Transcriptional regulation of CDKN2A/p16 by sirtuin 7 in senescence
Q3
- Autores:
-
Rodríguez, Sergio
Bermudez Liscano, Litzy Gisella
González Cubides, Daniel Mauricio
Bernal, Camila
Canas, Alejandra
Morales-Ruíz, Teresa
Henríquez, Berta
Rojas, Adriana
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2022
- Institución:
- Pontificia Universidad Javeriana
- Repositorio:
- Repositorio Universidad Javeriana
- Idioma:
- eng
- OAI Identifier:
- oai:repository.javeriana.edu.co:10554/62151
- Acceso en línea:
- https://www.spandidos-publications.com/10.3892/mmr.2022.12861
http://hdl.handle.net/10554/62151
https://doi.org/10.3892/mmr.2022.12861
- Palabra clave:
- Senescence
Sirtuin 7
Cyclin‑dependent kinase inhibitor 2A/p16
Aging
Epigenetics
- Rights
- License
- Atribución-NoComercial 4.0 Internacional
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Repositorio Universidad Javeriana |
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| dc.title.spa.fl_str_mv |
Transcriptional regulation of CDKN2A/p16 by sirtuin 7 in senescence |
| title |
Transcriptional regulation of CDKN2A/p16 by sirtuin 7 in senescence |
| spellingShingle |
Transcriptional regulation of CDKN2A/p16 by sirtuin 7 in senescence Senescence Sirtuin 7 Cyclin‑dependent kinase inhibitor 2A/p16 Aging Epigenetics |
| title_short |
Transcriptional regulation of CDKN2A/p16 by sirtuin 7 in senescence |
| title_full |
Transcriptional regulation of CDKN2A/p16 by sirtuin 7 in senescence |
| title_fullStr |
Transcriptional regulation of CDKN2A/p16 by sirtuin 7 in senescence |
| title_full_unstemmed |
Transcriptional regulation of CDKN2A/p16 by sirtuin 7 in senescence |
| title_sort |
Transcriptional regulation of CDKN2A/p16 by sirtuin 7 in senescence |
| dc.creator.fl_str_mv |
Rodríguez, Sergio Bermudez Liscano, Litzy Gisella González Cubides, Daniel Mauricio Bernal, Camila Canas, Alejandra Morales-Ruíz, Teresa Henríquez, Berta Rojas, Adriana |
| dc.contributor.author.none.fl_str_mv |
Rodríguez, Sergio Bermudez Liscano, Litzy Gisella González Cubides, Daniel Mauricio Bernal, Camila Canas, Alejandra Morales-Ruíz, Teresa Henríquez, Berta Rojas, Adriana |
| dc.contributor.corporatename.spa.fl_str_mv |
Pontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana. Grupo de investigación Instituto de Genética Humana |
| dc.contributor.corporatename.none.fl_str_mv |
Pontificia Universidad Javeriana. Facultad de Medicina. Departamento de Medicina Interna. Neumología |
| dc.contributor.javerianateacher.none.fl_str_mv |
Canas, Alejandra Rojas, Adriana |
| dc.subject.keyword.spa.fl_str_mv |
Senescence Sirtuin 7 Cyclin‑dependent kinase inhibitor 2A/p16 Aging Epigenetics |
| topic |
Senescence Sirtuin 7 Cyclin‑dependent kinase inhibitor 2A/p16 Aging Epigenetics |
| description |
Q3 |
| publishDate |
2022 |
| dc.date.accessioned.none.fl_str_mv |
2022-10-24T11:30:37Z |
| dc.date.available.none.fl_str_mv |
2022-10-24T11:30:37Z |
| dc.date.created.none.fl_str_mv |
2022-09-27 |
| dc.type.local.spa.fl_str_mv |
Artículo de revista |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/coar/resource_type/c_6501 |
| dc.identifier.spa.fl_str_mv |
https://www.spandidos-publications.com/10.3892/mmr.2022.12861 |
| dc.identifier.issn.spa.fl_str_mv |
1791-2997 / 1791-3004 (Electrónico) |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10554/62151 |
| dc.identifier.doi.spa.fl_str_mv |
https://doi.org/10.3892/mmr.2022.12861 |
| dc.identifier.instname.spa.fl_str_mv |
instname:Pontificia Universidad Javeriana |
| dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional - Pontificia Universidad Javeriana |
| dc.identifier.repourl.spa.fl_str_mv |
repourl:https://repository.javeriana.edu.co |
| url |
https://www.spandidos-publications.com/10.3892/mmr.2022.12861 http://hdl.handle.net/10554/62151 https://doi.org/10.3892/mmr.2022.12861 |
| identifier_str_mv |
1791-2997 / 1791-3004 (Electrónico) instname:Pontificia Universidad Javeriana reponame:Repositorio Institucional - Pontificia Universidad Javeriana repourl:https://repository.javeriana.edu.co |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
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1 |
| dc.relation.citationendpage.spa.fl_str_mv |
9 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Molecular Medicine Reports |
