BORRARGeneration of Organotypic Multicellular Spheres by Magnetic Levitation : Model for the Study of Human Hematopoietic Stem Cells Microenvironment
Q4
- Autores:
-
Mejía-Cruz, Claudia Camila
Barreto-Durán, Emilia
Pardo-Pérez, María Alejandra
Jimenez, María Camila
Rincón, Julieth
Vanegas, Karen
Rodríguez, Jorge Luis
Jaramillo-Garcia, Luis Fernando
Ulloa, Juan Carlos
Martínez Díaz, Rodolfo
Leal-García, Efrain
Pérez-Núñez, Rafael
Barreto, Alfonso
Rodríguez-Pardo, Viviana M
- Tipo de recurso:
- Article of data
- Fecha de publicación:
- 2019
- Institución:
- Pontificia Universidad Javeriana
- Repositorio:
- Repositorio Universidad Javeriana
- Idioma:
- eng
- OAI Identifier:
- oai:repository.javeriana.edu.co:10554/54029
- Acceso en línea:
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457696/
http://hdl.handle.net/10554/54029
https://dx.doi.org/10.15283%2Fijsc18061
- Palabra clave:
- Organotypic multicellular spheres
Organoids
Hematopoietic stem cells
Mesenchymal stem cells
Microenvironment
- Rights
- License
- Atribución-NoComercial 4.0 Internacional
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| dc.title.spa.fl_str_mv |
BORRARGeneration of Organotypic Multicellular Spheres by Magnetic Levitation : Model for the Study of Human Hematopoietic Stem Cells Microenvironment |
| title |
BORRARGeneration of Organotypic Multicellular Spheres by Magnetic Levitation : Model for the Study of Human Hematopoietic Stem Cells Microenvironment |
| spellingShingle |
BORRARGeneration of Organotypic Multicellular Spheres by Magnetic Levitation : Model for the Study of Human Hematopoietic Stem Cells Microenvironment Organotypic multicellular spheres Organoids Hematopoietic stem cells Mesenchymal stem cells Microenvironment |
| title_short |
BORRARGeneration of Organotypic Multicellular Spheres by Magnetic Levitation : Model for the Study of Human Hematopoietic Stem Cells Microenvironment |
| title_full |
BORRARGeneration of Organotypic Multicellular Spheres by Magnetic Levitation : Model for the Study of Human Hematopoietic Stem Cells Microenvironment |
| title_fullStr |
BORRARGeneration of Organotypic Multicellular Spheres by Magnetic Levitation : Model for the Study of Human Hematopoietic Stem Cells Microenvironment |
| title_full_unstemmed |
BORRARGeneration of Organotypic Multicellular Spheres by Magnetic Levitation : Model for the Study of Human Hematopoietic Stem Cells Microenvironment |
| title_sort |
BORRARGeneration of Organotypic Multicellular Spheres by Magnetic Levitation : Model for the Study of Human Hematopoietic Stem Cells Microenvironment |
| dc.creator.fl_str_mv |
Mejía-Cruz, Claudia Camila Barreto-Durán, Emilia Pardo-Pérez, María Alejandra Jimenez, María Camila Rincón, Julieth Vanegas, Karen Rodríguez, Jorge Luis Jaramillo-Garcia, Luis Fernando Ulloa, Juan Carlos Martínez Díaz, Rodolfo Leal-García, Efrain Pérez-Núñez, Rafael Barreto, Alfonso Rodríguez-Pardo, Viviana M |
| dc.contributor.author.none.fl_str_mv |
Mejía-Cruz, Claudia Camila Barreto-Durán, Emilia Pardo-Pérez, María Alejandra Jimenez, María Camila Rincón, Julieth Vanegas, Karen Rodríguez, Jorge Luis Jaramillo-Garcia, Luis Fernando Ulloa, Juan Carlos Martínez Díaz, Rodolfo Leal-García, Efrain Pérez-Núñez, Rafael Barreto, Alfonso Rodríguez-Pardo, Viviana M |
| dc.contributor.corporatename.spa.fl_str_mv |
Pontificia Universidad Javeriana. Facultad de Medicina. Departamento de Ginecología y Obstetricia |
| dc.subject.keyword.spa.fl_str_mv |
Organotypic multicellular spheres Organoids Hematopoietic stem cells Mesenchymal stem cells Microenvironment |
| topic |
Organotypic multicellular spheres Organoids Hematopoietic stem cells Mesenchymal stem cells Microenvironment |
| description |
Q4 |
| publishDate |
2019 |
| dc.date.created.none.fl_str_mv |
2019 |
| dc.date.accessioned.none.fl_str_mv |
2021-06-10T00:20:31Z |
| dc.date.available.none.fl_str_mv |
2021-06-10T00:20:31Z |
| dc.type.local.spa.fl_str_mv |
Artículo de revista |
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http://purl.org/coar/resource_type/c_beb9 |
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http://purl.org/coar/resource_type/c_beb9 |
| dc.identifier.spa.fl_str_mv |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457696/ |
| dc.identifier.issn.spa.fl_str_mv |
2005-3606 / 2005-5447 (Electrónico) |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10554/54029 |
| dc.identifier.doi.spa.fl_str_mv |
https://dx.doi.org/10.15283%2Fijsc18061 |
| dc.identifier.instname.spa.fl_str_mv |
instname:Pontificia Universidad Javeriana |
| dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional - Pontificia Universidad Javeriana |
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repourl:https://repository.javeriana.edu.co |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457696/ http://hdl.handle.net/10554/54029 https://dx.doi.org/10.15283%2Fijsc18061 |
