The flavonoid naringenin has been investigated as a possible vir gene inducer in Agrobacterium mediated transformation in Passiflora mollissima, P. giberti and Nicotiana tabacum cv. Xanthi. Thetransformation efficiency percentage of explants showing blue GUS expression and the extent of staining fol...

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Autores:
Cancino, G. O.; Departamento de Biología Facultad de Ciencias Pontificia Universidad Javeriana, Bogotá
Gill, M. I.S.; Department of Genetic and Biotechnology, P.A.U., Ludhiana, India
Davey, M. R.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK
Power, J. B.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK
Lowe, K. C.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK
Tipo de recurso:
Article of journal
Fecha de publicación:
2004
Institución:
Pontificia Universidad Javeriana
Repositorio:
Repositorio Universidad Javeriana
Idioma:
eng
OAI Identifier:
oai:repository.javeriana.edu.co:10554/31943
Acceso en línea:
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4922
http://hdl.handle.net/10554/31943
Palabra clave:
null
naringenin; Agrobacterium; acetosyringone; Passifloras
null
Rights
openAccess
License
Atribución-NoComercial-SinDerivadas 4.0 Internacional
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oai_identifier_str oai:repository.javeriana.edu.co:10554/31943
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network_name_str Repositorio Universidad Javeriana
repository_id_str
spelling Atribución-NoComercial-SinDerivadas 4.0 Internacionalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2nullCancino, G. O.; Departamento de Biología Facultad de Ciencias Pontificia Universidad Javeriana, BogotáGill, M. I.S.; Department of Genetic and Biotechnology, P.A.U., Ludhiana, IndiaDavey, M. R.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UKPower, J. B.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UKLowe, K. C.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK2018-02-24T16:01:01Z2020-04-15T18:08:45Z2018-02-24T16:01:01Z2020-04-15T18:08:45Z2004-01-10http://revistas.javeriana.edu.co/index.php/scientarium/article/view/49222027-13520122-7483http://hdl.handle.net/10554/31943PDFapplication/pdfengPontificia Universidad Javerianahttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/4922/3797Universitas Scientiarum; Vol 9, No 1 (2004); 47-57Universitas Scientiarum; Vol 9, No 1 (2004); 47-57Universitas Scientiarum; Vol 9, No 1 (2004); 47-57nullnaringenin; Agrobacterium; acetosyringone; Passiflorasnullnullnullnullhttp://purl.org/coar/version/c_970fb48d4fbd8a85Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleNARINGENIN ENHANCED EFFICIENCY OF GUS ACTIVITY IN Passiflora mollissima (H.B.K.) BaileyThe flavonoid naringenin has been investigated as a possible vir gene inducer in Agrobacterium mediated transformation in Passiflora mollissima, P. giberti and Nicotiana tabacum cv. Xanthi. Thetransformation efficiency percentage of explants showing blue GUS expression and the extent of staining following inoculation with Agrobacterium tumefaciens strains EHA 105 and 1065, carrying gus and nptII genes was enhanced with the supplementation of the co-cultivation medium with naringenin. Supplementation of medium with 100mM (strain EHA 105) and 300 mM (strain 1065) naringenin was most effective at enhancing mean (±s.e.m., n=3) GUS activity in leaf explants (20.3± 2.4%, strain EHA; 105; 6.0 ± 0.57%, strain 1065) and nodal segments (16.7 ± 2.4% strain EHA 105; 8.3 ± 0.57% strain 1065) of P. mollissima. In P. giberti and N. tabacum maximum GUS activitywas obtained in leaf and root explants with 100mM naringenin for both strains analysed. Additionally, when naringenin was added to Luria Bertani (LB) medium, both bacterial growth via optical density and colony forming units were higher when compared to control. This is the first report of the use ofnaringenin to enhance gene transfer from Agrobacterium to plants. These findings suggest that naringenin can be used as an alternative to acetosyringone for vir gene induction in Agrobacterium. This approachmay be especially useful in plants that are generally recalcitrant to Agrobacterium-mediated transformation.10554/31943oai:repository.javeriana.edu.co:10554/319432023-03-28 16:15:56.861Repositorio Institucional - Pontificia Universidad Javerianarepositorio@javeriana.edu.co
