RUNX1 gene expression changes in the placentas of women smokers
The placenta can be affected by environmental factors, such as exposure to cigarette smoke. This exposure in the fetal context is considered a risk factor for the development of short‑term postnatal diseases, such as asthma. Asthma is an inflammatory disease characterized by predominant acquisition...
- Autores:
-
Bermudez Liscano, Litzy Gisella
Madariaga Perpiñan, Ithzayana
Zuñiga Sandoval, Maria Isabel
Olaya-C., Mercedes
Canas, Alejandra
Rodríguez C., Luz-Stella
Moreno Niño, Olga María
Rojas, Adriana
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2021
- Institución:
- Pontificia Universidad Javeriana
- Repositorio:
- Repositorio Universidad Javeriana
- Idioma:
- OAI Identifier:
- oai:repository.javeriana.edu.co:10554/59918
- Acceso en línea:
- https://www.spandidos-publications.com/10.3892/etm.2021.10334
http://hdl.handle.net/10554/59918
https://doi.org/10.3892/etm.2021.10334
- Palabra clave:
- Placenta
Runx1
Gata Binding Protein 3
Transcription Factor
Maternal Exposure
Cigarette Smoking
- Rights
- License
- Atribución-NoComercial 4.0 Internacional
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|
dc.title.none.fl_str_mv |
RUNX1 gene expression changes in the placentas of women smokers |
title |
RUNX1 gene expression changes in the placentas of women smokers |
spellingShingle |
RUNX1 gene expression changes in the placentas of women smokers Placenta Runx1 Gata Binding Protein 3 Transcription Factor Maternal Exposure Cigarette Smoking |
title_short |
RUNX1 gene expression changes in the placentas of women smokers |
title_full |
RUNX1 gene expression changes in the placentas of women smokers |
title_fullStr |
RUNX1 gene expression changes in the placentas of women smokers |
title_full_unstemmed |
RUNX1 gene expression changes in the placentas of women smokers |
title_sort |
RUNX1 gene expression changes in the placentas of women smokers |
dc.creator.fl_str_mv |
Bermudez Liscano, Litzy Gisella Madariaga Perpiñan, Ithzayana Zuñiga Sandoval, Maria Isabel Olaya-C., Mercedes Canas, Alejandra Rodríguez C., Luz-Stella Moreno Niño, Olga María Rojas, Adriana |
dc.contributor.author.none.fl_str_mv |
Bermudez Liscano, Litzy Gisella Madariaga Perpiñan, Ithzayana Zuñiga Sandoval, Maria Isabel Olaya-C., Mercedes Canas, Alejandra Rodríguez C., Luz-Stella Moreno Niño, Olga María Rojas, Adriana |
dc.contributor.corporatename.spa.fl_str_mv |
Pontificia Universidad Javeriana. Facultad de Medicina. Departamento de Medicina Interna. Neumología |
dc.contributor.corporatename.none.fl_str_mv |
Pontificia Universidad Javeriana. Facultad de Medicina. Hospital Universitario San Ignacio Pontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana. Grupo de investigación Instituto de Genética Humana Pontificia Universidad Javeriana. Facultad de Medicina. Departamento de Patología |
dc.contributor.javerianateacher.none.fl_str_mv |
Olaya-C., Mercedes Canas, Alejandra Rodríguez C., Luz-Stella Moreno Niño, Olga María Rojas, Adriana |
dc.subject.none.fl_str_mv |
Placenta Runx1 Gata Binding Protein 3 Transcription Factor Maternal Exposure Cigarette Smoking |
topic |
Placenta Runx1 Gata Binding Protein 3 Transcription Factor Maternal Exposure Cigarette Smoking |
description |
The placenta can be affected by environmental factors, such as exposure to cigarette smoke. This exposure in the fetal context is considered a risk factor for the development of short‑term postnatal diseases, such as asthma. Asthma is an inflammatory disease characterized by predominant acquisition of CD4 T lymphocytes (TLs) of the Th2 type. Transcription factors such as GATA binding protein 3 (GATA3) and STAT6 actively participate in the differentiation of virgin TLs towards the Th2 profile, while transcription factors such as STAT1, T‑Box transcription factor 21 (T‑BET), RUNX1 and RUNX3 participate in their differentiation towards the Th1 profile. The objective of the current study was to evaluate