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2005-3606 / 2005-5447 (Electrónico) instname:Pontificia Universidad Javeriana reponame:Repositorio Institucional - Pontificia Universidad Javeriana repourl:https://repository.javeriana.edu.co |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.citationstartpage.spa.fl_str_mv |
51 |
| dc.relation.citationendpage.spa.fl_str_mv |
62 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
International Journal of Stem Cells |
| dc.relation.citationvolume.spa.fl_str_mv |
12 |
| dc.relation.citationissue.spa.fl_str_mv |
1 |
| dc.rights.licence.*.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
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http://creativecommons.org/licenses/by-nc/4.0/ |
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Atribución-NoComercial 4.0 Internacional http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
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PDF |
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Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2Mejía-Cruz, Claudia CamilaBarreto-Durán, EmiliaPardo-Pérez, María AlejandraJimenez, María CamilaRincón, JuliethVanegas, KarenRodríguez, Jorge LuisJaramillo-Garcia, Luis FernandoUlloa, Juan CarlosMartínez Díaz, RodolfoLeal-García, EfrainPérez-Núñez, RafaelBarreto, AlfonsoRodríguez-Pardo, Viviana MPontificia Universidad Javeriana. Facultad de Medicina. Departamento de Ginecología y Obstetricia2021-06-10T00:20:31Z2021-06-10T00:20:31Z2019https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457696/2005-3606 / 2005-5447 (Electrónico)http://hdl.handle.net/10554/54029https://dx.doi.org/10.15283%2Fijsc18061instname:Pontificia Universidad Javerianareponame:Repositorio Institucional - Pontificia Universidad Javerianarepourl:https://repository.javeriana.edu.coPDFapplication/pdfengBORRARGeneration of Organotypic Multicellular Spheres by Magnetic Levitation : Model for the Study of Human Hematopoietic Stem Cells MicroenvironmentArtículo de revistahttp://purl.org/coar/resource_type/c_beb9Q4Background and Objective The characteristics of human hematopoietic stem cells are conditioned by the microenvironment of the bone marrow, where they interact with other cell populations, such as mesenchymal stem cells and endothelial cells; however, the study of this microenvironment is complex. The objective of this work was to develop a 3D culture system by magnetic levitation that imitates the microenvironment of human HSC. Methods and Results Human bone marrow-mesenchymal stem cells, umbilical cord blood-hematopoietic stem cells and a non-tumoral endothelial cell line (CC2811, Lonza®) were used to develop organotypic multicellular spheres by the magnetic levitation method. We obtained viable structures with an average sphericity index greater than 0.6, an average volume of 0.5 mm3 and a percentage of aggregation greater than 70%. Histological studies of the organotypic multicellular spheres used hematoxylin and eosin stains, and an evaluation of vimentin expression by means of immunohistochemistry demonstrated an organized internal structure without picnotic cells and a high expression of vimentin. The functional capacity of human hematopoietic stem cells after organotypic multicellular spheres culture was evaluated by multipotency tests, and it was demonstrated that 3D structures without exogenous Flt3L are autonomous in the maintenance of multipotency of human hematopoietic stem cells. Conclusions We developed organotypic multicellular spheres from normal human cells that mimic the microenvironment of the human hematopoietic stem cells. These structures are the prototype for the development of complex organoids that allow the further study of the biology of normal human stem cells and their potential in regenerative medicine.https://orcid.org/0000-0002-9152-5552Revista Internacional - IndexadaOrganotypic multicellular spheresOrganoidsHematopoietic stem cellsMesenchymal stem cellsMicroenvironment5162International Journal of Stem Cells121LICENSElicense.txtlicense.txttext/plain; charset=utf-82603http://repository.javeriana.edu.co/bitstream/10554/54029/2/license.txt2070d280cc89439d983d9eee1b17df53MD52open accessORIGINALijsc-12-051.pdfijsc-12-051.pdfArtículoapplication/pdf4934391http://repository.javeriana.edu.co/bitstream/10554/54029/1/ijsc-12-051.pdfb18d66a34d49d6916011a45893b7f8bcMD51open accessTHUMBNAILijsc-12-051.pdf.jpgijsc-12-051.pdf.jpgIM Thumbnailimage/jpeg8243http://repository.javeriana.edu.co/bitstream/10554/54029/3/ijsc-12-051.pdf.jpg0b88f92744a9873b295c44c5bbf13c59MD53open access10554/54029oai:repository.javeriana.edu.co:10554/540292021-07-14 16:48:53.365Repositorio Institucional - Pontificia Universidad Javerianarepositorio@javeriana.edu.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 |