3D multicellular spheroid for the study of human hematopoietic stem cells : synergistic effect between oxygen levels, mesenchymal stromal cells and endothelial cells
Q3
- Autores:
-
Barreto Duran, Emilia
Jaramillo García, Luis Fernando
Mejía Cruz, Claudia Camila
Leal García, Efraín
Barreto Prieto, Alfonso
Rodríguez Pardo, Viviana Marcela
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2021
- Institución:
- Pontificia Universidad Javeriana
- Repositorio:
- Repositorio Universidad Javeriana
- Idioma:
- eng
- OAI Identifier:
- oai:repository.javeriana.edu.co:10554/59764
- Acceso en línea:
- https://pubmed.ncbi.nlm.nih.gov/34234608/#affiliation-1
http://hdl.handle.net/10554/59764
https://doi.org/10.2147/JBM.S305319
- Palabra clave:
- Hematopoietic stem cells
Niche
Bone marrow
Microenvironment
3D culture
- Rights
- License
- Atribución-NoComercial 4.0 Internacional
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| dc.title.spa.fl_str_mv |
3D multicellular spheroid for the study of human hematopoietic stem cells : synergistic effect between oxygen levels, mesenchymal stromal cells and endothelial cells |
| title |
3D multicellular spheroid for the study of human hematopoietic stem cells : synergistic effect between oxygen levels, mesenchymal stromal cells and endothelial cells |
| spellingShingle |
3D multicellular spheroid for the study of human hematopoietic stem cells : synergistic effect between oxygen levels, mesenchymal stromal cells and endothelial cells Hematopoietic stem cells Niche Bone marrow Microenvironment 3D culture |
| title_short |
3D multicellular spheroid for the study of human hematopoietic stem cells : synergistic effect between oxygen levels, mesenchymal stromal cells and endothelial cells |
| title_full |
3D multicellular spheroid for the study of human hematopoietic stem cells : synergistic effect between oxygen levels, mesenchymal stromal cells and endothelial cells |
| title_fullStr |
3D multicellular spheroid for the study of human hematopoietic stem cells : synergistic effect between oxygen levels, mesenchymal stromal cells and endothelial cells |
| title_full_unstemmed |
3D multicellular spheroid for the study of human hematopoietic stem cells : synergistic effect between oxygen levels, mesenchymal stromal cells and endothelial cells |
| title_sort |
3D multicellular spheroid for the study of human hematopoietic stem cells : synergistic effect between oxygen levels, mesenchymal stromal cells and endothelial cells |
| dc.creator.fl_str_mv |
Barreto Duran, Emilia Jaramillo García, Luis Fernando Mejía Cruz, Claudia Camila Leal García, Efraín Barreto Prieto, Alfonso Rodríguez Pardo, Viviana Marcela |
| dc.contributor.author.none.fl_str_mv |
Barreto Duran, Emilia Jaramillo García, Luis Fernando Mejía Cruz, Claudia Camila Leal García, Efraín Barreto Prieto, Alfonso Rodríguez Pardo, Viviana Marcela |
| dc.contributor.corporatename.spa.fl_str_mv |
Pontificia Universidad Javeriana. Facultad de Medicina. Departamento de Ortopedia y Traumatología Pontificia Universidad Javeriana. Facultad de Medicina. Hospital Universitario San Ignacio |
| dc.subject.keyword.spa.fl_str_mv |
Hematopoietic stem cells Niche Bone marrow Microenvironment 3D culture |
| topic |
Hematopoietic stem cells Niche Bone marrow Microenvironment 3D culture |
| description |
Q3 |
| publishDate |
2021 |
| dc.date.created.none.fl_str_mv |
2021-06-30 |
| dc.date.accessioned.none.fl_str_mv |
2022-05-12T12:29:22Z |
| dc.date.available.none.fl_str_mv |
2022-05-12T12:29:22Z |
| dc.type.local.spa.fl_str_mv |
Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.identifier.spa.fl_str_mv |
https://pubmed.ncbi.nlm.nih.gov/34234608/#affiliation-1 |
| dc.identifier.issn.spa.fl_str_mv |
1179-2736 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10554/59764 |
| dc.identifier.doi.spa.fl_str_mv |
https://doi.org/10.2147/JBM.S305319 |
| dc.identifier.instname.spa.fl_str_mv |
instname:Pontificia Universidad Javeriana |
| dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional - Pontificia Universidad Javeriana |
| dc.identifier.repourl.spa.fl_str_mv |
repourl:https://repository.javeriana.edu.co |
| url |
https://pubmed.ncbi.nlm.nih.gov/34234608/#affiliation-1 http://hdl.handle.net/10554/59764 https://doi.org/10.2147/JBM.S305319 |
| identifier_str_mv |
1179-2736 instname:Pontificia Universidad Javeriana reponame:Repositorio Institucional - Pontificia Universidad Javeriana repourl:https://repository.javeriana.edu.co |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.citationstartpage.spa.fl_str_mv |
517 |
| dc.relation.citationendpage.spa.fl_str_mv |
528 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Journal of Blood Medicine |