dc.title.english.eng.fl_str_mv NARINGENIN ENHANCED EFFICIENCY OF GUS ACTIVITY IN Passiflora mollissima (H.B.K.) Bailey
dc.creator.fl_str_mv Cancino, G. O.; Departamento de Biología Facultad de Ciencias Pontificia Universidad Javeriana, Bogotá
Gill, M. I.S.; Department of Genetic and Biotechnology, P.A.U., Ludhiana, India
Davey, M. R.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK
Power, J. B.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK
Lowe, K. C.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK
dc.contributor.author.none.fl_str_mv Cancino, G. O.; Departamento de Biología Facultad de Ciencias Pontificia Universidad Javeriana, Bogotá
Gill, M. I.S.; Department of Genetic and Biotechnology, P.A.U., Ludhiana, India
Davey, M. R.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK
Power, J. B.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK
Lowe, K. C.; School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 RD, UK
dc.contributor.none.fl_str_mv null
dc.subject.eng.fl_str_mv null
naringenin; Agrobacterium; acetosyringone; Passifloras
null
topic null
naringenin; Agrobacterium; acetosyringone; Passifloras
null
spellingShingle null
naringenin; Agrobacterium; acetosyringone; Passifloras
null
description The flavonoid naringenin has been investigated as a possible vir gene inducer in Agrobacterium mediated transformation in Passiflora mollissima, P. giberti and Nicotiana tabacum cv. Xanthi. Thetransformation efficiency percentage of explants showing blue GUS expression and the extent of staining following inoculation with Agrobacterium tumefaciens strains EHA 105 and 1065, carrying gus and nptII genes was enhanced with the supplementation of the co-cultivation medium with naringenin. Supplementation of medium with 100mM (strain EHA 105) and 300 mM (strain 1065) naringenin was most effective at enhancing mean (±s.e.m., n=3) GUS activity in leaf explants (20.3± 2.4%, strain EHA; 105; 6.0 ± 0.57%, strain 1065) and nodal segments (16.7 ± 2.4% strain EHA 105; 8.3 ± 0.57% strain 1065) of P. mollissima. In P. giberti and N. tabacum maximum GUS activitywas obtained in leaf and root explants with 100mM naringenin for both strains analysed. Additionally, when naringenin was added to Luria Bertani (LB) medium, both bacterial growth via optical density and colony forming units were higher when compared to control. This is the first report of the use ofnaringenin to enhance gene transfer from Agrobacterium to plants. These findings suggest that naringenin can be used as an alternative to acetosyringone for vir gene induction in Agrobacterium. This approachmay be especially useful in plants that are generally recalcitrant to Agrobacterium-mediated transformation.
publishDate 2004
dc.date.created.none.fl_str_mv 2004-01-10
dc.date.accessioned.none.fl_str_mv 2018-02-24T16:01:01Z
2020-04-15T18:08:45Z
dc.date.available.none.fl_str_mv 2018-02-24T16:01:01Z
2020-04-15T18:08:45Z
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.hasversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.local.spa.fl_str_mv Artículo de revista
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
format http://purl.org/coar/resource_type/c_6501
dc.identifier.none.fl_str_mv http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4922
dc.identifier.issn.none.fl_str_mv 2027-1352
0122-7483
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10554/31943
url http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4922
http://hdl.handle.net/10554/31943
identifier_str_mv 2027-1352
0122-7483
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.uri.none.fl_str_mv http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4922/3797
dc.relation.citationissue.eng.fl_str_mv Universitas Scientiarum; Vol 9, No 1 (2004); 47-57
dc.relation.citationissue.spa.fl_str_mv Universitas Scientiarum; Vol 9, No 1 (2004); 47-57
dc.relation.citationissue.por.fl_str_mv Universitas Scientiarum; Vol 9, No 1 (2004); 47-57
dc.rights.licence.*.fl_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.spa.fl_str_mv PDF
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv null
null
null
dc.publisher.eng.fl_str_mv Pontificia Universidad Javeriana
institution Pontificia Universidad Javeriana
repository.name.fl_str_mv Repositorio Institucional - Pontificia Universidad Javeriana
repository.mail.fl_str_mv repositorio@javeriana.edu.co
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