ANÁLISIS GENÓMICO DE PARVOVIRUS CANINO POR PCR - RFLP A PARTIR DE AISLAMIENTOS DE CASOS CLÍNICOS SINTOMÁTICOS TOMADOS EN BOGOTÁ, COLOMBIA. ESTUDIO PRELIMINAR
With the objective of detemúning the genotypes of canine parvovirus (CPV) circulating in Bogotá, 56 fecal and blood samples were obtained from younger dogs, up to a year old with hemorrhagic gastroenteritis for its analysis by the polymerase chain reaction - fingerprints (PCR - RFLP) assay. DNA frag...
- Autores:
-
Castillo, Ángela; Grupo de enfermedades infecciosas, Microbiología Agrícola y Veterinaria, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
Díez, Hugo; Grupo de enfermedades infecciosas, Microbiología Agrícola y Veterinaria, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
Almanza, Jorge; Grupo de enfermedades infecciosas, Microbiología Agrícola y Veterinaria, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
Jerabek, Lois; Grupo de enfermedades infecciosas, Microbiología Agrícola y Veterinaria, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
Torres, Orlando; Grupo de enfermedades infecciosas, Microbiología Agrícola y Veterinaria, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2013
- Institución:
- Pontificia Universidad Javeriana
- Repositorio:
- Repositorio Universidad Javeriana
- Idioma:
- eng
- OAI Identifier:
- oai:repository.javeriana.edu.co:10554/31540
- Acceso en línea:
- http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4838
http://hdl.handle.net/10554/31540
- Palabra clave:
- null
CV-2b and the differentiation of CPV-2 from CPV-2a and CPV-2b. Keywords: Canine parvovirus; polirnerase chain recrian; fingerprints; genotype.
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- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
| Summary: | With the objective of detemúning the genotypes of canine parvovirus (CPV) circulating in Bogotá, 56 fecal and blood samples were obtained from younger dogs, up to a year old with hemorrhagic gastroenteritis for its analysis by the polymerase chain reaction - fingerprints (PCR - RFLP) assay. DNA fragments were obtained from (2,2 Kb) a representative vaccine strain of parvovirus type -2 (CPV-2) and from the four fecal samples were amplified by the PCR assay; they were not obtained from the amplification of the blood samples. The amplified products were digested by the restriction enzyme Rsa I, pemútting the identification of genotypes PCV-2a and PCV-2b and the differentiation of CPV-2 from CPV-2a and CPV-2b. |
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