| dc.relation.citationvolume.spa.fl_str_mv |
12 |
| dc.rights.licence.*.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
| dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
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http://purl.org/coar/access_right/c_abf2 |
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Atribución-NoComercial 4.0 Internacional http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
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PDF |
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application/pdf |
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Pontificia Universidad Javeriana |
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Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2Barreto Duran, EmiliaJaramillo García, Luis FernandoMejía Cruz, Claudia CamilaLeal García, EfraínBarreto Prieto, AlfonsoRodríguez Pardo, Viviana MarcelaPontificia Universidad Javeriana. Facultad de Medicina. Departamento de Ortopedia y TraumatologíaPontificia Universidad Javeriana. Facultad de Medicina. Hospital Universitario San Ignacio2022-05-12T12:29:22Z2022-05-12T12:29:22Z2021-06-30https://pubmed.ncbi.nlm.nih.gov/34234608/#affiliation-11179-2736http://hdl.handle.net/10554/59764https://doi.org/10.2147/JBM.S305319instname:Pontificia Universidad Javerianareponame:Repositorio Institucional - Pontificia Universidad Javerianarepourl:https://repository.javeriana.edu.coPDFapplication/pdfeng3D multicellular spheroid for the study of human hematopoietic stem cells : synergistic effect between oxygen levels, mesenchymal stromal cells and endothelial cellsArtículo de revistahttp://purl.org/coar/resource_type/c_2df8fbb1Q3Q3Introduction: The human bone marrow microenvironment is composed of biological, chemical and physical factors that act in a synergistic way to modulate hematopoietic stem cell biology, such as mesenchymal stromal cells (MSCs), endothelial cells (ECs) and low oxygen levels; however, it is difficult to mimic this human microenvironment in vitro. Methods: In this work, we developed 3D multicellular spheroid (3D-MS) for the study of human hematopoietic stem cells (HSCs) with some components of perivascular niche. HSCs were isolated from umbilical cord blood, MSCs were isolated from human bone marrow and a microvasculature EC line (CC-2811, Lonza®) was used. For the formation of a 3D structure, a magnetic levitation culture system was used. Cultures were maintained in 21%, 3% and 1% O2 for 15 days. Culture volume, sphericity index and cell viability were determined. Also, human HSC proliferation, phenotype and production of reactive oxygen species were evaluated. Results: After 15 days, 3D-MS exhibited viability greater than 80%. Histology results showed structures without necrotic centers, and higher cellular proliferation with 3% O2. An increase in the expression of the CD34 antigen and other hematopoietic antigens were observed to 1% O2 with MSCs plus ECs and low ROS levels. Conclusion: These findings suggest that 3D-MS formed by MSCs, ECs and HSCs exposed to low concentrations of oxygen (1–3% O2) modulate human HSC behavior and mimics some features of the perivascular niche, which could reduce the use of animal models and deepen the relationship between the microenvironment of HSC and human hematological diseases development.https://orcid.org/ 0000-0002-9152-5552Revista Internacional - IndexadaSiHematopoietic stem cellsNicheBone marrowMicroenvironment3D culture517528Journal of Blood Medicine12ORIGINALJBM-305319-3d-multicellular-spheroid-for-the-study-of-human-hematopoiet.pdfJBM-305319-3d-multicellular-spheroid-for-the-study-of-human-hematopoiet.pdfArtículoapplication/pdf1114697http://repository.javeriana.edu.co/bitstream/10554/59764/1/JBM-305319-3d-multicellular-spheroid-for-the-study-of-human-hematopoiet.pdf076292f9173ae9662bfa64b6a52a891aMD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-82603http://repository.javeriana.edu.co/bitstream/10554/59764/2/license.txt2070d280cc89439d983d9eee1b17df53MD52open accessTHUMBNAILJBM-305319-3d-multicellular-spheroid-for-the-study-of-human-hematopoiet.pdf.jpgJBM-305319-3d-multicellular-spheroid-for-the-study-of-human-hematopoiet.pdf.jpgIM Thumbnailimage/jpeg10748http://repository.javeriana.edu.co/bitstream/10554/59764/3/JBM-305319-3d-multicellular-spheroid-for-the-study-of-human-hematopoiet.pdf.jpge8d37a60dc8e2fc9769f9df03214730eMD53open access10554/59764oai:repository.javeriana.edu.co:10554/597642022-05-13 03:04:44.121Repositorio Institucional - Pontificia Universidad Javerianarepositorio@javeriana.edu.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 |