants, DNA methylation usually addresses ecological and evolutionary processes such as phenotypic plasticity and transgenerational effects. A suitable technique used to dissect the contribution of this epigenetic mark on those processes is the experimental DNA demethylation since it can be applied to both model and non-model species. In this study, we compare two experimental demethylation protocols on the non-model species Geranium lucidum, a noxious and invasive herb, to determine which of them is more efficient in removing DNA methylation marks, as well as altering growth and developmental phenotypes. Seeds from two geographically separated populations were subjected to two distinct demethylation protocols (seed germination and leaf infiltration). Leaf infiltration proved to be the most suitable protocol to reduce methylated DNA, while the germination protocol was unable to alter methylation levels. However, both treatments were unable to induce significant changes in the plant's phenotype. In conclusion, leaf infiltration is the best protocol to carry out future epigenetics research in this non-model species.Biólogo (a)PregradoLICENSElicense.txtlicense.txttext/plain; charset=utf-82603http://repository.javeriana.edu.co/bitstream/10554/59481/4/license.txt2070d280cc89439d983d9eee1b17df53MD54open accessORIGINALGrowth and developmental effects of cytosine demethylation in two populations of the non.pdfGrowth and developmental effects of cytosine demethylation in two populations of the non.pdfDocumentoapplication/pdf831645http://repository.javeriana.edu.co/bitstream/10554/59481/1/Growth%20and%20developmental%20effects%20of%20cytosine%20demethylation%20in%20two%20populations%20of%20the%20non.pdf2e902a9cc5db6b01972b1ccdc391fe4eMD51open accessFormato Carta firmas trabajo de grado Federico Zúñiga.pdfFormato Carta firmas trabajo de grado Federico Zúñiga.pdfCarta probación directoresapplication/pdf414977http://repository.javeriana.edu.co/bitstream/10554/59481/2/Formato%20Carta%20firmas%20trabajo%20de%20grado%20Federico%20Zu%cc%81n%cc%83iga.pdfc6a3a1b8890fb4291b2a1f3d6c1b5425MD52metadata only accessCarta autorización autores.pdfCarta autorización autores.pdfLicencia de usoapplication/pdf787042http://repository.javeriana.edu.co/bitstream/10554/59481/3/Carta%20autorizacio%cc%81n%20autores.pdf5b5d43a7b0d8fcb0a93504b4b68d49f6MD53metadata only accessTHUMBNAILGrowth and developmental effects of cytosine demethylation in two populations of the non.pdf.jpgGrowth and developmental effects of cytosine demethylation in two populations of the non.pdf.jpgIM Thumbnailimage/jpeg6945http://repository.javeriana.edu.co/bitstream/10554/59481/5/Growth%20and%20developmental%20effects%20of%20cytosine%20demethylation%20in%20two%20populations%20of%20the%20non.pdf.jpge4aaaf4592513e43324aed05488f6a16MD55open accessFormato Carta firmas trabajo de grado Federico Zúñiga.pdf.jpgFormato Carta firmas trabajo de grado Federico Zúñiga.pdf.jpgIM Thumbnailimage/jpeg5485http://repository.javeriana.edu.co/bitstream/10554/59481/6/Formato%20Carta%20firmas%20trabajo%20de%20grado%20Federico%20Zu%cc%81n%cc%83iga.pdf.jpgf6211a44646663f2f22d37bed0ee033dMD56open accessCarta autorización autores.pdf.jpgCarta autorización autores.pdf.jpgIM Thumbnailimage/jpeg8209http://repository.javeriana.edu.co/bitstream/10554/59481/7/Carta%20autorizacio%cc%81n%20autores.pdf.jpgbcdae2b9fe4668b340179e41182c1543MD57open access10554/59481oai:repository.javeriana.edu.co:10554/594812022-05-02 12:35:22.52Repositorio Institucional - Pontificia Universidad Javerianarepositorio@javeriana.edu.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

Publicaciones similares