AMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI)
We molecularly identified the loggerhead turtle Caretta caretta using high-speed PCR amplification and restriction analysis of mitochondrial DNA. We isolated the DNA from blood from juvenile C. caretta from Don Diego beach (Magdalena; n=4), in Islas Del Rosario (Bolívar; n=2) in the Colombian Caribb...
- Autores:
-
Lancheros-Piliego, Daniel
Hernández Fernández, Javier; Universidad Jorge Tadeo Lozano
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2013
- Institución:
- Pontificia Universidad Javeriana
- Repositorio:
- Repositorio Universidad Javeriana
- Idioma:
- eng
- OAI Identifier:
- oai:repository.javeriana.edu.co:10554/30903
- Acceso en línea:
- http://revistas.javeriana.edu.co/index.php/scientarium/article/view/6082
http://hdl.handle.net/10554/30903
- Palabra clave:
- Biología Molecular; Taxonomía; biodiversidad
Caretta caretta; High-speed PCR; Cytochrome c oxidase I (COI); AMDAR; Barcode.
Tortuga cabezona Caretta caretta
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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Atribución-NoComercial-SinDerivadas 4.0 Internacionalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Oficina de Investigaciones, Universidad Jorge TAdeo LozanoLancheros-Piliego, DanielHernández Fernández, Javier; Universidad Jorge Tadeo Lozano2018-02-24T15:58:18Z2020-04-15T18:09:15Z2018-02-24T15:58:18Z2020-04-15T18:09:15Z2013-11-22http://revistas.javeriana.edu.co/index.php/scientarium/article/view/608210.11144/Javeriana.SC18-3.apit2027-13520122-7483http://hdl.handle.net/10554/30903We molecularly identified the loggerhead turtle Caretta caretta using high-speed PCR amplification and restriction analysis of mitochondrial DNA. We isolated the DNA from blood from juvenile C. caretta from Don Diego beach (Magdalena; n=4), in Islas Del Rosario (Bolívar; n=2) in the Colombian Caribbean. By using high-speed PCR amplification of mitochondrial cytochrome c oxidase I (COI), we reduced reaction by one third and obtained fragments of 650 base pairs. We analyzed the amplified IOC product using enzymes HindIII, HpyCH4III and MseI and generated an electrophoretic profile, which compared in silico to other sea turtle species sequences, revealed the loggerhead’s specific pattern. We found similarity between 97-99% with C. caretta in five of the BLAST analyzed nucleotide sequences and 92% in another. We generated a bar code for the sampled turtle information and sequences and stored them in the BOLD database. The methodology described for the identification of C. caretta is a fast and inexpensive procedure that minimizes time and improves PCR specificity.PDFapplication/pdftext/htmlengPontificia Universidad Javerianahttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/6082/5486http://revistas.javeriana.edu.co/index.php/scientarium/article/view/6082/6509Universitas Scientiarum; Vol 18, No 3 (2013); 321-330Universitas Scientiarum; Vol 18, No 3 (2013); 321-330Universitas Scientiarum; Vol 18, No 3 (2013); 321-330Biología Molecular; Taxonomía; biodiversidadCaretta caretta; High-speed PCR; Cytochrome c oxidase I (COI); AMDAR; Barcode.Tortuga cabezona Caretta carettaAMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI)Santa Marta y Cartagena2011Sanguíneahttp://purl.org/coar/version/c_970fb48d4fbd8a85Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlePCR; secuenciación, analisis bioinformático10554/30903oai:repository.javeriana.edu.co:10554/309032023-03-28 16:15:29.843Repositorio Institucional - Pontificia Universidad Javerianarepositorio@javeriana.edu.co |
dc.title.spa.fl_str_mv |
AMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI) |
title |
AMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI) |
spellingShingle |
AMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI) Biología Molecular; Taxonomía; biodiversidad Caretta caretta; High-speed PCR; Cytochrome c oxidase I (COI); AMDAR; Barcode. Tortuga cabezona Caretta caretta |
title_short |
AMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI) |
title_full |
AMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI) |
title_fullStr |
AMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI) |
title_full_unstemmed |
AMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI) |
title_sort |
AMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI) |
dc.creator.fl_str_mv |
Lancheros-Piliego, Daniel Hernández Fernández, Javier; Universidad Jorge Tadeo Lozano |
dc.contributor.author.none.fl_str_mv |
Lancheros-Piliego, Daniel Hernández Fernández, Javier; Universidad Jorge Tadeo Lozano |
dc.contributor.spa.fl_str_mv |
Oficina de Investigaciones, Universidad Jorge TAdeo Lozano |
dc.subject.spa.fl_str_mv |
Biología Molecular; Taxonomía; biodiversidad Caretta caretta; High-speed PCR; Cytochrome c oxidase I (COI); AMDAR; Barcode. Tortuga cabezona Caretta caretta |
topic |
Biología Molecular; Taxonomía; biodiversidad Caretta caretta; High-speed PCR; Cytochrome c oxidase I (COI); AMDAR; Barcode. Tortuga cabezona Caretta caretta |
description |
We molecularly identified the loggerhead turtle Caretta caretta using high-speed PCR amplification and restriction analysis of mitochondrial DNA. We isolated the DNA from blood from juvenile C. caretta from Don Diego beach (Magdalena; n=4), in Islas Del Rosario (Bolívar; n=2) in the Colombian Caribbean. By using high-speed PCR amplification of mitochondrial cytochrome c oxidase I (COI), we reduced reaction by one third and obtained fragments of 650 base pairs. We analyzed the amplified IOC product using enzymes HindIII, HpyCH4III and MseI and generated an electrophoretic profile, which compared in silico to other sea turtle species sequences, revealed the loggerhead’s specific pattern. We found similarity between 97-99% with C. caretta in five of the BLAST analyzed nucleotide sequences and 92% in another. We generated a bar code for the sampled turtle information and sequences and stored them in the BOLD database. The methodology described for the identification of C. caretta is a fast and inexpensive procedure that minimizes time and improves PCR specificity. |
publishDate |
2013 |
dc.date.created.none.fl_str_mv |
2013-11-22 |
dc.date.accessioned.none.fl_str_mv |
2018-02-24T15:58:18Z 2020-04-15T18:09:15Z |
dc.date.available.none.fl_str_mv |
2018-02-24T15:58:18Z 2020-04-15T18:09:15Z |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.hasversion.none.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.local.spa.fl_str_mv |
Artículo de revista |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.other.none.fl_str_mv |
PCR; secuenciación, analisis bioinformático |
format |
http://purl.org/coar/resource_type/c_6501 |
dc.identifier.none.fl_str_mv |
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/6082 10.11144/Javeriana.SC18-3.apit |
dc.identifier.issn.none.fl_str_mv |
2027-1352 0122-7483 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10554/30903 |
url |
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/6082 http://hdl.handle.net/10554/30903 |
identifier_str_mv |
10.11144/Javeriana.SC18-3.apit 2027-1352 0122-7483 |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.relation.uri.none.fl_str_mv |
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/6082/5486 http://revistas.javeriana.edu.co/index.php/scientarium/article/view/6082/6509 |
dc.relation.citationissue.eng.fl_str_mv |
Universitas Scientiarum; Vol 18, No 3 (2013); 321-330 |
dc.relation.citationissue.spa.fl_str_mv |
Universitas Scientiarum; Vol 18, No 3 (2013); 321-330 |
dc.relation.citationissue.por.fl_str_mv |
Universitas Scientiarum; Vol 18, No 3 (2013); 321-330 |
dc.rights.licence.*.fl_str_mv |
Atribución-NoComercial-SinDerivadas 4.0 Internacional |
dc.rights.accessrights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.coar.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
rights_invalid_str_mv |
Atribución-NoComercial-SinDerivadas 4.0 Internacional http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.spa.fl_str_mv |
PDF |
dc.format.mimetype.spa.fl_str_mv |
application/pdf text/html |
dc.coverage.none.fl_str_mv |
Santa Marta y Cartagena 2011 Sanguínea |
dc.publisher.eng.fl_str_mv |
Pontificia Universidad Javeriana |
institution |
Pontificia Universidad Javeriana |
repository.name.fl_str_mv |
Repositorio Institucional - Pontificia Universidad Javeriana |
repository.mail.fl_str_mv |
repositorio@javeriana.edu.co |
_version_ |
1814338198759800832 |