| dc.relation.citationvolume.spa.fl_str_mv |
26 |
| dc.relation.citationissue.spa.fl_str_mv |
5 |
| dc.rights.licence.*.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
| dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
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http://purl.org/coar/access_right/c_abf2 |
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Atribución-NoComercial 4.0 Internacional http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
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PDF |
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Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2Rodríguez, SergioBermudez Liscano, Litzy GisellaGonzález Cubides, Daniel MauricioBernal, CamilaCanas, AlejandraMorales-Ruíz, TeresaHenríquez, BertaRojas, AdrianaPontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana. Grupo de investigación Instituto de Genética HumanaPontificia Universidad Javeriana. Facultad de Medicina. Departamento de Medicina Interna. NeumologíaCanas, AlejandraRojas, Adriana2022-10-24T11:30:37Z2022-10-24T11:30:37Z2022-09-27https://www.spandidos-publications.com/10.3892/mmr.2022.128611791-2997 / 1791-3004 (Electrónico)http://hdl.handle.net/10554/62151https://doi.org/10.3892/mmr.2022.12861instname:Pontificia Universidad Javerianareponame:Repositorio Institucional - Pontificia Universidad Javerianarepourl:https://repository.javeriana.edu.coPDFapplication/pdfengTranscriptional regulation of CDKN2A/p16 by sirtuin 7 in senescenceArtículo de revistahttp://purl.org/coar/resource_type/c_6501Q3Q3Cell senescence is a state of limited cell prolif‑ eration during a stress response or as part of a programmed process. When a senescent cell stops dividing, maintaining metabolic activity contributes to cellular homeostasis mainte‑ nance. In this process, the cell cycle is arrested at the G0/G1 phase. p16INK4A protein is a key regulator of this process via its cyclin‑dependent kinase inhibitor (CDKI) function. CDKI 2A (CDKN2A)/p16 gene expression is regulated by DNA methylation and histone acetylation. Sirtuins (SIRTs) are nicotinamide dinucleotide (NAD+ )‑dependent deacetylases that have properties which prevent diseases and reverse certain aspects of aging (such as immune, metabolic and cardiovas‑ cular diseases). By performing quantitative PCR, Western blot, ChIP, and siRNAs assays, in this study it was demon‑ strated that CDKN2A/p16 gene transcriptional activation and repression were accompanied by selective deposition and elimination of histone acetylation during the senescence of MRC5 cells. Specifically, significant H3K9Ac and H3K18Ac enrichment in cells with a senescent phenotype concomitant with CDKN2A/p16 gene overexpression was demonstrated compared with the non‑senescent phenotype. Furthermore, the presence of H3K18Ac in deacetyl‑transferase SIRT7 knockdown MRC5 cells allowed CDKN2A/p16 promoter activation. These results suggested that SIRT7 served as a critical component of an epigenetic mechanism involved in senescence mediated by the CDKN2A/p16 gene.https://orcid.org/0000-0002-6563-7067https://orcid.org/0000-0001-8225-4394https://orcid.org/0000-0003-3975-2835https://orcid.org/0000-0001-8528-4433Revista Internacional - IndexadaBNoSenescenceSirtuin 7Cyclin‑dependent kinase inhibitor 2A/p16AgingEpigenetics19Molecular Medicine Reports265ORIGINALTranscriptional regulation of CDKN2Ap16.pdfTranscriptional regulation of CDKN2Ap16.pdfArtículoapplication/pdf2754267http://repository.javeriana.edu.co/bitstream/10554/62151/1/Transcriptional%20regulation%20of%20CDKN2Ap16.pdf899ae98ba7f9aebadc03b5fde5bc850cMD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-82603http://repository.javeriana.edu.co/bitstream/10554/62151/2/license.txt2070d280cc89439d983d9eee1b17df53MD52open accessTHUMBNAILTranscriptional regulation of CDKN2Ap16.pdf.jpgTranscriptional regulation of CDKN2Ap16.pdf.jpgIM Thumbnailimage/jpeg8820http://repository.javeriana.edu.co/bitstream/10554/62151/4/Transcriptional%20regulation%20of%20CDKN2Ap16.pdf.jpg78f08ab509ca0e0e10d1b8d1d938a09cMD54open access10554/62151oai:repository.javeriana.edu.co:10554/621512023-06-08 11:19:07.184Repositorio Institucional - Pontificia Universidad Javerianarepositorio@javeriana.edu.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 |