Assessment of placental extracellular vesicles-associated fas ligand and TNF-related apoptosis-inducing ligand in pregnancies complicated by early and late onset preeclampsia

Preeclampsia (PE) is a hypertensive disorder that affects 2–8% of pregnancies and is one of the main causes of fetal, neonatal, and maternal mortality and morbidity worldwide. Although PE etiology and pathophysiology remain unknown, there is evidence that the hyperactivation of maternal immunity cel...

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Autores:
Ayala Ramírez, Paola
Machuca-Acevedo, Catalina
Gámez, Tatiana
Quijano Gómez, Sandra Milena
Barreto Prieto, Alfonso
Silva Herrera, Jaime Luis
Olaya Contreras, Mercedes
García-Robles, Reggie
Rivatrem
Tipo de recurso:
Article of investigation
Fecha de publicación:
2021
Institución:
Pontificia Universidad Javeriana
Repositorio:
Repositorio Universidad Javeriana
Idioma:
OAI Identifier:
oai:repository.javeriana.edu.co:10554/60055
Acceso en línea:
https://www.frontiersin.org/articles/10.3389/fphys.2021.708824/full
http://hdl.handle.net/10554/60055
https://doi.org/10.3389/fphys.2021.708824
Palabra clave:
Pregnancy
Hypertension Disorder Complicating Pregnancy
Exosome (Vesicle)
Apoptosis
Placental Culture
Rights
License
Atribución-NoComercial 4.0 Internacional
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repository_id_str
spelling Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2Ayala Ramírez, PaolaMachuca-Acevedo, CatalinaGámez, TatianaQuijano Gómez, Sandra MilenaBarreto Prieto, AlfonsoSilva Herrera, Jaime LuisOlaya Contreras, MercedesGarcía-Robles, ReggieRivatremPontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana. Grupo de investigación Instituto de Genética HumanaPontificia Universidad Javeriana. Facultad de Medicina. Departamento de PatologíaPontificia Universidad Javeriana. Facultad de Medicina. Hospital Universitario San IgnacioAyala Ramírez, PaolaQuijano Gómez, Sandra MilenaSilva Herrera, Jaime LuisOlaya-C, MercedesGarcía-Robles, ReggieColombia2018Bogotá (Colombia)2022-05-31T17:18:22Z2022-05-31T17:18:22Z2021-07-23https://www.frontiersin.org/articles/10.3389/fphys.2021.708824/full1664-042Xhttp://hdl.handle.net/10554/60055https://doi.org/10.3389/fphys.2021.708824instname:Pontificia Universidad Javerianareponame:Repositorio Institucional - Pontificia Universidad Javerianarepourl:https://repository.javeriana.edu.coPreeclampsia (PE) is a hypertensive disorder that affects 2–8% of pregnancies and is one of the main causes of fetal, neonatal, and maternal mortality and morbidity worldwide. Although PE etiology and pathophysiology remain unknown, there is evidence that the hyperactivation of maternal immunity cells against placental cells triggers trophoblast cell apoptosis and death. It has also been reported that placenta-derived extracellular vesicles (EV) carry Fas ligand (FasL) and Tumor necrosis factor-related apoptosisinducing ligand (TRAIL) and trigger apoptosis in Jurkat T cells. This study aimed to quantify and compare FasL and TRAIL expression in EV derived from cultures of placenta explants from women with PE (early versus late) and women with uncomplicated pregnancies. Also, the study assessed EV capacity to induce apoptosis in Jurkat T cells. The authors isolated EV from placenta explant cultures, quantified FasL and TRAIL using ELISA, and analyzed EV apoptosis-inducing capability by flow cytometry. Results showed increased FasL and TRAIL in EV derived from placenta of women with PE, and increased EV apoptosis-inducing capability in Jurkat T cells. These results offer supporting evidence that EV FasL and TRAIL play a role in the pathophysiology of PE.Q1Q1Mujeres embarazadashttps://orcid.org/0000-0002-0141-1149https://orcid.org/0000-0003-2482-0954https://orcid.org/0000-0001-6822-0374https://orcid.org/0000-0002-6804-0979Revista Internacional - IndexadaA1NOPDFapplication/pdfPregnancyHypertension Disorder Complicating PregnancyExosome (Vesicle)ApoptosisPlacental CultureAssessment of placental extracellular vesicles-associated fas ligand and TNF-related apoptosis-inducing ligand in pregnancies complicated by early and late onset preeclampsiaArtículo de revistahttp://purl.org/coar/resource_type/c_2df8fbb118Frontiers in Physiology12ORIGINALa0318.pdfapplication/pdf763071http://repository.javeriana.edu.co/bitstream/10554/60055/1/a0318.pdfc40f64b905f13370c54f268fd8f9bf46MD51open accessTHUMBNAILa0318.pdf.jpga0318.pdf.jpgIM Thumbnailimage/jpeg12849http://repository.javeriana.edu.co/bitstream/10554/60055/2/a0318.pdf.jpg9c46a42971027c5aaa90359a4742fffaMD52open access10554/60055oai:repository.javeriana.edu.co:10554/600552023-03-09 12:51:44.328Repositorio Institucional - Pontificia Universidad Javerianarepositorio@javeriana.edu.co
dc.title.none.fl_str_mv Assessment of placental extracellular vesicles-associated fas ligand and TNF-related apoptosis-inducing ligand in pregnancies complicated by early and late onset preeclampsia
title Assessment of placental extracellular vesicles-associated fas ligand and TNF-related apoptosis-inducing ligand in pregnancies complicated by early and late onset preeclampsia
spellingShingle Assessment of placental extracellular vesicles-associated fas ligand and TNF-related apoptosis-inducing ligand in pregnancies complicated by early and late onset preeclampsia
Pregnancy