the impact of exposure to cigarette smoke on the gene expression of STAT1, T‑BET, GATA3, IL‑4, RUNX1 and RUNX3 during the gestation period, and to determine whether the expression levels of these genes are associated with changes in global methylation. STAT1, GATA3, RUNX1 and RUNX3 protein and mRNA expression levels in the placental tissue of women smokers and non‑smoking women were determined via immunohistochemistry and quantitative PCR (qPCR) respectively. Additionally, T‑BET and IL‑4 mRNA expression levels were determined by qPCR. On the other hand, global methylation was determined via ELISA. In the present study, significant increases were observed in RUNX1 transcription factor expression in placentas from women smokers when compared with placentas of non‑smoking women. Similarly, significant increases in the expression of GATA3, IL‑4 and RUNX3 mRNA were observed. The changes in gene expression were not associated with changes in the global methylation levels. Finally, a higher frequency of low‑birth‑weight infants were identified in cases of exposure to cigarette smoke during pregnancy when compared with infants not exposed to cigarette smoke during pregnancy. Thus, the data of the present study contributed to the understanding of the genetic and clinical impacts of exposure to cigarette smoke during pregnancy and its importance in maternal and fetal health. |
publishDate |
2021 |
dc.date.created.none.fl_str_mv |
2021-06-24 |
dc.date.accessioned.none.fl_str_mv |
2022-05-31T15:34:08Z |
dc.date.available.none.fl_str_mv |
2022-05-31T15:34:08Z |
dc.type.local.spa.fl_str_mv |
Artículo de revista |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
format |
http://purl.org/coar/resource_type/c_6501 |
dc.identifier.none.fl_str_mv |
https://www.spandidos-publications.com/10.3892/etm.2021.10334 |
dc.identifier.issn.none.fl_str_mv |
1792-0981 / 1792-1015 (Electrónico) |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10554/59918 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.3892/etm.2021.10334 |
dc.identifier.instname.spa.fl_str_mv |
instname:Pontificia Universidad Javeriana |
dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional - Pontificia Universidad Javeriana |
dc.identifier.repourl.spa.fl_str_mv |
repourl:https://repository.javeriana.edu.co |
url |
https://www.spandidos-publications.com/10.3892/etm.2021.10334 http://hdl.handle.net/10554/59918 https://doi.org/10.3892/etm.2021.10334 |
identifier_str_mv |
1792-0981 / 1792-1015 (Electrónico) instname:Pontificia Universidad Javeriana reponame:Repositorio Institucional - Pontificia Universidad Javeriana repourl:https://repository.javeriana.edu.co |
dc.relation.citationstartpage.none.fl_str_mv |
1 |
dc.relation.citationendpage.none.fl_str_mv |
12 |
dc.relation.ispartofjournal.none.fl_str_mv |
Experimental and Therapeutic Medicine |
dc.relation.citationvolume.none.fl_str_mv |
22 |
dc.relation.citationissue.none.fl_str_mv |
2 |
dc.rights.licence.*.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
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http://purl.org/coar/access_right/c_abf2 |
rights_invalid_str_mv |
Atribución-NoComercial 4.0 Internacional http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
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PDF |
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application/pdf |
dc.coverage.spatial.none.fl_str_mv |
Colombia |
dc.coverage.temporal.none.fl_str_mv |
2013-2019 |
dc.coverage.city.none.fl_str_mv |
Bogotá (Colombia) |
institution |
Pontificia Universidad Javeriana |
bitstream.url.fl_str_mv |
