CARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁ
Using an ELISA test, camation mottle virus (CarMV) was found to infect 82% of the camation samples collected from the Sabana of Bogotá. The isolated virus was mechanically inoculated on virus-free carnation plants and purified by sucrose gradient centrifugation. CarMV coat protein was analized by SD...
- Autores:
-
Rodríguez Sáenz, Anayervid; Departamento de Microbiología Facultad de Ciencias Pontificia Universidad Javeriana
Acosta Losada, Orlando; Unidad de Bioquímica Instituto de Biotecnología Universidad Nacional de Colombia, Bogotá
Peñaranda, José; Unidad de Bioquímica Instituto de Biotecnología Universidad Nacional de Colombia, Bogotá
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2013
- Institución:
- Pontificia Universidad Javeriana
- Repositorio:
- Repositorio Universidad Javeriana
- Idioma:
- eng
- OAI Identifier:
- oai:repository.javeriana.edu.co:10554/31014
- Acceso en línea:
- http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4821
http://hdl.handle.net/10554/31014
- Palabra clave:
- null
cápside viral, carmovirus, clavel, dsRNA, electroforesis.
null
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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Atribución-NoComercial-SinDerivadas 4.0 Internacionalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2nullRodríguez Sáenz, Anayervid; Departamento de Microbiología Facultad de Ciencias Pontificia Universidad JaverianaAcosta Losada, Orlando; Unidad de Bioquímica Instituto de Biotecnología Universidad Nacional de Colombia, BogotáPeñaranda, José; Unidad de Bioquímica Instituto de Biotecnología Universidad Nacional de Colombia, Bogotá2018-02-24T15:59:00Z2020-04-15T18:09:35Z2018-02-24T15:59:00Z2020-04-15T18:09:35Z2013-05-17http://revistas.javeriana.edu.co/index.php/scientarium/article/view/48212027-13520122-7483http://hdl.handle.net/10554/31014Using an ELISA test, camation mottle virus (CarMV) was found to infect 82% of the camation samples collected from the Sabana of Bogotá. The isolated virus was mechanically inoculated on virus-free carnation plants and purified by sucrose gradient centrifugation. CarMV coat protein was analized by SDS-PAGE showing a single component of about 38 Kda. Double-stranded RNA (dsRNA) extracted from carnation infected tissue showed 3 to 5 electrophoretic components ranging between 8.0 and 0.9 Kbp. Virus particles contained a genomic RNA of about 4.0 Kbp as revealed by agarosa gel electrophoresis. It was posible to distinguished 2 groups of electrophoretic pattems using RNAse T1 digestion of the RNA contained in the CarMV purified particles.PDFapplication/pdfengPontificia Universidad Javerianahttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/4821/3701Universitas Scientiarum; Vol 5, No 1 (2000); 7-17Universitas Scientiarum; Vol 5, No 1 (2000); 7-17Universitas Scientiarum; Vol 5, No 1 (2000); 7-17nullcápside viral, carmovirus, clavel, dsRNA, electroforesis.nullCARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁnullnullnullhttp://purl.org/coar/version/c_970fb48d4fbd8a85Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/article10554/31014oai:repository.javeriana.edu.co:10554/310142023-03-28 16:16:00.071Repositorio Institucional - Pontificia Universidad Javerianarepositorio@javeriana.edu.co |
dc.title.spa.fl_str_mv |
CARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁ |
title |
CARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁ |
spellingShingle |
CARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁ null cápside viral, carmovirus, clavel, dsRNA, electroforesis. null |
title_short |
CARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁ |
title_full |
CARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁ |
title_fullStr |
CARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁ |
title_full_unstemmed |
CARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁ |
title_sort |
CARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁ |
dc.creator.fl_str_mv |
Rodríguez Sáenz, Anayervid; Departamento de Microbiología Facultad de Ciencias Pontificia Universidad Javeriana Acosta Losada, Orlando; Unidad de Bioquímica Instituto de Biotecnología Universidad Nacional de Colombia, Bogotá Peñaranda, José; Unidad de Bioquímica Instituto de Biotecnología Universidad Nacional de Colombia, Bogotá |
dc.contributor.author.none.fl_str_mv |
Rodríguez Sáenz, Anayervid; Departamento de Microbiología Facultad de Ciencias Pontificia Universidad Javeriana Acosta Losada, Orlando; Unidad de Bioquímica Instituto de Biotecnología Universidad Nacional de Colombia, Bogotá Peñaranda, José; Unidad de Bioquímica Instituto de Biotecnología Universidad Nacional de Colombia, Bogotá |
dc.contributor.none.fl_str_mv |
null |
dc.subject.spa.fl_str_mv |
null cápside viral, carmovirus, clavel, dsRNA, electroforesis. null |
topic |
null cápside viral, carmovirus, clavel, dsRNA, electroforesis. null |
description |
Using an ELISA test, camation mottle virus (CarMV) was found to infect 82% of the camation samples collected from the Sabana of Bogotá. The isolated virus was mechanically inoculated on virus-free carnation plants and purified by sucrose gradient centrifugation. CarMV coat protein was analized by SDS-PAGE showing a single component of about 38 Kda. Double-stranded RNA (dsRNA) extracted from carnation infected tissue showed 3 to 5 electrophoretic components ranging between 8.0 and 0.9 Kbp. Virus particles contained a genomic RNA of about 4.0 Kbp as revealed by agarosa gel electrophoresis. It was posible to distinguished 2 groups of electrophoretic pattems using RNAse T1 digestion of the RNA contained in the CarMV purified particles. |
publishDate |
2013 |
dc.date.created.none.fl_str_mv |
2013-05-17 |
dc.date.accessioned.none.fl_str_mv |
2018-02-24T15:59:00Z 2020-04-15T18:09:35Z |
dc.date.available.none.fl_str_mv |
2018-02-24T15:59:00Z 2020-04-15T18:09:35Z |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.hasversion.none.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.local.spa.fl_str_mv |
Artículo de revista |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
format |
http://purl.org/coar/resource_type/c_6501 |
dc.identifier.none.fl_str_mv |
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4821 |
dc.identifier.issn.none.fl_str_mv |
2027-1352 0122-7483 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10554/31014 |
url |
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4821 http://hdl.handle.net/10554/31014 |
identifier_str_mv |
2027-1352 0122-7483 |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.relation.uri.none.fl_str_mv |
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4821/3701 |
dc.relation.citationissue.eng.fl_str_mv |
Universitas Scientiarum; Vol 5, No 1 (2000); 7-17 |
dc.relation.citationissue.spa.fl_str_mv |
Universitas Scientiarum; Vol 5, No 1 (2000); 7-17 |
dc.relation.citationissue.por.fl_str_mv |
Universitas Scientiarum; Vol 5, No 1 (2000); 7-17 |
dc.rights.licence.*.fl_str_mv |
Atribución-NoComercial-SinDerivadas 4.0 Internacional |
dc.rights.accessrights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.coar.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
rights_invalid_str_mv |
Atribución-NoComercial-SinDerivadas 4.0 Internacional http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.spa.fl_str_mv |
PDF |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
null null null |
dc.publisher.eng.fl_str_mv |
Pontificia Universidad Javeriana |
institution |
Pontificia Universidad Javeriana |
repository.name.fl_str_mv |
Repositorio Institucional - Pontificia Universidad Javeriana |
repository.mail.fl_str_mv |
repositorio@javeriana.edu.co |
_version_ |
1808389760888078336 |