Hypertension Disorder Complicating Pregnancy
Exosome (Vesicle)
Apoptosis
Placental Culture
title_short Assessment of placental extracellular vesicles-associated fas ligand and TNF-related apoptosis-inducing ligand in pregnancies complicated by early and late onset preeclampsia
title_full Assessment of placental extracellular vesicles-associated fas ligand and TNF-related apoptosis-inducing ligand in pregnancies complicated by early and late onset preeclampsia
title_fullStr Assessment of placental extracellular vesicles-associated fas ligand and TNF-related apoptosis-inducing ligand in pregnancies complicated by early and late onset preeclampsia
title_full_unstemmed Assessment of placental extracellular vesicles-associated fas ligand and TNF-related apoptosis-inducing ligand in pregnancies complicated by early and late onset preeclampsia
title_sort Assessment of placental extracellular vesicles-associated fas ligand and TNF-related apoptosis-inducing ligand in pregnancies complicated by early and late onset preeclampsia
dc.creator.fl_str_mv Ayala Ramírez, Paola
Machuca-Acevedo, Catalina
Gámez, Tatiana
Quijano Gómez, Sandra Milena
Barreto Prieto, Alfonso
Silva Herrera, Jaime Luis
Olaya Contreras, Mercedes
García-Robles, Reggie
Rivatrem
dc.contributor.author.none.fl_str_mv Ayala Ramírez, Paola
Machuca-Acevedo, Catalina
Gámez, Tatiana
Quijano Gómez, Sandra Milena
Barreto Prieto, Alfonso
Silva Herrera, Jaime Luis
Olaya Contreras, Mercedes
García-Robles, Reggie
Rivatrem
dc.contributor.corporatename.none.fl_str_mv Pontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana. Grupo de investigación Instituto de Genética Humana
Pontificia Universidad Javeriana. Facultad de Medicina. Departamento de Patología
dc.contributor.corporatename.spa.fl_str_mv Pontificia Universidad Javeriana. Facultad de Medicina. Hospital Universitario San Ignacio
dc.contributor.javerianateacher.none.fl_str_mv Ayala Ramírez, Paola
Quijano Gómez, Sandra Milena
Silva Herrera, Jaime Luis
Olaya-C, Mercedes
García-Robles, Reggie
dc.subject.spa.fl_str_mv Pregnancy
Hypertension Disorder Complicating Pregnancy
Exosome (Vesicle)
Apoptosis
Placental Culture
topic Pregnancy
Hypertension Disorder Complicating Pregnancy
Exosome (Vesicle)
Apoptosis
Placental Culture
description Preeclampsia (PE) is a hypertensive disorder that affects 2–8% of pregnancies and is one of the main causes of fetal, neonatal, and maternal mortality and morbidity worldwide. Although PE etiology and pathophysiology remain unknown, there is evidence that the hyperactivation of maternal immunity cells against placental cells triggers trophoblast cell apoptosis and death. It has also been reported that placenta-derived extracellular vesicles (EV) carry Fas ligand (FasL) and Tumor necrosis factor-related apoptosisinducing ligand (TRAIL) and trigger apoptosis in Jurkat T cells. This study aimed to quantify and compare FasL and TRAIL expression in EV derived from cultures of placenta explants from women with PE (early versus late) and women with uncomplicated pregnancies. Also, the study assessed EV capacity to induce apoptosis in Jurkat T cells. The authors isolated EV from placenta explant cultures, quantified FasL and TRAIL using ELISA, and analyzed EV apoptosis-inducing capability by flow cytometry. Results showed increased FasL and TRAIL in EV derived from placenta of women with PE, and increased EV apoptosis-inducing capability in Jurkat T cells. These results offer supporting evidence that EV FasL and TRAIL play a role in the pathophysiology of PE.
publishDate 2021
dc.date.created.none.fl_str_mv 2021-07-23
dc.date.accessioned.none.fl_str_mv 2022-05-31T17:18:22Z
dc.date.available.none.fl_str_mv 2022-05-31T17:18:22Z
dc.type.local.spa.fl_str_mv Artículo de revista
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
format http://purl.org/coar/resource_type/c_2df8fbb1
dc.identifier.spa.fl_str_mv https://www.frontiersin.org/articles/10.3389/fphys.2021.708824/full
dc.identifier.issn.spa.fl_str_mv 1664-042X
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10554/60055
dc.identifier.doi.spa.fl_str_mv https://doi.org/10.3389/fphys.2021.708824
dc.identifier.instname.spa.fl_str_mv instname:Pontificia Universidad Javeriana
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional - Pontificia Universidad Javeriana
dc.identifier.repourl.spa.fl_str_mv repourl:https://repository.javeriana.edu.co
url https://www.frontiersin.org/articles/10.3389/fphys.2021.708824/full
http://hdl.handle.net/10554/60055
https://doi.org/10.3389/fphys.2021.708824
identifier_str_mv 1664-042X
instname:Pontificia Universidad Javeriana
reponame:Repositorio Institucional - Pontificia Universidad Javeriana
repourl:https://repository.javeriana.edu.co
dc.relation.citationstartpage.spa.fl_str_mv 1
dc.relation.citationendpage.spa.fl_str_mv 8
dc.relation.ispartofjournal.spa.fl_str_mv Frontiers in Physiology
dc.relation.citationvolume.spa.fl_str_mv 12
dc.rights.licence.*.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.coar.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
dc.format.spa.fl_str_mv PDF
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.coverage.spatial.none.fl_str_mv Colombia
dc.coverage.temporal.none.fl_str_mv 2018
dc.coverage.city.none.fl_str_mv Bogotá (Colombia)
institution Pontificia Universidad Javeriana
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