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Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2Bermudez Liscano, Litzy GisellaMadariaga Perpiñan, IthzayanaZuñiga Sandoval, Maria IsabelOlaya-C., MercedesCanas, AlejandraRodríguez C., Luz-StellaMoreno Niño, Olga MaríaRojas, AdrianaPontificia Universidad Javeriana. Facultad de Medicina. Departamento de Medicina Interna. NeumologíaPontificia Universidad Javeriana. Facultad de Medicina. Hospital Universitario San IgnacioPontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana. Grupo de investigación Instituto de Genética HumanaPontificia Universidad Javeriana. Facultad de Medicina. Departamento de PatologíaOlaya-C., MercedesCanas, AlejandraRodríguez C., Luz-StellaMoreno Niño, Olga MaríaRojas, AdrianaColombia2013-2019Bogotá (Colombia)2022-05-31T15:34:08Z2022-05-31T15:34:08Z2021-06-24https://www.spandidos-publications.com/10.3892/etm.2021.103341792-0981 / 1792-1015 (Electrónico)http://hdl.handle.net/10554/59918https://doi.org/10.3892/etm.2021.10334instname:Pontificia Universidad Javerianareponame:Repositorio Institucional - Pontificia Universidad Javerianarepourl:https://repository.javeriana.edu.coThe placenta can be affected by environmental factors, such as exposure to cigarette smoke. This exposure in the fetal context is considered a risk factor for the development of short‑term postnatal diseases, such as asthma. Asthma is an inflammatory disease characterized by predominant acquisition of CD4 T lymphocytes (TLs) of the Th2 type. Transcription factors such as GATA binding protein 3 (GATA3) and STAT6 actively participate in the differentiation of virgin TLs towards the Th2 profile, while transcription factors such as STAT1, T‑Box transcription factor 21 (T‑BET), RUNX1 and RUNX3 participate in their differentiation towards the Th1 profile. The objective of the current study was to evaluate the impact of exposure to cigarette smoke on the gene expression of STAT1, T‑BET, GATA3, IL‑4, RUNX1 and RUNX3 during the gestation period, and to determine whether the expression levels of these genes are associated with changes in global methylation. STAT1, GATA3, RUNX1 and RUNX3 protein and mRNA expression levels in the placental tissue of women smokers and non‑smoking women were determined via immunohistochemistry and quantitative PCR (qPCR) respectively. Additionally, T‑BET and IL‑4 mRNA expression levels were determined by qPCR. On the other hand, global methylation was determined via ELISA. In the present study, significant increases were observed in RUNX1 transcription factor expression in placentas from women smokers when compared with placentas of non‑smoking women. Similarly, significant increases in the expression of GATA3, IL‑4 and RUNX3 mRNA were observed. The changes in gene expression were not associated with changes in the global methylation levels. Finally, a higher frequency of low‑birth‑weight infants were identified in cases of exposure to cigarette smoke during pregnancy when compared with infants not exposed to cigarette smoke during pregnancy. Thus, the data of the present study contributed to the understanding of the genetic and clinical impacts of exposure to cigarette smoke during pregnancy and its importance in maternal and fetal health.Q4Mujeres embarazadashttps://orcid.org/0000-0002-6563-7067https://orcid.org/0000-0002-5042-9134https://orcid.org/0000-0002-7147-425Xhttps://orcid.org/0000-0003-3975-2835https://orcid.org/0000-0003-4990-5807https://orcid.org/0000-0002-0826-6191https://orcid.org/0000-0001-8528-4433Revista Internacional - IndexadaNoPDFapplication/pdfPlacentaRunx1Gata Binding Protein 3Transcription FactorMaternal ExposureCigarette SmokingRUNX1 gene expression changes in the placentas of women smokersArtículo de revistahttp://purl.org/coar/resource_type/c_6501112Experimental and Therapeutic Medicine222ORIGINALa3580.pdfapplication/pdf5648892http://repository.javeriana.edu.co/bitstream/10554/59918/1/a3580.pdf62cbde79f1a82b562d49066f86d1c770MD51open accessTHUMBNAILa3580.pdf.jpga3580.pdf.jpgIM Thumbnailimage/jpeg9665http://repository.javeriana.edu.co/bitstream/10554/59918/2/a3580.pdf.jpg8894177590b841999802092a49430565MD52open access10554/59918oai:repository.javeriana.edu.co:10554/599182023-05-18 16:18:52.303Repositorio Institucional - Pontificia Universidad Javerianarepositorio@javeriana.edu.co |