RUNX1 gene expression changes in the placentas of women smokers

The placenta can be affected by environmental factors, such as exposure to cigarette smoke. This exposure in the fetal context is considered a risk factor for the development of short‑term postnatal diseases, such as asthma. Asthma is an inflammatory disease characterized by predominant acquisition...

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Autores:
Bermudez Liscano, Litzy Gisella
Madariaga Perpiñan, Ithzayana
Zuñiga Sandoval, Maria Isabel
Olaya-C., Mercedes
Canas, Alejandra
Rodríguez C., Luz-Stella
Moreno Niño, Olga María
Rojas, Adriana
Tipo de recurso:
Article of journal
Fecha de publicación:
2021
Institución:
Pontificia Universidad Javeriana
Repositorio:
Repositorio Universidad Javeriana
Idioma:
OAI Identifier:
oai:repository.javeriana.edu.co:10554/59918
Acceso en línea:
https://www.spandidos-publications.com/10.3892/etm.2021.10334
http://hdl.handle.net/10554/59918
https://doi.org/10.3892/etm.2021.10334
Palabra clave:
Placenta
Runx1
Gata Binding Protein 3
Transcription Factor
Maternal Exposure
Cigarette Smoking
Rights
License
Atribución-NoComercial 4.0 Internacional
id JAVERIANA2_823ac0b44ec127d0c9e7e6549a212be2
oai_identifier_str oai:repository.javeriana.edu.co:10554/59918
network_acronym_str JAVERIANA2
network_name_str Repositorio Universidad Javeriana
repository_id_str
dc.title.none.fl_str_mv RUNX1 gene expression changes in the placentas of women smokers
title RUNX1 gene expression changes in the placentas of women smokers
spellingShingle RUNX1 gene expression changes in the placentas of women smokers
Placenta
Runx1
Gata Binding Protein 3
Transcription Factor
Maternal Exposure
Cigarette Smoking
title_short RUNX1 gene expression changes in the placentas of women smokers
title_full RUNX1 gene expression changes in the placentas of women smokers
title_fullStr RUNX1 gene expression changes in the placentas of women smokers
title_full_unstemmed RUNX1 gene expression changes in the placentas of women smokers
title_sort RUNX1 gene expression changes in the placentas of women smokers
dc.creator.fl_str_mv Bermudez Liscano, Litzy Gisella
Madariaga Perpiñan, Ithzayana
Zuñiga Sandoval, Maria Isabel
Olaya-C., Mercedes
Canas, Alejandra
Rodríguez C., Luz-Stella
Moreno Niño, Olga María
Rojas, Adriana
dc.contributor.author.none.fl_str_mv Bermudez Liscano, Litzy Gisella
Madariaga Perpiñan, Ithzayana
Zuñiga Sandoval, Maria Isabel
Olaya-C., Mercedes
Canas, Alejandra
Rodríguez C., Luz-Stella
Moreno Niño, Olga María
Rojas, Adriana
dc.contributor.corporatename.spa.fl_str_mv Pontificia Universidad Javeriana. Facultad de Medicina. Departamento de Medicina Interna. Neumología
dc.contributor.corporatename.none.fl_str_mv Pontificia Universidad Javeriana. Facultad de Medicina. Hospital Universitario San Ignacio
Pontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana. Grupo de investigación Instituto de Genética Humana
Pontificia Universidad Javeriana. Facultad de Medicina. Departamento de Patología
dc.contributor.javerianateacher.none.fl_str_mv Olaya-C., Mercedes
Canas, Alejandra
Rodríguez C., Luz-Stella
Moreno Niño, Olga María
Rojas, Adriana
dc.subject.none.fl_str_mv Placenta
Runx1
Gata Binding Protein 3
Transcription Factor
Maternal Exposure
Cigarette Smoking
topic Placenta
Runx1
Gata Binding Protein 3
Transcription Factor
Maternal Exposure
Cigarette Smoking
