AMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI)

We molecularly identified the loggerhead turtle Caretta caretta using high-speed PCR amplification and restriction analysis of mitochondrial DNA. We isolated the DNA from blood from juvenile C. caretta from Don Diego beach (Magdalena; n=4), in Islas Del Rosario (Bolívar; n=2) in the Colombian Caribb...

Full description

Autores:
Lancheros-Piliego, Daniel
Hernández Fernández, Javier; Universidad Jorge Tadeo Lozano
Tipo de recurso:
Article of journal
Fecha de publicación:
2013
Institución:
Pontificia Universidad Javeriana
Repositorio:
Repositorio Universidad Javeriana
Idioma:
eng
OAI Identifier:
oai:repository.javeriana.edu.co:10554/30903
Acceso en línea:
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/6082
http://hdl.handle.net/10554/30903
Palabra clave:
Biología Molecular; Taxonomía; biodiversidad
Caretta caretta; High-speed PCR; Cytochrome c oxidase I (COI); AMDAR; Barcode.
Tortuga cabezona Caretta caretta
Rights
openAccess
License
Atribución-NoComercial-SinDerivadas 4.0 Internacional
id JAVERIANA2_a35e052ef6790805ae4f1261a9df0100
oai_identifier_str oai:repository.javeriana.edu.co:10554/30903
network_acronym_str JAVERIANA2
network_name_str Repositorio Universidad Javeriana
repository_id_str
spelling Atribución-NoComercial-SinDerivadas 4.0 Internacionalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Oficina de Investigaciones, Universidad Jorge TAdeo LozanoLancheros-Piliego, DanielHernández Fernández, Javier; Universidad Jorge Tadeo Lozano2018-02-24T15:58:18Z2020-04-15T18:09:15Z2018-02-24T15:58:18Z2020-04-15T18:09:15Z2013-11-22http://revistas.javeriana.edu.co/index.php/scientarium/article/view/608210.11144/Javeriana.SC18-3.apit2027-13520122-7483http://hdl.handle.net/10554/30903We molecularly identified the loggerhead turtle Caretta caretta using high-speed PCR amplification and restriction analysis of mitochondrial DNA. We isolated the DNA from blood from juvenile C. caretta from Don Diego beach (Magdalena; n=4), in Islas Del Rosario (Bolívar; n=2) in the Colombian Caribbean. By using high-speed PCR amplification of mitochondrial cytochrome c oxidase I (COI), we reduced reaction by one third and obtained fragments of 650 base pairs. We analyzed the amplified IOC product using enzymes HindIII, HpyCH4III and MseI and generated an electrophoretic profile, which compared in silico to other sea turtle species sequences, revealed the loggerhead’s specific pattern. We found similarity between 97-99% with C. caretta in five of the BLAST analyzed nucleotide sequences and 92% in another. We generated a bar code for the sampled turtle information and sequences and stored them in the BOLD database. The methodology described for the identification of C. caretta is a fast and inexpensive procedure that minimizes time and improves PCR specificity.PDFapplication/pdftext/htmlengPontificia Universidad Javerianahttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/6082/5486http://revistas.javeriana.edu.co/index.php/scientarium/article/view/6082/6509Universitas Scientiarum; Vol 18, No 3 (2013); 321-330Universitas Scientiarum; Vol 18, No 3 (2013); 321-330Universitas Scientiarum; Vol 18, No 3 (2013); 321-330Biología Molecular; Taxonomía; biodiversidadCaretta caretta; High-speed PCR; Cytochrome c oxidase I (COI); AMDAR; Barcode.Tortuga cabezona Caretta carettaAMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI)Santa Marta y Cartagena2011Sanguíneahttp://purl.org/coar/version/c_970fb48d4fbd8a85Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlePCR; secuenciación, analisis bioinformático10554/30903oai:repository.javeriana.edu.co:10554/309032023-03-28 16:15:29.843Repositorio Institucional - Pontificia Universidad Javerianarepositorio@javeriana.edu.co
dc.title.spa.fl_str_mv AMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI)
title AMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI)
spellingShingle AMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI)
Biología Molecular; Taxonomía; biodiversidad
Caretta caretta; High-speed PCR; Cytochrome c oxidase I (COI); AMDAR; Barcode.
