Electroforesis en Gel de Campo Pulsado (PFGE) para la diferenciación molecular de Listeria monocytogenes

The reporting of L. monocytogenes in food in Colombia is not a mandatory; however, foods considered high-risk are monitored, and the organism is only reported clinically as Gram-positive when it causes meningitis. L. monocytogenes is a foodborne, intracellular, pathogen which causes listeriosis, a disease lethal to humans and animals. Outbreaks of this disease worldwide can bring about human and economic losses. Only a few studies in Colombia have been able to identify and molecularly serotype isolates allowing only the theoretical distribution of serotypes by lineage. This review explains the characteristics of the pathogen, its importance in public health and in the food industry, and provides an overview of PFGE-CHEF; identifying the standard work protocol and the appropriate restriction enzymes to cut DNA. We found that the enzyme combination, Xba- Asc. followed by Apa. offers the best results to differentiate isolates, by grouping them by lineages, and displaying intra-serotype variations. Additionally, we found that in several Latin American countries the results are analyzed using PulseNet; this ensures the comparison of PFGE patterns in equivalent conditions.

• Autores: Cardozo-Bernal, Ángela María; Laboratorio de Biotecnología Molecular, Grupo de Biotecnología Ambiental e Industrial (GBAI). Departamento de Microbiología, Facultad de Ciencias. Pontificia Universidad Javeriana, Bogotá.; Poutou-Piñales, Raúl Alberto; Laboratorio de Biotecnología Molecular, Grupo de Biotecnología Ambiental e Industrial (GBAI). Departamento de Microbiología, Facultad de Ciencias. Pontificia Universidad Javeriana, Bogotá.; Carrascal-Camacho, Ana K.; Laboratorio de Microbiología de Alimentos, Grupo de Biotecnología Ambiental e Industrial (GBAI). Departamento de Microbiología, Facultad de Ciencias. Pontificia Universidad Javeriana, Bogotá.; Ramón-Rodríguez, Luisa Fernanda; Laboratorio de Microbiología de Alimentos, Grupo de Biotecnología Ambiental e Industrial (GBAI). Departamento de Microbiología, Facultad de Ciencias. Pontificia Universidad Javeriana, Bogotá.; Zambrano, Diana Corina; Director área de Investigación y Transferencia de Tecnología. Asociación Colombiana de Porcicultores. FNP-CENIPORCINO, Bogotá, D.C., Colombia
• Tipo de recurso: Article of journal
• Fecha de publicación: 2013
• Institución: Pontificia Universidad Javeriana
• Repositorio: Repositorio Universidad Javeriana
• Idioma: eng
• OAI Identifier: oai:repository.javeriana.edu.co:10554/31067
• Acceso en línea: http://revistas.javeriana.edu.co/index.php/scientarium/article/view/5971; http://hdl.handle.net/10554/31067
• Palabra clave: Microbiology; Molecular typing; Lineages; Serotypes; Listeria; null
• Rights: openAccess
• License: Atribución-NoComercial-SinDerivadas 4.0 Internacional