description The placenta can be affected by environmental factors, such as exposure to cigarette smoke. This exposure in the fetal context is considered a risk factor for the development of short‑term postnatal diseases, such as asthma. Asthma is an inflammatory disease characterized by predominant acquisition of CD4 T lymphocytes (TLs) of the Th2 type. Transcription factors such as GATA binding protein 3 (GATA3) and STAT6 actively participate in the differentiation of virgin TLs towards the Th2 profile, while transcription factors such as STAT1, T‑Box transcription factor 21 (T‑BET), RUNX1 and RUNX3 participate in their differentiation towards the Th1 profile. The objective of the current study was to evaluate the impact of exposure to cigarette smoke on the gene expression of STAT1, T‑BET, GATA3, IL‑4, RUNX1 and RUNX3 during the gestation period, and to determine whether the expression levels of these genes are associated with changes in global methylation. STAT1, GATA3, RUNX1 and RUNX3 protein and mRNA expression levels in the placental tissue of women smokers and non‑smoking women were determined via immunohistochemistry and quantitative PCR (qPCR) respectively. Additionally, T‑BET and IL‑4 mRNA expression levels were determined by qPCR. On the other hand, global methylation was determined via ELISA. In the present study, significant increases were observed in RUNX1 transcription factor expression in placentas from women smokers when compared with placentas of non‑smoking women. Similarly, significant increases in the expression of GATA3, IL‑4 and RUNX3 mRNA were observed. The changes in gene expression were not associated with changes in the global methylation levels. Finally, a higher frequency of low‑birth‑weight infants were identified in cases of exposure to cigarette smoke during pregnancy when compared with infants not exposed to cigarette smoke during pregnancy. Thus, the data of the present study contributed to the understanding of the genetic and clinical impacts of exposure to cigarette smoke during pregnancy and its importance in maternal and fetal health.
publishDate 2021
dc.date.created.none.fl_str_mv 2021-06-24
dc.date.accessioned.none.fl_str_mv 2022-05-31T15:34:08Z
dc.date.available.none.fl_str_mv 2022-05-31T15:34:08Z
dc.type.local.spa.fl_str_mv Artículo de revista
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dc.identifier.none.fl_str_mv https://www.spandidos-publications.com/10.3892/etm.2021.10334
dc.identifier.issn.none.fl_str_mv 1792-0981 / 1792-1015 (Electrónico)
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10554/59918
dc.identifier.doi.none.fl_str_mv https://doi.org/10.3892/etm.2021.10334
dc.identifier.instname.spa.fl_str_mv instname:Pontificia Universidad Javeriana
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional - Pontificia Universidad Javeriana
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url https://www.spandidos-publications.com/10.3892/etm.2021.10334
http://hdl.handle.net/10554/59918
https://doi.org/10.3892/etm.2021.10334
identifier_str_mv 1792-0981 / 1792-1015 (Electrónico)
instname:Pontificia Universidad Javeriana
reponame:Repositorio Institucional - Pontificia Universidad Javeriana
repourl:https://repository.javeriana.edu.co
dc.relation.citationstartpage.none.fl_str_mv 1
dc.relation.citationendpage.none.fl_str_mv 12
dc.relation.ispartofjournal.none.fl_str_mv Experimental and Therapeutic Medicine
dc.relation.citationvolume.none.fl_str_mv 22
dc.relation.citationissue.none.fl_str_mv 2
dc.rights.licence.*.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
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rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
http://creativecommons.org/licenses/by-nc/4.0/
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dc.coverage.spatial.none.fl_str_mv Colombia
dc.coverage.temporal.none.fl_str_mv 2013-2019
dc.coverage.city.none.fl_str_mv Bogotá (Colombia)
institution Pontificia Universidad Javeriana
