ADAPTACIÓN DE LA CEPA MUNANTA DE TRYPANOSOMA CRUZI AL CULTIVO IN VITRO EN CÉLULAS VERO

The importance of obtaining the different stages of Trypanosoma cruzi in order to recognize the antigens involved in the intracellular invasion and replication processes, makes it necessary to adapt these strains to tissue culture, especially considering the high leve! of biological, biochemical and...

Full description

Autores:
Velazco Gamboa, Carolina; Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
Puentes Corredor, Manuel; Instituto de Inmunología - HSJD, Bogotá
Moreno García, Alberto; Instituto de Inmunología - HSJD, Bogotá
Patarroyo Murillo, Manuel Elkin; Instituto de Inmunología - HSJD, Bogotá
Puerta Bula, Concepción; Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
Tipo de recurso:
Article of journal
Fecha de publicación:
2013
Institución:
Pontificia Universidad Javeriana
Repositorio:
Repositorio Universidad Javeriana
Idioma:
eng
OAI Identifier:
oai:repository.javeriana.edu.co:10554/31443
Acceso en línea:
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/5074
http://hdl.handle.net/10554/31443
Palabra clave:
null
Cultivo tisular; tripomastigotes; amastigotes; epimastigotes; Trypanosoma cruzi
null
Rights
openAccess
License
Atribución-NoComercial-SinDerivadas 4.0 Internacional
id JAVERIANA2_8b851ae76828abb50a5b1663e4cd3102
oai_identifier_str oai:repository.javeriana.edu.co:10554/31443
network_acronym_str JAVERIANA2
network_name_str Repositorio Universidad Javeriana
repository_id_str
spelling Atribución-NoComercial-SinDerivadas 4.0 Internacionalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2nullVelazco Gamboa, Carolina; Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, BogotáPuentes Corredor, Manuel; Instituto de Inmunología - HSJD, BogotáMoreno García, Alberto; Instituto de Inmunología - HSJD, BogotáPatarroyo Murillo, Manuel Elkin; Instituto de Inmunología - HSJD, BogotáPuerta Bula, Concepción; Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá2018-02-24T15:59:55Z2020-04-15T18:10:41Z2018-02-24T15:59:55Z2020-04-15T18:10:41Z2013-05-17http://revistas.javeriana.edu.co/index.php/scientarium/article/view/50742027-13520122-7483http://hdl.handle.net/10554/31443The importance of obtaining the different stages of Trypanosoma cruzi in order to recognize the antigens involved in the intracellular invasion and replication processes, makes it necessary to adapt these strains to tissue culture, especially considering the high leve! of biological, biochemical and genetic variation, which is found among the strains and clones ofthe parasite. Within the aforementioned context, in this study, the Colombian strain ofT. cruzi, M un anta, was adapted to tissue culture in Yero Cells, obtaining the principal forms ofthe parasite: trypomastigotes, amastigotes and epimastigotes. lt was observed that the liberation of the trypomastigotes occurred on the seventh day postinfection and the quantity obtained was directly proportional to the number of infecting parasites. On the other hand, the majority of the trypomastigotes observed 48 hours after washing the monolayer five days after infection, were had thick-looking shapes corresponding to the initiation ofthe trasforrnation from trypomastigotes to amastigotes.PDFapplication/pdfengPontificia Universidad Javerianahttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/5074/3914Universitas Scientiarum; Vol 4, No 1 (1997); 83-94Universitas Scientiarum; Vol 4, No 1 (1997); 83-94Universitas Scientiarum; Vol 4, No 1 (1997); 83-94nullCultivo tisular; tripomastigotes; amastigotes; epimastigotes; Trypanosoma cruzinullADAPTACIÓN DE LA CEPA MUNANTA DE TRYPANOSOMA CRUZI AL CULTIVO IN VITRO EN CÉLULAS VEROnullnullnullhttp://purl.org/coar/version/c_970fb48d4fbd8a85Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/article10554/31443oai:repository.javeriana.edu.co:10554/314432023-03-28 16:15:46.382Repositorio Institucional - Pontificia Universidad Javerianarepositorio@javeriana.edu.co
dc.title.spa.fl_str_mv ADAPTACIÓN DE LA CEPA MUNANTA DE TRYPANOSOMA CRUZI AL CULTIVO IN VITRO EN CÉLULAS VERO
title ADAPTACIÓN DE LA CEPA MUNANTA DE TRYPANOSOMA CRUZI AL CULTIVO IN VITRO EN CÉLULAS VERO
spellingShingle ADAPTACIÓN DE LA CEPA MUNANTA DE TRYPANOSOMA CRUZI AL CULTIVO IN VITRO EN CÉLULAS VERO
null
Cultivo tisular; tripomastigotes; amastigotes; epimastigotes; Trypanosoma cruzi
