CARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁ

Using an ELISA test, camation mottle virus (CarMV) was found to infect 82% of the camation samples collected from the Sabana of Bogotá. The isolated virus was mechanically inoculated on virus-free carnation plants and purified by sucrose gradient centrifugation. CarMV coat protein was analized by SD...

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Autores:
Rodríguez Sáenz, Anayervid; Departamento de Microbiología Facultad de Ciencias Pontificia Universidad Javeriana
Acosta Losada, Orlando; Unidad de Bioquímica Instituto de Biotecnología Universidad Nacional de Colombia, Bogotá
Peñaranda, José; Unidad de Bioquímica Instituto de Biotecnología Universidad Nacional de Colombia, Bogotá
Tipo de recurso:
Article of journal
Fecha de publicación:
2013
Institución:
Pontificia Universidad Javeriana
Repositorio:
Repositorio Universidad Javeriana
Idioma:
eng
OAI Identifier:
oai:repository.javeriana.edu.co:10554/31014
Acceso en línea:
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4821
http://hdl.handle.net/10554/31014
Palabra clave:
null
cápside viral, carmovirus, clavel, dsRNA, electroforesis.
null
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openAccess
License
Atribución-NoComercial-SinDerivadas 4.0 Internacional
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network_name_str Repositorio Universidad Javeriana
repository_id_str
spelling Atribución-NoComercial-SinDerivadas 4.0 Internacionalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2nullRodríguez Sáenz, Anayervid; Departamento de Microbiología Facultad de Ciencias Pontificia Universidad JaverianaAcosta Losada, Orlando; Unidad de Bioquímica Instituto de Biotecnología Universidad Nacional de Colombia, BogotáPeñaranda, José; Unidad de Bioquímica Instituto de Biotecnología Universidad Nacional de Colombia, Bogotá2018-02-24T15:59:00Z2020-04-15T18:09:35Z2018-02-24T15:59:00Z2020-04-15T18:09:35Z2013-05-17http://revistas.javeriana.edu.co/index.php/scientarium/article/view/48212027-13520122-7483http://hdl.handle.net/10554/31014Using an ELISA test, camation mottle virus (CarMV) was found to infect 82% of the camation samples collected from the Sabana of Bogotá. The isolated virus was mechanically inoculated on virus-free carnation plants and purified by sucrose gradient centrifugation. CarMV coat protein was analized by SDS-PAGE showing a single component of about 38 Kda. Double-stranded RNA (dsRNA) extracted from carnation infected tissue showed 3 to 5 electrophoretic components ranging between 8.0 and 0.9 Kbp. Virus particles contained a genomic RNA of about 4.0 Kbp as revealed by agarosa gel electrophoresis. It was posible to distinguished 2 groups of electrophoretic pattems using RNAse T1 digestion of the RNA contained in the CarMV purified particles.PDFapplication/pdfengPontificia Universidad Javerianahttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/4821/3701Universitas Scientiarum; Vol 5, No 1 (2000); 7-17Universitas Scientiarum; Vol 5, No 1 (2000); 7-17Universitas Scientiarum; Vol 5, No 1 (2000); 7-17nullcápside viral, carmovirus, clavel, dsRNA, electroforesis.nullCARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁnullnullnullhttp://purl.org/coar/version/c_970fb48d4fbd8a85Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/article10554/31014oai:repository.javeriana.edu.co:10554/310142023-03-28 16:16:00.071Repositorio Institucional - Pontificia Universidad Javerianarepositorio@javeriana.edu.co
dc.title.spa.fl_str_mv CARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁ
title CARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁ
spellingShingle CARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁ
null
cápside viral, carmovirus, clavel, dsRNA, electroforesis.
null
title_short CARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁ
title_full CARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁ
title_fullStr CARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁ
title_full_unstemmed CARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁ
title_sort CARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁ
dc.creator.fl_str_mv Rodríguez Sáenz, Anayervid; Departamento de Microbiología Facultad de Ciencias Pontificia Universidad Javeriana
Acosta Losada, Orlando; Unidad de Bioquímica Instituto de Biotecnología Universidad Nacional de Colombia, Bogotá
Peñaranda, José; Unidad de Bioquímica Instituto de Biotecnología Universidad Nacional de Colombia, Bogotá
dc.contributor.author.none.fl_str_mv Rodríguez Sáenz, Anayervid; Departamento de Microbiología Facultad de Ciencias Pontificia Universidad Javeriana
Acosta Losada, Orlando; Unidad de Bioquímica Instituto de Biotecnología Universidad Nacional de Colombia, Bogotá
Peñaranda, José; Unidad de Bioquímica Instituto de Biotecnología Universidad Nacional de Colombia, Bogotá
dc.contributor.none.fl_str_mv null
dc.subject.spa.fl_str_mv null
cápside viral, carmovirus, clavel, dsRNA, electroforesis.
null
topic null
cápside viral, carmovirus, clavel, dsRNA, electroforesis.
null
description Using an ELISA test, camation mottle virus (CarMV) was found to infect 82% of the camation samples collected from the Sabana of Bogotá. The isolated virus was mechanically inoculated on virus-free carnation plants and purified by sucrose gradient centrifugation. CarMV coat protein was analized by SDS-PAGE showing a single component of about 38 Kda. Double-stranded RNA (dsRNA) extracted from carnation infected tissue showed 3 to 5 electrophoretic components ranging between 8.0 and 0.9 Kbp. Virus particles contained a genomic RNA of about 4.0 Kbp as revealed by agarosa gel electrophoresis. It was posible to distinguished 2 groups of electrophoretic pattems using RNAse T1 digestion of the RNA contained in the CarMV purified particles.
publishDate 2013
dc.date.created.none.fl_str_mv 2013-05-17
dc.date.accessioned.none.fl_str_mv 2018-02-24T15:59:00Z
2020-04-15T18:09:35Z
dc.date.available.none.fl_str_mv 2018-02-24T15:59:00Z
2020-04-15T18:09:35Z
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.hasversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.local.spa.fl_str_mv Artículo de revista
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
format http://purl.org/coar/resource_type/c_6501
dc.identifier.none.fl_str_mv http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4821
dc.identifier.issn.none.fl_str_mv 2027-1352
0122-7483
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10554/31014
url http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4821
http://hdl.handle.net/10554/31014
identifier_str_mv 2027-1352
0122-7483
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.uri.none.fl_str_mv http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4821/3701
dc.relation.citationissue.eng.fl_str_mv Universitas Scientiarum; Vol 5, No 1 (2000); 7-17
dc.relation.citationissue.spa.fl_str_mv Universitas Scientiarum; Vol 5, No 1 (2000); 7-17
dc.relation.citationissue.por.fl_str_mv Universitas Scientiarum; Vol 5, No 1 (2000); 7-17
dc.rights.licence.*.fl_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.spa.fl_str_mv PDF
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv null
null
null
dc.publisher.eng.fl_str_mv Pontificia Universidad Javeriana
institution Pontificia Universidad Javeriana
repository.name.fl_str_mv Repositorio Institucional - Pontificia Universidad Javeriana
repository.mail.fl_str_mv repositorio@javeriana.edu.co
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