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spelling Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2Bermudez Liscano, Litzy GisellaMadariaga Perpiñan, IthzayanaZuñiga Sandoval, Maria IsabelOlaya-C., MercedesCanas, AlejandraRodríguez C., Luz-StellaMoreno Niño, Olga MaríaRojas, AdrianaPontificia Universidad Javeriana. Facultad de Medicina. Departamento de Medicina Interna. NeumologíaPontificia Universidad Javeriana. Facultad de Medicina. Hospital Universitario San IgnacioPontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana. Grupo de investigación Instituto de Genética HumanaPontificia Universidad Javeriana. Facultad de Medicina. Departamento de PatologíaOlaya-C., MercedesCanas, AlejandraRodríguez C., Luz-StellaMoreno Niño, Olga MaríaRojas, AdrianaColombia2013-2019Bogotá (Colombia)2022-05-31T15:34:08Z2022-05-31T15:34:08Z2021-06-24https://www.spandidos-publications.com/10.3892/etm.2021.103341792-0981 / 1792-1015 (Electrónico)http://hdl.handle.net/10554/59918https://doi.org/10.3892/etm.2021.10334instname:Pontificia Universidad Javerianareponame:Repositorio Institucional - Pontificia Universidad Javerianarepourl:https://repository.javeriana.edu.coThe placenta can be affected by environmental factors, such as exposure to cigarette smoke. This exposure in the fetal context is considered a risk factor for the development of short‑term postnatal diseases, such as asthma. Asthma is an inflammatory disease characterized by predominant acquisition of CD4 T lymphocytes (TLs) of the Th2 type. Transcription factors such as GATA binding protein 3 (GATA3) and STAT6 actively participate in the differentiation of virgin TLs towards the Th2 profile, while transcription factors such as STAT1, T‑Box transcription factor 21 (T‑BET), RUNX1 and RUNX3 participate in their differentiation towards the Th1 profile. The objective of the current study was to evaluate the impact of exposure to cigarette smoke on the gene expression of STAT1, T‑BET, GATA3, IL‑4, RUNX1 and RUNX3 during the gestation period, and to determine whether the expression levels of these genes are associated with changes in global methylation. STAT1, GATA3, RUNX1 and RUNX3 protein and mRNA expression levels in the placental tissue of women smokers and non‑smoking women were determined via immunohistochemistry and quantitative PCR (qPCR) respectively. Additionally, T‑BET and IL‑4 mRNA expression levels were determined by qPCR. On the other hand, global methylation was determined via ELISA. In the present study, significant increases were observed in RUNX1 transcription factor expression in placentas from women smokers when compared with placentas of non‑smoking women. Similarly, significant increases in the expression of GATA3, IL‑4 and RUNX3 mRNA were observed. The changes in gene expression were not associated with changes in the global methylation levels. Finally, a higher frequency of low‑birth‑weight infants were identified in cases of exposure to cigarette smoke during pregnancy when compared with infants not exposed to cigarette smoke during pregnancy. Thus, the data of the present study contributed to the understanding of the genetic and clinical impacts of exposure to cigarette smoke during pregnancy and its importance in maternal and fetal health.Q4Mujeres embarazadashttps://orcid.org/0000-0002-6563-7067https://orcid.org/0000-0002-5042-9134https://orcid.org/0000-0002-7147-425Xhttps://orcid.org/0000-0003-3975-2835https://orcid.org/0000-0003-4990-5807https://orcid.org/0000-0002-0826-6191https://orcid.org/0000-0001-8528-4433Revista Internacional - IndexadaNoPDFapplication/pdfPlacentaRunx1Gata Binding Protein 3Transcription FactorMaternal ExposureCigarette SmokingRUNX1 gene expression changes in the placentas of women smokersArtículo de revistahttp://purl.org/coar/resource_type/c_6501112Experimental and Therapeutic Medicine222ORIGINALa3580.pdfapplication/pdf5648892http://repository.javeriana.edu.co/bitstream/10554/59918/1/a3580.pdf62cbde79f1a82b562d49066f86d1c770MD51open accessTHUMBNAILa3580.pdf.jpga3580.pdf.jpgIM Thumbnailimage/jpeg9665http://repository.javeriana.edu.co/bitstream/10554/59918/2/a3580.pdf.jpg8894177590b841999802092a49430565MD52open access10554/59918oai:repository.javeriana.edu.co:10554/599182023-05-18 16:18:52.303Repositorio Institucional - Pontificia Universidad Javerianarepositorio@javeriana.edu.co