null
title_short ADAPTACIÓN DE LA CEPA MUNANTA DE TRYPANOSOMA CRUZI AL CULTIVO IN VITRO EN CÉLULAS VERO
title_full ADAPTACIÓN DE LA CEPA MUNANTA DE TRYPANOSOMA CRUZI AL CULTIVO IN VITRO EN CÉLULAS VERO
title_fullStr ADAPTACIÓN DE LA CEPA MUNANTA DE TRYPANOSOMA CRUZI AL CULTIVO IN VITRO EN CÉLULAS VERO
title_full_unstemmed ADAPTACIÓN DE LA CEPA MUNANTA DE TRYPANOSOMA CRUZI AL CULTIVO IN VITRO EN CÉLULAS VERO
title_sort ADAPTACIÓN DE LA CEPA MUNANTA DE TRYPANOSOMA CRUZI AL CULTIVO IN VITRO EN CÉLULAS VERO
dc.creator.fl_str_mv Velazco Gamboa, Carolina; Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
Puentes Corredor, Manuel; Instituto de Inmunología - HSJD, Bogotá
Moreno García, Alberto; Instituto de Inmunología - HSJD, Bogotá
Patarroyo Murillo, Manuel Elkin; Instituto de Inmunología - HSJD, Bogotá
Puerta Bula, Concepción; Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
dc.contributor.author.none.fl_str_mv Velazco Gamboa, Carolina; Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
Puentes Corredor, Manuel; Instituto de Inmunología - HSJD, Bogotá
Moreno García, Alberto; Instituto de Inmunología - HSJD, Bogotá
Patarroyo Murillo, Manuel Elkin; Instituto de Inmunología - HSJD, Bogotá
Puerta Bula, Concepción; Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
dc.contributor.none.fl_str_mv null
dc.subject.spa.fl_str_mv null
Cultivo tisular; tripomastigotes; amastigotes; epimastigotes; Trypanosoma cruzi
null
topic null
Cultivo tisular; tripomastigotes; amastigotes; epimastigotes; Trypanosoma cruzi
null
description The importance of obtaining the different stages of Trypanosoma cruzi in order to recognize the antigens involved in the intracellular invasion and replication processes, makes it necessary to adapt these strains to tissue culture, especially considering the high leve! of biological, biochemical and genetic variation, which is found among the strains and clones ofthe parasite. Within the aforementioned context, in this study, the Colombian strain ofT. cruzi, M un anta, was adapted to tissue culture in Yero Cells, obtaining the principal forms ofthe parasite: trypomastigotes, amastigotes and epimastigotes. lt was observed that the liberation of the trypomastigotes occurred on the seventh day postinfection and the quantity obtained was directly proportional to the number of infecting parasites. On the other hand, the majority of the trypomastigotes observed 48 hours after washing the monolayer five days after infection, were had thick-looking shapes corresponding to the initiation ofthe trasforrnation from trypomastigotes to amastigotes.
publishDate 2013
dc.date.created.none.fl_str_mv 2013-05-17
dc.date.accessioned.none.fl_str_mv 2018-02-24T15:59:55Z
2020-04-15T18:10:41Z
dc.date.available.none.fl_str_mv 2018-02-24T15:59:55Z
2020-04-15T18:10:41Z
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.hasversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.local.spa.fl_str_mv Artículo de revista
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
format http://purl.org/coar/resource_type/c_6501
dc.identifier.none.fl_str_mv http://revistas.javeriana.edu.co/index.php/scientarium/article/view/5074
dc.identifier.issn.none.fl_str_mv 2027-1352
0122-7483
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10554/31443
url http://revistas.javeriana.edu.co/index.php/scientarium/article/view/5074
http://hdl.handle.net/10554/31443
identifier_str_mv 2027-1352
0122-7483
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.uri.none.fl_str_mv http://revistas.javeriana.edu.co/index.php/scientarium/article/view/5074/3914
dc.relation.citationissue.eng.fl_str_mv Universitas Scientiarum; Vol 4, No 1 (1997); 83-94
dc.relation.citationissue.spa.fl_str_mv Universitas Scientiarum; Vol 4, No 1 (1997); 83-94
dc.relation.citationissue.por.fl_str_mv Universitas Scientiarum; Vol 4, No 1 (1997); 83-94
dc.rights.licence.*.fl_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.spa.fl_str_mv PDF
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv null
null
null
dc.publisher.eng.fl_str_mv Pontificia Universidad Javeriana
institution Pontificia Universidad Javeriana
repository.name.fl_str_mv Repositorio Institucional - Pontificia Universidad Javeriana
repository.mail.fl_str_mv repositorio@javeriana.edu.co
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