The flavonoid naringenin has been investigated as a possible vir gene inducer in Agrobacterium mediated transformation in Passiflora mollissima, P. giberti and Nicotiana tabacum cv. Xanthi. Thetransformation efficiency percentage of explants showing blue GUS expression and the extent of staining fol...
- Autores:
-
Cancino, G. O.; Departamento de Biología Facultad de Ciencias Pontificia Universidad Javeriana, Bogotá
Gill, M. I.S.; Department of Genetic and Biotechnology, P.A.U., Ludhiana, India
Davey, M. R.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK
Power, J. B.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK
Lowe, K. C.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2004
- Institución:
- Pontificia Universidad Javeriana
- Repositorio:
- Repositorio Universidad Javeriana
- Idioma:
- eng
- OAI Identifier:
- oai:repository.javeriana.edu.co:10554/31943
- Acceso en línea:
- http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4922
http://hdl.handle.net/10554/31943
- Palabra clave:
- null
naringenin; Agrobacterium; acetosyringone; Passifloras
null
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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Atribución-NoComercial-SinDerivadas 4.0 Internacionalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2nullCancino, G. O.; Departamento de Biología Facultad de Ciencias Pontificia Universidad Javeriana, BogotáGill, M. I.S.; Department of Genetic and Biotechnology, P.A.U., Ludhiana, IndiaDavey, M. R.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UKPower, J. B.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UKLowe, K. C.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK2018-02-24T16:01:01Z2020-04-15T18:08:45Z2018-02-24T16:01:01Z2020-04-15T18:08:45Z2004-01-10http://revistas.javeriana.edu.co/index.php/scientarium/article/view/49222027-13520122-7483http://hdl.handle.net/10554/31943PDFapplication/pdfengPontificia Universidad Javerianahttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/4922/3797Universitas Scientiarum; Vol 9, No 1 (2004); 47-57Universitas Scientiarum; Vol 9, No 1 (2004); 47-57Universitas Scientiarum; Vol 9, No 1 (2004); 47-57nullnaringenin; Agrobacterium; acetosyringone; Passiflorasnullnullnullnullhttp://purl.org/coar/version/c_970fb48d4fbd8a85Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleNARINGENIN ENHANCED EFFICIENCY OF GUS ACTIVITY IN Passiflora mollissima (H.B.K.) BaileyThe flavonoid naringenin has been investigated as a possible vir gene inducer in Agrobacterium mediated transformation in Passiflora mollissima, P. giberti and Nicotiana tabacum cv. Xanthi. Thetransformation efficiency percentage of explants showing blue GUS expression and the extent of staining following inoculation with Agrobacterium tumefaciens strains EHA 105 and 1065, carrying gus and nptII genes was enhanced with the supplementation of the co-cultivation medium with naringenin. Supplementation of medium with 100mM (strain EHA 105) and 300 mM (strain 1065) naringenin was most effective at enhancing mean (±s.e.m., n=3) GUS activity in leaf explants (20.3± 2.4%, strain EHA; 105; 6.0 ± 0.57%, strain 1065) and nodal segments (16.7 ± 2.4% strain EHA 105; 8.3 ± 0.57% strain 1065) of P. mollissima. In P. giberti and N. tabacum maximum GUS activitywas obtained in leaf and root explants with 100mM naringenin for both strains analysed. Additionally, when naringenin was added to Luria Bertani (LB) medium, both bacterial growth via optical density and colony forming units were higher when compared to control. This is the first report of the use ofnaringenin to enhance gene transfer from Agrobacterium to plants. These findings suggest that naringenin can be used as an alternative to acetosyringone for vir gene induction in Agrobacterium. This approachmay be especially useful in plants that are generally recalcitrant to Agrobacterium-mediated transformation.10554/31943oai:repository.javeriana.edu.co:10554/319432023-03-28 16:15:56.861Repositorio Institucional - Pontificia Universidad Javerianarepositorio@javeriana.edu.co |
dc.title.english.eng.fl_str_mv |
NARINGENIN ENHANCED EFFICIENCY OF GUS ACTIVITY IN Passiflora mollissima (H.B.K.) Bailey |
dc.creator.fl_str_mv |
Cancino, G. O.; Departamento de Biología Facultad de Ciencias Pontificia Universidad Javeriana, Bogotá Gill, M. I.S.; Department of Genetic and Biotechnology, P.A.U., Ludhiana, India Davey, M. R.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK Power, J. B.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK Lowe, K. C.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK |
dc.contributor.author.none.fl_str_mv |
Cancino, G. O.; Departamento de Biología Facultad de Ciencias Pontificia Universidad Javeriana, Bogotá Gill, M. I.S.; Department of Genetic and Biotechnology, P.A.U., Ludhiana, India Davey, M. R.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK Power, J. B.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK Lowe, K. C.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK |
dc.contributor.none.fl_str_mv |
null |
dc.subject.eng.fl_str_mv |
null naringenin; Agrobacterium; acetosyringone; Passifloras null |
topic |
null naringenin; Agrobacterium; acetosyringone; Passifloras null |
spellingShingle |
null naringenin; Agrobacterium; acetosyringone; Passifloras null |
description |
The flavonoid naringenin has been investigated as a possible vir gene inducer in Agrobacterium mediated transformation in Passiflora mollissima, P. giberti and Nicotiana tabacum cv. Xanthi. Thetransformation efficiency percentage of explants showing blue GUS expression and the extent of staining following inoculation with Agrobacterium tumefaciens strains EHA 105 and 1065, carrying gus and nptII genes was enhanced with the supplementation of the co-cultivation medium with naringenin. Supplementation of medium with 100mM (strain EHA 105) and 300 mM (strain 1065) naringenin was most effective at enhancing mean (±s.e.m., n=3) GUS activity in leaf explants (20.3± 2.4%, strain EHA; 105; 6.0 ± 0.57%, strain 1065) and nodal segments (16.7 ± 2.4% strain EHA 105; 8.3 ± 0.57% strain 1065) of P. mollissima. In P. giberti and N. tabacum maximum GUS activitywas obtained in leaf and root explants with 100mM naringenin for both strains analysed. Additionally, when naringenin was added to Luria Bertani (LB) medium, both bacterial growth via optical density and colony forming units were higher when compared to control. This is the first report of the use ofnaringenin to enhance gene transfer from Agrobacterium to plants. These findings suggest that naringenin can be used as an alternative to acetosyringone for vir gene induction in Agrobacterium. This approachmay be especially useful in plants that are generally recalcitrant to Agrobacterium-mediated transformation. |
publishDate |
2004 |
dc.date.created.none.fl_str_mv |
2004-01-10 |
dc.date.accessioned.none.fl_str_mv |
2018-02-24T16:01:01Z 2020-04-15T18:08:45Z |
dc.date.available.none.fl_str_mv |
2018-02-24T16:01:01Z 2020-04-15T18:08:45Z |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.hasversion.none.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.local.spa.fl_str_mv |
Artículo de revista |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
format |
http://purl.org/coar/resource_type/c_6501 |
dc.identifier.none.fl_str_mv |
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4922 |
dc.identifier.issn.none.fl_str_mv |
2027-1352 0122-7483 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10554/31943 |
url |
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4922 http://hdl.handle.net/10554/31943 |
identifier_str_mv |
2027-1352 0122-7483 |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.relation.uri.none.fl_str_mv |
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4922/3797 |
dc.relation.citationissue.eng.fl_str_mv |
Universitas Scientiarum; Vol 9, No 1 (2004); 47-57 |
dc.relation.citationissue.spa.fl_str_mv |
Universitas Scientiarum; Vol 9, No 1 (2004); 47-57 |
dc.relation.citationissue.por.fl_str_mv |
Universitas Scientiarum; Vol 9, No 1 (2004); 47-57 |
dc.rights.licence.*.fl_str_mv |
Atribución-NoComercial-SinDerivadas 4.0 Internacional |
dc.rights.accessrights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.coar.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
rights_invalid_str_mv |
Atribución-NoComercial-SinDerivadas 4.0 Internacional http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.spa.fl_str_mv |
PDF |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
null null null |
dc.publisher.eng.fl_str_mv |
Pontificia Universidad Javeriana |
institution |
Pontificia Universidad Javeriana |
repository.name.fl_str_mv |
Repositorio Institucional - Pontificia Universidad Javeriana |
repository.mail.fl_str_mv |
repositorio@javeriana.edu.co |
_version_ |
1811671363055779840 |