Tortuga cabezona Caretta caretta
title_short AMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI)
title_full AMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI)
title_fullStr AMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI)
title_full_unstemmed AMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI)
title_sort AMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI)
dc.creator.fl_str_mv Lancheros-Piliego, Daniel
Hernández Fernández, Javier; Universidad Jorge Tadeo Lozano
dc.contributor.author.none.fl_str_mv Lancheros-Piliego, Daniel
Hernández Fernández, Javier; Universidad Jorge Tadeo Lozano
dc.contributor.spa.fl_str_mv Oficina de Investigaciones, Universidad Jorge TAdeo Lozano
dc.subject.spa.fl_str_mv Biología Molecular; Taxonomía; biodiversidad
Caretta caretta; High-speed PCR; Cytochrome c oxidase I (COI); AMDAR; Barcode.
Tortuga cabezona Caretta caretta
topic Biología Molecular; Taxonomía; biodiversidad
Caretta caretta; High-speed PCR; Cytochrome c oxidase I (COI); AMDAR; Barcode.
Tortuga cabezona Caretta caretta
description We molecularly identified the loggerhead turtle Caretta caretta using high-speed PCR amplification and restriction analysis of mitochondrial DNA. We isolated the DNA from blood from juvenile C. caretta from Don Diego beach (Magdalena; n=4), in Islas Del Rosario (Bolívar; n=2) in the Colombian Caribbean. By using high-speed PCR amplification of mitochondrial cytochrome c oxidase I (COI), we reduced reaction by one third and obtained fragments of 650 base pairs. We analyzed the amplified IOC product using enzymes HindIII, HpyCH4III and MseI and generated an electrophoretic profile, which compared in silico to other sea turtle species sequences, revealed the loggerhead’s specific pattern. We found similarity between 97-99% with C. caretta in five of the BLAST analyzed nucleotide sequences and 92% in another. We generated a bar code for the sampled turtle information and sequences and stored them in the BOLD database. The methodology described for the identification of C. caretta is a fast and inexpensive procedure that minimizes time and improves PCR specificity.
publishDate 2013
dc.date.created.none.fl_str_mv 2013-11-22
dc.date.accessioned.none.fl_str_mv 2018-02-24T15:58:18Z
2020-04-15T18:09:15Z
dc.date.available.none.fl_str_mv 2018-02-24T15:58:18Z
2020-04-15T18:09:15Z
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.hasversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.local.spa.fl_str_mv Artículo de revista
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.other.none.fl_str_mv PCR; secuenciación, analisis bioinformático
format http://purl.org/coar/resource_type/c_6501
dc.identifier.none.fl_str_mv http://revistas.javeriana.edu.co/index.php/scientarium/article/view/6082
10.11144/Javeriana.SC18-3.apit
dc.identifier.issn.none.fl_str_mv 2027-1352
0122-7483
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10554/30903
url http://revistas.javeriana.edu.co/index.php/scientarium/article/view/6082
http://hdl.handle.net/10554/30903
identifier_str_mv 10.11144/Javeriana.SC18-3.apit
2027-1352
0122-7483
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.uri.none.fl_str_mv http://revistas.javeriana.edu.co/index.php/scientarium/article/view/6082/5486
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/6082/6509
dc.relation.citationissue.eng.fl_str_mv Universitas Scientiarum; Vol 18, No 3 (2013); 321-330
dc.relation.citationissue.spa.fl_str_mv Universitas Scientiarum; Vol 18, No 3 (2013); 321-330
dc.relation.citationissue.por.fl_str_mv Universitas Scientiarum; Vol 18, No 3 (2013); 321-330
dc.rights.licence.*.fl_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.spa.fl_str_mv PDF
dc.format.mimetype.spa.fl_str_mv application/pdf
text/html
dc.coverage.none.fl_str_mv Santa Marta y Cartagena
2011
Sanguínea
dc.publisher.eng.fl_str_mv Pontificia Universidad Javeriana
institution Pontificia Universidad Javeriana
repository.name.fl_str_mv Repositorio Institucional - Pontificia Universidad Javeriana
repository.mail.fl_str_mv repositorio@javeriana.edu.co
_version_ 1814338198759800832